9. 11 - It works by pengtt

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									Background: viral infection
To Here, 9.11.10
Micro -> Macro?
With plaques.
I. Mapping the genome of a virus
I. Mapping the genome of a virus



h clearness (here-
     color) of plaque
r size of plaque
I. Mapping the genome of a virus




                                        (h+ r+) + (h- r-)
                                   RF =
                                         total plaques
II. Transduction, and mapping of the Bacterial genome




IIa. Generalized Transduction
IIa. Generalized Transduction




     Score co-transductants , derive order and relative distance

            thr                     leu                 leu        azi
                                                azi     leu


                              thr                      leu azi
IIa. Specialized Transduction: Via a phage that integrates at ONE site



                                   site-specific recombination
                                   NOT homologous recombination
IIa. Specialized Transduction: Via a phage that integrates at ONE site
IIa. Specialized Transduction: Via a phage that integrates in one site
F’ - duction
F’




F-


     F’-duction
         Molecular Genetics
First step: DNA is the genetic material •

 DNA, NOT protein of the chromosomes/chromatin –



PROVEN WITH TRANSFORMATION AND –
TRANSDUCTION, THAT WE JUST MASTERED
DNA is the genetic material: Griffith - transformation
DNA is the genetic material: Avery et al. - transformation
DNA is the genetic material: Avery et al. - transformation
DNA is the genetic material: Hershey & Chase - transduction
         Genetic map




Physical map (open reading frames – gene sequences)
                                                          In vitro




NOT like above, where the DNA must recombine and replace the endogenous copy,
BUT where the DNA is extrachromosomal and persists as an episome (plasmid, F’, etc.)
                                                                      Also called
                                                                      “rescue”a-tr




                             Lyse bacteria, collect the multiple
                             Copies of plasmid DNA
                                    -Sequence the DNA
                                             of the inserted gene B


NOT like above, where the DNA must recombine and replace the endogenous copy,
BUT where the DNA is extrachromosomal and persists as an episome (plasmid, F’, etc.)
         Genetic map




Physical map (open reading frames – gene sequences)

								
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