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					                               Standard Operating Procedures
                                           Laboratory Specific
                               Chemical: Bromo-Deoxyuridine            (BrdU)
                     Please fill out the form completely. Print a copy and insert into your
                           Laboratory Safety Manual and Chemical Hygiene Plan.
                                       Refer to instructions for assistance.

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Department:________________________                          Date when SOP was written:_______

Date when SOP was approved by the lab supervisor: ___________________

Principal Investigator:___________________________________________________

Internal Laboratory Safety Coordinator/Lab Manager:___________________________________

Laboratory Phone:____________________ Office Phone:_____________________

Emergency Contact:____________________________________________________
                      (Name and Phone Number)

Location(s) covered by this SOP:__________________________________________
                                     (Building/Room Number)
_____________________________________________________________________________


Type of SOP:              Process         Hazardous Chemical             Hazardous Class


Purpose

Bromo-Deoxyuridine (BrdU) is used to label cells undergoing S-phase synthesis. It is a thymidine
analogue and gets incorporated in DNA. This SOP describes the safe usage and handling, and disposal
of BrdU.



Physical & Chemical Properties/Definition of Chemical Group
Chemical Name: 5-Bromo-2’-Deoxyuridine (BrdU)

CAS#:    59-14-3

Class: Reproductive toxin

Molecular formula:      C9H11BrN2O5

Molecular weight:        307.13 g

Form:          solid; white needles when crystallized from absolute
               ethanol; no distinctive odor




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Solubility:        Some solubility in water or saline
Volatility:        Stable dark and heat/humidity for 3 mos. at temperatures below 60°C. Sunlight causes
                   discoloration. Frozen aqueous solutions exposed to UV result in formation of debrominated
                   dimmer. Photooxidation occurs in air/light. BrdU is hydrolyzed at the N-glycosyl bond, this rate
                   increases at alkaline pH. Flammability restrictions do not apply.

Other:             Laboratory operations should be conducted in a fume hood
                   BrdU is a probable combustible compound. BrdU is cytotoxic, teratogenic, and mutagenic.
                   It is not carcinogenic; it is a topical antiviral agent and is useful in the treatment of
                   neoplasms and sensitizes tissue to X-rays.



Potential Hazards/Toxicity

Toxic Effects:                       BrdU is a toxic substance.

Acute Effects:                       Wheeze and cough, shortness of breath, burning in the mouth,
                                     throat, or chest.

Chronic Effects:                     Reproductive disorders and genetic alterations. BrdU is incorporated
                                     into tissue DNA in place of thymidine in animals. BrdU-substituted DNA
                                     replaces normal DNA and chromosomal proteins are altered through:
                                     chromosome lengthening, chromatid breakage, and changed sister
                                     chromatid exchange frequencies. Meiosis and mitosis are affected.

Carcinogenic Effects:                Brdu is suspected to be a carcinogen resulting in heritable genetic
                                     damage. It is harmful to the unborn child.

Mutagenic/Teratogenic Effects: BrdU is not mutagenic—Ames test; but BrdU is mutagenic—
                               micronucleus and sperm abnormality assay. BrdU is a strong teratogen
                               in rodents.

Systemic effects:                    BrdU integrates into DNA and affects the colon, stomach, bone marrow,
                                     and spleen.



Personal Protective Equipment (PPE)

Wear double nitrile gloves, full-length lab coat, and safety glasses/face shield in addition to long pants
and closed-toe shoes.

Always wash hands after removing gloves following handling BrdU.



Engineering Controls

Always handle BrdU inside a certified chemical fume hood or a ducted biosafety cabinet.



First Aid Procedures

Skin & Eye Exposure:        Skin: remove contaminated clothing and wash skin with soap and water; avoid
                            rubbing and increases in temperature. Seek medical attention promptly. Eye:



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                         rinse immediately with copious amounts of running water for at least 15 minutes.
                         Seek attention of a licensed Ophthalmologist promptly.

Ingestion:               Drink lots of water/milk. Induce vomiting. Seek medical attention (refer
                         gastric lavage).

Inhalation:             Remove rapidly to clean air. Administer rescue breathing if necessary
                        and call emergency services. Seek medical attention if needed.



Special Handling and Storage Requirements

       Whenever feasible, procedures with the potential for producing BrDu aerosols should be
        conducted with a certified chemical fume hood.

       Needles used for BrDu injection will be disposed of in approved sharps containers immediately
        following use.

       Needles used for BrDu injection should never be bent, sheared, or recapped.

Spill and Accident Procedure

       In case of eye exposure: go to eyewash station and rinse eyes for 15 minutes, then seek medical
        attention

       If inhaled: remove to fresh air, seek medical attention immediately

       If absorbed: through skin, immediately flush with plenty of water, remove contaminated shoes and
        clothing, seek medical attention immediately

       If ingested: Do NOT induce vomiting, loosen tight clothing, and seek medical attention
        immediately.

Chemical Spill Dial 911 and x59797

Spill – Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors.
Eliminate sources of ignition if the chemical is flammable. If possible, confine the spill to a small area
using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution
tape, barriers, etc.).

Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic
bags, label and take to the next chemical waste pick-up.

Large (>1 L) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for assistance.

Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency
shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S at x59797
immediately.




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Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor and EH&S at
x59797 immediately.



Medical Emergency Dial 911 or x52111

Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911 (or 310-
825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center (emergency room)
directly at x52111 (located at 757 Westwood Plaza, enter from Gayley Avenue). Note: All serious injuries
must be reported to EH&S at x59797 within 8 hours.

Non-Life Threatening Emergency– Go to the Occupational Health Facility (OHF), x56771, CHS
                              th       th
room 67-120 (This is on the 6 floor, 7 corridor, room 120. Enter through the School of Dentistry on
Tiverton Drive and proceed to the ―O‖ elevator to the 6th floor.)Hours: M - F, 7:30 a.m. to 4:30 p.m. At all
other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All serious
injuries must be reported to EH&S at x59797 within 8 hours.

Needle stick/puncture exposure (as applicable to chemical handling procedure)– Wash the
affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure,
flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing
231 from a campus phone, enter 93333 when prompted and then enter your extension. Hours: M – F,
8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room)
at x52111. Note: All needle stick/puncture exposures must be reported to EH&S at x59797 within 8 hours.



Decontamination/Waste Disposal Procedure

Clean areas where BrdU has been handled by adding water, followed with a soap and water wash.

No waste streams containing BrdU shall be disposed of in sinks or general refuse. Extra BrdU is
considered as hazardous chemical waste and disposed of in accordance with UCLA hazardous waste
protocol. Absorbent materials (e.g., associated with spill cleanup) grossly contaminated shall be handled
in accordance with the chemical waste disposal system.



Material Safety Data Sheet (MSDS) Location
(State the location of MSDS)
Hardcopy or electronic copy must be available.
Online MSDS can be accessed at http://msds.ehs.ucla.edu.



Protocol/Procedure
(Add specific description of procedure)




Note: Any deviation from this SOP requires written approval from PI.




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Documentation of Training (signature of all users is required)
   Prior to conducting any work with Bromo-deoxyuridine, designated personnel must provide training to
    his/her laboratory personnel specific to the hazards involved in working with this substance, work
    area decontamination, and emergency procedures.

   The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a
    copy of the Bromo-deoxyuridine MSDS provided by the manufacturer.

   The Principal Investigator must ensure that his/her laboratory personnel have attended appropriate
    laboratory safety training or refresher training within the last two years.

I have read and understand the content of this SOP:

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