Cells Organelle Biology

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Cells & Organelles Robert Hooke Demonstrator for Royal Society. During mid 1600’s lenses were a fad. Lecture 12 The Discovery of Cells Wanted to know why corks float. Looked at cork (plant) cells. Saw cells in 1665. Looked like monk’s “cells” Robert Hooke “If I had seen further, it is by standing on the shoulders of giants” From letter written by Sir Isaac Newton to Robert Hooke who was a dwarf. The Discovery of Cells Many men contributed to discovery: Antony van Leeuwenhoek: In 1678, discovered microscopic organisms. First to see bacteria and various protists. Animalcules. Animalcules Cell Theory Living organism made up of one or more cells. Basic functional unit. Arise from another or preexisting cells. Lecture 19 Contributors to Cell Theory: Theodore Schwann & Mathias Schleiden - 1839. All organisms composed of cells, the basic unit of life. The Cell Theory The Cell Theory Contributors to Cell Theory: Rudolph Virchow: Virchow Best known for his law - 1855: Omnis cellula e cellula, "every cell every originates from a pre-existing cell”. precell Eukaryotic Cell Components Cell membrane Cytoplasm Genetic material in nucleus Cell membrane: Cell boundary, keeps cell distinct from other cells. Cytoplasm: Content of cell inside : membrane excluding genetic material. Genetic material: Inside nucleus. Prokaryotic Cell Components Cell membrane Cytoplasm Genetic material in cytoplasm Genetic material: In cytoplasm, not enclosed in nucleus. Other differences. Prokaryotic Cells Nucleus absent. Plantae Fungi Animalia Some are Protista anaerobes. Monera Smaller cell size. Unicellular to filamentous. Organelles absent. absent Genetic material consists of one strand of DNA in a circle. Nucleus present. Aerobes. Cells larger than prokaryotic cell. Eukaryotic Cells Plantae Fungi Animalia Protista Monera Unicellular to multicellular. Organelles present. present Genetic material consists of chromosomes in a nucleus. Lecture 19 Two Types of Cells Cells are unifying theme for both types. Focus on eukaryotic cells. Monera: Prokaryotic kingdom. Protista, Plants, Animals and Fungi: Eukaryotic Kingdoms. Plantae Fungi Animalia Unifying Theme of Cells All cells are similar: Specialization occurs, but based on modification of existing structures. Cells are small, i.e., you need a microscope to see them. Small cells have increased membrane surface area. eukaryotic organisms prokaryotic organisms Protista Monera Cell Theory Expanded Volume to Surface Area 1950: electron microscope Total surface area 9 times greater. Different Identical Volumes Diffusion distances shorter in small cells. Process of Diffusion Diffusion: Spontaneous movement of matter or heat. Movement is from area of high density to lower density. The 27 smaller cells > surface area than 1 giant cell. Molecules must move in and out of cells! So What? Examples: Perfume in air, sugar in coffee. Much easier to diffuse in and out of smaller cell. Lecture 19 Significance of Small Cell Size Short diffusion distance is reason why cells are small and not large. Cells also similar in size. Some exceptions: Inside of egg is one cell. Other generalization: Plant cells larger than animals. Prokaryotic cells smaller than eukaryotic cells. Structural Similarities of Cells Surrounded by semisemipermeable membrane: membrane Acts like a sieve, selects what can get in and out of cell. Protein synthesized with same physical structures (=same organelles). Information for protein synthesis stored in DNA. Functional Similarities of Cells Energy source ATP. Mechanism of material transport through membrane. Conversion of information into protein. Cell division, i.e., one cell division divides to form another cell. Examples of Specialized Cells Epithelial cells (skin). Adipose cells (fat). Muscle cells (3 types). Nerve cells. Eggs & Sperm (unusual) Epithelial Cells Adipose Cells Lecture 19 Muscle and Nerve Cells Sperms Competing to Fertilize Egg Three Functional Systems Manufacturing proteins. proteins Metabolism: Converting Metabolism sugar to useful energy. Photosynthesis: Photosynthesis Capturing light energy. Manufacturing proteins Animal Cell Animal Cell vesicles cell membrane nucleus Organelles: Sub-Cellular Structures Protein production. Functions like a factory. Specialized “equipment”. golgi (ribosomes) endoplasmic reticulum (ER) Most surrounded by membrane. Protein Manufacturing Components Lecture 19 Engineers and Plans The Factory Engineering: Copy plans, send to factory. Factory assembles parts. Nucleus: Where Plans Are Nucleus and ER. Nucleus contains the master plans of the cell in the form of DNA. Nucleus: Where Plans Are When protein is needed Copy (=RNA) of part of DNA with RNA being info for assembled protein. DNA Factory: ER and Ribosomes RNA copy of plans for protein leaves nucleus. Plans go to factory = ribosome and ER. (ribosomes) endoplasmic reticulum (ER) Factory: ER and Ribosomes Protein is assembled in factory like an assembly line. Lecture 19 Factory: ER and Ribosomes Protein being assembled Amino acid Factory: ER and Ribosomes RNA RNA copy of “plan” Ribsome= worker proteins Ribosome moves RNA copy along assembly line conveyor belt so plan can be read. Finished proteins deposited inside ER. Finished Products Where to Now? Proteins sent into cytoplasm in form of secretory vesicles to the golgi. Packaged and “sent off” where needed. Golgi Apparatus Where to Now? Proteins sent into cytoplasm in form of vesicles to the golgi. Protein modification. From ER secretory vesicles Packaged in secretory vesicle. Lecture 19 Where to Now? Membrane bound structures with little or no structure. Plants: Fills with water and some organic compounds. Pressure from water expands cells. Plant Vacuoles Water entering cell growth Secretory vesicles now incorporated into cell. Allows cells to grow. Dehydrated and Hydrated Plants Storage Support Vacuoles Animal Water regulation Plant Dehydrated Plant Addition of water, filling cell’s vacuoles vacuole chloroplasts mitochondria Lecture 19

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