Exercise II

					Bio 211                                  A & P II                         Lab Exercises                  1

                              Biology 211 - A & P II
                              Laboratory Exercises
Required Materials and Safety Regulations for Bio 211 Labs:
Latex gloves: a box may be cheaper since you will need them ALL semester
Safety goggles or glasses: sunglasses are NOT acceptable
Colored pencils: the physical “act” of drawing the tissues is correlated with higher grades on the Bio 210 &
211 quizzes. In fact, the supplemental anatomy coloring book is an excellent means of learning the material;
the integration of vision and drawing reinforces the information.
Possibly closed-toed shoes: think of a dropping a scalpel and piercing your toe! For consistency and
safety, this may be required for every lab and quiz, even those in which a dissection is not scheduled.
Possibly a lab coat or lab apron: when purchasing, please keep in mind that you will also need a lab
coat for Bio 225 Lab – Microbiology lab


                                           Exercise II-1
                                       Endocrine System
Materials:
Lab 1:
Colored Pencils
Slides – specific slides listed on Objectives for Lab Quiz #1
Microscopes, transparencies, CD, and Web slides
Torso models and other models with endocrine glands
Lab 2:
Fetal Pig – container and solution for storage
Dissected cat
Dissection instruments and tray

References:
Atlas – refer to index for pages
Textbook

Instructions for Lab 1 & 2:
   During Lab 1, the lab instructor will review the location of the endocrine glands using models, Power
    Point slides (PPT) or transparencies and use the teaching microscope or PPT slides to identify pertinent
    regions, structures, features and hormones produced.
   The student will use their microscope to identify the regions, features and structures of the glands/organs
    as given on the Objectives for Lab Quiz #1 and sketch the tissue observed on the slides – space provided
    on the following pages. The student will identify the hormone produced by each region of the gland/organ.
   During Lab 2, the lab instructor will demonstrate & describe the procedure for opening the fetal pig before
    the student begins dissecting. The student is responsible for identifying glands/organs and the
    corresponding hormones on the fetal pig and the prosected cat as directed by the instructor.
   The instructor will inform the student of the procedure for preserving the pig, as it will be used for the
    remainder of the semester.
   The student will select an endocrine topic for Exercise II-4.

Questions/Notes: Describe the locations & appearance of the endocrine glands on fetal pig and cat.
Bio 211                                    A & P II                         Lab Exercises               2
Endocrine Glands and Hormones
ANTERIOR PITUITARY                1. _________________________        2. ____________________________________


3._______________________         4. ___________________________      5. ____________________________________


6. ______________________


POSTERIOR PITUITARY               1. ______________________   2. ___________________aka______________________
     (stored)


THYROID                           1. ______________________________         2. _______________________________


                                  3. ______________________________



PARATHYROID                       1. ______________________________



ADRENAL MEDULLA                   1. ______________________________ 2. _____________________________



ADRENAL CORTEX                    1. _________________________________________________
1-3) General class & a specific

Hormone                           2. _________________________________________________


                                  3. _________________________________________________


PANCREAS                          1. ______________________   2. ______________________


THYMUS                            1. ______________________   2. ______________________


PINEAL                            1. ______________________


OVARIES                           1. ______________________   2. ______________________


TESTES                            1. ______________________




Know the name and abbreviation for each hormone.
Bio 211                         A & P II   Lab Exercises   3

Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________
Bio 211                         A & P II   Lab Exercises   4

Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________
Bio 211                         A & P II   Lab Exercises   5

Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________
Bio 211                         A & P II   Lab Exercises   6

Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________
Bio 211                         A & P II   Lab Exercises   7

Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________
Bio 211                         A & P II   Lab Exercises   8

Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________
Bio 211                                 A & P II                          Lab Exercises                 9

Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




                                          Exercise II-2
                                                   Blood
Materials:
Slides – prepared blood slides & specific leukocyte slides
Microscopes
Blood Cell Poster

Additional Materials provided by student:
Colored pencils

References:
Atlas and textbook

Instructions:
      The lab instructor will review the types of blood cells and give their functions. The components of blood
       will be discussed with detail as given on the Objectives for Lab Quiz #1.
      The student will be able to identify the formed elements and specific leukocytes from blood slides and
       will sketch them on the following pages.
      The student will do a differential white blood cell count. Count approximately 50 leukocytes on the slide
       and specify the type leukocyte. Use the chart below and calculate the percentage of each. Compare
       this to the % expected from a normal blood sample (from text or instructor).

Questions/Notes:
Bio 211                               A & P II                Lab Exercises   10
Differential WBC:

   Leukocyte          # present         % of Total Counted   % Expected




Sketch the formed elements in blood

Erythrocytes
       Magnification: _____________
       Description:_______________
       _________________________
       _________________________




Platelets
       Magnification: _____________
       Description:_______________
       _________________________
       _________________________




Leukocyte:______________________
      Magnification: ______________
      Description:________________
      _________________________
      _________________________
Bio 211                         A & P II   Lab Exercises   11



Leukocyte:_____________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Leukocyte:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Leukocyte:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Leukocyte:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________
Bio 211                                 A & P II                          Lab Exercises                  12
                                           Exercise II-3
                                       Lymphatic System
Materials:
Slides – lymphatic vessel, lymph node, spleen, tonsils, ileum – Peyer’s Patchs
Microscopes
Torso model & head model & any models with lymph nodes

Additional Materials provided by student:
Colored pencils

References:
Atlas and textbook

Instructions:
      The lab instructor will review the location and functions of the lymphatic tissue referring to the
       Objectives for Lab Quiz #1.
      The student will locate the lymphatic tissue on models.
      The instructor will explain how to distinguish the various lymphatic slides. The student will use the
       microscope to find the lymphatic tissues listed in the objectives. Sketch these slides on the following
       pages.
      The student will be able to identify the locations of lymphatic tissue on the fetal pig and cat.

Questions/Notes:
Sketch the lymphatic tissues

Lymphatic Vessel
     Magnification: _____________
     Description:_______________
     _________________________
     _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________
Bio 211                         A & P II   Lab Exercises   13

Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________




Tissue:________________________
      Magnification: _____________
      Description:_______________
      _________________________
      _________________________
Bio 211                                  A & P II                          Lab Exercises                  14
                                            Exercise II-4
                        Endocrine Dysfunction Oral Report

            POSSIBLE ENDOCRINE DYSFUNCTION TOPICS
Addison’s Disease                            Cushing’s Syndrome
Hyperthyroidism:Graves’ Disease              Hyperthyroidism: Exophthalmic Goiter
Hypothyroidism: Cretinism                    Hypothyroidism: Myxedema
Simple Goiter (Endemic Goiter)               Diabetes Insipidus
Diabetes MellitusType I                      Diabetes Mellitus Type II
Giantism                                     Acromegaly
Dwarfism                                     SAD – seasonal associated disorder (melatonin)
Hormonal Infertility: Male                   Hormonal Infertility: Female
Adrenal virilism: Hirsutism                  Panhypopituitarism (Simmons’ Disease)
Hyperparathyriodism                          Hypoparathyroidism
Anabolic steroids - Medical usage            Erythropoietin usage by athletes
Growth hormone therapy                       Estrogen/Progesterone Replacement therapy
Testosterone Replacement therapy             Multiple Endocrine Neoplasia (MEN) cancers Type I & II
Hyperprolactinemia/Dopamine Antagonists      Androgen Abuse/ Anabolic steroid usage by athletes
GHB Use                                      General Adaptation Syndrome (GAS)
Pheochromocytoma

If you know of and are interested in an Endocrine Disorder that is not listed and you would like to write your
report on it, please ask your Instructor; it may be welcome.

Each student will select an Endocrine Dysfunction or hormone disorder either from the list or an approved
topic. Sign up with your instructor. This oral report will count as 4 points on the first lab quiz.

Name of disorder:



Description of disorder and symptoms (relate to the Endocrine System):




Identify the cause:




Treatment:

				
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