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Injection and Ultrafast Mixing of Attomole Samples Via Micro

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									      INJECTION AND ULTRAFAST MIXING OF
   ATTOMOLE SAMPLES VIA MICRO-NANOFLUIDIC
    GATES FOR ON-CHIP BIOCHEMICAL ANALYSIS
                    T-C. Kuo”~~), H-K. Kim(‘), D. M. Cannon (lv3),
 M. A. Shannon (2931,                                             Jr.,
      B. R. Flaehsbart(‘), J. V. Sweedler(1’31,and P. W. Bohn(“31
  Depar-tment of Chemisttyil~, Departmen t qf Mechanical and Industrial Engineering’2~, and
 Beckman Institutefor Advurzcedkience and Technology’3i, ~niv~~~i~ of Illinois at Urbnna-
                Champ&p, 600 South Mathews Ave., U&ma, Illinois 61801

ABSTRACT
      A three-dimensional   microfluidic circuit with nanofluidic interconnecting  gates has
been developed for the injection and extremely rapid mixing of analytes for chip-based
biochemical assays. Polycarbonate       nanoporous membranes containing 15- to 200-nm
diameter cylindrical pores are employed as electrically-controlled       molecular gates to
inject bands of ultrasmall analyte samples (-10 attomoles) from one microchannel
channel to another, and to completely mix and react analytes within microns of the
injection point. The molecular gating, injection, and rapid mixing functionalities depends
on the electrokinetic flow through the nanopores, which separates the microchannels.
Keywords: biochemical                     analysis, laminar flow injection, micro-mixing,                               nanofluidic

INTRODUCTION
     Injecting, mixing, and reacting analytes are important functions for the many chip-
based biochemical assays being developed1’-31. We report on the fabrication and testing
of a three-dimensional   microfluidic circuit with nanofluidic interconnecting gates that




Figure 1. (a) Top view of a micro-nanofluidic
bioassay chip where analytes are first separated
in a channel and bands are then injected into
another channel via nanofluidic membrane
sandwiched in between. (b) A close-up of the
injection point. (c> A side view of the stack,
with the interconnect layers bonded between
two glass layers, with a PDMS top layer to                                                                         PDnitSports layer
                                                                                                                           * .^
facilitate attaching inlet and outlet tubes.




                      7th lnternat~onal    Conference   on Miniaturized Chemical and Blochemlcal   Analysts   Systems
                                              October   5-9, 2003, Squaw Valley, Callfornla USA



O-974361 I-0.O/~TAS2003/$15.0002003TRF                                                                                                 5
allow injection and extremely rapid mixing of ultralow attomole (IO-r*) quantities of
analytes. Polycarbonate       nanoporous    membranes     containing    15 to 200 nm diameter
cylindrical pores are employed as electrically-controlled         non-moving gates to establish
fluidic communication      between microfluidic channels in vertically separated planes. The
nanofluidic pores in the regions of crossing microfluidic channels are electrokinetically
addressed, enabling these nanofluidic         gates to determine flow based upon chemical
composition,       molecular      size, or electrophoretic        mobility[4-61. This   hybrid
microfluidic/nanofluidi~     architecture allows ultrasmall analyte samples (thus far down to
tens of attomoles) to be separated in one channel, single bands to be injected and
collected into another channel with near 100% mass efficiencyt7), and then completely
mixed and reacted within microns of the injection point.

RESULTS     AND DISCUSSION
         Figure 1 shows a glass-based separation and injection chip consisting of a stack
of patterned dissimilar materials: glass, polycarbonate   membranes, PDMS, and other
polymer layers. The bottom piece serves as the assembly platform and comprises a glass
substrate with standard electrophoresis    channels etched into it. To this substrate are
bonded alternating layers of polycarbonate    membranes with addressable nanopores and

                                    (a)                                                            (C‘l




                                      voltage pathways        used for   transport
                     separation                                                        injection
                           kind                                                             ivd




  Figure 2. (a) Schematic representation of injection channel connected to separation channel
  via nauofluidic membrane. (b) Electrical bias configurations for active electrokinetic
  injection control were repeated for serial separations with either manual or automated timed
  computer control. (c) Fluorescence image (captured from video) demonstrating the
  dependence of injection (membrane gate) bias on amount injected.




                7th lnternat~onal   Conference   on Miniaturized Chemical and Blochemlcal   Analysts   Systems
                                       October   5-9, 2003, Squaw Valley, Callfornla USA
polymer spacer layers with microfluidic channels etched in them. The crossed channels
visible in Fig. lb have polycarbonate nuclear-tracked    nanopore membranes (- 6-10 urn
thick) in between glass wafers, as shown in Fig. lc.            The crossed channels are
conceptually     shown in Fig. 2a to show the separation and injection channels.       By
controlling the voltage between the channels, electrokinetic     flows can induced within
each channel, and then across the nanopore member, as shown in Fig. 2b. As illustrated
in Fig. 2c, analyte can be selectively removed from the separation channel and injected
into the collection channel. For the nanopore membranes used, no measurable cross over
of analyte is observed without a bias applied across the membrane. As discussed in [7],
the electrokinetic   injection across the nanofluidic  gate occurs with near 100% mass
efficiency. Plus, extremely narrow bands of anal e can be removed from the separation
channel and injected into the collection channel Ii . Control of the analyte injection for
 15 nm pore membranes is on the order of 0.1 attomoles (lo-“) per volt, with a nearly
linear response until saturation occurs at about 200 V drop.
      In addition to exquisite control in the quantity of injected analyte, another notable
feature of the micro-nanofluidic    gate is ultrafast mixing of the analyte in the collection
channel. Figure 3 illustrates this ultrafast mixing by monitoring the Ca*+-CGD binding
reaction that can only occur when Ca*’ 1s injected into the collection channel. As the
fluorescence image in (a) and the intensity plot in (b) shows, the Ca binds completely
within the gating time, and well before fluid has traversed the width of the injection
channel, or about 30 urn. Note that the flow velocity in both channels, as well as through




 Figure 3. (a) Fluorescence   images of Ca*‘-CGD binding reaction. Left, membrane
 reverse bias. Right, membrane forward bias, Ca *+ is injected into the horizontal CGD
 channel.    (b) Fluorescence  intensity (left ordinate) and ap@ied bias state, (right
 ordinate, -.-) as a function of time showing transport of Ca       across 200 nm pore
 diameter PCTE membranes into the 2-uM CGD-containing         channel.   (c) Fluorescence
 intensity of CGD-Ca*+ in the receiving channel as a function of [Ca*‘].




               7th lnternat~onal   Conference   on Miniaturized Chemical and Blochemlcal   Analysts   Systems
                                      October   5-9, 2003, Squaw Valley, Callfornla USA



                                                                                                                7
the nanopore itself, is creeping, with Re << 1. The                                 * * oI     +     o
flow is highly laminar and under such conditions,                                   s j( D* * e B ’ B -”          “* **e *
                                                                                                                       a
complete mixing between the two microchannels
would be expected to occur by diffusion over many
millimeters.
      The mechanisms        for the ultrafast mixing are
still under investigation.       However, mixing in the
lateral direction due to the close spacing of the
nanopores      in the nuclear-tracked      membranes      is
                                                                                    . . -*;:
conceptually shown in Fig. 4. Diffusion between the
fluid leaving the pores and the surrounding           fluid    Figure 4. Cartoon of lateral
occurs on the order of microseconds,              thereby      diffusion    between      nanopores
laterally mixing the fluid streams within microns of           that are microns apart leading to
fluid travel in the lateral direction, depicted by the        rapid mixing of the top reactants
                                                               injected into the bottom stream.
dimension d. The average distance d is determined
by the pore density and ranges from - 0.1 to 10 pm. Interestingly, the distance x that the
mixing occurs over appears to be surprisingly large, on the order of many microns, for
electrokinetic    injection using the nanopore membranes.         At this time, we believe this
transverse mixing may be due to the high electric fields present at the nanopore exit,
creating electrophoretic      velocities high enough to effectively increase mixing in the x
direction. The result of both interactions is that the two streams completely mix within
microns of the intersection between the two crossed channels.
REFERENCES
[I] Salimi-Moosavi, H., T. Tang, and D.J. Harrison, “Electroosmotic pumping of organic solvents
    and reagents in microfabricated reactor chips,“J. Am. Chem. Sot. 1997 v. 119, p. 8716-8717.
[2] Jacobson, SC., T.E. M&night, and J.M. Ramsey, ‘Microfluidic devices for electrokinetically
    driven parallel and serial mixing,” Anal. Chem. 1999 v. 71, p. 4455-4459.
[3] Manz, A., C.S. Effenhauser,         N. Burggraf,   D.J. Harrison, K. Seiler, and K. Fluri,
      ‘“Electroosmotic pumping and electrophoretic separations for miniaturized chemical analysis
      systems, “J. Mcromech. Microerg 1994 v. 4, p. 257-265.
[4]   Kuo, T-C., L.A. Sloan, J.V. Sweedler, and P.W. Bohn, “Manipulating molecular transport
      through nanoporous membranes by control of electrokinetic flow: Effect of surface charge
      density and Debye length,” Langmuir 2001 v. 17 , p, 6298-6303.
[5]    Kuo, T-C.; D.M. Cannon, Jr., M.A. Shannon, J.V. Sweedler, and P.W. Bohn, “Hybrid three-
      dimensional nanofluidic/microfluidic  devices using molecular gates,” Sew. Aczrtators A, 2003
      v. 102 (3), p. 223-233.
[6]   Kuo, T-C., D.M. Cannon, Jr., W. Feng, M.A. Shannon, J.V. Svveedler, and P.W. Bohn,
      “Three-dimensional Ruidic architectures using nanofluidic diodes to control transport between
      microfluidic channels in micromechanical     devices,” in Micro Total Analysis Systems 2001,
      Ramsey, J.M. and van den Berg, A., eds., Kluwer Acad. Pub., 2001, p. 60-62.
[7]   Kuo, T-C., D.M. Cannon Jr., Y. Chen, J.J. Tulock, M.A. Shannon, J.V. Sweedler, and P.W.
      Bohn, “Gateable nanofluidic interconnects in multilevel microfluidic separation systems,”
      Anal. Chem., 2003 v. 75, p. 1861-1867.




                 7th lnternat~onal   Conference   on Miniaturized Chemical and Blochemlcal   Analysts   Systems
                                        October   5-9, 2003, Squaw Valley, Callfornla USA

								
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