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Trinity College School of Pharmacy Mass Spectrometry Requisition Form

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Trinity College School of Pharmacy Mass Spectrometry Requisition Form Powered By Docstoc
					       SCHOOL OF PHARMACY & PHARMACEUTICAL SCIENCES



        Mass Spectrometry Requisition Form

Test Guidelines

   Samples should be submitted in a 2mL HPLC vial

   Samples should be prepared to a concentration range 1-50µg/mL

   Samples should be prepared in a water-soluble organic solvent or solvent/water
    mix and filtered via 0.2u syringe filter. An acid or base to 0.1% v/v may also be
    added (see below)

   Compounds containing ionisable functional groups (i.e. amines or carboxylic
    acids) will generally ionize much easier than neutral compounds

   Ionisation can be enhanced by promoting protonation or deprotonation of the
    analyte. For example, addition of 0.1% Acetic acid to an analyte containing an
    amino functional group will protonate the amine, hence (M+H+) will be detected
    in positive ion mode. Addition of 0.1% NH4OH to an analyte containing an acidic
    functional group will deprotonate the acid, hence (M-H+) will be detected in
    negative ion mode.

   Only volatile buffers should be used if needed to prepare analyte, i.e. ammonia,
    acetic or formic acid. Avoid the use of non-volatile buffers i.e. phosphates,
    borate

   The use of Trifluoroacetic acid should be avoided

   Completed requisition forms should be handed to a technician along with the
    samples and also submitted via email to orbitrap@tcd.ie. The office is situated
    in Room 3.1 on the 3rd Floor of the School of Pharmacy & Pharmaceutical
    Sciences

   Results will be returned by email
         SCHOOL OF PHARMACY & PHARMACEUTICAL SCIENCES



             Mass Spectrometry Requisition Form

Submitted by:
Dept/Company:
Supervisor:
Phone No:                                             Date:

                                                                    Atmospheric Pressure
                            Electrospray Ionisation
                                                                     Chemical Ionisation
                          Positive          Negative              Positive         Negative
Testing required
 (Please Tick )




Sample Name(s)        Molecular Mass                  Molecular Formula i.e. C12H22NO3




Comments (if any):




Contact:
Brian Talbot (Technical Officer)
Paul Matthew O’Byrne M.Sc.
            rd
Room 3.1, 3 Floor,
                                                  Phone: +353 1 896 2852 / 2859
School of Pharmacy & Pharmaceutical Sciences
                                                  Fax:   +353 1 896 2821
Panoz Institute,
                                                  Email: orbitrap@tcd.ie
Trinity College, Dublin 2

				
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