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Cell Disruption Solutions Cell Disruption Solutions

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					                                                                     CONSTANT
                                                                       SYSTEMS LTD



                                         Cell Disruption Solutions




                                                     www.constantsystems.com




                                           cell disruption
              protein extraction


tissue disruption                        continuous processing


                                                       selective breakage

                     enzyme release

DNA manipulation                                        single cell isolation



              ISO 9001:2000 Registered
What our Customers                                       Say...
Pfizer - UK
  "We have been using the B-Series machine for 5 years now and find it extremely efficient at disrupting a wide range of micro
  organisms, from insect cells to the yeast Pichia Pastoris. I would definitely recommend it to the scientific community"

National Institute of Health - USA
  “We are very happy with the One Shot cell disrupter and find it to be very efficient, easy to use and gives reproducible
  results"

University of Cambridge, Department of Pharmcology - UK
  "The Basic Z gives us the high-quality bacterial cell extracts that we need for our research"

Tokyo University - Japan
  "Our One Shot model is highly effective and easily disrupts Fungi such as Aspergillus"

California Institute of Technology - USA
  "I am getting consistently good Iysates that are very concentrated and perform nicely."

Centre for Applied Microbiology and Research (CAMR), Porton Down - UK
  "The Constant Systems Cell disrupter has provided CAMR an invaluable resource we can offer to our customers who require
  contract Process development and GMP manufacturing"

Syngenta - UK
  "We purchased the 2.2kW cell disrupter from Constant Systems 6 months ago and we are very pleased with its speed and
  efficiency of disruption (E-Coli/Yeast). It has proved to be a very reliable machine! The training was comprehensive and
  effective. Well done!"

Astex Technology - UK
  "At Astex, the Constant Systems Cell Disrupter is mainly used for bacterial cells disruption (usually E. coli). Although it has
  additionally been used for insect and yeast cell Iysis. The disrupter gives good Iysis of E. coli. When insect cells are Iysed
  with the system the breakage is good"

SmithKline Beecham - Belgium
  "cooling system is efficient... quick and easy to use" "the quality of extracted proteins is superior"

University of Strathclyde - UK
  "robustly designed.. . easy to maintain" "the cell disrupter gave reproducible results... fast and reliable"

Vetinerary Laboratories - UK
  "quick and simple to perform, it did not generate a high temperature"

University of Wales, Swansea - UK
  "high levels of containment.. . effectively cooled" "easy to operate. .. easy control of the operating pressure"

Horseracing Forensic Laboratory - UK
  "easy to use and importantly quick and easy to clean "superior to current homogenisation methods for drug residue studies"

Scottish Crop Research Institute, Unit of Plant Biochemistry - UK
  "We have been extremely happy in our dealings with Constant Systems. We find the equipment highly versatile and easy to
  use. We have also been very impressed with the company's desire to continue to innovate and improve the hardware. In
  addition, we have been very happy with the after sales service and communication we have received from the company. I
  would strongly recommend Constant Systems to any research group who require cell disruption equipment."

                                    Further customer references available on request
About Constant   Systems

                   Constant Systems Ltd is a British company, part of the Score
                   Group PLC.
                   Providing niche bio-technical products and services to a
                   global client base, Constant Systems has been involved in the
                   design, development and manufacture of high-pressure cell
                   disruption equipment since 1989.
                   Constant Systems derives its name from providing a constant
                   and controlled cell disruptive environment for all machines
                   produced, giving our clients superior and constant results
                   whatever the application; hence the name "Constant Systems".
                   Machines installed worldwide in pharmaceutical and
                   biotechnology companies, universities, research institutes and
                   agencies, we are uniquely positioned to accommodate all your
                   cell disruption requirements and add significant value to your
                   scientific objectives and performance.
                   Constant Systems Head Office and facility is situated in
                   Daventry, England. Based in the Midlands (1 hr drive South of
                   Birmingham/1.5 hrs drive North of London), Constant Systems
                   Ltd is excellently positioned to accommodate all British and
                   International enquiries and service in the fastest possible time.
                   Our worldwide clients benefit from our global distribution
                   network located in North America, Europe, Middle East and
                   Asia. Please visit our website for the latest distribution
                   contact details.
                   The company mission evolves around our motto: To be the
                   finest resource of the highest quality cell disrupter products,
                   coupled with a customer orientated after market support
                   service which combined, allows all clients to produce superior
                   results.
                   Constant Systems is registered and approved to British
                   Standards Institute standard ISO 9001:2000.


                                                                            FS67550
Your Application and Cell                                           Success

 Constant Cell Disrupter Systems are extremely versatile and have been used for
 the following range of applications:

 Cell Disruption, Protein & DNA Extraction, Enzyme Release, DNA Manipulation
           Selective Breakage, Single Cell Isolation, Tissue Disruption
Individual organisms and samples processed by Constant Cell Disrupter Systems are too numerous to mention them
all, however the following lists examples:

Algae             Disruption of anabaena variables
Animal Cells      Selective breakage of chicken sperm
Bacteria          Good enzyme activity from recombinant e.coli. High protein recovery from mycobacterium smegmatis
Fungi             High specific activity of enzyme from Aspergillus nidulans.100% protein release from saccharomyces
(inc. yeasts)     cerevisiae. 99% Recombinant protein release from pichia pastoris
Mammalian        Disruption of cellular and intracellular plasma membranes from horse liver Isolation of viable single
Cells and tissue cells from calf spleen and lymph node

Parasites         Breakage of cytomegalovirus in nucleus of human cells
Plant Cells       Pectin extraction from banana fruit. Strawberry leaf DNA extraction
& Tissue
Viruses           Isolation of membrane antigens from toxoplasma gondii. Separation of nucleus and membrane in
                  amniotic cells
Environmental     Extraction of DNA from soil bacteria
Samples

E.coli Disruption & Guidance List                            Other Species
Constant Systems Cell Disrupters experience with             Please find below a list of some of the other more
E.coli is vast and breakage depends on the cell              common species of yeast with pressure vs. breakage:
culture conditions, length of growth cycle and
pressure utilised. We state the following as a guide to      •   Candida Albicans                  -   25   - 40 Kpsi
users for pressure compared to breakage with E.coli:         •   Candida Cloacae                   -   40   Kpsi
                                                             •   Candida Utilis                    -   15   - 40 Kpsi
• Release of DNA -                                           •   Hansenula Polymorpha              -   25   - 40 Kpsi
  Below 15,000 psi (1000 Bar)                                •   Kluyveromyces Fragilis            -   05   - 40 Kpsi
• Cytoplasmic Protein -                                      •   Pichia Augusta                    -   40   Kpsi
  15,000 psi - 20,000 psi (1350 Bar)                         •   Pichia Capsulatus                 -   40   Kpsi
                                                             •   Pichia Pastoris                   -   35   - 40 Kpsi
• Inclusion Bodies -                                         •   Pichia Picorna                    -   25   - 35 Kpsi
  20,000 psi - 27,000 psi (1900 Bar)                         •   Rhodotorula Glutinis              -   40   Kpsi
• Membrane Protein -                                         •   Saccharomyces Cerevisiae          -   27   - 35 Kpsi
  27,000 psi - 35,000 psi (2400 Bar)                         •   Schizosaccharomyces Pombe         -   15   - 40 Kpsi
Examples of High                                      Pressure Cell Disruption

 1. The effect of pressure on the cell disruption of                                    2. The effect of pressure on the cell
    Escherichia coli expressing soluble protein X                                          disruption of Saccharomyces Cerevisiae
    Completed at an independent and confidential customer                                  expressing protein X
    site, 2003.                                                                            Completed at an independent and confidential
 Preparation:
                                                                                           customer site, 2003.
 Cell paste (87g WCW) resuspended in 870ml 20mM TrisHCl/5 mM EDTA, pH                   Preparation:
 8.0. 40ml of the suspension was passed through Constant Cell Disruption                Cell pellet was resuspended in chilled 20mM Tris/1 mM EDTA,
 Systems (Z Plus Series) once with the following disruption pressures: 10, 17,          pH 7.98. The required lysis buffer was calculated as follows:
 20, 24, 27, 30, 33 and 35kPSI respectively. Samples were centrifuged at 8000           (46.82% w/v/20%w/v) x 233ml = 545ml. The resuspended
 rpm for 20 minutes at 4°C. Determined protein released with coomassie                  cells were mixed for 30 minutes at 6°C. pH and conductivity
 assay. Thereafter repeat passes of the same sample were passed through the             of resuspended paste; 6.98 at 12.2°C and 1.448mS/cm at
 cell disrupter to determine if multiple passes improved efficiency in protein          13.8°C. OD600 of resuspended cells; 76.
 yield between passes.
                                                                                        Method 1:
 Results 1 – Protein Release:                                                           40ML of the resuspended cells were passed through a
                                                   Pressure (kpsi)    Protein (ug/ml)   Constant Systems disrupter once at the following pressures; 8,
                                                          0                 414         10, 15, 20, 25 and 27kpsi.
                                                         10                3221
                                                         17                3838         Results 1:
                                                         20                4106         The efficiency of cell breakage was determined by the following
                                                         24                4162         method: Total Protein – 1ml of aliquots were centrifuged at
                                                         27                4261         13,000rpm for 30 minutes. The supernatant containing the
                                                         30                4247         soluble proteins was removed and total protein was
                                                         33                4240         determined in this fraction.
                                                         35                4236
                                                                                           Pressure       Protein mg/ml     Viable Cell Count – 1ml
                                                   Number of Passes   Protein ug/ml
                                                                                         Non Disrupted         0.88         of the cell lysate was
                                                          1               4261
                                                                                               8               2.20         added to 9ml of
                                                          2               4840
 For this particular strain of e.coli 27kpsi was                                              10               2.89         sterilised PBS (10 fold
                                                          3               4884
 the optimal pressure.                                                                        15               4.16         dilution). The following
                                                          4               4488
                                                                                              20               4.34         procedure was used to
                                                                                              25               4.41         carry      out     serial
 Results 2 – Percentage Disruption:                                                           27               4.61         dilutions:
 Colony Forming Units (cfu) per ml of sample
 OD600 1 = 0.4g/L dry cell weight                                                       100ul of each sample was spread onto agar plates.
 OD600 1 = 109 cfu/ml, therefore OD600 = 5.40 in 10L                                    Plates were incubated for 39 hrs at 22.5°C. Only those
 Resuspended cell paste in 1L = OD600 = 54 which gives 5.4 x 1010cfu/ml. Use            dilution factors that gave between 30 to 600 colonies
 this value to calculate percentage disruption at 27kpsi. Percentage cell               were counted and used in the calculation for
 disruption at 27kpsi = 99.98% disruption with 1 pass through the cell                  determining number of viable cells per ml. Colony
 disrupter. 99.98% breakage would leave 0.02% intact = 1.1 x cfu/ml 107                 forming units (cfu) of sample = average number of
                                                                                        colonies x 10/dilution factor.
 For the above experiment cell paste was suspended in 1:10w/v with lysis
 buffer. The experiment was repeated with a 1:30w/v dilution of cell paste with          Dilution 1:10   Sample Type and Volume    Volume of PBS (ml)
 lysis buffer.                                                                                10-1         1ml of neat sample              9
                                                                                              10-2            0.1ml of 10-1                9
 Sample                 10-2              10-4             10-6           CFU/ml
                                                                                              10-3            0.1ml of 10-2                9
 Non-disrupted          TNC               TNC              80             8.0 x 108
                                                                                              10-4            0.1ml of 10-3                9
 1 Pass                 TNC               688               6             6.9 x 107
                                                                                              10-5            0.1ml of 10-4                9
 2 Pass                 TNC                15               0             1.5 x 106
                                                                                              10-6            01.ml of 10-5                9
 TNC: Too numerous to count
 Fermentation 20: OD600 at harvest = 5.51/10L therefore 1L = 55, which gives            Percentage          cell         Sample        % Cell Disruption
 5.5 x 1010 cfu/ml in the non disrupted cells                                           disruption after 1 pass        Non Disrupted           0
                                                                                        through     the     cell             8               52%
 Cell Disruption following 1 pass through the cell disrupter = 99.87%.                  disrupter.                          10               68%
 99.87% breakage would leave 0.13% intact = 7.2 x 107 cfu/ml                                                                15               73%
                                                                                                                            20               87%
 Cell Disruption following 2 pass through cell disrupter = 99.997%.                                                         25               96%
 99.997% breakage would leave 0.003% intact = 1.7 x 106 cfu/ml.                                                             27              98.9%
Model Range
                 The One Shot model is an ideal research tool and is widely used throughout the
                 world for processing small samples in a variety of fields.
                 Process time per cycle (shot) is 25 seconds.
One Shot Model   Power Rating                                      Volume per Shot
                       0.75 KW        40 KPSI (2700 bar)    30 KPSI (2000 bar)   20 KPSI (1350 bar)   15 KPSI (1000 bar)
                                          Max Head              Max Head             Max Head             Max Head
                                           L
                     Quantity/shot          1-8ml             1.5-10.5ml             2-16ml               3-20ml

                 •     Incorporates our unique disruption head and precise hydraulic operating and
                       control system
                 •     Disrupter head mounted in stainless steel tray to avoid spillage
                 •     Easily dismantled for manual cleaning and autoclaving
                 •     Allows loading of solid sample, cell paste or suspended sample
                 •     Two One Shot cups are sold as standard and can be transferred to and from ice or
                       freezer for temperature control. Extra One Shot cups available on request
                 Dimensions (W,D,H): 480mm, 440mm, 450mm




                 The One Shot Head Adaptor incorporates the unique capability of the One
                 Shot model into the continuous flowing range of Cell Disrupters.
One Shot         Available on all continuous flow models for processing small, viscous or non-
                 flowing samples.
Head Adaptor                                                          Volume per Shot
                                                 40 KPSI (2700 bar)                        30 KPSI (2000 bar)

                     Quantity Shot                    1-10ml                                   1.5-13ml



                 The TS Series Benchtops are the ideal pilot models and are versatile enough to
                 adapt as your needs change.
TS Series        Power Rating                                           Flow Rates
Bench top                             40 KPSI (2700 bar)
                                          Max Head
                                                            30 KPSI (2000 bar)
                                                                Max Head
                                                                                 20 KPSI (1350 bar)
                                                                                     Max Head
                                                                                                      15 KPSI (1000 bar)
                                                                                                          Max Head

                     0.75KW              40ml/min              55ml/min             80ml/min            105ml/min
                     1.1KW               100ml/min            140ml/min            200ml/min            275ml/min

                 •     Incorporates our unique disruption head, precise hydraulic operating and control
                       system with an automatically supplied, continuous flowing Cell Disrupter
                 •     Touch screen control of disruption cycle automatic shut down when process complete
                 •     Cell Disrupter controls peristaltic process pump* for automatic supply of sample.
                       Process pump available on request
                 •     Disruption head mounted in stainless steel tray to avoid spillage
                 •     Partial containment and simple externally controlled CIP
                 •     Internal relief valve fitted for reverse flow of cleaning fluids
                 •     Various flow rates are available depending on desired maximum pressure and
                       power rating, alternative pressure heads available on request
1.1kW model
                 •     Pressure consistent and stable during disruption cycle
                 •     Full bio-containment inlet and outlet options available on request
                 Temperature Control: Disruption head is surrounded by unique cooling jacket (see
                 Principles of Operation). Bath circulator or chiller is recommended with a flow rate
                 of 1L/min (0.75kW), 1.5L/min (1.1KW). Bath circulator/chiller available on request
                 Dimensions (W,D,H):            700mm, 690mm, 750mm
                 * Not available on 0.75kW model
                 L
                   Customised “Low Loss” machine can be manufactured with a minimum volume of 0.1ml. Please enquire for details
The TS Series is the ideal pilot production model and is versatile enough to
adapt as your needs change.                                                                            TS Series
Power Rating
                   40 KPSI (2700 bar)
                                                    Flow Rates
                                        30 KPSI (2000 bar)   20 KPSI (1350 bar)   15 KPSI (1000 bar)
                                                                                                       Cabinet
                       Max Head             Max Head             Max Head             Max Head

    2.2KW            170ml/min            235ml/min            340ml/min            470ml/min
    4KW              400ml/min            550ml/min            800ml/min             1.1L/min

•    Incorporates our unique disruption head, precise hydraulic operating and control
     system with an automatically supplied, continuous flowing Cell Disrupter
•    Touch screen control of disruption cycle automatic shut down when process
     complete
•    Cell Disrupter controls peristaltic process pump for automatic supply of sample.
     Process pump available on request
•    Disruption head mounted in stainless steel tray to avoid spillage
•    Partial containment and simple externally controlled CIP
•    Internal relief valve fitted for reverse flow of cleaning fluids
•    Various flow rates are available depending on desired maximum pressure and
     power rating, alternative pressure heads available on request
•    Pressure consistent and stable during disruption cycle
•    Full bio-containment inlet and outlet options available on request
Temperature Control: Disruption head is surrounded by unique cooling jacket (see
Principles of Operation). Bath circulator or chiller is recommended with a flow rate
of 3L/min (2.2KW) & 6L/min (4KW). Bath circulator/chiller available on request
Dimensions (W,D,H):         700mm, 690mm, 1280mm



The High Area Inlet Valve Arrangement (HAIVA) was developed for processing large
samples or awkward samples such as filamentous cells.
It can be supplied as an alternative inlet arrangement on the TS Series 1.1KW and
2.2KW.
                                                                                                       HAIVA models
The inlet valve gives an inlet area some 5 times larger than standard. Flow rates,
temperature control and dimensions are the same as standard TS Series 1.1KW
and 2.2KW.




The 'B & C Series' are the ultimate industry cell disrupters, with the power and
processing ability to consistently disrupt the materials that you require.                             B & C Series
Power Rating                                        Flow Rates
                   40 KPSI (2700 bar)   30 KPSI (2000 bar)   20 KPSI (1350 bar)   15 KPSI (1000 bar)
                       Max Head             Max Head             Max Head             Max Head

    1.1KW            100ml/min            140ml/min            200ml/min            275ml/min
                                                                                                        Fully automated systems
    2.2KW            170ml/min            235ml/min            340ml/min            470ml/min           enabling CIP/SIP and
    4KW              400ml/min            550ml/min            800ml/min             1.1L/min           GMP manufacturing

•  Incorporates our unique disruption head, precise hydraulic operating and control
   system with an automatically supplied, continuous flowing Cell Disrupter with CIP
   and SIP capability
• Cell Disrupter controls peristaltic process pump for automatic supply of sample.
   Process pump available on request
• Fully contained and having internal ports and drain ports to allow CIP and SIP
• The 'B series' allows the CIP and SIP to be manually operated
• The 'C Series' includes all valves which can be tailored to customers
   requirements and validated
• IQ/OQ available on request
• Various flow rates depending on maximum pressure and power rating
• Pressure consistent and stable during disruption cycle
• Temperature control and dimensions as per TS Series
Validation: Industry standard validations available on request
 Model Range
                   In deciding which model is required for your specific requirements, you need to take into account the
                   sample viscosity, quantity, pressure, speed of processing, cleaning and containment.
SUPERIOR RESULTS


                        1. Sample Viscosity
                        Fluid                           All models
                        Fluid with soft particles       All models, but need to stir the sample in the reservoir for
                                                        continuous flow models
                        Fluid and viscous particles     One Shot Model, One Shot Head Adaptor or HAIVA
                        Viscous                         One Shot Model, One Shot Head Adaptor or HAIVA
                        Plant and tissue material       One Shot Model, One Shot Head Adaptor or mix with liquid and
                                                        use HAIVA

                        2. Quantity
                        Less than 50ml                  One Shot Model or One Shot Head Adaptor
                        More than 50ml                  Continuous Flow Models ideal.
                                                        One Shot Model or Adaptor can be used in batches

                        3. Maximum Pressure
                        Ideally the lowest temperature to achieve breakage is required. An approximate guide can be
                        offered:
                        up to 20kpsi                    Mammalian cells and tissue, plant cells, viruses
                        up to 30kpsi                    Algae, plant tissue, some yeasts
                        up to 40 kpsi                   Animal cells, bacteria, parasites, some yeasts

                        4. Flow Rate
                        Once 1-3 have been established flow rate can be worked out depending on how quickly samples
                        need to be processed.

                        5. Other Features
                        All models are quick and easy to use, contained during operation and simple to clean. It is easy to
                        set and adjust the pressure.

                        Moving through the range from One Shot to the C Series, you have the option of higher power to
                        increase flow rates, extra instrumentation for control and with the B and C Series clean-in-place
                        (CIP) and sterilise-in-place (SIP)



                     Candida Albicans                                          Candida Albicans Yeast Form Broken
                   Yeast Form Unbroken                                         with Constant Systems Cell Disrupter




                                                       ª
Principles of Operation

                Constant Cell Disruption Systems are based on the use of high
                pressure to force a sample through a small fixed orifice at high
                speed under controlled, contained and repeatable conditions.

                                                                                         Cooling in                      Cooling out




                1. As the high pressure
                   piston descends,
                   sample is introduced
                   into the high pressure
                   cylinder.




                                                                               2. The piston then forces the sample through the
                                                                                  jet at high speed. The rapid transfer of the
                                                                                  sample from a region of high pressure to one
                                                                                  of low pressure causes cell disruption


                                             3. When the jet containing disrupted cells hits the target, it
                                                is spread radially across the cooled heat exchange
                                                surface. Product then flows from the disruption chamber
                                                for collection. The hydraulics are recharged and the cycle
                                                continues.

                Reproducible Results
                Constant Cell Disruption Systems give reproducible and superior results because control
                of the disruption pressure is achieved by the hydraulic control system.
                                                                                   Disruption Return Recharging period
                The disruption pressure                                              stroke stroke for hydraulics
                cycle is shown adjacent.                                  30                                                         40
                This has been created by
                                                   Pressure (bar x 100)




                                                                                                                                          Pressure (psi x 1000)




                                                                                                                                     30
                analysing and recording                                   20
                the hydraulic pressure in                                                                                            20
                the operating cylinder,                                   10
                                                                                                                                     10
                which is related to the
                disruption pressure by the
                                                                                   2        4       6       8     10      12    14
                ratio of the piston areas.                                                          Time (second)
                                                  Graph showing a typical controlled pressure cycle in the cell disrupter
                Contained Process
                The disruption head, high pressure cylinder and cooling system have been
                designed with containment and safety in mind so that the volume of high pressure
                product is kept to a minimum at all times. The integrity of the containment system
                has been tested by the Biosafety Testing Station at the Centre for Applied
                Microbiology Research , UK. "…a high level of bio-containment." "No evidence of
                emission from the equipment in any test."
Model Specifications
                                       One Shot                            TS-Series
Power Rating                           0.75KW       0.75KW      1.1KW                  2.2KW        4KW
Maximum Pressure                       40Kpsi/      40Kpsi/    40Kpsi/                 40Kpsi/    40Kpsi/
Setting                                2700Bar      2700Bar    2700Bar                 2700Bar    2700Bar
Flow Rate (ml/min):                   0.1 – 20 ml   40 – 105   100 – 275               170-470   400 – 1,100
dependent on Maximum                    per shot     ml/min     ml/min                 ml/min      ml/min
Pressure Setting
Overall Size W, D, H (mm)              480mm,       700mm,     700mm,              700mm,         700mm,
                                       440mm,       690mm,     690mm,              645mm,         645mm,
                                       450mm        750mm      750mm               1,280mm        1,280mm
Weight (Kilos)                            85          110        130                    250         290
FEATURES & INSTRUMENTATION
Touch Screen Process                                   •           •                      •           •
Continuous Flow                                        •           •                      •           •
Cooling Jacket                             •           •           •                      •           •
Digital Pressure Adjustment/Control        •           •           •                      •           •
Secondary Pressure Containment             •           •           •                      •           •
Scalable to Higher Flow Rates              •           •           •                      •           •
Automated Control                                      •           •                      •           •
Outlet Temperature Indication                                      •                      •           •
Biological Containment*                    •           •           •                      •           •
PROCESS MONITORING
Disruption Finished                                    •           •                      •           •
Jet Blocked                                            •           •                      •           •
Pump Running                                                       •                      •           •
CIP Running
SIP Running
SERVICES REQUIRED
Cooling Fluid                                          •           •                      •           •
Steam
Air
Options
Trolley                                    •           •           •
One Shot Head Adapter                                  •           •                      •           •
Chiller/Cooler                                         •           •                      •           •
Service Contract                           •           •           •                      •           •
Process Pump                                                       •                      •           •
Alternative Pressure Heads                 •           •           •                      •           •
Inlet/Outlet Options                                   •           •                      •           •
CIP Pump
Automated CIP Station
Manual CIP Station
            B-Series                                                C-Series
 1.1KW      2.2KW         4KW         1.1KW                          2.2KW                          4KW
40Kpsi/     40Kpsi/     40Kpsi/      40Kpsi/                        40Kpsi/                      40Kpsi/
2700Bar     2700Bar     2700Bar      2700Bar                        2700Bar                      2700Bar
100 – 275   170-470    400 – 1,100   100 – 275                      170-470                    400 – 1,100
 ml/min     ml/min       ml/min       ml/min                        ml/min                       ml/min

700mm,      700mm,       700mm,      700mm,                         700mm,                       700mm,
690mm,      645mm,       645mm,      690mm,                         645mm,                       645mm,
750mm       1,280mm     1,280mm      750mm                         1,280mm                      1,280mm
  140         260         300          155                             275                          315


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                                             * For further detailed information please contact Constant Systems Limited
                                                                       Coventry
                                                                                       Rugby

                                                                                           Daventry
                           Liverpool

                  Birmingham



                               London




www.constantsystems.com

Global Distribution
            Network
             Australia
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                                       UK HEAD QUARTERS
               Canada                  Constant Systems Limited,
                 China                 Low March, Daventry, Northants,
       Czech Republic                  NN11 4SD, United Kingdom
              Denmark
                                       Tel: +44 (0)1327 314146 Fax: +44 (0)1327 314147
                France
                                       E-mail: constant@score-group.com Website: www.constantsystems.com
              Germany
              Hungar y
                  India
                                                     Registered Office in England No. 2433639
                   Italy                   FS67550
                 Japan
                 Korea
          Netherlands
          New Zealand
                                       US HEAD QUARTERS
               Poland                  Constant Systems Incorporated
              Portugal                 1900 Tramway Road                                                   Your local Representative:
            Singapore                  Sanford, NC 27330 USA
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                 Spain                 Tel: (1) 919 775 2531 Fax: (1) 919 776 0654
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For the latest distributor contact details please visit our website: www.constantsystems.com

				
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