Latest Molecular Technology Used in Poultry Breeding
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Latest Molecular Technology Used in Poultry Breeding document sample
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WINTER, 1998-99
IN THIS ISSUE:
Hy-Line Uses Advanced
New Molecular
Biology Laboratory Hard
Technology in the Breeding of
at Work — Pg. 8
Layers for the Next Millenium
Hy-Line has always been at the forefront contacted Dr. Elwood Briles, who had devel-
among poultry breeders in making use of the oped the method, and arranged for the tech-
Seminar Held for latest advances in technology. This began nique to be introduced to scientists at
Coastline Chicks in
Canada — Pg. 3 with the initiation of our company, when we Hy-Line. In the following years cooperative
were the very first to breed for hybrid perfor- work between Hy-Line and Dr. Briles demon-
Anniversaries Around the
World — Pg. 4 mance in chickens and continues today, 62 strated that the B blood group was associated
Hy-Line Seminars in
years later. with disease resistance. Pioneering studies
Argentina — Pg. 6 Hy-Line’s former parent company intro- from this work were subsequently published,
Hy-Line Holds China duced commercial hybrid seed corn, and including the landmark publication of the
Meeting — Pg. 9 revolutionized that industry. The geneticists association between the B21 blood type and
Italian Distributor who instituted Hy-Line applied the principles Marek’s disease resistance, which Hy-Line
Flourishes with Hy-Line of inbreeding and hybridization to Continued on page 2…
Layers — Pg. 10
chickens and in turn revolutionized
Hy-Line Distributor in the poultry industry. Before Hy-Line
Spain Builds New
Hatchery — Pg. 12 came on the scene, all chickens, both
egg and meat type, and all other
poultry for that matter were
produced and sold as pure lines.
Hy-Line introduced the first strain
9.1 Million cross chickens in the early 1940’s.
Pullets Hatched in June Within ten years, virtually all
Sets New Record — Pg. 7
commercial layers were hybrids.
Hy-Line North American
Production Managers
Subsequently Hy-Line moved away
Meet — Pg. 11 from inbreeding, but has continued
to refine the methods for the
improvement of the hybrid chicken.
In 1950, H. B. Wallace, Hy-Line’s
first president, learned of the recent
development of blood typing in “Puncture Score” device used exclusively by Hy-Line to measure
chickens at Texas A&M. H. B. eggshell strength.
eggshell quality. Through the years,
we have continually searched for
better and better methods to iden-
tify the breeding stock with the best
genetic eggshell strength. Out of
Hy-Line this research, Hy-Line developed
scientists use the “bomb score” device, which
tomorrow’s technology was used successfully for several
for tomorrow’s layer. years to improve shell strength.
Later Hy-Line developed the
“puncture score” device, which
proved to be quick, accurate, and
highly predictive of resistance to
breakage in the field. Hy-Line has
continued to use the basic tech-
nique of puncture strength assess-
ment for almost 30 years. However,
Hy-Line continues refining the
Hy-Line’s Molecular Biology lab technician using the powerful
method. We have recently auto-
tools of modern genetic science. mated this measurement by modi-
fying a high tech British device.
…continued from page 1
This has allowed us to perform the measure-
geneticists authored in the journal Poultry ment even quicker than in the past. The shell
Science in 1967. strength measurement is interfaced with a
We now know that the blood type gene computer, so that data is instantaneously
was actually serving as a marker for other recorded in a completely accurate manner.
genes that were tightly associated with it in Other egg quality measurements also
the same linkage group (chromosome 16). utilize the latest technology. For instance,
Blood types were serving as a “marker” for shell color is assessed by use of the Chroma
the genes we needed to improve livability. Meter. This reports three aspects of the
Hy-Line geneticists have now used blood appearance of the shell, reflectance, chro-
types to aid in the selection for disease resis- maticity on a red/green scale, and chromatic-
tance for over forty years. Once we purified ity on a blue/yellow scale. These measures
our populations for the best blood types, we are combined into a single index, designed to
found that we could use the differences in select for a deep brown shell color.
blood types between lines as a check to make Selection decisions have always been
sure that our lines stayed pure. We now have made with the assistance of the best available
quick tests for blood types that we use in our statistical tools. We are now utilizing method-
grandparent and great grandparent flocks to ology developed in recent years which makes
make sure that there is no mix-up. Blood use of all available data from ancestral and
typing therefore serves as both a selection tool collateral relatives. The data is submitted to
and a quality control tool. statistical manipulation to produce the Best
Today, Hy-Line’s Molecular Biology labo- Linear Unbiased Prediction (BLUP) of the
ratory is using the powerful tools of modern genetic merit of each potential breeding hen
genetic science to capitalize on the hundreds or rooster. This requires hours of time on a
of markers now available. The technique of high-speed computer for each line selected.
using markers to select for linked genes with The BLUP values are then brought together
desirable effects on performance is called into an index combining all traits of interest
Marker Assisted Selection (MAS). We were in with the proper weight, so that we can deter-
reality the first poultry breeder to utilize mine which are the best birds to use in
MAS, via the blood type gene. MAS undoubt- producing our grandparent and the next
edly will be even more valuable to us in the pedigreed generation.
future, because of the sheer numbers of The production data, which is used to
markers now available. build the statistical indices, is collected using
2 Another area in which Hy-Line has
pioneered with new technology concerns
bar code readers. Each night this data is
Continued on page 3…
…continued from page 2 test units is faxed each evening to the
uploaded into the main computer and is Research Center in Dallas Center, Iowa. Once
available the next day for use in making selec- there, it is updated each day, so that perfor-
tion decisions. We also use bar codes on the mance in these field tests can be closely moni-
eggs that are sent in to our egg quality labora- tored.
tory. This assures quick and accurate assign- Hy-Line will continue to make appropri-
ment of the egg quality data to the proper ate use of the latest in modern technology to
hen. supplement our established and long proven
Data that is collected from Hy-Line’s field program of genetic improvement.
Hy-Line’s state-of-the-art Biotechnology Center in Dallas Center, Iowa.
Seminar Held for Coastline
Chicks In Canada
A seminar was held October 26, in Region manager. Coastline Chicks is owned
Abbotsford, British Columbia, Canada for the by Mr. Ernie Unger, president and general
customers of the Hy-Line distributor, manager, and his family. Canadians
Coastline Chicks. Eighty- host seminar.
three producers and
allied industry people
were in attendance.
Speakers for the daylong
event included Hy-Line’s
personnel, Dr. Dennis
Casey, president; Steve
Rieger, vice president/
North America
distribution director; Dr.
Woodie Williams,
nutritionist; and Dr.
Doug Grieve, staff
veterinarian. The master
of ceremonies was Pete
Block, Hy-Line’s Western
Panel discussion during the event in British Columbia.
3
Anniversaries Around the World
Hy-Line International has more than 65 exclusive distributors around the world selling
Hy-Line products in over 110 countries. To honor our distributors, on certain anniversaries
Hy-Line awards a “Years of Service” plaque. Here are the latest:
International
distributors recognized.
Saudi Arabia
Presenting the 15 years of service
plaque to Mr. Saad Al Sabbar.
Al-Sabbar Farms is the sole distri-
butor for Saudi Arabia, importing
Hy-Line W-77 parent stock since
August 1983. Al-Sabbar Farms is
located in Al-Kharj, 140 kilometers
from Riyadh and is a leader in the
Saudi layer market.
(L - R) Dr.Ali, Dr. Chuck Howe, Saad Al-Sabbar, Miguel Paula, Eng.
Ibrahim Mustafa in Saudi Arabia.
Pakistan
Miguel Paula and Dr. Mohammad
Amjad presenting the 10 years of
service plaque to Mr. Tahir Zaidi,
from Conimpex Breedeing Farm.
Conimpex, based in Multan, Punjab,
imports Hy-Line W-77 breeders since
June 1988.
(L-R) Farjard Zaidi,Tahir Zaidi, Miguel Paula, Dr. Mohammad Amjad in
Pakistan.
Pakistan
Miguel Paula and Dr. Mohammad
Amjad presenting the 10 years of
service plaque to Mr. Mohammad
Patel, from Sunny Poultry Breeders.
Sunny Breeders, located in the city
of Karachi, Pakistan has imported
Hy-Line W-77 breeders since July
1988.
(L-R) Dr. Mohammad Amjad, Mohammad Patel, Miguel Paula.
4
Bangladesh
Mr. Mohammed Ali and Mr.
Sirajul Haque are partners in Dhaka
Hatchery located in Dhaka,
Bangladesh. Dhaka Hatchery
imported its first Hy-Line PS flock in Distributors
January 1993 and has been growing honored for years of
steadily since then, being today one of service.
the largest layer hatcheries in
Bangladesh.
(L-R) Sirajul Haque, Miguel Paula, Dr. Doug Grieve, Mohammed Ali
shown in Dhaka, Bangladesh.
Pakistan
Dr. Dennis Casey presenting the
10 years of service plaque to Dr.
Mohammad Amjad and Dr. Sarosh
Acram from Quality Breeders, based
in Rawalpindi. Dr. Amjad has had a
business relationship with Hy-Line
International since 1983, when he was
an employee of the previous Hy-Line
distributor in Pakistan. Hy-Line layers
are number one in that country.
(L-R) Dennis Dixon, Dr. M.Amjad, Dr. Dennis Casey, Dr. Sarosh
Acram, Miguel Paula.
Colombia
Mr. Fabio Dominguez, general
manager of Avicola Colombiana
receiving a 10 years of service award
from Dr. Dennis Casey. Avicola
Colombiana recently inaugurated its
new ultra modern hatchery and
breeder farm dedicated exclusively to
Hy-Line day-old chick production
that allows the company to offer the
Colombian industry with world-class
quality chicks. Avicola Colombiana
distributes Hy-Line W-36 and
Hy-Line Brown layers throughout
(L-R) Dr. Fabio Dominguez, Director of Avicola Colombiana
that important South American
country.
receives his award from Dr. Dennis Casey.
5
Hy-Line Seminars in Argentina
Hy-Line president of Cabaña Avicola Feller, and by
International Hy-Line officials.
and the Cabaña Avicola Feller, which has been in
Argentine the chick distribution business since 1958,
chick was founded 40 years ago in the city of
distribution Crespo some 500 Km north of Buenos Aires.
company, The company is one of the largest layer chick
Cabaña Avicola production companies in the country and
Feller S.R.L. will supply Hy-Line products on an exclusive
announced basis to customers throughout the South
their new American nation. Cabaña Avicola Feller has a
national hatchery in the Crespo area and four breeder
alliance at a farm centers located on 150 hectares.
series of kick off
Argentina, which is one of the largest
meetings held in Argentina during October.
grain and beef producing nations on earth,
The meetings, which were held in Buenos has 22,000,000 layers in production nationally.
New Latin Aires and Crespo, in the province of Entre The market is divided 55% white egg
American distributor Rios, where the headquarters and production placements and 45% brown eggers.
launched. center of the company are located, drew
Besides chick distribution Cabaña Avicola
about 200 customers, officials, journalists and
Feller also maintains commercial layers,
other dignitaries. Talks announcing the
produces started pullets and is a partner in
alliance were given by Hector Motta,
Technovo S.A., an egg breaking company.
(L-R) Dr.Augusto Motta, Hector Motta, Dennis Dixon, Juan Carlos Motta, and Thomas Dixon are shown at
Cabaña Avicola Feller headquarters in Argentina.
6
9.1 Million Pullets Hatched in
June Sets New U.S. Record
As Hy-Line’s fiscal year came to a close, June toward achieving such a goal, Hy-Line
emerged as the largest single hatch month in decided to take a more “in depth” look at the
the history of the Hy-Line hatchery business activity that surrounds such an Dometic sales
distribution system. In recognition of achievement. set new record.
everyone’s hard work and commitment
North America Business Activity
Facts for June 1998
9.1 Million Chicks Produced
s 2.4 eggs per pullet - 21.6 million eggs produced.
s 720,000 eggs per day (7 days per week) gathered, sorted,
trayed or cased by approximately 50 producers.
s 982,000 eggs a day (5-day week) that hatcheries picked up,
people processed and sat into incubators.
• 2,727 - 30 dozen cases per day
• 60,000 - 30 dozen cases per month (22-day month)
• 75 semi loads at 800 cases per load
s Actually hatched and sexed 18,600,000 straight run at 49%
females.
s On 18 hatch days per month - 1,033,000 straight run being
sexed daily.
s Processing, sorting, boxing and delivering approximately
505,000 females per 8 hour day (4 hatches per week) or
63,200 females per hour.
s Average truckload for delivery - 65,000 females.
• Equal to 140 truck deliveries per month or 8 loads per day.
7
New Hy-Line Molecular Biology
Laboratory Hard at Work
Are you curious about
what happens at the new
molecular biology lab? Just
Molecular
Biology Laboratory
continue reading for your
develops new quick tour of the new lab, get
techniques. acquainted with the indi-
viduals in the lab and find out
what they really do.
The purpose of the
molecular biology lab is to
use some of the new
techniques being developed
in molecular biology to
enhance selection efficiency
and progress with Hy-Line
genetic stocks.
When the geneticists
select the best birds for
breeding to improve egg Molecular biology lab technicians explore new frontiers of poultry genetic
quality traits, feed efficiency, potential.
etc., they select birds based on
their daughters’ performance
course this is not something that they can
in commercial conditions and how well their
figure out over night, but it has made some
family is performing with the various traits
big steps in finding differences in DNA
that they measure. However, they must wait
between the different Hy-Line lines.
until the birds can go through a production
cycle before they can determine just who has To get DNA from a bird, a very small
the best production. That can take several drop of blood is needed. Then they extract the
months of testing. Just imagine what would DNA from the blood cells. From one drop of
happen if they could check for the best birds blood they can get enough DNA to do 30,000
at hatch time. tests. So you can see that not very much DNA
for each test is needed. Over 600 of those very
How can the personnel of Hy-Line’s
small regions can be tested to look for
molecular lab do that? Those birds that have
differences. You can imagine that at the lab
the best traits also have the best genes. By
they are very busy. By the end of 1998 they
breeding from birds with the best traits they
will have done over 75,000 tests. The DNA
are increasing the number of birds that have
from each bird is tested, then compared to the
those really good genes. Genes are present in
DNA from different birds to see if they are the
the chicks when they hatch. So all they have
same. They are testing all of the
to do is look for the genes at hatch. That is
Hy-Line lines. After they are done, we will
where the molecular biology lab comes in!
have “fingerprinted” all the Hy-Line genetic
People at the lab use a procedure called stocks. This information can be used to check
PCR (that stands for Polymerase Chain flock purity and genetic identity.
Reaction). This procedure allows them to
Who are the people that work in the
actually see very small regions of the DNA of
molecular biology lab?
individual birds. DNA is what the genes are
actually made from. They look for differences Karol Field, technician, started in April of
in the DNA between birds. Then they see if 1998. He has a B.S. in biology (1996) from
birds with one form of the DNA are better at Graceland College. He grew up in Woodward,
8 laying eggs than birds with another form. Of Iowa and graduated from Woodward/
Granger High School in 1991. Karol lives Janet Fulton, molecular biologist. Started
in Dallas Center, Iowa. Before coming to in January of 1996. Janet has a B.S. in Poultry
Hy-Line, he worked at Pioneer in Johnston. Science from the University of British
He is the one who sets up those 75,000 tests Columbia and a master’s degree from the
per year. He helps to isolate DNA, prepares University of Saskatoon, Saskatchewan in
all samples for testing and keeps very good Animal and Poultry Science. She worked as a
notes. Karol is usually found in the PCR technician for six years before beginning
preparation room. graduate studies. In her student days, Janet
Amy McCarron, technician, started in studied poultry from the ground up (literally).
December of 1997. She has a B.S. in biology Her past responsibilities included hatching,
and Spanish from Loras College (1992) in banding, and dubbing chicks, artificial
Dubuque, IA, and a master’s degree in insemination, cleaning cages, brooders and
biology from the University of Iowa in 1995. manure scooping. Janet has a Ph.D. from Iowa
Amy lives in Dallas Center with husband Dan State University (1989) in Immunobiology,
Jones. Amy worked at the soybean breeding and did post doctoral work at the University
department at Pioneer before coming to of Minnesota and the Avian Disease and
Hy-Line. (The genetic principles are the same Oncology lab (USDA) in East Lansing, MI.
for soybeans and chickens). Amy helps Karol Janet supervises the molecular biology lab,
to set up all of those tests. She also does the does computer analysis and summarizes data.
computer analysis for every one of those Janet lives in Clive, IA, with her husband Jeff
75,000 tests. You will generally find Amy in Daly and three children, Cameron (13), Jocelyn
front of her computer in the lab. (11) and Ramona (7).
Hy-Line Holds China Meeting
Hy-Line International held its annual Although there are no official government
Chinese distributors’ meeting in Beijing figures, it has been estimated that the Chinese
recently with nearly 40 customers and indus- national layer population approaches one Second
try officials from all parts of the country in billion birds. The market is about 65% brown annual China meeting
attendance. eggs and 35% white, according to Zheng draws a crowd.
The national meeting, which was held in Chunning, Hy-Line’s China manager who
the Hy-Line headquarter office in the Chinese resides in that country.
capital city, was attended by representatives Hy-Line supplies both grandparent and
of 11 distributor organizations, government parent stock birds to the Chinese industry
officials and journalists. Featured speakers from its production center in the United
included Dr. Dennis Casey, Hy-Line’s presi- States. Ken Severson is responsible for sales
dent and Dr. Kenton Kreager, the company’s and service to the area.
technical service direc-
tor. Awards were
presented to long time
distributors and the
meeting concluded with
dinner at one of
Beijing’s oldest restau-
rants.
Hy-Line white and
brown egg products
have been sold in the
huge Chinese market
for nearly 15 years and
today Hy-Line is the
largest selling foreign
breed in the industry. Members of Hy-Line’s distribution network gathered recently at the Hy-Line
office in Beijing, China.
9
Italian Distributor Flourishes
with Hy-Line Layers
Technologically advanced, excellent ventilation systems. The collection of eggs is
quality control, and marketing aggressive- done by a fully automated system of nests.
ness are the strong characteristics of Azienda The farm uses individual staff for each house
Agricola Valversa the Hy-Line distributor for and the bird density is very low. The total
Italy. capacity of breeder hens is 150,000.
Italian
distributor advances in The Costa family, brothers Attilio, Paolo The company owns their own feed mill in
the market. and Valerio, own and operate the business order to have better control of the raw
supplying their customers with selected materials and formulations used in their
commercial Hy-Line layers from their rations.
Cocconato D’Asti headquarters and produc-
Hatchery Plant
tion center in the northwestern part of the
country. The hatchery plant has a total capacity of
more than 2,800,000 eggs. This translates into
Growing and Breeder Farms production of more than 300,000 female
When Valversa receives day-old parent chicks per week or about 16 million per year.
stock from Hy-Line in the U.S.A, they go The hatchery plant has the latest equipment
through careful monitoring done at the three in the Italian industry. It is fully computer-
different rearing units which have a capacity ized to control the different processes the eggs
of growing a total of 70,000 chicks. The and chicks go through. It has filtered air
breeder farm consists of five layer houses pressure positive, and special care is used in
located in different sites. Three of these cleaning and disinfecting everything in the
breeder houses have been totally modern- plant by a highly specialized staff in order to
ized. The Costa brothers have done a great comply with health regulations.
deal of improvements to their farms by Dr. Umberto Barale is responsible for the
adding ultra-modern equipment and using biosecurity and is assisted by joint owner
the latest technology. Valversa’s chicken Valerio Costa and his niece Dr. Valeria Costa.
houses are environmentally controlled using
state of the art feeding, drinking and Continued on page 11…
Agricola Valversa, Hy-Line distributor for Italy, has breeder growing facilities for 70,000 birds at its
10
production center.
…continued from page 10
Also, involved in the health and
quality of the chicks, as technical
support to customers is Dr. Paolo
Fiorini. The company pays close
attention to all aspects of biosecurity
particularly to bacteriological
control of Salmonella in the parents.
Eggs and chicks go through a
rigorous testing and samples are
sent to a government laboratory in
Torino where blood testing is also
done.
The production manager is
Maurizio Zonari. The sales and Italian distributor,Agricola Valversa, has state-of-the-art facilities
marketing staff is supervised by producing top quality chicks.
joint owner Paolo Costa and Gianni
Minardi and supported by Stefano
This family owned business has been the
Costa. The administrative staff composed of
Hy-Line distributor in Italy since 1979,
joint owner Attilio Costa and his daughter Dr.
marketing the Hy-Line Brown and W-36
Ilaria Costa.
layers.
Hy-Line’s North American
Production Managers Meet
A North America distribution
meeting was held September 16 and
17 at the Marriott Hotel in West Des Dometic
Moines, Iowa. managers hold
The meeting was attended by meeting.
Hy-Line’s regional managers, location
managers, staff members and guest
speakers, Dr. Stan Klevin from the
University of Georgia and Mr. Bob
Krouse, CEO of Midwest Poultry Inc.
in Mentone, Indiana. A full agenda of
topics was presented and discussed.
This included fiscal year end results,
breeder placement review and
performance, disease challenges and
biosecurity procedures as well as an
overview of food safety and HACCP
(Hazard Analysis Critical Control Dr. Klevin from the University of Georgia addressing the group of
Points). Hy-Line North American regional managers.
Praise and appreciation was
extended to the participants for their hard hatched and delivered 92.5 million-day-old
work and dedication in contributing to a very pullet chicks during the fiscal year. This was a
successful fiscal year 97/98. Hy-Line’s eight “record breaking” 14.5-million bird increase
North America commercial hatcheries over the previous year.
11
Hy-Line Distributor in Spain
Builds New Hatchery
pressure level which decreases as the
chicks move through the incubation,
New advances hatching and preparation process.
in Spanish industry. Once the chicks are processed they
are kept in a temperature and
humidity controlled holding room
until they are transported through-out
the Iberian Peninsula by specially
equipped chick delivery trucks. Ibertec
also sells products into North Africa.
The new hatchery is located on a
10,000 square meters of land. The main
hatchery building measures 72X30
meters.
At the same time Ibertec has built a
new poultry diagnostic laboratory
manned by three highly trained
veterinarians. It is the only one of its
kind in the Castilla and Leon area of
The interior of Ibertec’s new hatchery in Spain shows the new Spain.
state-of-the-art equipment that makes the new facility one of
Ibertec, which was founded in
Europe’s finest. 1994, maintains 185,000 layer breeders,
produces 12,000,000 commercial chicks,
Ibertec S.A., a distributor of Hy-Line raises 2.5 million started pullets and sells
W-77 white egg layer chicks in Spain, has 2,000,000 fertile eggs per year.
invested in a new hatchery near the city of
Valladolid 200 kilometers north of Madrid.
According to General
Manager Saturnino del
Canto the new plant was
inaugurated in July and is
one of the most modern in
Europe.
The Spanish hatchery
has 36 incubators with
57,600 egg capacity each and
18 hatchers. The state of the
art plant is divided into three
zones with the flow of chicks
and eggs always moving in
the same direction from the
areas of strictest biosecurity to Ibertec delivery trucks carry Hy-Line’s W-77 chicks to customers throughout
less restricted zones. The air in
the Iberian Peninsula.
each area has its own positive
Hy-Line International
West Des Moines, Iowa
U.S.A.
Fax: 515-225-6425
Printed in the U.S.A.
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