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Wheaton Cell Culture - Cell Culture

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Wheaton Cell Culture - Cell Culture Powered By Docstoc
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     Wheaton Science Products supplies tools to prepare            Wheaton Science Products offers large scale cell
     single cells from tissues to large-scale production of        production systems for the culture of mammalian or
     cells in stirred vessels.                                     insect cells. Maximum control of pH, dissolved oxygen,        In culture, anchorage dependent cells want to be
                                                                   temperature, agitation and antifoam is provided by            attached to a surface wall of a culture vessel or
     Typically, cellular tissue is derived from sources such       these bench-top systems. Unlike common tissue                 microcarrier beads suspended in culture media.
     as an egg, embryo, animal organ such as a spleen, or          culture flasks, which cannot provide total environmen-         Motionless methods of monolayer cell culture usually
     plant tissue. Wheaton tissue grinders (pages 43-46)           tal control, Wheaton cell production systems maximize         consist of T flasks which lay flat. Cells will grow on the
     reduce excised tissue to primary cells, as well as            conditions on the bench without the need for an               inside flask surface only where the media covers them.
     prepare subcellular components from harvested cell            incubator.                                                    Since adherent cells usually demonstrate contact
     packs. The goal is to create and establish primary cell                                                                     inhibition, cell growth is
     lines which can be used for production and/or                 The Cell Optimizer System® (page 54) is a small               restricted to the amount of
     ultimately become a master and/or working cell bank.          volume cell culture reactor which incorporates                contact surface area of the
     Wheaton Tissue grinders are also used to recover              sophisticated control technologies typically found in         flask. This severely limits the
     proteins, DNA and RNA for blots and other procedures.         large production culture systems. The Cell Optimizer          static method being used for
                                                                   System allows determination of cell growth and                large scale production of cells.
     Wheaton trypsinizing flasks (page 50) are used to              optimum production conditions prior to large scale
     further reduce tissue to cellular components. Place           culture. Operating parameters can be fine-tuned                As an alternative to T flasks,
     tissue in flask, add trypsin and stir the vessel using         rapidly in several vessels when working at a scale from       Wheaton Science Products
     the Wheaton Micro-Stir® magnetic stirrer (page 53).           250 milliliters to 3 liters.                                  offers various roller apparatus
     The deep-fluted flask design adds turbulence; a dam                                                                           (pages 58-63) and options to
     below the sidearm retains un-trypsinized tissue when          The Overdrive™ culture system vessels hold up to 45           allow for maximum flexibility.
     decanting. Dissociated cells can then be collected.           liters and provide production at a lower cost (page 56).      This allows the entire surface
     Trypsinizing flasks are also useful when removing cells        This system is versatile for suspension or                    of a roller bottle to be used to
     from microcarrier beads.                                      microcarrier cultures and can be combined with                grow monolayer cells without
                                                                   controllers and probes.                                       filling the entire bottle with
     Single cells can then be used to start a seed culture in
                                                                                                                                 expensive media. Using the
     a flask or multiple flasks. After reaching a specific            The Mini-Pilot Plant™ bioreactor provides total
                                                                                                                                 Wheaton Roller Apparatus,
     density, inoculate a suspension culture flask (pages           environmental control with 10,19 or 45 liter vessels
                                                                                                                                 increases surface area which
     51-52) or roller bottle (page 15) for high volume cell        operating on the bench-top. Incubators are eliminated
                                                                                                                                 equals larger cell yields, which
     production.                                                   with the innovative heating blanket. Probes, pumps,
                                                                                                                                 in turn, decrease production
                                                                   controllers and optional computer interface are
                                                                                                                                 costs.
                                                                   available. Large scale cell production is afforded at a
                                                                   fraction of the cost of comparable bioreactor systems.




                                     Wheaton Tissue Grinders enable primary cells
                                     to be prepared from excised tissues, as well
                                     as subcellular components from harvested cell
                                     packs. Tissue grinders are used to extract DNA,
                                     RNA and proteins for blots or other procedures.
                                     They are also used to enhance recovery of other
                                     cell products. Capacities on the grinders range
                                     from 0.1 mL to 55 mL. There are a variety of
                                     grinders to suit many tissue types.
                                     Tapered Tissue Grinders are ideal for connective tissue
                                     including heart, muscle and lung. These glass-on-glass
                                     grinders feature a long taper design that maximizes
                                     grinding efficiency.                                         •   Ideal for soft tissue
                                     Potter-Elvehjem Tissue Grinders incorporates a              •   Provided with loose and tight pestle
                                     serrated pestle design to disperse homogenate into the      •   Wheaton 33 low extractable borosilicate glass
                                     mortar cylinder. Standard units with Teflon® pestles are     •   Autoclavable
                                     well suited for soft tissue, such as brain and liver.       This tissue grinder is designed to retain a high percentage of cell nuclei and
                                     Tenbroeck Tissue Grinders have a hollow handle that         mitochondria in soft tissues or from cell cultures. In operation, the pestle ball is
                                     allows packing with ice, making them suitable for           encircled in liquid, which avoids heat buildup by reducing friction. This grinder
                                     tissue such as liver, intestines and heart.                 is ideal for enzyme studies. The initial grinding is performed using the “loose”
                                     Dounce Tissue Grinders are supplied with two types of       pestle. The grinding process is completed using the “tight” pestle. This grinder is
                                     pestles: a loose-fitting pestle for sample reduction and     used by moving the pestle up and down, and works best with cell suspensions or
                                     a second, tighter pestle for complete homogenization.       very soft tissue. The mortar has a large reservoir and pouring lip and is supplied
                                     The ball-shaped pestles leave cell nuclei intact in soft    with a “loose” pestle and a “tight” pestle. Two complete sets per case.
                                     tissues.
                                                                                                 Tight Pestle “A” Specification: 0.0010-0.0030 in (0.025 - 0.076 mm)
                                     The Wheaton Micro Tissue Grinder Kit offers a               Loose Pestle “B” Specification: 0.0035-0.0055 in (0.089 - 0.14 mm)
                                     complete selection of micro tissue grinders.
                                                                                                  Cat. No.      Size (mL)     Mortar, OD x Length (mm)     Overall Length (mm)   Qty/Case
                                     All Wheaton tissue grinders are manufactured from            357538           1                 11 x 48                        125                2
                                     Wheaton 33 low extractable borosilicate glass that
                                                                                                  357542           7                 13 x 82                        175                2
                                     conforms to USP Type I and ASTM E-438 Type I,
                                                                                                  357544           15                 22 x 94                       210                2
                                     Class A requirements and replacement parts can be
                                                                                                  357546           40                32 x 140                       285                2
                                     purchased separately.

                                WHEATON         1.800.225.1437 (U.S. & Canada only) | +1.856.825.1100 | +1.856.825.1368 (F) | www.wheatonsci.com
                                                                                                                                                                               44




                                                                                         Cat. No.                Description                       Qty/Case
                                                                                         358028                  2 mL Mortar Only                          2
                                                                                         358033                  5 mL Mortar Only                          2
                                                                                         358038                  10 mL Mortar Only                         2
                                                                                         358043                  15 mL Mortar Only                         2
                                                                                         358048                  30 mL Mortar Only                         2
                                                                                         358053                  55 mL Mortar Only                         2
                                                                                         358026                  2 mL Pestle Only                          2
                                                                                         358031                  5 mL Pestle Only                          2
                                                                                         358036                  10 mL Pestle Only                         2
•   Rugged alternative to glass Dounce Tissue Grinder                                    358041                  15 mL Pestle Only                         2
•   Precision machined from No. 316 stainless steel                                      358046                  30 mL Pestle Only                         2
•   Mortar and pestle provided as a matched set                                          358051                  55 mL Pestle Only                         2
•   Not autoclavable
Wheaton Dura-Grind® Stainless Steel Dounce Tissue Grinders provide a rugged
alternative to glass Dounce tissue grinders. Smooth pestle action requires less
effort than glass tissue grinders. This unit is precision-machined of No. 316
stainless steel to exacting tolerance of .0005 in. (0.013mm). The mortar has a
flat bottom allowing it to stand upright. Single pestle and mortar are supplied as a
matched set; they are not autoclavable. Weight: 2 lb (0.9 kg)
Cat. No.      Size (mL)     Mortar, OD x Length (mm)    Overall Length (mm)   Qty/Case
357572           7                 35 x 114                    171                  1
357574           15                35 x 114                    171                  1
357576           40                44 x 114                    171                  1



                                                                                         •   Exterior plastic coated glass mortar provide greater safety
                                                                                         •   Ideal for tissue homogenates
                                                                                         •   Mortar made from Wheaton 33 low extractable borosilicate glass
                                                                                         •   PTFE pestle with stainless steel shaft can be used with power homogenization
                                                                                         •   Autoclavable
                                                                                         Wheaton Safe-Grind plastic coated tissue grinders afford an added measure of
                                                                                         safety over uncoated glass tissue grinders. The heavy plastisol coating protects
                                                                                         the mortar from becoming scratched or checked, and assures you of a sure grip.
                                                                                         More importantly, the plastic coating creates a greater safety factor in case the
                                                                                         mortar cracks or breaks during power homogenization. The transparent coating
                                                                                         allows an unobstructed view of the homogenate during grinding. Wheaton Safe-
•   Ideal for tissue homogenates                                                         Grind tissue grinders are fully autoclavable. Grinding chamber clearance is .004”
•   Use with power homogenization                                                        to .006” (0.1 - 0.15 mm). Diameter of the stainless steel shaft is 0.25” (6.3 mm).
•   Parts interchangeable
                                                                                         Cat. No.    Size (mL)    Mortar, OD x Length (mm)    Overall Length (mm)   Qty/Case
•   Teflon® Pestle
•   Autoclavable                                                                         358003          2                11 x 45                    203                  2
                                                                                         358005          5                13 x 66                    219                  2
These grinders are precisely constructed with completely interchangeable parts           358007         10                16 x 74                    219                  2
to allow easier cleaning or replacement of damaged mortars. They are designed            358009         15                19 x 84                    219                  2
for use in preparation of tissue homogenates and other similar applications. The         358011         30               24 x 118                    266                  2
Teflon® pestle is for soft tissue such as brain or liver. The stainless steel rod is      358013         55               30 x 130                    266                  2
0.25” (6.3 mm) in diameter. The grinding chamber clearance is .004” to .006”
(0.1 to 0.15 mm). This unit is autoclavable and can be used for power
homogenization as with our overhead stirrer. However, we strongly recommend              Cat. No.                         Description                               Qty/Case
the use of safety-coated tissue grinders (see 358003 series) for added safety.           358004                           2 mL Mortar Only                                2
This unit is manufactured from Wheaton 33 low extractable borosilicate glass             358006                           5 mL Mortar Only                                2
that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.                 358008                           10 mL Mortar Only                               2
Cat. No.    Size (mL)     Mortar, OD x Length (mm)     Overall Length (mm)    Qty/Case   358010                           15 mL Mortar Only                               2
358029          2                 11 x 45                     203                   2    358012                           30 mL Mortar Only                               2
358034          5                 13 x 66                     219                   2    358014                           55 mL Mortar Only                               2
358039         10                 16 x 74                     219                   2    358026                           2 mL Pestle Only                                2
358044         15                 19 x 84                     219                   2    358031                           5 mL Pestle Only                                2
358049         30                24 x 118                     266                   2    358036                           10 mL Pestle Only                               2
358054         55                30 x 130                     266                   2    358041                           15 mL Pestle Only                               2
                                                                                         358046                           30 mL Pestle Only                               2
                                                                                         358051                           55 mL Pestle Only                               2



                                                                                               For a power homogenizer, see the Wheaton Overhead Stirrer on page 47.


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45


                                                                                                                         • Homogenizes connective tissue including heart,
                                                                                                                           muscle and lung
                                                                                                                         • Made from Wheaton 33 low extractable borosilicate
                                                                                                                           glass
                                                                                                                         • Interchangeable components takes the hassle out
                                                                                                                           of matching parts
                                                                                                                         • Autoclavable
                                                                                                                       These tapered tissue grinders have an improved
                                                                                                                       design, which incorporates a longer tapered surface
     •   Made from Wheaton 33 low extractable borosilicate glass                            on both the mortar and pestle. The conical surface allows initial size reduction
     •   Micro size for extremely precise work                                              followed by passage through the cylindrical section for the final homogenization
     •   Reservoir and pouring lip are incorporated into the design                         step. Grinding efficiency is improved, and less time is required as compared to
     •   Autoclavable                                                                       the Tenbroeck and Potter-Elvehjem designs. When the pestle is “bottomed” within
     Similar to the all-glass Potter-Elvehjem style, but micro size for extremely precise   the mortar, the clearance is a uniform .004” to .006” (0.1 to 0.15 mm).
     work, this unit is designed for delicate hand operation. Clearance between pestle      The components are interchangeably ground, which allows easier replacement
     and tube is .004” to .006” (0.1 to 0.15 mm). A reservoir and pouring lip are           of parts; there is also no need to keep parts matched during cleaning. The
     incorporated into the design. This grinder is made from Wheaton 33 low                 ground glass surface on both the mortar and pestle are capable of homogeniz-
     extractable borosilicate glass that conforms to USP Type I and ASTM E-438              ing connective tissue including heart, muscle and lung. This unit can also be
     Type I, Class A requirements.                                                          used for homogenizing skin and plant tissue, and is made from Wheaton 33 low
     Cat. No.    Size (mL)    Mortar, OD x Length (mm)    Overall Length (mm)   Qty/Case    extractable borosilicate glass that conforms to ASTM Type I, Class A and USP
     357844         0.1               4 x 65                     110                  2     Type I requirements.
                                                                                            Cat. No.    Size (mL)        Mortar, OD x Length (mm)    Overall Length (mm)   Qty/Case

     Cat. No.                     Description                                   Qty/Case    358103          1                    11 x 49                    130                  1
                                                                                            358107          3                    11 x 86                    220                  1
     357841        0.1              Pestle only                                       2
                                                                                            358111          5                    13 x 93                    220                  1
     357843        0.1              Mortar only                                       2
                                                                                            358115         15                   18 x 114                    240                  1


                                                                                            Cat. No.                        Description                                    Qty/Case
                                                                                            358102                          1 mL Mortar Only                                     2
                                                                                            358106                          3 mL Mortar Only                                     2
                                                                                            358110                          5 mL Mortar Only                                     2
                                                                                            358114                          15 mL Mortar Only                                    2
                                                                                            358101                          1 mL Pestle Only                                     2
                                                                                            358105                          3 mL Pestle Only                                     2
     with Radial Serrations                                                                 358109                          5 mL Pestle Only                                     2
                                                                                            358113                          15 mL Pestle Only                                    2
     •   Mortar made from Wheaton 33 low extractable borosilicate glass
     •   Radial serrated Teflon® pestle
     •   Interchangeable parts
     •   Autoclavable                                                                                               • Teflon pestle with stainless steel rod fits power
     This grinder has a serrated Teflon® pestle that disperses homogenate into the                                     homogenizer
     mortar cylinder more efficiently. These units are precisely constructed with                                    • Homogenizes soft tissue such as brain
     completely interchangeable parts, allowing for easier replacement of parts so you                              • Mortar made from Wheaton 33 low extractable
     don’t need to keep pieces matched during cleaning. Grinding chamber clearance                                    borosilicate glass
     is .004” to .006” (0.1 to 0.15 mm). The unit can be autoclaved. Depth of the ser-                              • Interchangeable components takes the hassle out of
     rations is .020” to .025”. Diameter of the stainless steel shaft is 0.25” (6.3 mm).                              matching parts
     Cat. No.    Size (mL)   Mortar, OD x Length (mm)     Overall Length (mm)   Qty/Case                            • Autoclavable
     357969          2               11 x 45                     203                  2                             This unit is the same as the Wheaton 358103 series, but
     357974          5               13 x 66                     219                  2                             with a Teflon® pestle for soft tissues such as brain. The
     357979         10               16 x 74                     219                  2                             stainless steel rod is 0.25” (6.3 mm) in diameter and is long
     357984         15               19 x 84                     219                  2                             enough to fit into the chuck of the Wheaton Overhead Stirrer.
     357989         30              24 x 118                     266                  2
     357994         55              30 x 130                     266                  2     Cat. No.    Size (mL)        Mortar, OD x Length (mm)   Overall Length (mm)    Qty/Case
                                                                                            358133          1                    11 x 49                   130                   1
     Cat. No.                     Description                                   Qty/Case    358137          3                    11 x 86                   220                   1
     358028                       2 mL Mortar Only                                    2     358141          5                    13 x 93                   220                   1
     358033                       5 mL Mortar Only                                    2     358145         15                   18 x 114                   240                   1
     358038                       10 mL Mortar Only                                   2
     358043                       15 mL Mortar Only                                   2     Cat. No.                                Description                            Qty/Case
     358048                       30 mL Mortar Only                                   2
                                                                                            358132                                  1 mL Mortar Only                             2
     358053                       55 mL Mortar Only                                   2
                                                                                            358136                                  3 mL Mortar Only                             2
     357966                       2 mL Serrated Pestle Only                           2
                                                                                            358140                                  5 mL Mortar Only                             2
     357971                       5 mL Serrated Pestle Only                           2
                                                                                            358144                                  15 mL Mortar Only                            2
     357976                       10 mL Serrated Pestle Only                          2
                                                                                            358131                                  1 mL Pestle Only                             2
     357981                       15 mL Serrated Pestle Only                          2
                                                                                            358135                                  3 mL Pestle Only                             2
     357986                       30 mL Serrated Pestle Only                          2
                                                                                            358139                                  5 mL Pestle Only                             2
     357991                       55 mL Serrated Pestle Only                          2
                                                                                            358143                                  15 mL Pestle Only                            2

                               WHEATON          1.800.225.1437 (U.S. & Canada only) | +1.856.825.1100 | +1.856.825.1368 (F) | www.wheatonsci.com
                                                                                                                                                                                   46




                                                                                       • Teflon® spacer aligns the pestle and reduces
                                                                                         aerosoling of the product
                                                                                       • Made from Wheaton 33 low extractable
                                                                                         borosilicate glass
                                                                                       • Autoclavable
                                                                                       This unit consists of a finely ground glass pestle and
                                                                                       tube. A Teflon® spacer aligns the pestle and reduces
•   Ideal for grinding liver, intestine and heart tissue                               aerosoling of the product. The mortar and pestle
•   Made from Wheaton 33 low extractable borosilicate glass                            come as a matched set. The pestle is manufactured
•   Hollow pestle can be packed with ice                                               from Wheaton 33 low extractable borosilicate glass
•   Pour lip on mortar                                                                 that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.
•   Autoclavable                                                                       Cat. No.     Size (mL)      Mortar, OD x Length (mm)       Overall Length (mm)   Qty/Case
All-glass Tenbroeck tissue grinders come with precision-made, interchangeable          357848         0.2                   7 x 33                         115                2
pestles and tubes; no need to keep components matched. This also allows for
easier replacement of damaged parts. The clearance between pestle and tube is
.0035-.0065” (0.09-0.16 mm). While this grinder is designed for hand operation,
it can be slow-speed motor-driven by inserting a rubber stopper (size 00) and
metal rod into pestle. The hollow handle permits packing with ice. This unit also
features an expanded reservoir and pouring lip. Designed for tissues such as                                                         • With Teflon® Pestle and Screw Cap
liver, intestines and heart, it’s made from Wheaton 33 low extractable borosilicate                                                  • Made from Wheaton 33 low
glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.                                                         extractable borosilicate glass
                                                                                                                                     • Autoclavable
Cat. No.    Size (mL)    Mortar, OD x Length (mm)     Overall Length (mm)   Qty/Case
357421          1                 11 x 48                    140                  2                                            This unit features a screw thread neck
357422          2                 11 x 50                    140                  2                                            for use with the Wheaton Connection®.
357424          7                 16 x 82                    190                  2    The 13-425 cap has an open top and a Teflon® faced silicone rubber liner. The
357426         15                 22 x 94                    250                  2    clearance between pestle and mortar is 0.002-0.006” (0.05-0.15 mm). These
357428         40                32 x 140                    320                  2    grinders are manufactured from Wheaton 33 low extractable borosilicate glass
                                                                                       that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.
                                                                                       Cat. No.     Size (mL)     Mortar, OD x Length (mm)    Overall Length (mm)       Qty/Case
                                                                                       357535         0.5                 13 x 37                     115                     2


                                                                                       Cat. No.                            Description                                  Qty/Case
                                                                                       357536                              Extra Mortar and Cap                              10
                                                                                       357537                              Extra Pestle                                       2




•   Made from Wheaton 33 low extractable borosilicate glass
•   Complete kit includes 7 micro tissue grinders
•   Replacement parts available separately
•   Autoclavable
A complete selection of Wheaton Micro Tissue Grinders is conveniently packed           •   Disposable tissue grinder eliminates cross contamination
in a lightweight protective carrying case. The 0.5 mL micro tissue grinder with        •   Useful for radioactive samples
screw cap can be used for tissue grinding as well as for additional                    •   Small grinder area allows for high sample recovery
procedures. This particular tissue grinder chamber can be safely joined with           •   Made from Wheaton 33 low extractable borosilicate glass
another threaded-glass component, if needed, using the Wheaton Connection®.            •   Autoclavable
The Micro Tissue Grinder Kit is packed in a high-density polyethylene case with
foam inserts.                                                                          The Disposable Micro Tissue Grinder allows for homogenized tissue micro
                                                                                       samples without cross-contamination. This unit is especially useful for radioactive
Cat. No.         Description                                    Qty/Kit     Qty/Case
                                                                                       samples. No washing is necessary; just dispose of the tissue grinder after use.
358204           Complete Kit                                      1              1    The small grinding area allows high sample recovery. This disposable grinder is
                                                                                       made from Wheaton 33 low extractable borosilicate glass that conforms to USP
                                                                                       Type I and ASTM E-438 Type I, Class A requirements.
357421           1 mL Tenbroeck Tissue Grinder                     1              2
357535           0.5 mL Tissue Grinder, G.P.I. 13-425 cap          1              2    Mortar Size: 10 x 44 mm
357538           1 mL Dounce Tissue Grinder                        1              2    Pestle Size: 7 mm sphere
357844           0.1 mL Tissue Grinder                             1              2    Cat. No.       Size (mL)       ID x Length (mm)        Overall Length (mm)       Qty/Case
357848           0.2 mL Tissue Grinder                             1              2    357860           0.75               8 x 44                     88                     25
358029           2 mL Potter-Elvehjem Tissue Grinder               1              2
358133           1 mL Tapered Tissue Grinder                       1              1

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47


                                                                                       Specifications:
                               • Allow for power homogenization or mixing
                               • Speed control capable of RPM/torque                   Speed Range:             300-5,000 RPM (Under load)
                                 combinations                                          Electrical Requirements: 120 VAC, 50/60 Hz, 50 watts
                               • Use only with tissue grinders with stainless steel    Dimensions:              4-1/2” x 5” x 12” (11.5 x 13 x 30 cm)
                                 rods                                                  Weight:                   6 lbs (2.7 kg)
                               • Keyless chuck accepts shaft size to 7.9 mm            Cat. No                Description                                                   Qty/Case
                               • One year warranty                                     903475                 Complete Unit                                                         1
                               The Wheaton Overhead Stirrer is a compact unit
                               with solid state speed control capable of providing
                               RPM/torque combinations for most laboratory opera-      Cat. No       Description                                                              Qty/Case
                               tions such as homogenizing or mixing. The Wheaton       905275        Swivel Blade Paddle for Stirring Liquids in Vessels with Side Entry Port
                               Overhead Stirrer should be attached only to tissue                    or for Vessels with Narrow Openings. Shaft 1/4” x 12” (6.3 x 305 mm).
                               grinders with Teflon® pestles and with stainless steel                 Blade Measures 1/2” x 2” (12.7 x 50.8 mm). Stainless Steel                      1
                               rods. Unit and motor are mounted in a housing with      905462        Extension Rod, 1/4” x 6” (6.3 x 153 mm).
                               epoxy finish and is provided with a 6” aluminum rod                    For Use with Extension Sleeve for Lengthening Paddles. Stainless Steel          1
                               and clamp for attachment to support stand. Keyless      905465        Extension Rod, 1/4” x 12” (6.3 x 305 mm).
                               chuck accepts shaft sizes to 5/16” (7.9 mm). (Sup-                    For Use with Extension Sleeve for Lengthening Paddles. Stainless Steel          1
                               port stand not included.) Unit comes with a one year    905475        Extension Sleeve for 1/4” (6.3 mm) Diameter Rods, with Set Screws.
                               warranty.                                                             For Use in Lengthening Paddles. Stainless Steel                                 1


                                                                                       liner used for sealing volatile samples has cross slits for easier needle
                                                                                       penetration. An optional liner is also available, Teflon-coated silicone septa,
                                                                                       which plugs into each of the 96 vials for better sealing capabilities. The
                                                                                       cover locks into place and has built-in O-rings, which provide a secondary
                                                                                       sealing system.
                                                                                       The Wheaton Multi-Tier Microtiter Plate is available assembled with the
                                                                                       base plate, vials, spacers, Teflon/Silicone slit liner and cover, or as indi-
                                                                                       vidual components.
                                                                                       Wheaton microplates provide the advantages of glass without the cost!

                                                                                       Specifications:
                                                                                       •   Standard 8 x 12 format with an alpha-numeric ID grid
                                                                                       •   Glass conical bottom vials made of Type I borosilicate glass
                                                                                       •   0.5 mL, 9 x 17 mm - fits in base plate
     Wheaton microplates extend the uses of traditional microplates. Their             •   1.0 mL, 9 x 30 mm - requires spacer and base plate
     unique combination of glass and plastic provide the benefits of each               •   1.5 mL, 9 x 44 mm - requires 2 spacers and base plate
     component while maintaining low cost. Wheaton microplate systems are
     useful for autosamplers and sample storage. The glass inserts are inert,          Advantages:
     and do not contain extractable materials. Wheaton microplates have                • Use the same base plate for all vials and sample sizes
     sufficiently large volume inserts that perform as mother plates for high           • Open-bottom base plate provides even heating or cooling of vials when
     throughput screening applications. The microplates can be used for small            immersed
     fraction collection, great for collecting fractions in a format compatible to     • Cover locks the liner in place and prevents vial pullout
     your auto injector, saving time and effort.                                       • Base plate does not float in water bath
                                                                                       • Conical design of glass vials permits maximum sample access
     Analytical chemistry laboratories use microtiter plates for different experi-
                                                                                       • Secure fit of vials in plate permits use with shakers or vortex mixers during
     mental assays, including sorbent assays, high throughput screening
                                                                                         sample preparation
     assays, and combinatorial chemistry analyses. Our microtiter plate can
                                                                                       • Teflon®/silicone liner with cross slits permits easier needle penetration
     have a fixed height or it can be multi-tiered for adjustable height to
                                                                                       • Glass vials provide inert solvent-resistant environment for samples
     support different-sized vials. The plate can be built via spacers, to hold
     capacities of 0.5 mL, 1.0 mL and 1.5 mL, depending on the height limita-
     tions of the instrument. The plate can hold various length 9 mm diameter
     conical bottom or flat bottom glass vials.
                                                                                                                                Plates manufactured using ABS (Acrylonitrile
     Many of the assays performed in the analytical chemistry laboratories                                                      Butadiene Styrene) have good chemical
     require heating or cooling of the vials to simulate in vitro conditions or for                                             resistance against acids and bases.
                                                                                                                                Includes base plate, vials, spacers, Teflon/
     temperature sensitive experiments. The heating or cooling is usually done
                                                                                                                                Silicone slit liner and cover, or as individual
     using liquids or gases. Our base plate has an open bottom so that the                                                      components.
     microtiter plate can be immersed in heating or cooling fluids allowing total
                                                                                       Cat. No.             Description                                                     Qty/Case
     fluid contact with the vials. This permits uniform transfer of temperature to
                                                                                       W845100              0.5 mL Microplate System - with Glass Conical Vials                     1
     and from the vials.                                                               W845110              1.0 mL Microplate System - with Glass Conical Vials                     1
     The cover and liners for the plate provide a seal for individual samples to       W845106              1.5 mL Microplate System - with Glass Conical Vials                     1
     prevent sample evaporation and cross contamination. The Teflon®/silicone

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                                                                                                                                                                             48


                                   Plates manufactured using Topas COC (Cyclic
                                   Olefin Copolymer) have excellent clarity and
                                   heat/chemical resistance. TOPAS COC Plates
                                   have excellent chemical resistance to aqueous
                                   acids and bases and to most polar solvents. The
                                   plates can be autoclaved and sterilized. Samples
                                   can be heated in Topas COC Plates. Includes
                                   base plate, vials, spacers, Teflon/Silicone slit
                                   liner and cover, or as individual components.
Cat. No.           Description                                            Qty/Case
W845102            0.5 mL Microplate System – with Glass Conical Vials          1
W845112            1.0 mL Microplate System – with Glass Conical Vials          1     Sealing system is designed for Wheaton’s microplates and is compatible with
W845108            1.5 mL Microplate System – with Glass Conical Vials          1     most 96-well plates with rounded well holes. Molded PTFE/Silicone has 96 plugs
W845101            0.5 mL Microplate System – with Flat Bottom Vials            1     that lock into the vials and create an inert environment for the samples while
W845113            1.0 mL Microplate System – with Flat Bottom Vials            1     preventing cross contamination. The molded liner is available prescored, which
W845107            1.5 mL Microplate System – with Flat Bottom Vials            1     can be easily pierced by an autoinjector needle. The liners are made of Teflon®
W845114            2.0 mL Microplate System – with Flat Bottom Vials            1     lined medical-grade silicone that conforms to USP Class VI requirements.
                                                                                      Cat. No.           Description                                              Qty/Case
                                                                                      W845124            Molded PTFE/Silicone Liner for Standard 96-Well Plates         5




The Wheaton Multi-Tier™ Microplate is available assembled with the base plate,
vials, spacers, Teflon®/Silicone slit liner and cover, or as individual components.
Cat. No.           Description                                            Qty/Case    Sealing system is designed for Wheaton’s microplates and is compatible with
ABS Sections                                                                          most 96-well plates with rounded well holes. Molded PTFE (polytetrafluoroeth-
W845120            Cover for Multi-Tier™ Microplate                             5
                                                                                      ylene)/Silicone 8-plug strips and individual plugs allow for limited testing in the
W845121            Spacer for Multi-Tier™ Microplate                            1
                                                                                      Multi-Tier Plate or can be used for specific sample identification. The strips and
W845122            Base Plate                                                   1
                                                                                      plugs are made of Teflon® lined medical-grade silicone that meets the
                                                                                      requirements of the USP Class VI.
Topas Sections
W845116            Cover for Multi-Tier™ Microplate                             5     Cat. No.           Description                                              Qty/Case
W845117            Spacer for Multi-Tier™ Microplate                            1     W845130            Molded PTFE/Silicone Strips - 8 Plugs                         12
W845118            Base Plate                                                   1     W845131            Prescored Molded PTFE/Silicone Strips - 8 Plugs               12
                                                                                      W845132            Molded PTFE/Silicone Plug Singles                            100
                                                                                      W845133            Prescored Molded PTFE/Silicone Plug Singles                  100
                                                                                      W845115            Liner PTFE/Butyl/PTFE, Flat, Pre-scored                        5
                                                                                      W845123            Liner PTFE/Silicone/PTFE, Flat, Pre-scored                     5
The glass conical vials are manufactured from Type I borosilicate glass. The glass    W845125            Molded Liner TFE/Butyl/TFE, 96 Plug                            5
provides an inert solvent resistant environment for samples. The vials fit securely    W845126            Molded Liner TFE/Silicone/TFE, 96 Position                     5
in our base plate.                                                                    W845127            Molded Liner TFE/Butyl/TFE, 96 Plug                            5




The vial loader is a fast, convenient choice for loading our vials into the base      Cat. No.           Description                                              Qty/Case
plate. Just peel away the packaging and place an empty base plate on top of the
                                                                                      W845119            Solid Clear Cover                                              5
vial loader. Invert the entire unit, and the plate is ready for use.
Cat. No.           Description                                            Qty/Case
W845140            0.5 mL Glass Conical Vials in Vial Loader                   96
W845141            1.0 mL Glass Conical Vials in Vial Loader                   96
W845142            1.5 mL Glass Conical Vials in Vial Loader                   96

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49




                                                • For use as slant or shake cultures
                                                • Autoclavable
                                           These standard round-bottom culture tubes
                                           are manufactured from 51 expansion
                                           borosilicate glass that conforms to ASTM
                                           Type I, Class B and USP Type I
                                           requirements. The tubes can be reused
                                           many times. The caps have a white 14B             • With Cleanout Port and Caps
     styrene-butadiene rubber liner or a Teflon® faced 14B styrene-butadiene rubber           • Autoclavable
     liner. See the Cap and Closure Section for replacement caps.
                                                                                             These flasks are similar to Catalog Number 351054, but they have a lower
     Cat. No.     Capacity (mL)             Dia x H (mm)              Cap Size   Qty/Case
                                                                                             sidearm, a side cleanout port, a 38-430 size top cap and a 20-415 size sidearm
     With White 14B Rubber Lined Cap (Autoclavable)                                          cap. The caps have white styrene-butadiene rubber liners.
     358606            11                    16 x 100                 15-415           144
                                                                                              Cat. No.           Capacity (mL)   Volume (mL)        Sidearm Size (mm)   Qty/Case
     358607            14                    16 x 125                 15-415           144
                                                                                              351454                 300            200                 14 x 140              1
     358610            27                    20 x 150                 18-415           144
                                                                                              351456                 300            200                 19 x 140              1
     With Teflon® Faced 14B Rubber Lined Caps (Autoclavable)                                   351486                 500            250                 19 x 140              1
     358646            11                    16 x 100                 15-415           144    351514                1000            600                 14 x 140              1
     358647            14                    16 x 125                 15-415           144    351516                1000            600                 19 x 140              1
     358650            27                    20 x 150                 18-415           144




                                   • Ideal for media storage and cell culture
                                   • Autoclavable
                                   This flask is designed for biological/media storage or
                                   culture. The flask is manufactured from Wheaton 33
                                   low extractable borosilicate glass that conforms to USP
                                   Type I and ASTM E-438 Type I, Class A
                                   requirements.


     Cat. No.               Capacity (mL)                  Cap Size              Qty/Case
     635134                     25                         20-400                        1
     635165                    1000                        38-430                        1    Three baffle flask                    Four baffle flask




                                                                                             • Maximizes aeration for improved growth
                                                                                             • Break resistant lip
                                                                                             • Autoclavable
                                                                                             Designed for use on either rotary or reciprocating shakers, these flasks are
                                                                                             specially designed to maximize aeration for improved growth. They feature a
                                                                                             break-resistant lip, which accommodates a cotton plug or a stopper, and a
                                                                                             marking spot on the side of flask. These flasks are manufactured from Wheaton
                                                                                             33 low extractable borosilicate glass that conforms to USP Type I and ASTM
                                                                                             E-438 Type I, Class A requirements. The four baffle flask features a dam at the
                                                                                             base of the neck that reduces splashing.
                                                                                              Cat. No.                           Capacity (mL)                          Qty/Case
     • For use with colorimeter or spectrophotometer                                          With Three Baffles
     • Scratch and striation free side tube                                                   353255                                  50                                      1
     • Autoclavable                                                                           353257                                 125                                      1
     These flasks allow growth and turbidity reading at selected intervals without             353259                                 250                                      1
     sampling. Simply tilt flask to allow filling of the sidearm and insert sidearm into        353262                                 500                                      1
     colorimeter or spectrophotometer. The side tube is scratch and striation free.           353264                                 1000                                     1
     Designed to operate on a platform shaker, the flask holds approximately 350               353266                                 2000                                     1
     mL to the base of the sidearm. Material cannot enter the sidearm when on the             With Four Baffles
     shaker. The break-resistant lip accommodates a cotton plug or stopper. These             354235                                  50                                      1
     flasks are manufactured from Wheaton 33 low extractable borosilicate glass that           354237                                 125                                      1
     conforms to USP Type I and ASTM E-438 Type I, Class A requirements.                      354239                                 250                                      1
     Cat. No.               Capacity (mL)            Sidearm Size (mm)           Qty/Case     354242                                 500                                      1
     351054                     500                        14 x 130                      1    354244                                 1000                                     1



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                                                                                                                                                                                       50



  Wheaton trypsinizing flasks are used to further reduce tissue to
  cellular components. Place tissue in flask, add trypsin and stir the
  vessel using the Wheaton Micro-Stir® magnetic stirrer. The deep-
  fluted flask design promotes mixing turbulence; a dam below the
  sidearm retains untrypsinized tissue when decanting. Dissociated
  cells can then be collected. Trypsinizing flasks are also useful when
  removing cells from microcarrier beads. Single cells can then be
  used to start a seed culture in a flask or multiple flasks. After
  reaching a specific density, cultures can seed a suspension culture
  flask or roller bottle for high volume cell production.


                                                                                           •   With Pourout and Caps
                                                                                           •   Fluted design improves mixing
                                                                                           •   Deep skirted screw caps
                                                                                           •   35 – 1500 mL size
                                                                                           These flasks feature deep-fluted sides to promote mixing. A dam below the
                                                                                           sidearm retains untrypsinized tissue when decanting. A deep skirted screw cap
                                                                                           on the sidearm prevents contamination of flask contents. The sidearm allows
                                                                                           easier decanting of contents while retaining large particles behind the dam.
                                                                                           Sidearm caps are 20-415 size. Caps have white styrene-butadiene rubber
                                                                                           liners. Flasks are made from Wheaton 33 low extractable borosilicate glass that
                                                                                           conforms to USP Type I and ASTM E-438 Type I, Class A requirements.
                                                                                           Cat. No.      Capacity (mL)       Top Screw Cap Size         Flask (mL)          Qty/Case
                                                                                           355752             35                  24-430                    50                    1
                                                                                           355753             75                  33-430                   125                    1
• Fluted design improves mixing                                                            355754            150                  38-430                  250                     1
• 35 – 1500 mL size                                                                        355755            300                  38-430                  500                     1
                                                                                           355757            700                  51-400                  1000                    1
These units have screw caps with white styrene-butadiene rubber liners that                355758            1500                 51-400                  2000                    1
reduce contamination of contents. The fluting is designed to improve mixing.
The flasks are made from Wheaton 33 low extractable borosilicate glass that
conforms to USP Type I and ASTM E-438 Type I, Class A requirements.
Cat. No.     Capacity (mL)              Cap Size               Flask (mL)       Qty/Case
355392            35                    24-430                    50                  1
355393            75                    33-430                    125                 1
355394            150                   38-430                    250                 1
355395            300                   38-430                    500                 1
355397            700                   51-400                   1000                 1
355398           1500                   51-400                   2000                 1




                                                                                           Cat. No. Capacity (mL)   Top Screw Cap Size     Graduations (mL)    Flask (mL)   Qty/Case
                                                                                           355802        35              24-430            Every 5                50              1
                                                                                           355803        75              33-430            10, 25, 50, 75         125             1
                                                                                           355804        150             38-430            50, 75, 100, 150       250             1
                                                                                           355805        300             38-430            80, 100, 150, 300      500             1
                                                                                           355807        700             51-400            Every 100             1000             1
                                                                                           355808       1500             51-400            Every 150             2000             1



Cat. No.   Capacity (mL)     Cap Size       Graduations (mL)       Flask (mL)   Qty/Case
355442         35            24-430         Every 5                    50             1
355443         75            33-430         10, 25, 50, 75            125             1
355444        150            38-430         50, 75, 100, 150          250             1
355445        300            38-430         80, 100, 150, 300         500             1                                     For Hybridization Bottles see page 11.
355447        700            51-400         Every 100                1000             1
355448        1500           51-400         Every 150                2000             1




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51


                                                                                                     speeds, the Celstir flask provides an optimum environment for
                                                                                                     most suspension lines, including insect cell cultures. Hybridomas
         Wheaton Science Products has long been an innovator in cell                                 and adapted cell lines can also be grown in these spinner flasks.
         culture flasks. The Wheaton Magna Flex® impeller produces                                    Sidearms on Magna Flex and Celstir vessels 500 mL and larger
         high-yield cultures by maintaining even circulation while keep-                             were widened to accommodate monitoring and control devices or
         ing cultures in suspension. Slow acceleration and slow stop from                            media inlet/outlet lines.
         the Wheaton Micro-Stir® stirrer prevent cell detachment from
         microcarriers.                                                                              Wheaton has now moved cell culture another step forward with its
                                                                                                     innovative patented MantaRay® single-use culture flask. The
         Wheaton also invented an adjustable impeller flask that does not                             MantaRay comes pre-sterilized to protect cell cultures. The
         have a rod extending through the top of a flask. Celstir® flasks                              MantaRay has a unique impeller that provides efficient, gentle
         feature adjustable blade impeller for increased aeration and                                stirring for both suspension or microcarrier culture. The MantaRay
         dimpled bottom to prevent the accumulation of cells under the                               is disposable; discard it after use and save the time, trouble and
         impeller.*                                                                                  expense of autoclaving and cleaning.
         When used with the Micro-Stir, which can operate at very low                                *Dimpled bottom on 125 mL size and larger.




     MantaRay® pictured with Micro-Stir® which is sold separately.


                                                                                                   • Impeller rod does not protrude up through top cap
                                                                                                   • Sidearms for 500 mL - 8 L flask allow vented caps which enables the flask
     •    Single use spinner flask with integrated magnet                                             to “breathe” while protecting from contamination.
     •    Pre-sterilized making it ready to use out of the box                                     • 1:1 headspace for good gas exchange
     •    Break resistant unit has less potential for culture/sample loss                          • No contamination from stirring rod
     •    One MantaRay generates the same yield as 77 T175 T-flasks conserving                      • Completely autoclavable
          on valuable incubator space and reducing costs associated with laboratory
          consumables                                                                              The Celstir Spinner Flask features an adjustable paddle blade impeller for better
                                                                                                   mixing. The addition of the bottom dimple to flasks 125 mL and larger improves
     The MantaRay’s integrated paddle spinner system is optimized for culturing                    circulation and reduces the accumulation of cells in the center of the flask.
     volumes of 500 mL through 1000 mL. Using a vertically oriented stirrer, the                   Celstirs provide maximum surface interface between culture and flask
     MantaRay ensures that cells are evenly distributed throughout the entire volume               atmosphere. Use the Celstirs for microcarrier and suspension cultures such as
     of culture.                                                                                   insect cells, hybridomas, and adapted cell lines. The entire unit may be
     Unlike conventional spinner flasks, the MantaRay’s paddle system comes                         autoclaved at 121°C for 20 minutes. Celstirs are made from Wheaton 33 low
     ready-to-use, eliminating the need for assembly and disassembly of the device.                extractable borosilicate glass that conforms to ASTM Type I, Class A and USP
     The unique design of the spinner allows for even distribution of nutrients and                Type I requirements. Wheaton also offers a non-breakable stainless steel shaft
     oxygen; thus creating uniform media conditions for cell propagation. The constant             assembly kit as a retrofit to your Celstir (see 356874 series). Units 500 mL and
     agitation caused by the paddle system also prevents cells from settling to the                larger can be used with the Vented Cap (Cat. No. W240751).
     bottom and adhering to the side walls.                                                        Graduations: Sizes 125 to 1000 mL are graduated in 50 mL increments; sizes
     The paddle is integrated with the top plate, which stabilizes the fixed stirrer                3000 to 8000 mL are graduated in 500 mL increments; sizes 25 mL and 50 mL
     height thus eliminating validation concerns due to height adjustments. The paddle             are non-graduated.
     and bag are heat sealed to the top plate reducing potential contamination related              Cat. No.   Volume (mL)            Dia x H (mm)   Cap Size, Top   Sidearm Size   Qty/Case
     to adhesive compounds. The only required pieces of equipment for use of the                    356873          25                 38 x 122         38-430         15-415             1
     MantaRay system are the MantaRay and a magnetic stirrer.                                       356875          50                 38 x 141         38-430         15-415             1
     Use the MantaRay for growing mammalian cell cultures, as well as plant and                     356876         125                 65 x 155         51-400         33-430             1
     insect cells. The MantaRay’s proprietary stirrer technology is designed to minimize            356879         250                 85 x 175         51-400         33-430             1
     damage to fragile cells, such as mammalian. The MantaRay also allows media                     356882         500                110 x 190        100-400         45 mm              1
     and gases to be added or changed during the experiment under sterile conditions.               356884         1000               130 x 250        100-400         45 mm              1
     The MantaRay can culture cells on micro-carrier beads, which the cells use as a                356887         3000               178 x 341        100-400         45 mm              1
     growth surface. The MantaRay comes pre-sterilized. Simply fill the flask with                    356889         6000               258 x 404        100-400         45 mm              1
     media, add cells and let them grow. Use the MantaRay with the Wheaton                          356890         8000               293 x 445        100-400         45 mm              1
     Micro-Stir® slow speed (5-200 RPM) magnetic stirrer. See page 53 for details.
     Cat. No.               Description                       Working Volume (mL)     Qty/Case                              For sidearm replacement caps see page 41.
     W356805-1              MantaRay                                 1000                    1                              For vented caps see page 42.
     W356805-4              MantaRay                                 1000                    4



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                                                                                                                                                                                52




                                                                                         Replacement for Double Sidearm Celstir and Jacketed Double Sidearm
                                                                                         Celstir. This non-breakable stainless steel shaft, for replacing the glass shaft
                                                                                         included with Celstir® flasks, may be retrofitted to the existing Celstir flasks.
                                                                                         Cat. No.                           For Flask Size (mL)                     Qty/Case
                                                                                         356874                                    25                                       1
                                                                                         356877                                    50                                       1
                                                                                         356878                                    125                                      1
                                                                                         356880                                    250                                      1
•   Water jacket allows for precise temperature control                                  356883                                    500                                      1
•   Manufactured from Wheaton 33 low extractable borosilicate glass                      356885                                   1000                                      1
•   Proportioned to provide a head space ratio of 1:1 or greater                         356886                                   3000                                      1
•   The impeller does not protrude through the top cap                                   356888                                   6000                                      1
•   Fully autoclavable                                                                   356891                                   8000                                      1
•   PTFE faced rubber-lined caps on sidearms
The Double-Sidearm Celstir with water jacket allows precise temperature control
of its contents when operated with a recirculating water bath. The hose                  Replacement for Double Sidearm Celstir and Jacketed Double Sidearm Celstir.
connectors accept 1/4 in (6.35 mm) ID tubing. This unit is made from Wheaton             This unit includes a top cap with liners, glass rod, magnet holder, magnet and
33 low extractable borosilicate glass that conforms to USP Type I and ASTM               stirring paddle (for sizes 125 mL or larger).
E-438 Type I, Class A requirements. The 500 and 1000 mL sizes can also be                Cat. No.                           For Flask Size (mL)                     Qty/Case
used with Wheaton Vented Cap Cat. No. W240751.                                           356893                                    25                                       1
Cat. No.   Volume (mL)      Dia x H (mm)       Cap Size, Top Sidearm Size     Qty/Case   356895                                    50                                       1
356943        25            54 x 134             38-430          15-415             1    356896                                    125                                      1
356945        50            54 x 147             38-430          15-415             1    356899                                    250                                      1
356946        125            78 x 162             51-400         33-430             1    356902                                    500                                      1
356949        250           100 x 182             51-400         33-430             1    356904                                   1000                                      1
356952        500           130 x 195            100-400         45 mm              1    356907                                   3000                                      1
356954       1000           150 x 260            100-400         45 mm              1    356909                                   6000                                      1
                                                                                         356910                                   8000                                      1



                                                                                         Replacement for Magna Flex® Spinner Flasks. This unit includes a top cap with
                                                                                         liners, glass stirring ball and connecting silicone tubing. This glass impeller
                                                                                         assembly is designed for replacement on all Magna Flex® style flasks.
                                                                                         Cat. No.                           For Flask Size (mL)                     Qty/Case
                                                                                         356841                                    125                                      1
                                                                                         356842                                    250                                      1
                                                                                         356843                                    500                                      1
                                                                                         356844                                    1000                                     1
                                                                                         356847                                    3000                                     1
                                                                                         356848                                    6000                                     1
                                                                                         356849                                    8000                                     1

• Designed for use with microcarrier cultures
• Side arms for 500 mL - 6 L flasks allow vented caps which enables the
                                                                                         Cat. No.                     For Flask Size (mL)                           Qty/Case
  flask to “breath” while protecting from contamination
• Gentle, even stirring action                                                           Celstir Only
                                                                                         356913                       25 mL Celstir Flask Only                              1
The Wheaton Magna Flex Spinner Flasks feature a flex-type, bulb-shaped glass
                                                                                         356915                       50 mL Celstir Flask Only                              1
impeller that rotates from a fixed position above liquid level around an indentation
                                                                                         356930                       8000 mL Celstir Flask Only                            1
in the base of the flask. Primarily designed for use with microcarrier cultures, this
unique stirring system increases stirring efficiency and provides a gentle, even          Celstir & Magna-Flex
circulation throughout the flask while keeping the beads in suspension.                   356916                       125 mL Celstir/Magna Flex Flask Only                  1
                                                                                         356919                       250 mL Celstir/Magna Flex Flask Only                  1
All flasks have been proportioned to provide a head space ratio of 1:1 or greater.        356922                       500 mL Celstir/Magna Flex Flask Only                  1
A removable stainless steel pin immobilizes the impeller during handling or              356924                       1000 mL Celstir/Magna Flex Flask Only                 1
decanting to prevent damage to cells or microcarriers. Sizes 500 mL and larger           356927                       3000 mL Celstir/Magna Flex Flask Only                 1
can accommodate the Wheaton Vented Cap (Cat. No. W240751).                               356929                       6000 mL Celstir/Magna Flex Flask Only                 1
Cat. No.   Volume (mL)     Dia x H (mm)        Cap Size, Top   Sidearm Size   Qty/Case   Jacketed Celstir Flask
356830        125            65 x 155             51-400         33-430             1    356963                       25 mL Jacketed Celstir Flask Only                     1
356831        250            85 x 175             51-400         33-430             1    356965                       50 mL Jacketed Celstir Flask Only                     1
356832        500           110 x 190            100-400         45 mm              1    356966                       125 mL Jacketed Celstir Flask Only                    1
356834       1000           130 x 250            100-400         45 mm              1    356969                       250 mL Jacketed Celstir Flask Only                    1
356837       3000           178 x 341            100-400         45 mm              1    356972                       500 mL Jacketed Celstir Flask Only                    1
356839       6000           258 x 404            100-400         45 mm              1    356974                       1000 mL Jacketed Celstir Flask Only                   1


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53



                                                                                                                 • 5-200 RPM
                                                                                                                 Designed specially for cell culture applications requiring exceptionally slow
                                                                                                                 speeds, gentle mixing preventing shear and low heat transfer. May be used in
                                                                                                                 an environment with CO2 and non-condensing humidity, and with a temperature
                                                                                                                 range to 50°C. Packaged 1 unit per case.
                                                                                                                 Cat. No.                   Plug Style
                                                                                                                 Single Place Micro-Stir®
                                                                                                                 W900700-A                  120 VAC, North America
                                                                                                                 W900700-B                  100 VAC, Japan
                                                                                                                 W900700-C                  230 VAC, Continental Europe
     Single Place BioStir                                                                                        W900700-D                  230 VAC, United Kingdom
                                                                                                                 W900700-F                  240 VAC, Australia/China
                                                                                                                 W900700-G                  230 VAC, Italy/Chile
                                                                                                                 W900700-J                  230 VAC, India
                                                                                                                 Four Place Micro-Stir®
                                                                                                                 W900701-A                  120 VAC, North America
                                                                                                                 W900701-B                  100 VAC, Japan
                                                                                                                 W900701-C                  230 VAC, Continental Europe
                                                                                                                 W900701-D                  230 VAC, United Kingdom
                                                                                                                 W900701-F                  240 VAC, Australia/China
                                                                                                                 W900701-G                  230 VAC, Italy/Chile
                                                                                                                 W900701-J                  230 VAC, India
     Four Place Micro-Stir


                                                                                                                 • 150-1200 RPM
                                                                                                                 Designed specifically for use with suspension culture flasks. The effortless
     • Computerized stirrer control                                                                              motor provides uniform speeds for great results with cell culture or small
     • Rotation speed clearly visible for consistent results                                                     fermentations. An easy-to-read display allows exact RPM to be set. The smooth-
     • Variety of operating modes for suspension or microcarrier cultures                                        running motor reduces heat transfer to medium being stirred. Takes flask sizes
            Constant Speed Mode - Soft start to a constant stirring speed,                                       to 3 Liters. May be used in an environment with CO2 and non-condensing
            this mode is used for suspension cultures.                                                           humidity, and with a temperature range to 50°C. Packaged 1 unit per case.
                                                                                                                 Cat. No.                   Plug Style
            Interval Start Mode - This mode turns the stirrer on and off using the
            soft start and stop for a set number of cycles, after which the stirrer                              Single Place BioStir®
            runs at a constant speed that may differ from the speed used to attach                               W900702-A                  120 VAC, North America
            the cells. This mode is useful for attaching cells to micro-carrier beads.                           W900702-B                  100 VAC, Japan
            There is no need to watch the culture and turn on the stirrer for a                                  W900702-C                  230 VAC, Continental Europe
            constant stir mode after the cells have attached.                                                    W900702-D                  230 VAC, United Kingdom
                                                                                                                 W900702-F                  240 VAC, Australia/China
          2-Speed Stirring Mode - The stirrer slowly starts to a set speed, which is
                                                                                                                 W900702-G                  230 VAC, Italy/Chile
          held for a user-defined period. The stirrer then softly changes to a final
                                                                                                                 W900702-J                  230 VAC, India
          constant speed. This mode is also useful for microcarrier applications.
                                                                                                                 Four Place BioStir®
     • 2 year warranty
                                                                                                                 W900703-A                  120 VAC, North America
     • Conforms to UL, CSA, and CE Standards
                                                                                                                 W900703-B                  100 VAC, Japan
     • Optional remote control
                                                                                                                 W900703-C                  230 VAC, Continental Europe
     Specifications:                                                                                              W900703-D                  230 VAC, United Kingdom
     Electrical:                                                                                                 W900703-F                  240 VAC, Australia/China
     Operating voltage: .....................................................100-240 VAC, 50/60 Hz               W900703-G                  230 VAC, Italy/Chile
     Power consumption: ........................................................................ 15 Watts        W900703-J                  230 VAC, India
     Pollution Degree: ............................................................................... Class 2
     Environmental:
     Operating temperature: ............................................................. 15°C to 40°C
     Humidity: .........................................................80% up to 31°C, 50% at 40°C                                                        • For BioStir® and Micro-Stir®
     Altitude limit: ............................................................................. 2000 meters                                               Magnetic Stirrers
     Operational:
     Operating speed:
            Micro-Stir: ........................................................................ 5 - 200 RPM
            BioStir: ....................................................................... 150 - 1200 RPM
     Maximum flask size: .........................................................................3 L flask
     Dimensions: H x W x D                                                                                       The Wheaton Remote Control allows all stirrer functions to be controlled and
     Single Place: .................................... 3.6 x 8.0 x 9.8 in (9.2 x 20.3 x 24.9 cm)                monitored without opening an incubator and disturbing the incubator contents.
     Four Place: ..................................4.0 x 15.5 x 17.5 in (10.2 x 39.4 x 44.5 cm)                  The remote control comes with a 12 ft. (3.66 m) cord which allows the stirrer to
     Weight:                                                                                                     be placed in an incubator (such as the Wheaton roll-in or CO2 Incubators) while
     Single Place: ........................................................................... 4.5 lb (1.9 kg)   allowing freedom of placement for the remote controller.
     Four Place: .............................................................................. 10 lb (4.5 kg)
                                                                                                                 Cat. No.                 Description                                       Qty/Case
                                                                                                                 W900704                  Remote Control                                          1


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                                                                                                                                                                                                     54

                                                                                                         Cat. No.                   Plug Style                               Voltage      Qty/Case
                                                                                                                                                  ®
                                                                                                         250 mL Cell Optimizer System
                                                                                                         W350000-A                  North America                            120 VAC            1
                                                                                                         W350000-B                  Japan                                    100 VAC            1
                                                                                                         W350000-C                  Continental Europe                       230 VAC            1
                                                                                                         W350000-D                  United Kingdom                           230 VAC            1
                                                                                                         W350000-F                  Australia/China                          240 VAC            1
                                                                                                         W350000-G                  Italy/Chile                              230 VAC            1
                                                                                                         W350000-J                  India                                    230 VAC            1
                                                                                                         500 mL Cell Optimizer System®
                                                                                                         W350010-A                  North America                            120 VAC            1
                                                                                                         W350010-B                  Japan                                    100 VAC            1
                                                                                                         W350010-C                  Continental Europe                       230 VAC            1
                                                                                                         W350010-D                  United Kingdom                           230 VAC            1
                                                                                                         W350010-F                  Australia/China                          240 VAC            1
                                                                                                         W350010-G                  Italy/Chile                              230 VAC            1
                                                                                                         W350010-J                  India                                    230 VAC            1
                                                                                                         1 Liter Cell Optimizer System®
                                                                                                         W350020-A                  North America                            120 VAC            1
                                                                                                         W350020-B                  Japan                                    100 VAC            1
                                                                                                         W350020-C                  Continental Europe                       230 VAC            1
                                                                                                         W350020-D                  United Kingdom                           230 VAC            1
                                                                                                         W350020-F                  Australia/China                          240 VAC            1
                                                                                                         W350020-G                  Italy/Chile                              230 VAC            1
                                                                                                         W350020-J                  India                                    230 VAC            1
Key areas of use are:
                                                                                                         3 Liter Cell Optimizer System®
          •   Small culture bioreactor                                                                   W350030-A                  North America                            120 VAC            1
          •   Cell culture process optimization                                                          W350030-B                  Japan                                    100 VAC            1
          •   Insect cell culture techniques                                                             W350030-C                  Continental Europe                       230 VAC            1
          •   Hybridoma cell culture methods                                                             W350030-D                  United Kingdom                           230 VAC            1
          •   Viral infection studies                                                                    W350030-F                  Australia/China                          240 VAC            1
The Cell Optimizer System® is a complete, small-volume cell culture bioreactor.                          W350030-G                  Italy/Chile                              230 VAC            1
This system provides a quick and accurate determination of optimum                                       W350030-J                  India                                    230 VAC            1
environmental cell growth parameters. Cell growth in the Cell Optimizer System                           For more information on plug styles see page 188.
takes place in a 250 mL, 500 mL, 1 liter or 3 liter working volume spinner flask
with four (4) sidearms.
The system includes a Control Tower™, Control Tower Support Unit and
Micro-Stir® magnetic stirrer. The Control Tower and support unit automatically
controls pH via base addition and CO2 gas, and dissolved oxygen utilizing air, O2                        Cat. No.                          Description             Voltage                Qty/Case
and N2 gases.                                                                                            WI055902                          250 mL Spinner Flask with 2 Sidearms                 1
Temperature is maintained by the use of a heating pad that eliminates the need                           356922                            500 mL Spinner Flask with 2 Sidearms                 1
for an incubator. Stirring speed (5-200 RPM) is achieved by manually setting                             WL054377                          1 L Spinner Flask with 4 Sidearms                    1
the Micro-Stir® magnetic stirrer. Foam and nutrient feed control are available                           WL054376                          3 L Spinner Flask with 4 Sidearms                    1
as options. The support unit includes a combination heater/pump module for
temperature control and liquid base addition. A four-gas mixing module provides
controlled additions of necessary gases to the vessel head space (and/or optional                        This 125 watt heating pad plugs into the Support Unit and is controlled by the
sparger). One year warranty.                                                                             Control Tower™. Dimensions: 12.7 x 12.7 cm.
                                                                                                         Cat. No.              Description             Voltage                            Qty/Case
For more specifics on the Control Tower see page 56.
                                                                                                         I057638               Heating pad             100-120 VAC, 50/60 Hz, 125 watts         1
Probe Specifications:                                                                                     I057599               Heating pad             220-240 VAC, 50/60 Hz, 125 watts         1
Temperature: ............................................................................ 100 Ohm RTD
Dissolved Oxygen: ........................................................ Polarographic, 12 mm
pH: ................................................................... Pressurized, Gel Filled, 12 mm   The 316 L stainless steel sampler is designed to work with either the 1 or 3 liter
Units come configured for temperature, pH and dissolved oxygen. Other                                     flask. The sampler is supplied with a plug valve to help maintain sterility of the
configurations are available upon request. For ease of record keeping and                                 vessel during sampling.
supervisory control, up to 32 Cell Optimizer System setups can be networked to                           Cat. No.                     Description                                         Qty/Case
the optional BIOPRO® PC supervisory software. See page 57 for details.                                   I057501                      Sampler with plug valve, 30.5 cm                          1



                                                                                                                                                 The 316 L stainless steel tubular cold finger may
                                                                                                                                                 be used with either the 1 or 3 liter flask.




                                                                                                         Cat. No.                     Description                                         Qty/Case
                                                                                                         I057459                      Cold Finger                                               1
                                             Due to specific country requirements, the Cell Optimizer is not available for sale in Canada and Europe.

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55


                                                                                                              19 Liter Mini-Pilot Plant™ System with Control Tower™ configured for
                                                                                                              temperature, pH and dissolved oxygen; Support Unit with a heater/pump module
                                                                                                              and a four-gas mixing module; Overdrive™ stirrer speed control (20-150 RPM);
                                                                                                              19 liter vessel with six sidearms; temperature, pH and dissolved oxygen probes
                                                                                                              and cables; sparger; heating blanket; filters; and a 1 liter storage bottle (for base
                                                                                                              addition). This unit comes with one of seven standard electrical plugs. Additional
                                                                                                              configurations are available through special order.
                                                                                                              Cat. No.                      Plug Style                        Voltage               Qty
                                                                                                              W350050-A                     North America                     120 VAC                 1
                                                                                                              W350050-B                     Japan                             100 VAC                 1
                                                                                                              W350050-C                     Continental Europe                230 VAC                 1
                                                                                                              W350050-D                     United Kingdom                    230 VAC                 1
                                                                                                              W350050-F                     Australia/China                   240 VAC                 1
                                                                                                              W350050-G                     Italy/Chile                       230 VAC                 1
                                                                                                              W350050-J                     India                             230 VAC                 1

     Key areas of use are:
             • Large volume cell culture bioreactor
             • Scale cultures up to production volume
             • Antibody or virus production                                                                   The 45 Liter Mini-Pilot Plant™ System with Control Tower™ is configured for
             • Pilot plant studies                                                                            temperature, pH and dissolved oxygen; a Support Unit with a heater/pump
             • Economical mass cell production                                                                module and a four-gas mixing module; Overdrive™ stirrer speed control (20-150
             • Production of proteins or other cell products                                                  RPM); 45 liter vessel with seven sidearms; temperature, pH and dissolved oxygen
                                                                                                              probes and cables; sparger; heating blanket; filters; and a 1 liter storage bottle
     The Mini-Pilot Plant™ spinner flask system is a large-volume cell culture                                 (for base addition). This unit comes with one of seven standard electrical plugs.
     bioreactor designed to scale cultures up to production volumes of cells or                               Additional configurations are available through special order.
     products. The system includes a Control Tower™, Support Unit, Overdrive™,
                                                                                                              Cat. No.                      Plug Style                        Voltage               Qty
     heating blanket, sparger, and a choice of a 10 liter (six sidearms), 19 liter (six
     sidearms) or 45 liter (seven sidearms) vessel. The Control Tower™ and                                    W350060-A                     North America                     120 VAC                 1
     Support Unit automatically control pH via base addition and CO2 gas, and                                 W350060-B                     Japan                             100 VAC                 1
     dissolved oxygen utilizing air, O2 and N2 gases. For more specifics on the Control                        W350060-C                     Continental Europe                230 VAC                 1
     Tower see page 56.                                                                                       W350060-D                     United Kingdom                    230 VAC                 1
                                                                                                              W350060-F                     Australia/China                   240 VAC                 1
     Temperature is maintained by the use of a heating blanket that eliminates                                W350060-G                     Italy/Chile                       230 VAC                 1
     the need for an incubator. Stirring speed is achieved by manual setting of the                           W350060-J                     India                             230 VAC                 1
     Overdrive system. Foam and nutrient feed control are available as options. The                           For more information on plug styles see page 188.
     Support Unit includes a combination heater/pump module for temperature control
     and liquid base addition. A four-gas mixing module provides controlled additions
     of necessary gases to the vessel headspace and sparger. One year warranty.
     Probe Specifications:
                                                                                                              The heating wrap plugs into the support unit and is controlled by the Control
     Temperature: ........................................................................... 100 Ohm RTD     Tower™.
     Dissolved Oxygen: ........................................................ Polarographic, 12 mm          Cat. No.              Description                          Voltage               Qty/Case
     pH: ................................................................... Pressurized, Gel Filled, 12 mm
                                                                                                              WI055974              Heating Wrap, 10 L            100-120 VAC, 700 watts              1
     For ease of record keeping and supervisory control, up to 32 Mini Pilot System                           WI055975              Heating Wrap, 10 L            220-240 VAC, 700 watts              1
     setups can be networked to the optional BIOPRO® PC supervisory software. See                             I057152               Heating Wrap, 19 L            100-120 VAC, 700 watts              1
     page 57 for details.                                                                                     I057621               Heating Wrap, 19 L            220-240 VAC, 700 watts              1
     For more information on plug styles see page 188.
                                                                                                              I057153               Heating Wrap, 45 L            100-120 VAC, 960 watts              1
                                                                                                              I057622               Heating Wrap, 45 L            220-240 VAC, 960 watts              1



     The 10 liter Mini-Pilot Plant™ System comes with a Control Tower™ configured for                                                              The 316 L stainless steel sampler is designed to
     temperature, pH and dissolved oxygen; Support Unit with a heater/pump module                                                                 work with the 10, 19 or 45 liter Mini-Pilot Plant™. The
     and a four-gas mixing module; Overdrive™ stirrer speed control (20-150 RPM);                                                                 sampler is supplied with a plug valve to help maintain
     10 liter vessel with six sidearms; temperature, pH and dissolved oxygen probes                                                               sterility of the vessel during sampling.
     and cables; sparger; heating blanket; filters; and a 1 liter storage bottle (for base
     addition). This unit comes with one of seven standard electrical plugs. Additional                       Cat. No.                              Description                                Qty/Case
     configurations are available through special order.                                                       I057502                              Sampler with plug valve, 48.3 cm                   1
     Cat. No.                      Plug Style                             Voltage                      Qty
     W350040-A                     North America                          120 VAC                        1
     W350040-B                     Japan                                  100 VAC                        1
     W350040-C                     Continental Europe                     230 VAC                        1                                  The 316 L stainless steel tubular cold finger may be used
     W350040-D                     United Kingdom                         230 VAC                        1                                  with the 10, 19 or 45 liter Mini-Pilot Plant™.
     W350040-F                     Australia/China                        240 VAC                        1
     W350040-G                     Italy/Chile                            230 VAC                        1
     W350040-J                     India                                  230 VAC                        1
                                                                                                              Cat. No.                              Description                                Qty/Case
                                                                                                              I057617                              Cold Finger                                        1
                                                  Due to specific country requirements, the Mini-Pilot Plant is not available for sale in Canada and Europe.

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                                                                                                                                                                                                                  56




                                                                                                             • 1 year warranty
                                                                                                             This complete system comes with the vessel, impeller
                                                                                                             assembly and top drive motor. (When ordering, please
                                                                                                             specify 10, 19 or 45 liter vessels to provide working
                                                                                                             volumes of 8.5, 16 or 36 liters respectively). The large,
                                                                                                             adjustable height PTFE paddle (part of the impeller
                                                                                                             assembly) works together with the dimple in the
                                                                                                             bottom of the flask to provide gentle, even distribution
                                                                                                             and reduce dead areas. The impeller’s wetted compo-
                                                                                                             nents include 316 L stainless steel, virgin PTFE and Rulon® 641. Rulon 641 is a
                                                                                                             specially formulated compound of virgin PTFE and fillers approved by the FDA for
                                                                                                             use as a food component and thus for direct contact with food and drugs intended
• Monitor and control up to eight operation parameters                                                       for oral consumption. The vessel is equipped with six or seven 45 mm sidearms to
• Monitor and control parameters in multiple vessels                                                         accommodate probes for monitoring and control, and tubing for sparging or media
• Network up to 32 Control Tower™ units                                                                      addition. The vessel and impeller sections are autoclavable; the lift-off top drive
                                                                                                             section is not autoclavable. This unit comes with one of seven standard electrical
The Control Tower Digital Control System is a versatile and compact system for                               plugs. Additional configurations are available through special order.
mammalian cell culture and insect cell culture. The system provides
bench-scale monitoring and PID control of operation parameters. The Control                                  Electrical Requirements: ...................................100-120 VAC, 60Hz, 30 watts
Tower and Support Unit can be factory configured for any combination of the                                                                                            220-240 VAC, 50/60 Hz, 30 watts
following parameters:                                                                                        Dimensions D x H (Vessel and Overdrive™ together):
         • pH                                                                                                    10 Liter System: ............................................................... 23 cm x 64 cm
         • Dissolved oxygen                                                                                      19 Liter System: ............................................................... 29 cm x 67 cm
         • Temperature                                                                                           45 Liter System: ............................................................... 41 cm x 78 cm
         • Anti-foam                                                                                         Cat. No.                       Plug Style                          Voltage              Qty/Case
         • Nutrient feed
                                                                                                             10 Liter System
To further enhance the Control Tower, the BIOPRO® PC software (see page 57)                                  W356700-A                      North America                      120 VAC                       1
provides sophisticated data acquisition, expanded process control capability and                             W356700-B                      Japan                              100 VAC                       1
the ability to network together up to 32 systems. The Control Tower allows you to                            W356700-C                      Continental Europe                 230 VAC                       1
set and confirm setpoints and PID values, calibrate temperature, pH and DO                                    W356700-D                      United Kingdom                     230 VAC                       1
sensors, and set times for feed pump operations. Monitor parameters and data                                 W356700-F                      Australia/China                    240 VAC                       1
entry by scrolling through the menus using the front panel keypad. All data is                               W356700-G                      Italy/Chile                        230 VAC                       1
displayed on a two-line LCD. All cable connections for sensors and external                                  W356700-J                      India                              230 VAC                       1
devices (e.g., Support Unit) are on the rear of the unit.                                                    19 Liter System
Specifications:                                                                                               356420-A                       North America                      120 VAC                       1
                                                                                                             356420-B                       Japan                              100 VAC                       1
Operating Voltage: ..................................... 100-120 VAC, 50/60 Hz, 10 watts                     356426-C                       Continental Europe                 230 VAC                       1
                                                              220-240 VAC, 50/60 Hz, 10 watts                356426-D                       United Kingdom                     230 VAC                       1
Dimensions: ............................................................... 16.5 x 22.8 x 38.7 cm            356426-F                       Australia/China                    240 VAC                       1
Weight: ................................................................................. 4.5 kg (9.9 lbs)   356426-G                       Italy/Chile                        230 VAC                       1
                                                                                                             W356426-J                      India                              230 VAC                       1
                                                                                                             45 Liter System
                                                                                                             356422-A                       North America                      120 VAC                       1
                                        Modular design allows for any combination of                         356422-B                       Japan                              100 VAC                       1
                                        pumps, gas mixing and heating control The modu-                      356428-C                       Continental Europe                 230 VAC                       1
                                        lar support unit adds automated reagent pumping,                     356428-D                       United Kingdom                     230 VAC                       1
                                        gas mixing and heating capabilities to support the                   356428-F                       Australia/China                    240 VAC                       1
                                        Control Tower™ in maintaining bioreactors. A single                  356428-G                       Italy/Chile                        230 VAC                       1
                                        support unit can have up to four modules to con-                     W356428-J                      India                              230 VAC                       1
                                        serve bench space. The following modules can be                      Top Drive Motor Only
                                        combined in the factory to create the support unit:                  356424-A                       North America                      120 VAC                       1
                                              • Peristaltic pump module for automatic                        356424-B                       Japan                              100 VAC                       1
                                                addition of liquid acid, base, antifoam or                   356430-C                       Continental Europe                 230 VAC                       1
                                                nutrients, with or without heater option                     356430-D                       United Kingdom                     230 VAC                       1
                                              • Four-gas mixing module for cell culture                      356430-F                       Australia/China                    240 VAC                       1
                                                (air, O2, CO2, N2 gas); flow rate 0-500 cc/min                356430-G                       Italy/Chile                        230 VAC                       1
                                                                                                             W356430-J                      India                              230 VAC                       1
                                              Specifications:
                                              Operating Voltage:
                                              100-120 VAC, 50/60 Hz, 1000 watts maximum                      Cat. No.                Description                                                     Qty/Case
                                              220-240 VAC, 50/60 Hz, 1000 watts maximum                      356433                  Impeller Assembly, 45 L                                                 1
                                              Dimensions: 16.5 x 16.5 x 19.7 cm*                             WL052594                19 L Vessel with 6 Sidearms without Caps                                1
                                              *Base & 1 module; each additional module add 9.5 cm            356471                  19 L Vessel with 6 Sidearms with Caps                                   1
                                                                                                             I050567                 45 L Vessel with 7 Sidearms without Caps                                1
The Control Tower Control System and Support Unit are integrated with the
Cell Optimizer and Mini-Pilot Plant System; however, they are available                                      W356473                 45 L Vessel with 7 Sidearms with Caps                                   1
separately upon request. Contact Wheaton for more details.                                                   WL055973                10 L Vessel with 6 Sidearms                                             1

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57




      The BIOPRO®PC software is designed to extend the operational capabilities of the          variable channels and multi-tasking capability. The computer can be used for other
      Control Tower™ System and Support Unit. This Microsoft® Windows® based software           operations while monitoring and recording data. All data stored in the computer is
      provides both supervisory control and monitoring of key process parameters, along         compatible with Microsoft Excel® for further analysis.
      with data archiving functions.
                                                                                                Consistent batch-to-batch results can be achieved by directly downloading batch
      BIOPRO® PC Software features:                                                             recipes from the software to the Control Tower.
               • Enhanced programmable control and data acquisition capability                  The “timed events” function allows for automatic extension of up to 200 steps. For
                 for the Control Tower local controller                                         added security, two levels of password protection are available.
               • Networking of up to 32 Control Tower units for central supervisory
                 control and data acquisition                                                   BIOPRO® PC Monitor and Control Software
               • Permanent documentation for batch records                                      Includes: RS-232 to RS-485 adapter box with power module and appropriate line cord.
               • Works with Microsoft Windows NT, 2000 and XP                                   100-120 VAC, 220-240 VAC 50/60 Hz
               • Continuous graphic display
                                                                                                This unit comes with one of seven standard electrical plugs. Additional configurations
               • Trend graphing
                                                                                                are available through special order.
               • Process sequencing
               • Password protection
               • Minimal operator training requirement                                          Cat. No.                         Plug Style                Voltage                   Qty
                                                                                                W350070-A                        North America            120 VAC                       1
      The software stores all measured parameters and records all changes made to
                                                                                                W350070-B                        Japan                    100 VAC                       1
      the system. Alarm conditions that occur during a run are highlighted and flagged.
                                                                                                W350070-C                        Continental Europe       230 VAC                       1
      Externally measured variables such as total cell counts, nutrient and metabolic levels,
      and off-gas analysis data can be manually entered into the log for documentation and      W350070-D                        United Kingdom           230 VAC                       1
      data analysis.                                                                            W350070-F                        Australia/China          240 VAC                       1
                                                                                                W350070-G                        Italy/Chile              230 VAC                       1
      A continuous graphic display of system data allows for ease of monitoring while           W350070-J                        India                    230 VAC                       1
      a permanent record is created for use in process validation or batch records. The         For more information on plug styles see page 188.
      BIOPRO®PC software offers trend graphing with user-adjustable data acquisition rates,




     For              see Wheaton Cryule® Cryogenic                                             For                               see the Wheaton Glass
     Vials on pages 140-142 and Vial Racks on page 149.                                         Media Bottles on page 12 and page 30 for Plastic Media Bottles.
                                WHEATON         1.800.225.1437 (U.S. & Canada only) | +1.856.825.1100 | +1.856.825.1368 (F) | www.wheatonsci.com
                                                                                                                                                                                                                                58




Pictured with Two Additional Deck Kits                                                                          Pictured with Two Additional Deck Kits




•   Allows precise rotation speed                                                                               •   Use with glass roller bottles
•   Small scale mixing and agitation                                                                            •   Accomodates larger roller bottles
•   Small footprint                                                                                             •   Small footprint
•   Conforms to UL, CSA, and CE Standards                                                                       •   Conforms to UL, CSA, and CE Standards
                                                                                                                •   1 year warranty
•   1 year warranty
Perfect for Wheaton 30 mL or larger serum bottles, 100-125 mL media bottles                                     This conventional-type roller culture rack is designed to roll vessels 108 to 121
or 38 x 200 mm culture tubes for the growth and observation of various cell                                     mm in diameter and up to 290 mm long. The two outside rollers can be moved
cultures such as, single chicken or rat embryos. The Wheaton Mini Bottle Roller                                 inward to accommodate bottles as small as 75 mm in diameter. Each deck can
Culture Apparatus accommodates bottles 38 to 60 mm in diameter, up to 240                                       accommodate two vessels. Additional decks available separately. See information
mm long, with bottle speeds of 3 to 45 RPM (38 mm bottle) and 2 to 30 RPM                                       on Deck Kit below.
(60 mm bottle). Additional decks available separately. See information on Deck
Kit below.
                                                                                                                Electrical Requirements: .............................. 100-240 VAC, 50/60 Hz, 14 watts
                                                                                                                Bottle Size Accepted: ........................................... 108 to 121 mm (maximum)
                                                                                                                Bottle Speed:
Electrical Requirements: ............................ 100-240 VAC, 50/60 Hz, 14 watts                                    110 mm Diameter Bottle ............................................. 0.1 to 3.8 RPM
Bottle Size Accepted: ..................................38-60 mm x 240 mm (maximum)                             Dimensions: W x D x H ............ 12 1/4” x 12 3/4” x 7 1/8” (32 x 32.5 x 18 cm)
Bottle Speed:                                                                                                   Weight: ........................................................................................ 18 lb (8 kg)
        38 mm Diameter Bottle ................................................... 3 to 45 RPM                   Maximum temperature for use: ............................................................. 40°C
        60 mm Diameter Bottle ................................................... 2 to 30 RPM
Dimensions: W x D x H .............12 1/4” x 12 3/4” x 7 1/8” (32 x 32.5 x 18 cm)                               Warning: Use in a CO2 environment may reduce motor brush life.
Weight: ........................................................................................ 18 lb (8 kg)
Maximum temperature for use: ........................................................... 40°C
Warning: Use in a CO2 environment may reduce motor brush life.                                                  Cat. No.                       Plug Style                                Voltage                  Qty/Case
                                                                                                                W348924-A                      North America                            120VAC                             1
                                                                                                                W348924-B                      Japan                                    100 VAC                            1
Cat. No.                     Plug Style                             Voltage                       Qty/Case      W348924-C                      Continental Europe                       230 VAC                            1
                                                                                                                W348924-D                      United Kingdom                           230 VAC                            1
W348923-A                    North America                          120 VAC                                 1
                                                                                                                W348924-F                      Australia/China                          240 VAC                            1
W348923-B                    Japan                                  100 VAC                                 1
                                                                                                                W348924-G                      Italy/Chile                              230 VAC                            1
W348923-C                    Continental Europe                     230 VAC                                 1
                                                                                                                W348924-J                      India                                    230 VAC                            1
W348923-D                    United Kingdom                         230 VAC                                 1
W348923-F                    Australia/China                        240 VAC                                 1   For more information on plug styles see page 188.
W348923-G                    Italy/Chile                            230 VAC                                 1
W348923-J                    India                                  230 VAC                                 1
For more information on plug styles see page 188.                                                               Small Bottle Deck Kit can be added to Small Bottle Roller Culture Apparatus to
                                                                                                                accommodate an additional two bottles per deck. Can add up to a maximum of
                                                                                                                2 kits per unit. Weight: 9 lb (4.1 kg)
Mini Bottle Deck Kit can be added to Mini Bottle Roller Culture Apparatus to                                    Cat. No.                       Description                                                       Qty /Case
accommodate an additional four bottles per deck. Can add up to a maximum of 2                                   W348930-CH                     Deck Kit for Small Bottle Bench-Top Roller Rack                             1
kits per unit. Weight: 9 lb (4.1 kg)
Cat. No.                      Description                                                         Qty/Case
W348920-CH                    Deck Kit for Mini Bottle Bench-Top Roller Rack                                1




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59




     The Original High Volume                                      Superior Product Designed to                                                    The State of the Art High
     Production Apparatus                                          Maximize Production Spacing                                                     Volume Production Roller
                                                                                                                                                   Apparatus


                                    Cost Buster                            Modular Line                                         R2P Roller System
         Number of Bottle              5 or 8                                      5                                                     5 or 8
         Positions
         Distance           Production Spacing                  Modular Spacing                          6 in (15.24 cm) Production spaced (available in 5 or 8 positions per
         Between            6” (15.2 cm)                        7 1/8” (18.1 cm)                         deck)
         Decks                                                                                           7 1/8 in (18.1 cm) Modular spaced (available in 5 positions per deck)

         Number of          11                                  1 to 9 (easily modified)                  1 - 9 (7 1/8 in. (18.1 cm) Modular spaced-5 positions per deck)
         Decks                                                                                           1 - 11 (6 in (15.24 cm) Production spaced-5 positions per deck)
                                                                                                         11 (6 in (15.24 cm) Production spaced-8 positions per deck)

         Unit Height        Fixed                               Adjustable based on number of            5 position models are adjustable based on number of decks
                                                                decks                                    8 position model is fixed


                            52 (5 bottles/deck) with     01 (min) with                                   1 - 55 (for 5 positions/deck-production spaced) with
         Number of
         Bottles                                 }
                            86 (8 bottles/deck 1700 cm 45 (max) 1700 cm
                                                bottle
                                                       2

                                                                  bottle
                                                                           }
                                                                           2
                                                                                                         1 - 45 (for 5 positions/deck-modular spaced)     }
                                                                                                                                                         1700 cm2
                                                                                                                                                         bottle
                                                                                                                   with
                                 }
                            104 with
                            172 850 cm2 bottle
                                                          1 with
                                                                    }
                                                         90 850 cm2 bottle
                                                                                                         1 - 110
                                                                                                         1 - 90  } 850 cm2 bottle

         Rotation Alarm     Optional                            Optional                                 Standard



         Battery Backup     Optional                            Optional                                 Optional



         Control/Operating Not Available                        Not Available                            CART2 software
         System




                                                     Please note that Roller Culture Apparatus is made to order and not returnable.


                            WHEATON        1.800.225.1437 (U.S. & Canada only) | +1.856.825.1100 | +1.856.825.1368 (F) | www.wheatonsci.com
                                                                                                                                                                                                                                60


                                                     •   Maximum production capacity in a minimum amount of space
                                                     •   Distance between decks is 6” (15.2 cm)
                                                     •   Available in 52 and 86 position models
                                                     •   Drive units mounted on top to provide easy access for maintenance
                                                         and better air flow
                                                                                                                                                           Specifications:
                                                     •   Optional rotation alarm and battery backup systems available
                                                                                                                                                           Electrical: .......100 - 120VAC, 50/60Hz, 35 watts
                                                     •   Greaseless bearings prevent leaching and eliminate bearing seizures
                                                                                                                                                           ........................220 - 240VAC, 50/60Hz, 35 watts
                                                     •   Soft start feature minimizes cell disruption
                                                     •   Conforms to UL, CSA, and CE Standards                                                             Bottle Speed: ....0.25 – 5.3 RPM (110 mm bottle)
                                                     •   1 year warranty
                                                                                                                                                           Operating Temp.: ..............................15 to 40°C
                                                     The Wheaton Cost Buster High Volume Production Roller Apparatus
                                                                                                                                                           Humidity: .................................. 80% up to 31°C
                                                     provides the maximum amount of potential cell culture growth area avail-
                                                     able in the smallest amount of space. This is accomplished by reducing the                            Altitude Limit: ................................ 2000 meters
                                                     spacing between decks. Controls and drive units are located on top of the
                                                     unit, providing easy access for maintenance. The open frame design allows                             Dimensions W x D X H:
                                                                                                                                                              52-Position: ...............30.9 x 24.3 x 74.5 (in)
                                                     better air flow, which minimizes top-to-bottom temperature gradients.
                                                                                                                                                              52-Position: ...........78.4 x 61.6 x 189.4 (cm)
                                                     Wheaton Cost Busters can accommodate roller bottles from 110 to 121                                        86-Position: ...............46.7 x 24.3 x 74.6 (in)
                                                     mm in diameter and up to 550 mm in length. Each roller is driven by                                        86-Position: .........118.7 x 61.6 x 189.4 (cm)
                                                     nonslip belts and pulleys for positive traction (especially important for use
                                                     with lightweight plastic bottles).                                                                    Weight:
The Cost Buster High Volume Production Roller Apparatus is supplied without roller bottles.                                                                   52-Position: ...................... 250 lb (113.4 kg)
                                                                                                                                                              86-Position: ...................... 350 lb (158.8 kg)

Cat. No.                      Plug Style                            Voltage                       Qty/Case       Cat. No.                     Plug Style                           Voltage                       Qty/Case
52-Position Cost Buster                                                                                          86-Position Cost Buster
349000-A                      North America                        120 VAC                                 1     348990-A                     North America                        120 VAC                                1
349000-B                      Japan                                100 VAC                                 1     348990-B                     Japan                                100 VAC                                1
349005-C                      Continental Europe                   230 VAC                                 1     348995-C                     Continental Europe                   230 VAC                                1
349005-D                      United Kingdom                       230 VAC                                 1     348995-D                     United Kingdom                       230 VAC                                1
349005-F                      Australia/China                      240 VAC                                 1     348995-F                     Australia/China                      240 VAC                                1
349005-G                      Italy/Chile                          230 VAC                                 1     348995-G                     Italy/Chile                          230 VAC                                1
W349005-J                     India                                230 VAC                                 1     W348995-J                    India                                230 VAC                                1
Offered with 7 standard electrical plug choices. Additional configurations are available through special order.   For more information on plug styles see page 188.




• Protects cells during power outages                                                                            • Monitors drive system
• Provides a minimum of 24 hours of auxiliary power                                                              • Alarm sound if any failure is detected
• Can be used to rotate vessels during transport to harvest area                                                 • Alarm is visual & audible
Protects your cells and process! The Wheaton Battery Backup System is designed                                   The Wheaton Rotation Alarm System is designed to monitor the drive system on
to be installed in either the Modular or Cost Buster Roller Culture Apparatus. The                               the Modular and Cost Buster Production Roller Culture Apparatus. When the sys-
system monitors the AC power and automatically switches between battery power                                    tem detects a failure it alarms. The alarm is visual (flashing LEDs), audible (loud
and AC power as needed. The system will provide 24 hours of auxiliary power to                                   buzzer), and can be tied into an existing equipment monitoring system through
the motor. This system can be used to rotate the roller vessels during transport to                              the external jack. The alarm system is installed in the Roller Culture Apparatus.
and from the warm room or incubator. The system consists of two long-life sealed
batteries, an automatic charger system, and an independent speed control which
can be preset to maintain a bottle speed of approximately 25% of full speed dur-                                 Visual: ............................................................ 3 LED indicators flash on and off
ing the lose of the primary voltage.                                                                             Audible: ....................................................................................... Loud buzzer
Charger Specifications:                                                                                           Remote signal: ...................................................... 4 pin panel mount mic jack
Recharge time is 8 hours for discharged batteries, shorter for partially discharged                              Alarm outputs: ....... Pulsating +12VDC (25ma), Dry contact closure .5A at 24VDC
batteries. Charger automatically monitors temperature and adjusts charge voltage                                 Cat. No.       Description                                                                       Qty/Case
for correct charging levels at elevated incubator temperatures.
                                                                                                                 Factory Installed (Must be ordered with Roller Culture Apparatus)
 Cat. No.        Description                                                                      Qty/Case
                                                                                                                 349011         Rotation Alarm System Modular Cell Production Roller Apparatus                             1
 Factory Installed (Must be ordered with Roller Culture Apparatus)                                               349013         Rotation Alarm System for Cost Buster Production Roller Apparatus                          1
 349017          Battery Backup System for Modular Cell & Cost Buster Roller Apparatus                     1     Field Retrofittable Rotation Alarm System
 Field Retrofittable Battery Backup System                                                                        Same as above except packaged for field installation on Wheaton Modular & Cost Buster Production Roller
 Same as above except packaged for field installation on Wheaton Modular and Cost Buster Production Roller        Culture Apparatus which conforms to UL, CSA & CE standards.
 Culture Apparatus which conforms to UL, CSA & CE standards.                                                     349014         Rotation Alarm System for Modular Roller Culture Apparatus                                 1
 349018          Battery Backup System for Modular Cell & Cost Buster Roller Apparatus                     1     349016         Rotation Alarm system for Cost Buster Production Roller Culture Apparatus                  1




                                                                                                                                        For Incubators for your Roller Culture Apparatus see page 64.




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61


                                                               • 1 five-position base unit and up to 8 additional five position decks
                                                               • Distance between decks 7 1/8” (18.1 cm)                                              Specifications:
                                                               • Provides up to 75,000 cm2 total surface area for cell growth
                                                               • Greaseless bearings prevent leaching and eliminate bearing seizures                  Electrical: ........100 - 120VAC, 50/60Hz, 35 watts
                                                               • Optional rotation alarm and battery backup systems available                            .....................220 - 240VAC, 50/60Hz, 35 watts
                                                               • All-position drive is standard allowing the use of glass or plastic                  Bottle Speed: .....0.25 – 5.3 RPM (110 mm bottle)
                                                                 roller bottles                                                                       Operating Temperature: ....................15 to 40°C
                                                               • Conforms to UL, CSA and CE Standards
                                                               • 1 year warranty                                                                      Humidity: ................................... 80% up to 31°C
                                                               The Wheaton Modular Cell Production Roller Culture Apparatus allows                    Altitude Limit: ................................. 2000 meters
                                                               flexibility for scale-up and production of monolayer cell cultures in                   Dimensions W x D x H:
                                                               standard roller bottles. It accepts bottles from 108 to 121 mm in diameter               Base with 1 Deck: .....29.75 x 24.375 x 13.5 (in)
                                                               and up to 550 mm in length. The system consists of a base drive unit and                                             75.7 x 63.0 x 34.3 (cm)
                                                               five-position roller deck to which additional roller decks can be added.                  Base with 9 Decks: ...29.75 x 24.375 x 70.5 (in)
                                                               A powerful DC motor, with soft-start speed control, drives the bottles                                                   75.6 x 63 x 179 (cm)
                                                               through a series of durable nonslip belts.                                             Weight:
                                                                                                                                                        Base with 1 Deck: ...................... 70 lb (31.8 kg)
                                                               The all-position drive model is a positive traction drive system for                     Base with 9 Decks: ................... 238 lb (108 kg)
                                                               lightweight plastic bottles in which each roller is individually driven. Also            Additional Decks: ....................... 21 lb (19.5 kg)
                                                               included is a locking speed control knob that prevents accidental change
                                                               in rotation rates.
                                              This unit is available with one of seven standard electrical plugs shown.
     Additional configurations are available through special ordering. Contact Wheaton for more information. The
     Modular Cell Production Apparatus is supplied without roller vessels.
     Shipping Unit: One per case
     Cat. No.                Plug Style                               Voltage           Cat. No.                Plug Style            Voltage     Cat. No.            Plug Style                          Voltage
     Base with 1 Deck - 5 Bottle Positions                                              Base with 4 Decks - 20 Bottle Positions                   Base with 7 Decks - 35 Bottle Positions
     348960-A                North America                           120 VAC            348963-A                North America        120 VAC      348966-A            North America                      120 VAC
     348960-B                Japan                                   100 VAC            348963-B                Japan                100 VAC      348966-B            Japan                              100 VAC
     348970-C                Continental Europe                      230 VAC            348973-C                Continental Europe   230 VAC      348976-C            Continental Europe                 230 VAC
     348970-D                United Kingdom                          230 VAC            348973-D                United Kingdom       230 VAC      348976-D            United Kingdom                     230 VAC
     348970-F                Australia/China                         240 VAC            348973-F                Australia/China      240 VAC      348976-F            Australia/China                    240 VAC
     348970-G                Italy/Chile                             230 VAC            348973-G                Italy/Chile          230 VAC      348976-G            Italy/Chile                        230 VAC
     W348970-J               India                                   230 VAC            W348973-J               India                230 VAC      W348976-J           India                              230 VAC
     Base with 2 Decks - 10 Bottle Positions                                            Base with 5 Decks - 25 Bottle Positions                   Base with 8 Decks - 40 Bottle Positions
     348961-A                North America                           120 VAC            348964-A                North America        120 VAC      348967-A            North America                      120 VAC
     348961-B                Japan                                   100 VAC            348964-B                Japan                100 VAC      348967-B            Japan                              100 VAC
     348971-C                Continental Europe                      230 VAC            348974-C                Continental Europe   230 VAC      348977-C            Continental Europe                 230 VAC
     348971-D                United Kingdom                          230 VAC            348974-D                United Kingdom       230 VAC      348977-D            United Kingdom                     230 VAC
     348971-F                Australia/China                         240 VAC            348974-F                Australia/China      240 VAC      348977-F            Australia/China                    240 VAC
     348971-G                Italy/Chile                             230 VAC            348974-G                Italy/Chile          230 VAC      348977-G            Italy/Chile                        230 VAC
     W348971-J               India                                   230 VAC            W348974-J               India                230 VAC      W348977-J           India                              230 VAC
     Base with 3 Decks - 15 Bottle Positions                                            Base with 6 Decks - 30 Bottle Positions                   Base with 9 Decks - 45 Bottle Positions
     348962-A                North America                           120 VAC            348965-A                North America        120 VAC      348968-A            North America                      120 VAC
     348962-B                Japan                                   100 VAC            348965-B                Japan                100 VAC      348968-B            Japan                              100 VAC
     348972-C                Continental Europe                      230 VAC            348975-C                Continental Europe   230 VAC      348978-C            Continental Europe                 230 VAC
     348972-D                United Kingdom                          230 VAC            348975-D                United Kingdom       230 VAC      348978-D            United Kingdom                     230 VAC
     348972-F                Australia/China                         240 VAC            348975-F                Australia/China      240 VAC      348978-F            Australia/China                    240 VAC
     348972-G                Italy/Chile                             230 VAC            348975-G                Italy/Chile          230 VAC      348978-G            Italy/Chile                        230 VAC
     W348972-J               India                                   230 VAC            W348975-J               India                230 VAC      W348978-J           India                              230 VAC




     The 5-Position Deck allows you to add one 5-position deck to your Modular Cell
     Production system.
     Specifications:
     Weight: ............................................................................................. 21 lb (9.5 kg)
     Dimensions W x D x H: ............ 29 3/4 x 24 3/8 x 7 1/8 in (75.6 x 62.9 x 18.1 cm)
     Cat. No.                       Description                                                              Qty/Case
     348969                         5-Position Deck                                                                    1
                                                                                                                                                  For Glass Roller Bottles see page 15.



                                                                          Please note that Roller Culture Apparatus is made to order and not returnable.


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                                                                                                                                                                                                           62


                                            • Utilize from research to production
                                            • Modular; add capacity and options as your requirements change
                                            • From 5 to 55 positions per unit (holds 10 to 110 of the 850 cm2 vessels)
                                            • Displays accurate RPM by entering vessel diameter
                                            • Wide speed range brushless DC motor, 0.25 to 8.0 RPM
                                            • Programmable rotation direction, fixed or reversing                 Specifications:
                                            • Programmable soft-start and stop                                   Electrical: .................100 - 240 VAC, 50/60Hz, 20 watts
                                            • 10 program storage registers
                                            • Optional temperature monitoring, one to four sensors per unit      Bottle Dimensions Dia/L: 110 to 121 mm / up to 550 mm
                                            • Optional battery backup                                            Bottle Speed: .............. 0.25 – 8.0 RPM (110 mm bottle)
                                            • CART2 ready! Optional software for monitoring controlling,
                                              & recording up to 255 units                                        Deck Spacing: inches (cm)
                                            • Multi-language display prompts. Choice of English, French,             All Position Drive R2P .........................6 in (15.24 cm)
                                                                                                                     Modular R2P .................................7 1/8 in (18.1 cm)
                                              German, Italian, Portuguese, or Spanish
                                            • Conforms to UL, CSA and CE Laboratory Standards                    Operating Temperature: .............................. 15 to 40°C
                                            • 1 Year Warranty
                                                                                                                 Humidity: ...................... 80% up to 31°C; 50% at 40°C
                                          The Wheaton R2P Roller Culture Apparatus is designed to allow for
                                          the development and growth of cell culture from research to                         Altitude Limit: ...........................................2000 meters
                                          production quantities without having to change equipment and                        Dimensions W x D x H:
                                          revalidate protocols. This is achieved by giving you more flexible                      Base with 1 Deck: .................... 31 x 24.5 x 13.5 (in)
controls and the ability to easily add options at any time. All R2P Roller culture units share the same motor,                                                       78.8 x 62.25 x 34.3 (cm)
software, controls and controllers, which makes moving culture conditions between units fast and easy.                           Base with 3 Decks: .................. 31 x 24.5 x 25.5 (in)
All-Position Drive System                                                                                                                                            78.8 x 62.25 x 64.8 (cm)
Each roller is driven to allow for either glass or lighter-weight plastic roller culture vessels to be used.                     Base with 11 Decks: ................ 31 x 24.5 x 73.5 (in)
                                                                                                                                                                   78.8 x 62.25 x 186.7 (cm)
User-Friendly Control Panel                                                                                                      Extra Deck: ................................... 31 x 24.5 x 6 (in)
The large digital display and keypad allow for easy input and monitoring of the settings. The unit continually                                                     78.8 x 62.25 x 15.24 (cm)
displays the bottle RPM setting and the actual bottle RPM. The display remains accurate with any size bottle
(110 to 121 mm diameter) by entering the bottle diameter. The unit can be programmed to rotate in either         Weight:
                                                                                                                    Base with 1 Deck: …… .....................52 lb (23.6 kg)
direction, and can reverse direction automatically at the time interval entered (better for roller bottles fluted
                                                                                                                    Base with 3 Decks: ...........................85 lb (38.6 kg)
along the longitudinal axis). The programmable soft start and stop feature avoids premature cell detachment.        Base with 11 Decks: .......................217 lb (98.4 kg)
The settings can be saved in one of the ten memory locations for easy recall later.                                 Additional Deck: ................................16.5 lb (7.5 kg)
Built-in Monitoring and Alarms
                                                                                                                              Built-in Drive Monitoring System Alarm
The unit continuously monitors both the rotational speed and the drive train (the motor and belts). If the                        Visual: ............... Flashing display with fault displayed
speed falls out of the entered tolerance or if the unit detects a loss of rotation, the unit displays an alarm,                   Audible: ..........................Buzzer (with On/Off control)
and sounds a loud buzzer. These alarms can also be tied to an existing monitoring system through an                               External output: ........... NO or NC (programmable) dry
external jack. Optional features can be added at any time, including CART2 and battery backup.                                       contact (0.5A@ 24 VDC) through RJ-45 jack on the
                                                                                                                                     control housing front panel.
                                 6”(15.24 cm) Deck Spacing
Cat. No.            Plug Style                        Voltage                     Qty/Case
                                                                                                                                7 1/8” (18.1 cm) Deck Spacing
Base with 1 Deck - 5 Bottle Positions                                                          Cat. No.               Plug Style                              Voltage                      Qty/Case
W348880-A           North America                    120 VAC                             1
W348880-B           Japan                            100 VAC                             1     Base with 3 Decks - 15 Bottle Positions
W348880-C           Continental Europe               230 VAC                             1     W348884-A              North America                          120 VAC                                   1
W348880-D           United Kingdom                   230 VAC                             1     W348884-B              Japan                                  100 VAC                                   1
W348880-F           Australia/China                  240 VAC                             1     W348884-C              Continental Europe                     230 VAC                                   1
W348880-G           Italy/Chile                      230 VAC                             1     W348884-D              United Kingdom                         230 VAC                                   1
W348880-J           India                            230 VAC                             1     W348884-F              Australia/China                        240 VAC                                   1
                                                                                               W348884-G              Italy/Chile                            230 VAC                                   1
Base with 3 Decks - 15 Bottle Positions
                                                                                               W348884-J              India                                  230 VAC                                   1
W348881-A           North America                    120 VAC                             1
W348881-B           Japan                            100 VAC                             1     Base with 9 Decks - 45 Bottle Positions
W348881-C           Continental Europe               230 VAC                             1     W348885-A              North America                          120 VAC                                   1
W348881-D           United Kingdom                   230 VAC                             1     W348885-B              Japan                                  100 VAC                                   1
W348881-F           Australia/China                  240 VAC                             1     W348885-C              Continental Europe                     230 VAC                                   1
W348881-G           Italy/Chile                      230 VAC                             1     W348885-D              United Kingdom                         230 VAC                                   1
W348881-J           India                            230 VAC                             1     W348885-F              Australia/China                        240 VAC                                   1
                                                                                               W348885-G              Italy/Chile                            230 VAC                                   1
Base with 11 Decks - 55 Bottle Positions
                                                                                               W348885-J              India                                  230 VAC                                   1
W348882-A           North America                    120 VAC                             1
W348882-B           Japan                            100 VAC                             1     Additional Deck for Modular R2P
W348882-C           Continental Europe               230 VAC                             1     W348886                R2P 5-Position Deck, Factory-Installed (must be ordered with unit)               1
W348882-D           United Kingdom                   230 VAC                             1     W348887                R2P 5-Position Deck, for Customer Installation                                   1
W348882-F           Australia/China                  240 VAC                             1    For more information on plug styles see page 188.
W348882-G           Italy/Chile                      230 VAC                             1
W348882-J           India                            230 VAC                             1          See page 63 for the 88-Position R2P System and R2P Accessories.
Additional Deck for R2P All Position Drive
W348888             R2P 5-Position Deck, Factory-Installed (must be ordered with unit)   1
W348889             R2P 5-Position Deck, for Customer Installation                       1

                                                     Please note that Roller Culture Apparatus is made to order and not returnable.


                           WHEATON         1.800.225.1437 (U.S. & Canada only) | +1.856.825.1100 | +1.856.825.1368 (F) | www.wheatonsci.com
63




                                                                • Highest capacity roller rack
                                                                • 88 all position drive
                                                                • 1 year warranty
                                                                This unit features 6 in (15.24 cm) production spacing between the decks and holds 8 bottles/per deck.
                                                                It will not fit in standard incubators, but it will pass through standard doorways for warm rooms.
                                                                Specifications:
                                                                Dimensions W x D x H: .....................47 x 24.5 x 73.5 in (119.4 x 62.25 x 189.2 cm)
                                                                Weight: ........................................................................................ 360 lb (163.6 kg)

                                                                Cat. No.                              Plug Style                                            Voltage                        Qty/Case
                                                                Base with 11 Decks - 88 Bottle Positions
                                                                W349020-A                             North America                                        120 VAC                                1
                                                                W349020-B                             Japan                                                100 VAC                                1
                                                                W349020-C                             Continental Europe                                   230 VAC                                1
                                                                W349020-D                             United Kingdom                                       230 VAC                                1
                                                                W349020-F                             Australia/China                                      240 VAC                                1
                                                                W349020-G                             Italy/Chile                                          230 VAC                                1
                                                                W349020-J                             India                                                230 VAC                                1

                                                                                         Please note that Roller Culture Apparatus is made to order and not returnable.




                                                                                                      Network Interface is a single-line network adapter for up to 255 R2P units.
                                                                                                      Cat. No.                   Plug Style                           Voltage               Qty/Case
                                                                                                      W348902-A                  North America                        120 VAC                     1
                                                                                                      W348902-B                  Japan                                100 VAC                     1
                                                                                                      W348902-C                  Continental Europe                   230 VAC                     1
                                                                                                      W348902-D                  United Kingdom                       230 VAC                     1
                                                                                                      W348902-F                  Australia/China                      240 VAC                     1
                                                                                                      W348902-G                  Italy/Chile                          230 VAC                     1
     CART2 Screen Shot                                                                                W348902-J                  India                                230 VAC                     1



                                                                                                      • Protects cells during power outages
                                                                                                      • Provides a minimum of 18 hours of auxiliary power
                                                                                                      • Can be used to rotate vessels during transport to harvest area
                                        CART2 Screen Shot
                                                                                                      The system monitors the AC power and automatically switches to battery if AC
                                                                                                      power fails. Provides auxiliary power to maintain complete functionality of the R2P
                                                                                                      unit for a minimum of 18 hours at full capacity. The audible alarm and display
     •   Remotely monitors and controls up to 255 R2P units
                                                                                                      backlight will not function during battery power mode.
     •   Records all settings, changes, alarms, and communication loss
     •   Password-protected user levels maintain security                                             Cat. No.           Description                                                        Qty/Case
     •   Can be easily added to R2P at any time                                                       W348899            Customer Installed                                                       1
     •   Works with Windows® based software                                                           W348898            Factory Installed (Must be ordered with R2P Roller Culture Apparatus)    1
     •   Computer Requirements: IBM Pentium PC or compatible
     •   Conforms to UL, CSA and CE Laboratory Standards
     The Wheaton CART2 System precisely controls rotating speeds of up to 255                         Cat. No.           Description                                                        Qty/Case
     separate roller units. Each unit is monitored for rotation, speed deviation and
                                                                                                      W348891            Customer Installed                                                       1
     communication. This system provides the perfect solution for GMP or FDA
                                                                                                      W348890            Factory Installed (Must be ordered with R2P Roller Culture Apparatus)    1
     requirements.


     •   IBM Pentium PC or compatible
     •   32 MB of RAM, minimum
     •   Microsoft Windows® 2000, XP
     •   One free serial port
     •   10 MB of free hard drive space, minimum


                               WHEATON        1.800.225.1437 (U.S. & Canada only) | +1.856.825.1100 | +1.856.825.1368 (F) | www.wheatonsci.com
                                                                                                                                                                                                                                  64




• Designed specifically for use with Wheaton Roller Culture Apparatus                                             ELECTRICAL / ELECTRONICS:
• No lifting ramps in standard incubator. CO2 incubator has light weight ramps.                                  Requirements: ......................................................... 120-240 VAC, 1500 watts
• 40 cubic feet (1.1 m3) of space, fits:                                                                          Power Switch: .................................................................. On/Off rocker switch
      • Production-Spaced R 2P unit (6 in (15.24 cm) between decks)                                              Temperature Keypad: ................................... Digital indicator & actuator keypad
           with up to 11 decks                                                                                   AC Receptacles: ...................... Four internal (customized for voltage differences)
      • Modular-Spaced R 2P unit (7 1/8 in (18.1 cm) between decks)                                              Heating Light Heaters: .............................................................................. One
           with up to 9 decks                                                                                    Setting maintained if power is interrupted:
      • 52-Position Cost Buster
• Independent over-temperature safety protection                                                                     Circuit Breaker: ................................ None on CE Units (CE units include fuse)
• Viewing window in the door                                                                                         Failsafe Feature: Secondary mechanical high limit thermostat with indicator light
• Four internal electrical outlets for accessories                                                               TEMPERATURE:
• Can also be used for suspension cultures
• Available as standard or CO2 incubator                                                                         Heat: .................................. Forced-air circulation with digital temperature control
• Conforms to UL, CSA, and CE Standards                                                                          Temperature Range: ............................... Ambient +8 to 70°C (empty incubator)
• 1 Year Warranty                                                                                                Temperature Uniformity: .......................... to ±0.5°C @ 37°C (empty incubator)
                                                                                                                 Sensitivity:
A convenient, space-saving and economical alternative to a warm room for small                                     Standard Incubator: ...........................................................................±0.1°C
production runs. Designed to hold full-size units, it can be used in combination with                              CO2 Incubator: ...................................................................................... ±0.1°C
the optional shelves to accommodate spinner flasks, rocker tables, and other types
of equipment. Double-walled construction provides for temperature stability and easy                             DIMENSIONS:
cleaning. Shelves are not supplied with incubator.                                                               Capacity: ..............................................................................40 cubic feet (1.1 m3)
                                                                                                                 Dimensions W x D x H:
                                                                                                                    Interior: .........................................................35 x 26 x 76" (89 x 66 x 193 cm)
                                                                                                                    Exterior: ................................................ 41 x 34 x 87" (104.2 x 86.4 x 221 cm)
Specifications:                                                                                                   Weight:
Specifications are for Standard & CO2 Incubators unless specified otherwise                                           Net Weight: .............................................................................730 lb (331 kg)
                                                                                                                    Shipping Weight: ......................................................................850 lb (386 kg)
CONSTRUCTION:
External: ......................................................................................Painted Steel    Cat. No.                         Plug Style                               Voltage                   Qty/Case
Internal:
   Standard ..................................................................................Painted Steel      Standard Incubator
   CO2 ........................................................................................Stainless Steel   753680                           North America                           120 VAC                             1
Door Weight: ................................................................................ 75 lb (34 kg)      W753684-C-E                      Continental Europe                      230 VAC                             1
                                                                                                                 W753684-D-E                      United Kingdom                          230 VAC                             1
Shelf Weight Maximum: .............................................................. 80 lb (36 kg)               W753684-F-E                      Australia/China                         240 VAC                             1
Shelves Per Unit Maximum: .......................................12 Shelves (sold separately)                    W753684-G-E                      Italy/Chile                             230 VAC                             1
Recommend not blocking perforated shelf surface by more than 60% to allow adequate air flow.)                     W753684-J-E                      India                                   230 VAC                             1

Thermal Factor: ........................................................................................R13      CO2 Incubator
Accept standard cleaning chemicals                                                                               I057606                          North America                           120 VAC                             1
CO2 GAS: Gas shuts off when door is opened or switch is turned off                                               WI057606-C-E                     Continental Europe                      230 VAC                             1
                                                                                                                 WI057606-D-E                     United Kingdom                          240 VAC                             1
CO2 Connection: ........................................................1/4" (6.35 mm) hose barb                 WI057606-J-E                     India                                   230 VAC                             1
CO2 Concentration Range: .....................0-20% adjustments in 0.1% increments
                                                                                                                 For more information on plug styles see page 188.
CO2 Pressure to Indicator Inlet: ..................Rated @ 5-40 psi (0.345-2.76 bars)
                                                                not to exceed 40 psi (2.76 bars)                 Incubator Shelves (Not supplied with incubators)
CO2 Recovery time When Door is Opened and Closed:                                                                Cat. No.                         Description                                                        Qty/Case
                                                                                                                 753685                           Shelf for Standard Incubator                                                1
    10 Second Opening ..................................................................Instantaneous
                                                                                                                 WI056028                         Shelf, Stainless Steel for CO2 Incubator                                    1
    30 Second Opening ..................................................................... 10 minutes


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