Beads-driven aptamer selection Within the project called ISyPeM
Inhibition of signal lines dedicated to beads, the power line frequency noise and interference peak, but also has the ability to absorb the static pulse. Beads are used to absorb the ultra-high frequency signal, as some RF circuits, PLL, oscillator circuits, including ultra-high frequency memory circuit (DDRSDRAM, RAMBUS, etc.) need to increase the power input part of the bead, and the inductor is an energy storage Components used in the LC oscillator circuit, the low-frequency filter circuit, the frequency range of its applications are rarely more than 50MHZ. Beads have a high resistivity and magnetic permeability, equivalent series resistance and inductance, but the resistance and inductance value varies with frequency.
Beads-‐driven aptamer selection Master project for 1 student Within the project called ISyPeM (Intelligent Integrated Systems for Personalized Medicine) the CLSE Laboratory is designing a biosensor able to measure drug concentrations in biological fluids such as plasma and capillary blood samples. The ISyPeM project aims at the development of advanced technologies for assessing drug response by measuring drug concentrations, providing drug treatment indications based on processing of statistical and personal data, and enabling the seamless monitoring and delivery of drugs by integrated silicon-‐based systems. We develop and constructed a biosensor-‐chip with different electrodes size (from 1mm to 50 μm) in gold/platinum, able to detect minimal electrical currents via cyclic voltammetry. In order to sense the specific drug, we couple the electrodes with a biological interface based on DNA/RNA aptamers. Selected aptamers will recognise the drug molecules, and deliver an electrochemical signal to the electrode. We are currently exploring post-‐binding detection system based on Ferrocenium, with a signal-‐ON configuration. The aim of the Master project is to set up a pilot aptamer-‐selection method, based on Nanoparticles and Oligomere-‐Conformation Switch, in order to select and identify a DNA aptamer suitable for signal-‐OFF Ferrocenium-‐based detection of the chemotherapeutic drug Imatinib, and for the anti-‐HIV drug Efavirenz. Assessment of binding specificity and sensitivity will be performed first on SPR and then on chip. 75% Molecular biology experiments 25% Surface Plasmon Resonance tests Contact: Anna Ferretti – Fabio Spiga (email@example.com, firstname.lastname@example.org)