Diagnostic capability of CSIRO's Australian Animal Health Laboratory2011131234056

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Diagnostic capability of CSIRO's Australian Animal Health Laboratory2011131234056 Powered By Docstoc
					Diagnostic capability of CSIRO’s Australian Animal Health Laboratory
Diseases of aquatic animals
CSIRO’s Australian Animal Health Laboratory (AAHL) provides diagnostic services, enhanced surveillance and the capability to respond to
emerging and exotic aquatic animal diseases. AAHL can provide:
•    import and export testing services for a range of animals and biological products using accredited and validated tests
•    diagnosis of emergency animal diseases, including index case confirmation and national emergency response capability. Samples for exotic disease
     exclusion should be submitted by State and Territory disease control authorities
•    diagnostic testing services for surveillance programs such as the National Arbovirus Monitoring Program (NAMP) and the Northern Australia Quarantine
     Strategy (NAQS).
This list outlines AAHL's current diagnostic capability for significant diseases and other areas of expertise in relation to diseases of aquatic animals.


        Disease                             Reagents Held                     Agent Isolation or Detection             Agent                Serology             NATA
                                                                                                                   Characterisation                         Accredited Tests
Aeromonal                     A. hydrophila Type strain (ATCC 7966)     Isolation                              Biochemical            -                     -
septicaemia;                                                                                                   identification
Aeromonas hydrophila
Bacterial kidney              Renibacterium salmoninarum Type strain    Isolation                              Biochemical            -                     -
disease                       (ATCC 33209)                              Immunofluorescence                     identification
                              Exotic clinical isolates                  Antigen capture ELISA                  Sequence analysis
                              Antiserum to above strains                PCR
                              Monoclonal antibody

Bonamiosis                    Bonamia ostreae and Bonamia sp.           Histopathology                         -                      -                     -
                              infected oyster tissues as well as DNA    Electron microscopy
                              (Europe and Australia respectively)
                                                                        Detection by conventional PCR assays
Ceratomyxiasis                Ceratomyxa shasta Reference spores        Wet mounts or stained smears           -                      -                     -
                              Stains and mountants
Crustacean &                  Bacteriological or mycological media as   Histopathology                         -                      -                     -
molluscan diseases            appropriate                               Electron microscopy
                                                                        Sub-gross dissection and isolation




Diagnostic testing capability – Disease testing for aquatic animals
        Disease                             Reagents Held                       Agent Isolation or Detection                Agent               Serology        NATA
                                                                                                                        Characterisation                   Accredited Tests
Edwardsiellosis               Edwardsiella tarda                          Isolation                                 Biochemical             -              -
                              E. ictaluri                                 Immunofluorescence                        identification
Enterococcus                  Type strain (ATCC 49156)                    Isolation                                 Biochemical             -              -
seriolicida                   Enzootic and exotic isolates                Slide agglutination test                  identification
                              Antisera to enzootic and exotic strains     ELISA                                     Protein profile by
                                                                                                                    SDS-PAGE
                                                                                                                    Western blot
Epizootic                     Redfin and trout strains                    Cell culture                              Sequence analysis       -              Electron microscopy
haematopoietic                Antisera                                    Antigen capture ELISA                     Immunocytochemistry                    Virus isolation
necrosis
                                                                          Electron microscopy, Immune EM            Western blot
                                                                          Immunohistochemistry
                                                                          Immunofluorescence
                                                                          PCR
Flexibacteriosis              Flexibacter maritimus                       Isolation                                 Biochemical             -              -
                              Enzootic and exotic isolates                ELISA                                     identification
                              Antisera to enzootic and exotic strains                                               Protein profile by
                                                                                                                    SDS-PAGE
                                                                                                                    Western blot
Furunculosis / goldfish       Aeromonas salmonicida reference strain      Isolation                                 Biochemical             -              -
ulcer disease (GUD)           (ATCC 14174)                                Ag ELISA                                  identification
                              Exotic and enzootic isolates from GUD       Immunofluorescence                        Protein profile by
                              Large range of pathovars and biovars                                                  SDS-PAGE
                                                                          PCR A salmonicida sp
                              Antisera to reference strain and selected                                             Western blot
                                                                          PCR A salmonicida subsp salmonicida
                              GUD isolates                                (exotic)                                  Sequence analysis
                              Commercial monoclonal antibody
Hepatopancreatic              Commercial diagnostic kit                   Histopathology                            In situ hybridisation   -              -
parvovirus disease of
prawns                                                                    Electron microscopy

Ichthyophonus disease         -                                           Histopathology                            -                       -              -




Diagnostic testing capability – Disease testing for aquatic animals                                             September 2008
        Disease                             Reagents Held                     Agent Isolation or Detection                     Agent               Serology               NATA
                                                                                                                           Characterisation                          Accredited Tests
Infectious                    Exotic IHN isolates RB-76, LWS, SRCV,     Virus isolation                                Western blot           Serology using virus   Electron microscopy
haematopoietic                Coleman, CR, WRAC                                                                                               neutralisation tests
necrosis                                                                Electron microscopy                            Sequence analysis                             Virus isolation
                              Antiserum to above isolates
                                                                        Immunohistochemistry
Infectious hypodermal         Commercial diagnostic kit                 Histopathology                                 Sequence analysis      -                      -
and haematopoietic
necrosis (IHHN) of                                                      In situ hybridization                          Immunocytochemistry
prawns                                                                  Electron microscopy
Infectious pancreatic         IPNV strains Sp, Ab and VR299, Erwin,     Virus isolation                                Western blot           Serology using virus   Electron microscopy
necrosis                      WB, He, TV-2, Halifax ‘A’, Fish Creek,                                                                          neutralisation tests
                              81-440, Jasper, TV-2                      Electron microscopy                            Sequence analysis                             Virus isolation

                              Antisera to above                         Immunohistochemistry                           Immunocytochemistry
                                                                        PCR
Infectious salmon             Reference strains from Canada, Scotland   Histopathology                                 Sequence analysis      -                      Electron microscopy
anaemia                       and Norway
                                                                        Isolation                                      Immunocytochemistry
                              Polyclonal and monoclonal antibodies
                              from OIE Reference Laboratory             Electron microscopy
                                                                        Immunohistochemistry
                                                                        PCR
Koi herpesvirus               Reference isolates                        Histopathology                                 Sequence analysis      -                      -
disease
                              Susceptible cell lines                    Virus isolation
                                                                        Electron microscopy
                                                                        PCR
Mycobacteriosis               Mycobacteria fortuitum reference strain   Isolation and presumptive identification       -                      -                      -
                                                                        Histopathology
Nocardiosis                   Nocardia asteroids Type strain (ATCC      Isolation and presumptive identification       -                      -                      -
                              19247)
                                                                        Histopathology




Diagnostic testing capability – Disease testing for aquatic animals                                                September 2008
        Disease                              Reagents Held                      Agent Isolation or Detection                  Agent              Serology       NATA Accredited
                                                                                                                          Characterisation                          Tests
Nuclear polyhedrosis          Commercial diagnostic kits                Histopathology                                -                      -              -
baculoviroses of                                                        In situ hybridization
prawns
                                                                        Electron microscopy
Oncorhynchus masou            Type strain                               Virus isolation                               -                      -              Electron microscopy
virus disease                 Antisera                                  Electron microscopy                                                                 Virus isolation
Pasteurellosis                Pasteurella piscicida reference strain    Isolation                                     Biochemical            -              -
                              (ATCC 17911)                                                                            identification
                              Antisera to above
Piscirickettsiosis            DNA from LF-89, ATL-4-91 and Nor-92       Isolation                                     Biochemical            -              -
                              isolates (small amounts). DNA from the    Electron microscopy                           identification.
                              Tasmanian rickettsia-like organism                                                      Sequence analysis
                              (RLO). Paraffin blocks of LF-89 isolate   Detection by conventional and real-time PCR
                              infected fish tissues                     assays                                        Immunocytochemistry
                              Antiserum                                 Immunohistochemistry

Protozoan / metazoan          mountants and fixatives                   wet and stained preparations                  -                      -              -
parasitism                                                              gross/sub-gross dissection
                                                                        isolation
                                                                        Histopathology
Red sea bream                 Reference isolate                         Virus isolation                               Sequence analysis      -              -
iridoviral disease            Antiserum from OIE Reference Lab.         PCR
Spring viraemia of carp       SVCV exotic viral isolates S30, 980528,   Virus isolation                               SDS-PAGE protein       -              Electron microscopy
                              V79, F4 and 994591                        Electron microscopy                           profile                               Virus isolation
                              Antisera to SVCV                          Immunohistochemistry                          Sequence analysis
                              Monoclonal antibody                                                                     Immunocytochemistry
Streptococcosis               Streptococcus sp. isolates                -                                             -                      -              -
                              Antisera to various enzootic strains




Diagnostic testing capability – Disease testing for aquatic animals                                              September 2008
        Disease                             Reagents Held                     Agent Isolation or Detection            Agent                   Serology        NATA
                                                                                                                  Characterisation                       Accredited Tests
Taura syndrome of             TSV commercial diagnostic kit             Histopathology                           Sequence analysis        -              -
prawns
                                                                        In situ hybridization
                                                                        Electron microscopy
                                                                        Conventional PCR
                                                                        TaqMan assay
Vibriosis                     V. anguillarum Type strain (ATCC 19264)   Isolation                                Biochemical              -              -
                                                                                                                 identification
                              V. harveyi Type strain (ATCC 14126)       PCR
                               V. campbellii Type strain (ATCC 25920)                                            Computer assisted
                                                                                                                 classification
                              V. parahaemolyticus Type strain (ATCC
                              17802)                                                                             Comprehensive
                                                                                                                 identification service
                              V. alginolyticus Type strain (ATCC                                                 including marine and
                              17749)                                                                             non-halophilic
                              V. natriegens Type strain (ATCC 14048)                                             species

                              V. nereis Type strain (ATCC 25917)                                                 Sequence analysis
                              V. vulnificus Type strain (ATCC 27562)
                              V. fluvialis Type strain (NCTC 11327)
                              V. splendidus Type strain (ATCC 33125)
                              V. Iogei Type strain (ATCC 29985)
                              V. marinus Type strain (ATCC 15381)
                              V. costicola Type strain (ATCC 33508)
                              Antisera to selected serotypes of V.
                              anguillarum

Viral encephalopathy          Endemic isolates                          Histopathology                           Sequence analysis        -              -
and retinopathy
                              Positive control nucleic acid from        Virus isolation
                              Australian and exotic isolates
                                                                        Electron microscopy
                              Antisera from Oonoomba RVL
                                                                        PCR




Diagnostic testing capability – Disease testing for aquatic animals                                          September 2008
        Disease                             Reagents Held                         Agent Isolation or Detection                 Agent                   Serology                NATA
                                                                                                                           Characterisation                               Accredited Tests
Viral haemorrhagic            VHS European serotypes 1, 2, 3                Virus isolation                              Western blot              Serology using         Electron microscopy
septicaemia                                                                                                                                        virus neutralisation
                              N American isolate                            Electron microscopy                          Immunocytochemistry       tests                  Virus isolation
                              Antisera to above                             Immunohistochemistry                         Sequence analysis
                                                                            PCR
Whirling disease              Myxobolus cerebralis reference spores in      Tissue homogenisation, digestion and spore   Sequence analysis         -                      -
                              tissues                                       concentration for identification
                              Extraction and digestion buffers              PCR
White spot syndrome           WSSV exotic Thailand isolates                 Histopathology                               Sequence analysis         -                      WSSV PCR
of prawns
                              Commercial diagnostic kits                    In situ hybridization                        Immunohistochemistry
                              Polyclonal antisera                           Electron microscopy
                              Monoclonal antibody                           PCR
                                                                            Taqman assay
Yellow head disease of        YHD exotic Thailand isolates                  Histopathology                               Sequence analysis         -                      -
prawns
                              Commercial diagnostic kits                    In situ hybridization
                                                                            Electron microscopy
                                                                            PCR
Yersiniosis/ enteric          Yersinia ruckeri type strain (ATCC            Isolation                                    Biochemical               -                      -
redmouth disease              29473)                                                                                     identification
                                                                            ELISA
                              Large collection of exotic and enzootic                                                    Protein profile by SDS-
                              isolates – all recognized serotypes           PCR                                          PAGE
                              Antisera to pathogenic exotic & enzootic                                                   Western blot
                              strains
                                                                                                                         Sequence analysis


CSIRO Livestock Industries, Australian Animal Health Laboratory
Contact       AAHL Accessions Administrators

Phone         + 61 3 5227 5000                       Fax + 61 3 5227 5555               Email Aahl-Accessions@csiro.au




Diagnostic testing capability – Disease testing for aquatic animals                                                  September 2008

				
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