clinchem.2007.091157-1

							Supplemental Data Table 1: Primer sequences

Target microbial       Primer                 Sequence (5’  3’)          Amplicon
    species             name                                             length (bp)
   Neisseria          Tplaq769        ACTGGYGTTGAIATGTTCCGYAA
                                                                                245
  meningitidis        Tplaq991        ACGTCAGTIGTACGGAARTAGAA
    Listeria          Tbsi863           AACGTGGTCAAGTWTTAGC
                                                                                142
 monocytogenes        Tbsi985          GTACGGAARTAGAATTGWGG
                     ECanG459a       CAAGATGGAYTCYGTYAAITGGGA
 Candida krusei                                                                 293
                     ECanG728b       CATCTTGTAATGGTAATCTTAATG


Supplemental Data Table 2: Capture probe sequences

Target microbial       Capture probe             Sequence (5’ 3’)           Tm (°C)
    species                name
   Neisseria            TNmenH928        TACGTACTGAGCAAAGAAGA                  56.3
  meningitidis
                        TLmonH885        GTGGAGTAATCGAACCTGGT                  60.4
     Listeria           TLmonH886         TGGAGTAATCGAACCTGG                   57.6
  monocytogenes         TLmonH886a        GGAGTAATCGAACCTGG                    57.2
                        TLmonH916        AAAACATAAGTTTCAGCTTT                  50.2
                         ECkruH678       ATTAGAAGCAATCGATGCTA                  54.3
  Candida krusei         ECkruH703        GGTTGGTCTGACAGGTGG                   62.2
                         ECkruH715        TAATGGCTTTTCGGTTGG                   55.3
    Candida              ECdubH596       GTTGGTGGAAGCTICAATCA                  58.4
   dubliniensis
 Candida albicans        ECalbH596        GTTGGTGIATGGTTCAATCA                 56.3


Supplemental Data Table 3: Control oligonucleotide sequences

  Oligonucleotide name            Sequence (5’  3’)                 Tm (°C)
   bbc1 (capture probe)       AGGATAGGCAGACCATACTC
                                                                      60.4
bbc1a (Cy3-labelled target)   GAGTATGGTCTGCCTATCCT