New Integrated GC Guard Columns for Built-In Protection

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					New
Integrated GC Guard Columns
for Built-In Protection
Advantages of New                                            Zebron’s NEW Guardian eliminates traditional guard
Guardian Technology
                  TM                                         column problems because it does away with manual
                                                             installations and the need for capillary unions.
vs. Traditional Union
Connected Guard Column                                        ���������

4Complete deactivation
4Minimal affect on chromatography
4Quick set-up                                                   ����������������������������
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4Simple installation
4Out-of-box Built-in column protection




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Easy to Recognize                                                                                                      ������������������
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Guardian integrated guards are easily recognized.
Small identification labels are placed at the guard
end of every column. Besides labeling, the entire
5-meter or 10-meter guard section is left ungrouped
as a small loop of heat resistant string surrounding the
rest of the column; you will always be able to instantly
identify which end of your column is the Guardian end.

                                                                                                                        Additional 5-10 meter
                                                                                                                        Guard Column Section




                                                                                                                        Analytical Column
                                                                                                                        Section




                       PHENOMENEX • TEL: 310-212-0555 • FAX: 310-328-7768 • WWW.PHENOMENEX.COM
 GUARDIAN:
 Quick and Easy to Set-up
 With Guardian you get your column plus 5 or 10
 meters of additional integrated deactivated tubing.
 However, it’s quick and easy to replace your existing
 column with a Guardian.




 GC’s with Electronic                                                   GC’s without Electronic
 Pressure Control (EPC)                                                 Pressure Control (EPC)
 A GC with EPC controls typically asks you to manually                  Using an older system where column head pressure is
 enter your new column dimensions so that it may                        fixed and flow rate is manually calculated - at a specific
 calculate the flow rate. It is necessary to account for                 temperature - requires a slightly different adjustment.
 the additional 5 or 10 meters of guard column and                      We suggest you increase starting head pressure by
 input accordingly. For instance, if you were using a                   about 15% for 5-meter Guardian and 30% for 10-meter
 typical 30-meter column and you added a 10-meter                       Guardian columns. Next, inject a standard test mix,
 Guardian, you should enter the overall column length                   evaluate the changes in chromatography, and further
 as a 40-meter column. Next, inject a standard test                     optimize your new Guardian by increasing or decreasing
 mix, evaluate the changes in chromatography, and                       head pressure as necessary.*
 further optimize your new Guardian by increasing or
 decreasing head pressure as necessary.*


* Please  remember these are intended only as guidelines. We strongly
  recommend you actually measure linear flow rate by injection of an
  unretained peak such as methane or by using a flow meter. For more
  information on how to measure flow rate, please contact your local
  Phenomenex representative.




                           PHENOMENEX • TEL: 310-212-0555 • FAX: 310-328-7768 • WWW.PHENOMENEX.COM
Benefits of Using a
Guard Column                                                                                                                         or contain other impurities. Contaminants are trapped
4 Extends column lifetime                                                                                                            within the first 4-6 inches of the integrated guard
4 Traps non-volatile contaminants                                                                                                    section of column, thus allowing easy removal of
                                                                                                                                     contaminated guard section during normal mainte-
4 Prevents stationary phase damage
                                                                                                                                     nance procedures Since the guard section of column
4 Increases focusing for low boiling compounds
                                                                                                                                     does not contain stationary phase, separation is not
Guardian extends column lifetime when analyzing                                                                                      affected by maintenance trimming. As a result,
samples that are acidic/basic, contain inorganic salts,                                                                              column lifetime is typically increased by at least 20%.



Effect of Guard Column
on Chromatography                                                                                                                    examples below. Polyaromatic Hydrocarbon (PAH)
The guard portion of the column doesn’t significantly                                                                                 separations exhibit similar resolution without changes
effect separation or retention as is shown in the                                                                                    in retention time.
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                                                                                                               Little or No Affect
                                                                                                               on chromatography


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                         Polynuclear Aromatic Hydrocarbons (PAHs)                                                                                            Polynuclear Aromatic Hydrocarbons (PAHs)
                         Column:                 Zebron ZB-5                            Gold Seal:        Phenomenex single                                  Column:                 Zebron ZB-5 with 5 meter Guardian      Gold Seal:    Phenomenex single
     APP ID: 14872




                                                                                                                                             APP ID: 14942




                         Dimensions:             30meter x 0.25mm x 0.25µm                                groove (AG0-7518)                                  Dimensions:             30meter x 0.25mm x 0.25µm                            groove (AG0-7518)
                         Order No.:              7HG-G002-11                            Liner:            Single taper/Gooseneck                             Order No.:              7HG-G002-11-GGA                        Liner:        Single taper/Gooseneck
                         Injection:              Split 15:1 @ 260°C, 1µL                                  liner (AG0-4680)                                   Injection:              Split 15:1 @ 260°C, 1µL                              liner (AG0-4680)
                         Carrier Gas:            Constant Flow Helium 1.4mL/min         Septum:           Phenomenex                                         Carrier Gas:            Constant Flow Helium 1.4mL/min         Septum:       Phenomenex
                         Oven Program:           170°C to 240°C at 15°C/min to 275°C                      PhenoRed-400                                       Oven Program:           170°C to 240°C at 15°C/min to 275°C                  PhenoRed-400
                                                 at 4°C/min to 320°C at 10°C/min for 5 min                (AG0-4690)                                                                 at 4°C/min to 320°C at 10°C/min for 5 min            (AG0-4690)
                         Detector:               MSD@240°C                                                                                                   Detector:               MSD; 50-450amu
                         Sample                                                                                                                              Sample
                         1. Naphthalene               10.        Chrysene                                                                                    1. Naphthalene               10.    Chrysene
                         2. Acenaphthene              11.        Benzo[b]fluoranthene                                                                         2. Acenaphthene              11.    Benzo[b]fluoranthene
                         3. Acenaphthylene            12.        Benzo[k]fluoranthene                                                                         3. Acenaphthylene            12.    Benzo[k]fluoranthene
                         4. Fluorene                  13.        Benzo[a]pyrene                                                                              4. Fluorene                  13.    Benzo[a]pyrene
                         5. Phenanthrene              14.        Indeno[1,2,3-cd]pyrene                                                                      5. Phenanthrene              14.    Indeno[1,2,3-cd]pyrene
                         6. Anthracene                15.        Dibenz[a,h]anthracene                                                                       6. Anthracene                15.    Dibenz[a,h]anthracene
                         7. Fluoranthene              16.        Benzo[g,h,i]perylene                 Notes: All analytes                                    7. Fluoranthene              16.    Benzo[g,h,i]perylene             Notes: All analytes
                         8. Pyrene                                                                    are 100µg/mL in                                        8. Pyrene                                                            are 100µg/mL in
                         9. Benz[a]anthracene                                                         methylene chloride.                                    9. Benz[a]anthracene                                                 methylene chloride.




Should you be using a
guard column?                                                                                                                        �
                                                                                                                                     �
If you are working with samples that contain unknown                                                                                 � Prevent acidic and/or basic matrix compounds
                                                                                                                                     2
impurities, chances are you should be using a guard.                                                                                 � from stripping the stationary phase
Guard columns help protect your expensive analytical                                                                                 �
                                                                                                                                     � Reduces retention time shifts due to column
                                                                                                                                     3
column by:
                                                                                                                                       maintenance.
� Trapping non-volatiles that would otherwise
1
                                                                                                                                     Bottom line, if you want longer column life, you
  deposit on the column
�                                                                                                                                    should be using a guard column!
�
                                                            PHENOMENEX • TEL: 310-212-0555 • FAX: 310-328-7768 • WWW.PHENOMENEX.COM
                EPA Method 8270C:
                Semivolatiles
                Column:              Zebron ZB-5 with 5 meter Guardian
APP ID: 14945




                Dimensions:          30meter x 0.25mm x 0.25µm
                Order No.:           7HG-G002-11-GGA
                Injection:           Split 10:1 @ 250°C, 1µL
                Carrier Gas:         Constant Flow Helium 0.9 mL/min
                Oven Program:        50°C for 2 min to 70°C
                                     at 20°C/min to 195°C
                                     at 16°C/min to 285°C
                                     at 30°C/min to 320°C
                                     at 6°C/min for 4min
                Detector:            MSD; 630-450 amu




                                                                                                                                   3                   5               7               9                      11                 13              15                 17              19              21 min
                                                                                                                         18-20


                                                                                                                          21-22                        36,37
                                                                                                                                                    35
                                                                                                                                       29         34
                                                                                              12 13 15 16                 23             32                  39
                                                                                                   14                             27   31 33               38
                                                                                          9 11                            24
                                                                                         8 10           17                   25 26 28
                                                 3                  6         7                                                       30
                            1 2                            5
                                                      4

                 3                       4                     5                        6                            7                      8                     9

                                                                                                                     69,70                         84,85                                                                        103,104

                                                                                                                                                  83                                             90 92
                                                                                56,57                                    74                                                       86
                                                                                            59                                                                                                               96           102
                                                                                                        66,67                              80                                                                       98
                                                                                                 62             68                77,78 79                                                  89                    97                                    107
                                                                                                                                                                                                                                                                                         110,111
                                              44 45            51                                                       75                                                                                                                           106
                                                                          54,55                       63                                          82                                                     95               101
                                         43           46 48                                                               72                    81                                                91                   100                105                 108                                  112
                            40,41                                        53                            64
                                    42                 47 49 50     52                                  65           71    73
                                                                                                                                                                                                                     99
                                                                                        58     61                                                                                                       94                                                               109
                                                                                                                                  76                                                   87              93
                                                                                             60
                                                                                                                                                                                        88

                  8.5                          9.5                       10.5                           11.5                           12.5                                 13              14                    15             16             17             18              19        20              21




                Sample Analyte                                                           Sample Analyte                                                               Sample Analyte                                                       Sample Analyte

                 1.     N-Nitrosodimethylamine                                           29.        2,4-Dimethylphenol                                                57.   Acenaphthylene                                                  85.        Anthracene
                 2.     Pyridine                                                         30.        Benzoic Acid                                                      58.   2-Nitroaniline                                                  86.        Di-n-butylphthalate
                 3.     2-Picoline                                                       31.        Bis(2-chloroethoxy)methane                                        59.   Acenaphthene                                                    87.        4-Nitroquinoline-n-oxide
                 4.     N-Nitrosomethylethylamine                                        32.        2,4-dichlorophenol                                                60.   2,4-Dinitrophenol                                               88.        Methapyrilene
                 5.     Methyl methanesulfonate                                          33.        µ,µ-Dimethylphenylamine                                           61.   4-Nitrophenol                                                   89.        Isodrin
                 6.     N-Nitrosodiethylamine                                            34.        1,2,4-Trichlorobenzene                                            62.   Dibenzofuran                                                    90.        Fluoranthene
                 7.     Ethyl Methanesulfonate                                           35.        Naphthalene                                                       63.   Pentachlorobenzene                                              91.        Benzidine
                 8.     Phenol                                                           36.        4-Chloroaniline                                                   64.   2,4-Dinitrotoluene                                              92.        Pyrene
                 9.     Aniline                                                          37.        2,6-Dichlorophenol                                                65.   1-Naphthalenamine                                               93.        Aramite (isomer)
                10.     Bis(2-chloroethyl)ether                                          38.        Hexachloropropene                                                 66.   2-Naphthalenamine                                               94.        Aramite (isomer)
                11.     2-Chlorophenol                                                   39.        Hexachloro-1,3-Butadiene                                          67.   2,3,4,6-Tetrachlorophenol                                       95.        Dimethylaminoazobenzene
                12.     1,3-Dichlorobenzene                                              40.        1,4-Phenylenediamine                                              68.   Diethyl Phthalate                                               96.        Chlorobenzilate
                13.     1,4-Dichlorobenzene                                              41.        N-Nitrosodibutylamine                                             69.   Fluorene                                                        97.        3,3-Dimethylbenzidine
                14.     Benzyl Alcohol                                                   42.        4-Chloro-3-methylphenol                                           70.   4-Chlorophenyl phenyl ether                                     98.        Benzyl butyl phthalate
                15.     1,2-Dichlorobenzene                                              43.        Safrole                                                           71.   2-Methyl-5-nitroaniline                                         99.        Kepone
                16.     2- Methylphenol                                                  44.        1-Methylnaphthalene                                               72.   4-Nitroaniline                                                 100.        2-Acetylaminofluorene
                17.     Bis(chloroisopropyl)ether                                        45.        2-Methylnaphthalene                                               73.   2-Methyl-4,6-dinitrophenol                                     101.        3,3’-Dichlorobenzidine
                18.     4-Methylphenol; 3-Methylphenol                                   46.        1,2,4,5-Tetrachlorobenzene                                        74.   Diphenlamine                                                   102.        Benz[a]anthracene
                19.     N-Nitrosopyrrolidine                                             47.        Hexachlorocyclopentadiene                                         75.   Azobenzene                                                     103.        Bis(2-ethylhexyl)phthalate
                20.     Acetophenone                                                     48.        2,4,6-Trichlorophenol                                             76.   1,3,5-Trinitrobenzene                                          104.        Chrysene
                21.     N-Nitrosomorpholine                                              49.        2,4,5-Trichlorophenol                                             77.   Phenacetin                                                     105.        Di-n-octyl phthalate
                22.     N-Nitroso-di-n-propylamine                                       50.        Isosafrole (isomer)                                               78.   4-Bromophenyl phenyl ether                                     106.        Benzo[b]fluoranthene
                23.     2-Toluidine                                                      51.        2-Chloronaphthalene                                               79.   Hexachlorobenzene                                              107.        Benzo[k]fluoranthene
                24.     Hexachloroethane                                                 52.        3-Nitroaniline                                                    80.   4-Aminobiphenyl                                                108.        Benzo[a]pyrene
                25.     Nitrobenzene                                                     53.        1,4-Naphthoquinone                                                81.   Pentachlorophenol                                              109.        3-Methylcholanthrene
                26.     N-Nitrosopiperidine                                              54.        Dimethyl phthalate                                                82.   Pentachloronitrobenzene                                        110.        Indeno[1,2,3-cd]pyrene
                27.     Isophorone                                                       55.        1,3-Dinitrobenzene                                                83.   Phenanthrene                                                   111.        Dibenz[a,h]anthracene
                28.     2-Nitrophenol                                                    56.        2,6-Dinitrotoluene                                                84.   Dinoseb                                                        112.        Benzo[g,h,i]perylene



                                                                          PHENOMENEX • TEL: 310-212-0555 • FAX: 310-328-7768 • WWW.PHENOMENEX.COM
411 Madrid Avenue
                                                                                                                                                                                    PRSRT STD
Torrance, CA 90501-1430
                                                                                                                                                                                  U.S. POSTAGE
U.S.A.
                                                                                                                                                                                       PAID
                                                                                                                                                                                   PERMIT #185
                                                                                                                                                                                  TORRANCE, CA




                                                                                                                                 If Zebron does not provide you with equivalent
                                                                                                                                 separations as compared to any other GC column
                                                                                                                                 of the same phase and comparable dimensions,
                                                                                                                                 send in your comparative data within 45 days
                                                                                                                                 and keep the Zebron column for FREE!


          Ordering Information
          GC Column Phase            Dimensions                              5m GuardianTM Order No.                     Price           10m GuardianTM Order No.                  Price
          ZB-1ms
          Zebron ZB-1ms               30 meter x 0.25 mm x 0.25 df(μm)        7HG-G011-11-GGA                                             7HG-G011-11-GGC
          ZB-5ms
          Zebron ZB-5ms               15 meter x 0.25 mm x 0.25 df(μm)                 —                                                  7EG-G010-11-GGC
          Zebron ZB-5ms               30 meter x 0.25 mm x 0.25 df(μm)        7HG-G010-11-GGA                                             7HG-G010-11-GGC
          Zebron ZB-5ms               30 meter x 0.25 mm x 0.50 df(μm)        7HG-G010-17-GGA                                                       —
          Zebron ZB-5ms               30 meter x 0.25 mm x 1.00 df(μm)                 —                                                  7HG-G010-22-GGC
          Zebron ZB-5ms               30 meter x 0.32 mm x 1.00 df(μm)        7HM-G010-22-GGA                                                       —
          ZB-5
          Zebron ZB-5                 30 meter x 0.25 mm x 0.25 df(μm)        7HG-G002-11-GGA                                             7HG-G002-11-GGC
          Zebron ZB-5                 30 meter x 0.25 mm x 0.50 df(μm)        7HG-G002-17-GGA                                             7HG-G002-17-GGC
                                                                                                                                                                                                         3320_U




          Zebron ZB-5                 60 meter x 0.25 mm x 0.25 df(μm)        7KG-G002-11-GGA                                                       —
          Zebron ZB-5                 60 meter x 0.25 mm x 0.50 df(μm)        7KG-G002-17-GGA                                                       —




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