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									Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3   1
Version 001 11/01/2011




                     ……………………….…

                     CODE OF PRACTICE
                       for the use of the

            PATHOGEN IMAGING FACILITY

              UNIVERSITY OF EDINBURGH
              ASHWORTH LABORATORIES,
                  KING’S BUILDINGS




                                     Last updated: 26th September 2010
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3    2
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                                 Contents



Section                                                               Page

Main             Locale, Management, Type of Work, General                 3
                 arrangements
User Guide       FlowChart                                                 8
Form 1           Biosafety questionnaire for Flow Cytometry                9
                 analysis
Appendix 1       Standard Operating procedures I (non                   10
                 hazardous samples
Appendix 2       Training Record I (List of authorized users for        11
                 non-hazardous samples)
Appendix 3       Standard Operating procedures II (samples              12
                 requiring CL2)
Appendix 4a      Training Record II (for handling samples               13
                 requiring CL2)
Appendix 4b      List of authorized users for work requiring CL2        14
Appendix 5       Standard Operating procedures III (samples             15
                 requiring CL3)
Appendix 6a      Training Record III (for handling samples              17
                 requiring CL3)
Appendix 6b      List of authorized users for work requiring CL3        18
Appendix 7       CL3 work Decommissioning check list                    19
Appendix 8       List of standard microscope operating                  20
                 instructions.
Appendix 9       Charges                                                21
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3                     3
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LOCATION


The Room number of the Facility is B.64 (Ashworth 3, Basement room 64).


MANAGEMENT

This is a central shared facility for the School of Biological Sciences and the Centre for
Infectious Disease (CID) which also may be used by researcher’s outwith SBS and UoE.
The Head of School has delegated overall responsibility for the facility to an assigned
Responsible Principal Investigator (PI). Day to day management and operation of the
Confocal Microscopy Facility is under the responsibility of the Facility Manager. Advice
on safety matters may be obtained from the Biological Safety Advisor of the Ashworth
Laboratories or the School Biological Safety Officer/Safety Adviser.

At the present time the respective staff are:

Function                    Name                             Contact
Facility Manager            Dr Dianne Murray                 51 3658
Responsible PI              Prof Keith Mathews               51 3639
Ashworth Safety Adviser     Dr David Cavanagh                50 5459
School Safety Adviser       N.N.                             50 7746

Maintenance Procedures
Maintenance procedures for all major pieces of equipment are detailed elsewhere and are
the sole responsibility of the Facility Manager or the Responsible PI. Any problems with
any equipment or the operation of the Facility room should be reported to them.
It is the responsibility of the Facility Manager or the Responsible PI to issue and sign
permits of work and release certificates for equipment should this become necessary (cf.
http://www.safety.ed.ac.uk/resources/General/Lab_permit_to_work.pdf ). Users must
not allow maintenance or repair staff (contractors or University staff) to access the
facility unless they have a permit to work issued by the Facility Manager or the
Responsible PI.

Cleaners will be instructed by the Facility Manager, who will organise cleaning and
general disinfection of the facility. Guidance for cleaners is available from the safety
website and will be communicated to them by the Facility Manager before they are
given             access              to          the            facility           (c.f.
http://www.safety.ed.ac.uk/resources/Bio/Guidance/CL/Cleaning_Labs.shtm ).
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TYPE OF WORK
The work carried out in this laboratory is general and confocal microscopical analysis of
fixed and live samples. Images will be acquired and data processed for export. Fixed
samples analysed typically require only containment level 1 precautions (CL1).
However, live samples may contain pathogens in hazard group 2 (HG2) and some
samples from human sources may potentially contain pathogens including blood borne
viruses in HG3 but determined by risk assessment to be low risk for the presence of
these pathogens and for these CL2 precautions are required. Therefore, the facility will
routinely be operated observing CL2 precautions.
 Occasionally, use of the facility will be dedicated to samples known to contain
particular pathogens in hazard group 3 (mainly parasites) which can be handled at CL3
with certain derogations Any of these samples could contain materials that have been
genetically modified..
The facility is not suitable for handling other high risk samples requiring full CL3
precautions.
All users must comply with this Code of Practice (CoP) and take action as indicated in
the quick User Guide (p. 7) depending on the nature of their work (i.e. depending on
whether their work demands CL2 or derogated CL3).

Anyone wishing to use the facility must first consult with the Facility Manager (or
responsible PI), complete a Biosafety Questionnaire (Form 1; serves to register all
projects for future reference and to guarantee appropriate training) and obtain
authorization.

Risk assessment
Principal Investigators are responsible for ensuring appropriate risk assessments are
carried out for all work with the materials involved prior to sample submission to the
Facility. These will form the basis for completing Form 1. Where the samples include
materials that have been genetically modified the GM risk assessment must be
approved by the School GM Safety Committee in advance of any work proceeding.


GENERAL ARRANGEMENTS


Access
Access to the Facility is restricted to authorised users and visitors who will be given a
key to be returned to the Facility Manager after completion of work. An authorised user
must accompany visitors at all times. First time users need approval from their
respective PI and must contact the Facility Manager before using the facility. Continued
use of the Facility is subject to approval of the Responsible PI and the Facility Manager.
The Responsible PI or Facility Manager may, without notice, stop all or any work he
considers not being performed according to this CoP, as this may present a health and
safety risk to other users.
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Booking procedures:
The Facility will essentially operate on a first come first served scheme. Bookings will
initially be recorded via email by the Facility Manager or responsible PI and displayed
on the facility website http://www.biology.ed.ac.uk/research/groups/aebischer/confocal/
for general viewing. Please, think of letting the next ones know if you run late or if you
do not need all the time that you booked. Live samples analysed at 37 °C require 3h
equilibration of the system.


Machine                        Booking system                 Information to be given
Leica SP5 live                 via Email for new users        Name, phone number, time
                                                              (start/end),  nature    of
                               online for registered users    samples analysed.

Data storage / transfer:

As a multi-user facility the PC used for microscope control and image acquisition and
initial processing will not be equipped for longer term or permanent data storage. Users
are asked to export acquired data after each session via Ethernet, transportable hard disks
or other mobile data storage systems.
System hard disks will be cleared on a routine basis to prevent data overflow and hard
disk clogging.

Acknowledgement of support:

The current instrument Leica SP5 live has been bought with an equipment grant
form the BBSRC. Publications that include data acquired on this instrument
should acknowledge this support referring to the grant number BB/E012442/1 and
be made known to Prof. Keith Matthews as reference will have to be made in reporting
on the grants outcome.
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User qualification
As different users will be working with samples requiring different Containment
Levels, training will depend on the respective requirements. Please, consult the User
Guide (FlowChart, p7) for a quick reference what your needs may be. If in doubt,
please contact the Facility Manager or Responsible PI.

   Note: The Standard Operating Procedures (SOP) referred to below will not cover
         Cytofluorimeter settings or data recording software procedures
         (proficiency in these is assumed) but are regulating sample handling within
         the facility, disinfection procedures and waste disposal.

   1. All new users must demonstrate familiarity with the SOP in place for working
      with non-hazardous samples (SOP I; Appendix 1) to the Facility Manager.
      New users must make themselves known to the Facility Manager before using
      the facility. Awareness of this CoP and training shall be acknowledged by
      signing the Approved User List (Appendix 2), a Biosafety Questionnaire needs
      to be filed to register the project. This will qualify users to work with non-
      hazardous samples.
   2. Users who wish to perform analyses that require working under CL2 conditions
      must be trained and must demonstrate their competence and compliance with the
      respective SOP in place for this type of work (SOP II; Appendix 3) to the
      Facility Manager. User names will be listed on a CL2 authorized user list
      (Appendix 4b). Work can only proceed if the Biosafety Questionnaire (Form
      1) detailing the work is filled in by the Principal Investigator and accepted by
      the Facility Manager.
   3. Users who wish to perform analyses or sorting of samples that require
      working under CL3 (derogated) conditions must be trained and must
      demonstrate their competence and compliance with the respective SOPs in
      place for this type of work (SOP III; Appendix 5) to the Facility manager.
      Such users of the facility must also be an authorised user of a CL3 laboratory
      having satisfactorily completed training to work unsupervised at CL3.
      Records will be kept of staff training (Appendix 6a) and their name will be
      listed on a CL3 authorized user list (Appendix 6b). Work can only proceed if
      the Biosafety Questionnaire (Form 1) detailing the work is filled in by the
      Principal Investigator and accepted by the Facility Manager who will send a
      copy of the Form to the Ashworth Biological Safety Adviser.

   Records of training (Appendix 4a) will be kept on file by the Facility Manager.

If at anytime anyone working in the Facility fails to comply with this Code of Practice
for any reason, or in the opinion of Responsible PI, the Facility Manager or their own
Principal Investigator is not operating in an acceptable manner, they will be suspended
as authorised user, and will need to undergo further training. During this time the
suspended person is not allowed to work in the Facility unless supervised by an
authorised user.

Out of Hours Working

Working in the Facility out of normal working hours [8am - 8pm] is subject to the
approval of the user’s Principal Investigator and the Facility Manager. Whenever
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3                       7
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possible a contact, (colleague, family or friend for example), should be told of an
expected completion time for the work, (and be updated if necessary), in order that they
can take action if the worker fails to meet or contact them at the expected time.
Consideration should be given to the School’s arrangements for lone and out of hours
working                                                                             (see
http://safety.biology.ed.ac.uk/Health&Safetydocs/Safety%20Policy%20Sections/12_Sup
ervision.doc)


Disinfection
Appropriate disinfection procedures are defined in the SOPs for work under CL1, CL2
and derogated CL3 conditions, respectively. Machines are cleaned and disinfected as
part of the standard start up and close down procedures as indicated in the User manuals.
Virkon is routinely used for disinfecting surfaces such as benches after any spillage.

Waste disposal
Non-hazardous solid waste can be disposed in the designated bins in the Facility. Non-
hazardous liquid waste fluid may be poured down the sink with copious amounts of
water. Glass material (slides or cover slips) containing non-hazardous samples may be
discarded in the provided sharps bins.
All hazardous waste has to be removed and transported back to the laboratories where
the samples were prepared and where appropriate disinfection and disposal rules are in
place (see SOP II and III for further details).

Accidents
First aid:
    - In the event of an accident resulting in a wound immediately but gently encourage
    it to bleed, wash thoroughly with soap and water but DO NOT SCRUB, cover with
    waterproof dressing.
    - In the event of contamination of skin, conjunctivae or mucous membrane
    immediately wash thoroughly with copious amounts of water.
As in general within the Ashworth Laboratories, all accidents, incidents and near misses
should be reported to the person responsible for the area which in this case is the Facility
Manager and to the Ashworth Superintendent who will forward a report to the
University Health and Safety Department (see accident procedures flow chart under
http://www.safety.ed.ac.uk/resources/Bio/Guidance/General/Accident%20Procedures%2
0flow%20chart.pdf). The University Occupational Health Unit should also be informed
in the event of any accident where exposure to a pathogen or infectious material may
have occurred. A full accident record should be prepared and forwarded to the Health
and Safety Department as soon as possible. The source of any contamination (specimen,
sample, material etc) should be clearly identified and retained for testing if necessary.
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          Quick reference for users intending to analyse samples in the shared
          Confocal Microscopy Facility (B64;Ashworth3 Laboratories)


                  New?          Yes Training I                Record I
                                       Intro and SOP I                           Work with HG 1
                                                              Acknowledgement
                                       Complete Biosafety                           samples
                                       questionnaire          and approval of
                                       Contact:               CoP and SOP I
              No                       Facility manager


                                Yes Training II               Record II
               Samples                                        Acknowledged and   Work with HG 2
                                        SOP II                                      samples
               require                  Complete Biosafety    approved SOP II
                CL2?                    questionnaire         competence
                                        Contact:
              No                        Facility manager



                                Yes Training III              Record III
                                                                                 Work with HG 3
                Samples                 SOP III               Acknowledged and
                                        Complete Biosafety                          samples
                require                                       approved SOP III
                                        questionnaire
                 CL3?                   Contact:              competence
                                        Facility manager
            CL     =   Containment level
            HG     =   Hazard Group
            CoP    =   Code of practice
            SOP    =   Standard operating procedure
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3                                   9
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                                                                                            FORM 1
                        Biosafety Questionnaire for
              Shared Confocal Microscopy Facility Ashworth 3,

The Confocal Microscopy Facility will be operated as a multi-user facility where many different
samples from various sources that may contain known or unknown human pathogens are investigated
live. The safety of the staff and of users of the facility is of ultimate concern. Information about the
sample sources and potentially infectious agents is critical for effective biosafety measures.
Consequently, this sample information form must be filled out completely and signed by PI who is
requesting samples to be analyzed in the shared facility before experiments or projects are started. The
same biosafety questionnaire will be kept on file, the PI must inform the Facility Manager if any of the
information it contains changes. Appropriate risk assessment, and where necessary approval, of
experiments prior to sample submission to the core laboratory is required and the responsibility of the
User’s PI .
NOTE: Work performed in the facility on the Leica SP5 live microscope that is going to be
         published should acknowledge the support by BBSRC Grant BB/E012442/1

                                                                     Date _____________________
                Principal Investigator                 Researcher requesting access to facility
Name:

Phone
number
E-mail
Laboratory
 PROJECT INFORMATION
 Project title:

Summary or short
description of project.




Type of sample and
source
SAFETY INFORMATION
Nature of infectious agents (if known)                    Hazard Group         COSHH BA1 RA Nr.




If agent is genetically modified:
Nature of GMO                                             Hazard Group         GM RA Nr.




BILLING INFORMATION
Institute / Group / Grant                                 R-Number           Cost Centre


                                                          _____________________________
                                                           Signature (Principal Investigator)



Accepted by Facility Manager:                             _____________________________
                                                          Date and Signature
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3                    10
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                                                                           APPENDIX 1

STANDARD OPERATING PROCEDURE I

RULES FOR MICROSCOPICAL ANALYSES OF Non-hazardous SAMPLES
(A copy of these rules is to be displayed in the Facility).

   1. Projects need to be registered by filing the Biosafety Questionnaire (Form 1)
      detailing the work is filled in by the Principal Investigator and accepted by the
      Facility Manager

   2. Eating, chewing, drinking, smoking, applying cosmetics, storing of food and
      outdoor clothing in the Facility is banned.

   3. Mouth pipetting must not be used under any circumstances for any reason.

   4. All workers in the Facility must cover cuts and abrasions with a waterproof
      dressing before entering the laboratory.

   5. Laboratory coats must be worn at all times whilst in the Facility.

   6. Keyboards/mouse used to operate the computers must not be touched with
      gloves.

   7. Door handles must not be touched with gloved hands

   8. Always wash hands before leaving the Facility.

   9. Follow starting up and closing down procedures as indicated for the machines
      used (see User manuals). In particular, the cleansing routine for the objectives
      has to be followed.

   10. Solid waste (TC plastic, glass slides, etc. ) may be disposed in the available bins
       in the Facility or transported back to the home laboratory.

   11. Any non hazardous waste fluid should be emptied down the sink with copious
       amounts of water.

   12. On completion of their work each user must leave the area they have used clean
       and tidy. All waste and samples etc should be disposed of or removed and
       surfaces and work place must be disinfected with.
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3        11
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                                                     APPENDIX 2 page _____

CONFOCAL MICROSCOPY FACILITY ROOM B64 (ASHWORTH 3
BASEMENT)AUTHORISED USERS FOR WORK INVOLVING NON-
HAZARDOUS SAMPLES
The Undersigned acknowledge the rules of the CoP Confocal Microscopy Facility,
Ashworth 3 and have demonstrated competence for SOP I of the CoP to the signed
authorized user

Date     User name/Email               Authorized by Name
         Signature                                   Signature
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                                                                         APPENDIX 3
STANDARD OPERATING PROCEDURE II

RULES FOR MICROSCOPICAL ANALYSES OF LIVE SAMPLES
REQUIRING CONTAINMENT LEVEL 2 (CL2)
(A copy of these rules is to be displayed in the Facility).
   1 Work can only proceed if the Biosafety Questionnaire (Form 1) detailing the
      work is filled in by the Principal Investigator and accepted by the Facility
      Manager.
   2 Eating, chewing, drinking, smoking, applying cosmetics, storing of food and
      outdoor clothing in the Facility is banned.
   3 Mouth pipetting must not be used under any circumstances for any reason.
   4 All workers in the laboratory must cover cuts and abrasions with a waterproof
      dressing before entering the Facility.
   5 Laboratory coats must be worn at all times whilst in the Facility.
   6 Door handles must not be touched with gloved hands
   7 Always wash hands before leaving the Facility.
   8 Samples have to be transported to and from the facility in a secondary container
      with a secure lid.
   9 Gloves must be worn for all manipulations of the sample Containers holding
      potentially infective material. Gloves must not be worn when operating
      computers or control panels. (i.e. the hand changing samples on the sample bay
      is gloved; the hand operating computers is not).

   10 Waste fluid if generated has to be collected in appropriate containers to be
      removed from the facility after completion of the work.
   11 Follow start up and closing down procedures as indicated for the machines used
      (see User manuals and machine operating procedures). In particular, the
      cleansing routine for the objectives has to be followed.
   12 All waste material (plastic containers from the samples, glass slides, gloves worn
      when manipulating samples, wipe down paper) must be RETURNED to the
      home laboratory observing contained transport rules and disposed of as defined
      under the local waste disposal rules.

   13 On completion of their work each user must leave the area they have used clean
      and tidy and disinfect surfaces where samples were handled with 1% Virkon.

   14 Spills should they occur must be immediately disinfected with Virkon granules.
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3           13
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                                                                    APPENDIX 4a

DOCUMENTATION OF TRAINING FOR CONFOCAL MICROSCOPY
FACILITY WITH SAMPLES REQUIRING CONTAINMENT LEVEL 2

Trainee: ………………………………………….
Date of commencement of training: ……………..
Supervisor: ……………………………………….


     Procedure                               Date demonstrated by      Date of satisfactory
                                             training supervisor       practice
a    Transporting samples


c    Analyzing samples with gloves on
     and operating computer
     keyboard/mouse with gloves off.
d    Removal of contaminated waste ,
     disinfection of instrument and work
     place.
e    Safe transport and disposal of waste
     at the end of the work session.
f    Procedures to be adopted in the event
     of a spillage
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3        14
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                                                 APPENDIX 4b page________

CONFOCAL MICROSCOPY             FACILITY ROOM B64 (ASHWORTH 3
BASEMENT) - AUTHORISED USERS FOR WORK INVOLVING
CONTAINMENT LEVEL 2 SAMPLES
The Undersigned acknowledge the rules of the CoP Confocal Microscopy Facility,
Ashworth 3 and have demonstrated competence for SOP II of the CoP to the
signed authorized user

Date     User name/Email               Authorized by: Name
         Signature                                   Signature
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3                    15
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                                                                          APPENDIX 5
STANDARD OPERATING PROCEDURE III

RULES FOR ANALYSES OF LIVE SAMPLES REQUIRING CONTAINMENT
LEVEL 3 (CL3)

   1   Work can only proceed if the Biosafety Questionnaire (Form 1) detailing the
       work is filled in by the Principal Investigator and accepted by the Facility
       Manager Work under derogated CL3 must be arranged in advance with the
       Facility Manager in order that Facility can be dedicated to that use. Note that the
       manager will have to give notice to other users in due time (~ 2 weeks in
       advance) by email, electronic calendar and door notice.

   2   The Facility must be brought up to CL3 (derogated) standards (only
       together and in agreement with Facility Manager):

          a. Access to the Facility must be restricted by locking the door.
          b. Door signs must be fixed to indicate CL3 work (with derogations).
          c. Only users trained and authorized to perform CL3 work are allowed
             access and use of the Facility is limited to CL3 work for the time
             required.
          d. Dedicated laboratory coats must be worn at all times whilst in the Facility
             and removed before leaving for any reason.

   3   Eating, chewing, drinking, smoking, applying cosmetics, storing of food and
       outdoor clothing in the Facility is banned.

   4   Mouth pipetting must not be used under any circumstances for any reason.

   5   All workers in the Facility must cover cuts and abrasions with a waterproof
       dressing before entering the laboratory.

   6   The use of sharps is banned in the Facility.

   7   Samples have to be transported to the facility in a secondary container with a
       secure lid appropriate for CL3 materials.

   8  Gloves must be worn for all manipulations of the sample containers holding
      infective material. Gloves must not be worn when operating computers or
      control panels.
   9 Door handles must not be touched with gloved hands.
   10 Always wash hands before leaving the Facility
   11 Waste fluid if generated has to be collected in appropriate containers to be
      removed from the facility after completion of the work.
   12 Follow start up and closing down procedures as indicated for the machines used
      (see User manuals and machine operating procedures). In particular, the
      cleansing routine for the objectives has to be followed.
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   13 On completion of the work the Facility must be thoroughly cleaned and
      disinfected. Surfaces and work place (including the floor), must be disinfected
      with 1% Virkon (in particular area of the sampler bays).

   14 All waste material from the samples (containers, gloves worn when handling,
      wipe down paper) including waste fluid and the laboratory coats must be
      bagged and RETURNED to the home CL3 (derogated) laboratory and disposed
      of as defined under the local waste disposal rules.

   15 Spills must be immediately disinfected with Virkon granules.

   16 After removal of all specific waste and disinfection of all working surfaces, the
      laboratory is returned to normal operating standard:

          a. Door signs are removed.
          b. A decommissioning checklist (Appendix 7) MUST be completed.
          c. Access restrictions are brought back to normal operating level.
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                                                                       APPENDIX 6a

DOCUMENTATION OF TRAINING FOR CONFOCAL MICROSCOPY
WITH SAMPLES REQUIRING CONTAINMENT LEVEL 3

Trainee: ………………………………………….
Date of commencement of training: ……………..
Supervisor: ……………………………………….

     Procedure                             Date demonstrated      Date of satisfactory
                                           by training            practice
                                           supervisor
a    Preparation of Facility for
     temporary work under CL3
b    Transporting samples

c    preparing machine (preparation
     and installing of dedicated waste
     collectors )
d    Analyzing samples with gloves on                             :
     and operating computer
     keyboard/mouse with gloves off.
e    Removal of waste , disinfection of
     instrument and work place*.

f    Safe transport and disposal of
     waste at the end of the work
     session.
g    Procedures to be adopted in the
     event of a spillage


This training record is solely for shared Confocal Microscopy Facility. Users of the
Facility must also be an authorised user of a CL3 laboratory having satisfactorily
completed training to work unsupervised at CL3.
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3        18
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                                              APPENDIX 6b page___________

CONFOCAL MICROSCOPY             FACILITY ROOM B64 (ASHWORTH 3
BASEMENT) - AUTHORISED USERS FOR WORK INVOLVING
CONTAINMENT LEVEL 3 SAMPLES
The Undersigned acknowledge the rules of the CoP Confocal Microscopy Facility,
Ashworth 3 and have demonstrated competence for SOP III of the CoP to the
signed authorized user

Date     User name/Email               Authorized by: Name
         Signature                                   Signature
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3                                                                                 19
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LABORATORY DECOMMISSIONING CHECKLIST - CONFOCAL MICROSCOPY FACILITY ROOM B64 (ASHWORTH 3)                                                   APPENDIX 7

Actions required:                                                                                             COMPLETED BY              CHECKED BY
                                                                                                                Name    Date            Name    Date
Check all biological materials have been removed from the lab – check benches, equipment, sink,
floors etc.
Check all lab coats have been removed.

Check all wastes have been taken away – do not sign off until everything has been removed.

Check warning labels on door have been removed.

Clean all sinks that may have been contaminated with biological materials – this includes any that have
been used whilst the lab is at CL3.
Clean all benches and surfaces that may have been contaminated with biological materials – this
includes any that have been used whilst the lab is at CL3.
Clean, and disinfect as appropriate, any items of equipment that may have been contaminated with
biological materials – this includes anything that has been used whilst the lab is at CL3.
Clean, and disinfect as appropriate, any other areas that may have been contaminated with biological
materials - this includes anything that has been used whilst the lab is at CL3 such as the floor.

Declaration:
This laboratory has been left in a condition such that it is safe for any personnel to enter without taking any precautions against exposure to
biologicalaterials.


Print name: ……………………………….….………….. Signature: …………..……………………………..……..………. Date:……………….




School: ……………………………………………………….…... Unit/Institute: ……………..………………………………………………………



                     LEAVE THIS CHECKLIST WITH THE FACILITY MANAGER. TAKE A COPY FOR YOUR RECORDS IN THE HOME CL3 LAB
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3                                              20
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LIST OF SUPPORTIVE DOCUMENTS FOR USE OF CONFOCAL MICROSCOPY FACILITY


DOCUMENT      TITLE                                            URL (TO BE COMPLETED)
1             ON-OFF-CLEANING (QUICK GUIDE)
2             LAS-SOFTWARE (QUICK GUIDE )
3             SP5 SAFETY
4             SP5 FULL MANUAL




              LEAVE THIS CHECKLIST WITH THE FACILITY MANAGER. TAKE A COPY FOR YOUR RECORDS IN THE HOME CL3 LAB
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3                                                                              21
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CHARGES FOR FACILITY USE: (STATUS 24.04.2008)                                                                             APPENDIX 9

General comment:
Please contact facility manager to discuss how to start your project and find out how charges apply.

CHARGES TO BE COSTED INTO GRANT APPLICATIONS:

The instrument has been funded by A REI Grant from the BBSRC (grant ref BB/E012442/1) and for this reason two different costing schemes apply:

FUNDING SOURCE                                                              Rate/h (as of 24.04.2008)
BBSRC Grants                                                                £ 31.94
Grants of other agencies                                                    £ 56.33




                     LEAVE THIS CHECKLIST WITH THE FACILITY MANAGER. TAKE A COPY FOR YOUR RECORDS IN THE HOME CL3 LAB

								
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