B.D.Singh Biotechnology by ozz15916

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									                           Syllabus Biotechnology
                               B.Sc. V Sem.
Paper – I: - Recombinant DNA, Plant and Animal Biotechnology
UNIT: -I
    (1) Recombinant DNA technology
    (2) Restriction endonucleases – types, nomenclature and
             recognition sequences
     (3)     Vectors
UNIT: -II
     (1)     Chemical synthesis of genes
     (2)     Gene Libraries
     (3)     Gene Amplification - PCR - apparatus, types, principle and
             application
UNIT: -III
     (1)     Animal tissue culture
     (2)     Hybridoma technology and Monoclonal antibodies
     (3)     In vitro fertilization and embryo transfer in human
UNIT: -IV
     (1)     Transgenic animals
     (2)     Cell culture products
     (3)     DNA finger printing
UNIT: -V
     (1)     Plant tissue culture- general techniques, nutrient media,
             callus
     (2)    Cloning and regeneration
     (3)    Anther, meristem, ovary and embryo culture
References: -
            (1)   Molecular Biotechnology – Bernard R.Glick and Jack J. Pasternak
            (2)   Plant cell, tissue and organ culture – O.N. Gamborg

            (3)   Gene Biotechnology - S.N. Jogdanad
            (4)   Biotechnology and Genomics - P.K. Gupta
            (5)   Plant Tissue Culture - K.G.Ramawat
            (6)   Biotechnology - B.D. Singh




                     Syllabus Biotechnology
                          B.Sc. V Sem.
Paper – II: - Immunology and Immunological techniques

UNIT: -I
    (1)     Introduction and History
    (2)     Normal flora of Human body
    (3)     Infection and its types
    (4)     Mechanism of pathogenesis

UNIT: -II

      (1)   Organs of immune system
      (2)   Cells of immune system-

             T-cells- its types and receptors

             B-cells- its receptors

      (3)   The complement system, mode of activation, classical and

             alternative pathway
UNIT: -III

      (1)    Immunity- Innate and acquired (active and passive)
      (2)    Immunity- Humoral and cell mediated
      (3)    Host defense mechanism- First, second and third line of host

              defense

UNIT-IV

     (1)     Antigens- properties, types and adjuvants
     (2)     Immunoglobulins- structure, types, properties and

             functions

     (3)     Antibodies- generation

UNIT: -V
     (1) Major incompatibility complex
     (2) Hypersensitivity
     (3) Auto immune diseases
References: -
             (1)   Immunology - Janis Kuby
             (2)   Essentials of Immunology - Ivann Roitt et.al
             (3)   Principles of Immunology - N.V. Shashtri
             (4)   Immunology - Saras publication




                             List of Practicals
                             B.Sc. V Semester


1. DNA Extraction from Plant tissue.
2. DNA Extraction from Animal tissue.
3. DNA Extraction from Bactrial cell.
4. RNA Extraction from Animal tissue.
5. RNA Extraction from Plant tissue.
6. Amplification of genetic material through PCR.
7. Quantitative estimation of DNA and RNA.
8. Separation of Protein by Column Gel Chromatography.
9. DNA agarose gel electrophoresis.
10.Protein electrophoresis(SDS-PAGE)




                       B.Sc. V Semester
                Scheme of Practical Examination
                        Biotechnology


Time: -3hrs                                          Max marks: -50


  1. Isolation of DNA/RNA from Plant/Animal tissue/Bacterial
     cell.                                                         10
  2. Separation of Protein by Column Gel Chromatography/
    Amplification of genetic material through PCR.                 10
  3. Quantitative estimation of DNA or RNA/ separation of DNA or
    Protein by gel electrophoresis.                                10
  4. Spotting                                                      10
  5. Viva-voce                                                     05
  6. Sessional                                            05
                                                      ….......
                                                          50




                         Syllabus Biotechnology
                              B.Sc. VI Sem.
Paper – I: - Modern Biotechnology
UNIT: -I
    (1)      Somatic hybridization
    (2)      Somaclonal variation
     (3)     Transgenic plants
UNIT: -II
     (1)     Immobilization of enzyme
     (2)     Uses of enzymes in solution
     (3)     Biofertilisers and biocontrol agents
UNIT: -III
     (1)     Biotransformation and bioleaching
     (2)     Biodegradation of xenobiotic compounds
     (3)     Single cell protein
UNIT: -IV
     (1)     Disease prevention (vaccines)
      (2)   Disease diagnosis
      (3)   Disease treatment
UNIT: -V
      (1)   Gene therapy
      (2)   Forensic medicine
      (3)   Northern hybridization and DNA microarray
References: -
            (1)   Molecular Biotechnology - Bernard R.Glick and Jack J. Pasternak
            (2)   Plant cell, tissue and organ culture – O.N. Gamborg
            (3)   Gene Biotechnology - S.N. Jogdanad
            (4)   Biotechnology and Genomics - P.K. Gupta
            (5)   Plant Tissue Culture - K.G.Ramawat
            (6)   Biotechnology - B.D. Singh




                           Syllabus Biotechnology
                                 B.Sc. VI Sem.
Paper – II: - Immunology and Biophysics
UNIT: -I
    (1) Immune response- primary and secondary
    (2) Antigen- antibody reaction, precipitation, agglutination,
         flocculation and immunofloresence

    (3) Latex and Haemagglutination, ELISA, RIA, ODD and RID
UNIT: -II
    (1) Immunotolerance
      (2) Transplantation immunology
      (3)    Immunodeficiency diseases
UNIT: -III
      (1)    Development of Cancer
      (2)    Causes of Cancer
      (3)    Tumor viruses
      (4)    Oncogenes and protoncogenes
UNIT: -IV
      (1) Bioenergetics
      (2) Isotopes, radioactivity and biological uses of radioactivity
      (3) Nuclear magnetic resonance
UNIT: -V
      (1) X-ray crystallography
      (2) Isoelectricfocusing and 2-dimensional gel electrophoresis
      (3) HPLC, Column and Partition Chromatography
References: -
            (1)   Immunology - Janis Kuby
            (2)   Essentials of Immunology - Ivann Roitt et.al.
            (4)   Principles of Immunology - N.V. Shashtri
            (5)   Immunology - Saras Publication
            (6)   Biophysics - Upadhyay and Nath
            (7)   Basic Biophysics For Biologist – M. Daniel
                          List of Practicals
                         B.Sc. VI Semester


1. Double Immunodiffusion (Ouchterlony Immunodiffusion)
2. Radial Immunodiffusion
3. Latex Agglutination
4. WIDAL Test
5. VDRL Test
6. Sandwich ELISA




                      B.Sc. VI Semester
               Scheme of Practical Examination
                         Biotechnology


Time: -3hrs                                       Max marks: -50
  1. Double Immunodiffusion (Ouchterlony Immunodiffusion)/   10
     Radial Immunodiffusion test.
  2. Sandwich ELISA/ Latex Agglutination test.               12
  3. WIDAL Test/ VDRL Test                                   08
4. Spotting      10
5. Viva-voce     05
6. Sessional     05
               ……..
                 50

								
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