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									 An IACUC and ARF Newsletter



Animal Care Matters
New Per Diem Rates                   Protocol Review
   The University’s Institutional    Change in Investigator
Animal Care and Use Committee
                                         All requests for change in the             SPRING 2002
(IACUC) has approved the ani-
                                     Principal Investigator (PI) of an
mal per diem rates for FY 2002-
                                     I A C U C -a p p r o v e d p r o t o c o l   I N S I D E TH I S I S SU E:
2003. These rates will become
                                     should include an original signed            From the IACUC Chair             2
effective July 1, 2002.
                                     Assurance Statement from the
    The IACUC has also directed                                                   The Lab Mouse                    2
                                     new PI. By doing so, the new PI
that Animal Resource Facilities      assures the IACUC that all work              New Per Diem Rates           Insert

(ARF) adjust per diem and other      in the protocol involving labora-            Rodent Surgery                   3
reimbursable rates annually to       tory animals will be conducted in            Quarantine & Stabilization       3
reflect changes in the costs for     accordance with the Guide for the
                                                                                  IACUC Members                    4
procuring and maintaining ani-       Care and Use of Laboratory Ani-
mals for teaching and research.      mals (the Guide), the Animal
Annual adjustments will allow        Welfare Act (PL 89-544 and
animal investigators to plan their   amendments), and the University
animal use over the life of a pro-   Policies.
ject. Currently, costs for animal
care such as feed, bedding, and
                                     NIH/AUP Review
caging have been increasing at an
annual rate of 3 to 5%. Investi-         For new Animal Use Proposal
gators submitting grant applica-     (AUP) submissions where NIH is                  2002 IACUC Meetings
tions should consider providing      the project’s funding source, the                                   AUP
                                                                                          Date         Deadline
for increases in animal costs when   PI is required to submit one copy
                                                                                        Apr 4             Mar 25
considering multiyear budgets. A     of the entire grant, including the                 May 2             Apr 22
table with the new rates can be      SPAR Proposal/Award Processing                     Jun 6
found in this newsletter as an in-   Form Page and the NIH Face                        May
                                                                                     27
sert and may also be viewed at the   Page (Form Page 1). A signature                    Jul 11            Jun 24
ARF website (http://                 on the second space in the Assur-                  Aug 1             Jul 22
uscm.med.sc.edu/ARF/                 ance Statement is also needed as                   Sep 5             Aug 26
                                                                                        Oct 3             Sep 23
index.htm).                          a certification that all animal pro-               Nov 7             Oct 28
    For questions on these new       cedures described in the grant ap-
rates, please contact Dr. Robert     plication are included and accu-
Beattie at 777-8106 or               rately described in the AUP.
PAGE 2                                                                                        ANIMAL CARE MATTERS



From the IACUC Chair
   If you still haven’t heard, or if you’ve                   There will be a couple of changes in the
heard it through other means, yes, it’s true,             review of protocols. First, if a request for a
the costs of keeping laboratory animals at                change in PI is to be made, an Assurance
USC will increase starting the first of July.             Statement that is signed by the new PI should
The IACUC made this recommendation last                   be submitted to the IACUC. Second, if NIH
January after careful review of the costs of              is the funding source for an animal proposal,
maintaining animals at ARF.      Please bear              a complete copy of the grant application
these new rates in mind when you figure the               should be submitted with the AUP.
budget allocation in your next grant applica-                 These changes are necessary. These are
tion.                                                     measures we have to take to further ensure
   The IACUC has also adopted guidelines                  that USC continues to achieve the highest
on the quarantine and stabilization of all                standards for laboratory animal care and use.
laboratory animals received and maintained                The IACUC expects everyone’s full coopera-
by ARF. We want to make sure that we use                  tion and support of these changes. Please call
healthy, stable animals that won’t compro-                me for any question or concern that you
mise our research results.                                may have.
                                                                                Dr. Donald O. Allen, Chair

The Common Laboratory Mouse
   No other mammal has been used in such                     Daily water . . . . . . . . . . . . . . 6 mls

numbers or in so many biomedical experi-                     GI transit time . . . . . . . . . . . 8–14 hrs
ments as the common laboratory mouse. But                    Heart rate . . . . . . . . . . . . . . . 330–780/min
how much do we really know about these                       Respiratory rate . . . . . . . . . . . 84–230/min
“most favored” specimen? The following bio-                  Blood volume . . . . . . . . . . . . 76–80 ml/kg
logical and physiological data about the lab                 Blood pressure: male . . . . . . 113–147 mm Hg
mouse, Mus musculus, may prove helpful es-                                       female . . . . 81-106 mm Hg
pecially to investigators and researchers when
                                                             Cycle length . . . . . . . . . . . . . 4–5 days*
designing their next experiments. Note that
                                                             Breeding life . . . . . . . . . . . . . 6–9 mos
the figures presented here are average.
                                                             Breeding age . . . . . . . . . . . . . 4–6 wks
   Adult body weight . . . . . . . . 30 gms
                                                             Litter size . . . . . . . . . . . . . . . . 5–10 pups
   Birth weight . . . . . . . . . . . . . 0.5–1.5 gms
                                                             Weaning age . . . . . . . . . . . . . 21 days
   Rectal temperature . . . . . . . . 99.3ºF
                                                             Hematocrit . . . . . . . . . . . . . . 39-49 %
   Diploid number . . . . . . . . . . 40
                                                             Hemoglobin . . . . . . . . . . . . . 10.2-16.6 mg/dl
   Life span . . . . . . . . . . . . . . . . 1–2 years
   Gestation period . . . . . . . . .     20 days
                                                         *postpartum estrus – receptive and fertile within hours
   Daily Feed . . . . . . . . . . . . . . 4–6 gms        after delivery
SPRING 2002                                                                             PAGE 3




Rodent Surgery
   General requirements for rodent surgery        undergo survival surgical procedures. Animal
are outlined in the Guide for Care and Use of     users have the responsibility to provide ro-
Laboratory Animals (the Guide). The Asso-         dents with adequate intra– and post-
ciation for Assessment and Accreditation of       operative care.
Laboratory Animal Care International                   All surgical procedures should be de-
(AAALAC) endorses the Guide standards for         scribed in the Annex C of the AUP and ap-
rodent surgery by requiring their accredited      proved by the IACUC. The protocol should
institutions to have adequate procedures in       also describe intra– and postoperative care,
place for conducting rodent surgery. Federal      aseptic techniques, use of anesthetics, analge-
Regulations and Policies regarding care and       sics, tranquilizers, and/or other treatments.
use of laboratory animals stress the impor-       A list of all personnel who will perform the
tance of minimizing pain and distress in all      surgical procedures, monitoring, and care
animal subjects, particularly those that will     should also be included.



Quarantine and Stabilization of Animals
   The IACUC has established the following        fore their use. Such a period allows the ani-
guidelines for the quarantine and stabiliza-      mal to recover from shipping stress, adapt to
tion of all animals received and maintained       its new surroundings, and become physio-
by the Animal Resource Facilities (ARF).          logically stable. Adequate acclimation times
                                                  may vary depending on the animal species,
    An effective quarantine minimizes the
                                                  source, type and duration of transportation,
chance for introduction of pathogens into an
                                                  and the intended use for the animals.
established colony. Information from the
source on animal quality should be sufficient     ⇒   For rodents, the recommended adapta-
to enable the Attending Veterinarian to de-           tion is 72 hours (3 days), minimum, prior
termine the length of quarantine, to define           to use in non-acute procedures.
the potential risks to personnel and animals      ⇒   For all large animals (including rabbit)
within the colony, and, to determine whether          undergoing non-acute procedures, the
therapy is required before animals are released       recommended adaptation is 5 days,
from quarantine. Rodents might not require            minimum.
quarantine if data from the source are suffi-         All newly arrived animals that shall be
ciently current and complete, and if the po-      used in teaching, research, or testing at USC
tential for exposure to pathogens during          should be provided the minimum acclima-
transit is considered.                            tion periods described in these guidelines.
   However, all newly received animals at         Failure to allow animals to acclimatize could
USC should be given a period for physiologic,     adversely affect animal health and research
psychologic, and nutritional stabilization be-    data.
                                                                         Campus Mail




ANIMAL CARE MATTERS
An IACUC and ARF Newsletter

   Animal Resource Facilities
      School of Medicine
Graduate Science Research Center
Columbia, South Carolina 29208

    Phone: 803-777-8106
     Fax: 803-777-2849
  E-mail: pooser@gwm.sc.edu




          We’re on the Web !!
        http://uscm.med.sc.edu/
             ARF/index.htm




A       nimal Care Matters is pub-
        lished four times a year by the
Institutional Animal Care and Use Com-       IACUC MEMBERSHIP
mittee (IACUC) and Animal Resource
Facilities (ARF) of the University of             CHAIRMAN
South Carolina (USC).                           Donald Allen, Ph.D.
      The IACUC is an institutional body             MEMBERS
appointed by the USC President to over-           Nathan Alderson
see the program for the humane care and        Robert Beattie, D.V.M.
use of all vertebrate animals used for          James Carson, Ph.D.
research, teaching, and training. Any           Wayne Carver, Ph.D.
investigator who intends to use labora-         Destiny Hollis, Ph.D.
tory animals must submit an Animal Use       Alexander McDonald, Ph.D.
Proposal (AUP) to the IACUC for its             Marjorie Peña, Ph.D.
review and approval.                              Margaret Rentz
      The ARF provides care and mainte-        Kenneth Walsh, Ph.D.
nance of all animals used by investiga-        Marlene Wilson, Ph.D.
tors. Preventive care is provided through       Bao Ting Zhu, Ph.D.
vendor animal health evaluations, quar-
antine programs, and sentinel animal          COMMUNITY MEMBERS
diagnostics. Special care and services can        Janice Ayers
be provided upon request.                        Joe Hick, M.D.
      Comments and submissions for               CONSULTANTS
Animal Care Matters are welcome and              Tommy Coggins
should be directed to Benilda P. Pooser,         Charles Jeffcoat
Ph.D., IACUC Administrator, at 777-
8106 or pooser@gwm.sc.edu.
                                 Animal Per Diem Rates 2002-2003
                                           Effective July 1, 2002


ANIMAL TYPE/
SPECIES                 PER DIEM    DISPOSAL                                 NOTES
Rodents
Mouse                                           Per diem is per cage. Whenever possible, mice are housed as
                                                compatible groups of up to 5 adult mice per standard cage.
      Conventional      .39        .05          Includes microisolator top
      Special care      1.22       .05          Sterile cage, feed, water (SCID, Nude)
Peromyscus              .35        .05          Per cage
Rat
      Conventional      .56        .26
      Special care      .84        .26          Sterile cage, feed, water, frequent change, etc.
      Female & litter   .56        .79
Gerbil                  .39        .05
Guinea pig              .60        .45
Hamster                 .39        .05


Rabbit                  1.46       2.43


Other Mammals
Pig                     6.89       24.25
Sheep/Goat              6.07       24.25        Indoors housing
Cat                     2.43       2.43
Dog                     4.08       24.25


Non-mammals
Frog (Rana sp.)         .56        .05
Turtle                  .56        1.22
Xenopus                 7.88       .05          Room per diem


Miscellaneous
Pig transport           115.76                  Transport from Clemson farm unit to ARF
Pig conditioning        42.50

								
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