AP/IB Biology Name: Review Worksheet for 12 AP Labs Period: Use the following website to review the 12 AP Biology labs. There is a link for this assignment on my website. WEBSITE: http://www.phschool.com/science/biology_place/labbench/ 1. Write the name for each of the 12 AP Biology labs below. Lab #1: Lab #7: Lab #2: Lab #8: Lab #3: Lab #9: Lab #4: Lab #10: Lab #5: Lab #11: Lab #6: Lab #12: 2. Highlight the labs that were not done in class using the list you wrote above. II *For each lab write the title in the spaces provided next to each lab number. LAB #1: A. From the website open the link for the first lab and answer the following questions: B. How is water potential calculated? + C. Name each symbol in the formula ( ) = –iCRT represents: –i: C: R: T: D. Take the Quiz and write the CORRECT answers below: 1. 2. 3. 4. 5. LAB #2: A. Name the two factors that effect enzyme action. B. Looking at the “Design of the experiment”, what is being investigated? C. Name the enzyme being investigated. D. Why is KMNO4 needed? What will it be used for? E. How is the rate of the reaction calculated? F. Sample Problem: (See the Analysis & Results). The correct answer is: *Quiz: Write the CORRECT answers for each question. 1. 2. 3. 4. 5. LAB #3: A. Give three differences between Mitosis & Meiosis B. Go to “Analysis and Results”. In the table below, write the name of each phase and determine how many cells are in each phase. Phase Number of Cells *Quiz: Write the CORRECT answers for each question. 1. 2. 3. 4. 5. LAB #4: Write 3 Questions after visiting this Lab Activity. 1. 2. 3. *Lab Quiz I: Write the CORRECT answers for each question. 1. 2. 3. 4. 5. *Lab Quiz II: Write the CORRECT answers for each question. 1. 2. 3. 4. 5. LAB #5: A. What are three ways we can measure cellular respiration? B. Why is KOH needed in order for the respirometer to work? C. When assembling a respirometer give the order of items that you need to place inside the vial. D. How are the three respirometers different? #1: #2: #3: E. What temperature should the water bath be? F. Calculate the rate of oxygen consumed from the “Analysis & Results I” and write the correct answer here. *Quiz: Write the CORRECT answers for each question. 1. 2. 3. 4. 5. LAB #6: A. What are the two parts (activities) to this lab? Part I: Part II: B. Name the bacteria used in the first part of this lab: C. The small circular pieces of DNA found in this bacteria is called a(n): D. DNA molecules that carry genes for specialized functions are called: E. What gene are we interested in moving from the bacteria for this lab? F. Name the four phases that bacteria go through while they’re alive. G. Name the chemical used to help make the bacterial cells “competent” enabling foreign DNA to be incorporated. H. What temperature is required in order to “Heat-shock” the bacteria? (Look at the design of the experiment) I. Which step of the procedure does the plasmid, carrying the ampicillin resistant gene (ampR), enter into the bacterial cell. (examine the “closer look” steps) J. Do Exercise 2 and place the five figures in the correct order. Write the correct order below. 1. 2. 3. 4. 5. K. Go to the Analysis & Results Section and answer the following questions: -How many agar plates should be prepared? -How many of these plates will ampicillin added? -Click on “next ” and write the correct letter for each plate: Plate I: Plate II: Plate III: Plate IV: *Quiz: Take the quiz and write the correct answer for each question 1. 2. 3. 4. 5. 6. LAB #6: Part II A. What does “Palindromic” mean? (See concept I) B. At what temperature are restriction enzymes incubated? C. In what direction will DNA move toward when subjected to an electrical current? D. DNA fragments that are (smaller, larger) will move farther in a gel than fragments that are (smaller, larger) in size. E. How many samples will you use in this lab? F. What organism will the DNA come from? G. Name the two restriction enzymes that you will mix the DNA samples with. H. Once you have made your gel, how much of the DNA sample will you load into the wells? I. When you run your gel and attach the electrical current to it, which lead will you attach to the well side of your gel (positive or negative)? J. How are we able to “track” the DNA after the gel is run? K. Go to: “Analysis & Results II”. After reading the information click on the next page and determine the approximate distances that each band migrated using the ruler provided. Write your answers in the table below. Actual Base Pairs (bps) Distance migrated (mm) 23130 9416 6557 4361 2322 2257 564 L. Why does a standard curve for Hind III need to be done in this lab? (See the “Making a standard curve” page) M. Do “Practice Problem 1” and write your answers in the table below. Distance migrated Interpolated Fragment (mm) Size (bps) Fragment 1 Fragment 2 Fragment 3 N. Do “Practice Problem 2” Determine the length of the two fragments for DNA I and write your answers in the table below. Distance migrated Interpolated Fragment (mm) Size (bps) Fragment 1 Fragment 2 *Quiz: Take the quiz and write the correct answers for the eight questions asked. 1. 5. 2. 6. 3. 7. 4. 8. Lab #7: A. Name the organism that will be analyzed for this lab. Write the genus and species name. B. What two patterns of inheritance will be observed with these organisms? C. How is a sex-linked trait different from an autosomal trait? Sex-linked: Autosomal: D. Name the structure on the leg of this organism that can be used to distinguish between male & female. E. Name two other features that can be used to determine sex. F. Look at the five flies five flies shown and write next to each number if they are male or female. 1. 2. 3. 4. 5. G. Look at the “Overview” for this experiment. How long does this experiment take? H. What can you do to anaesthetize the flies so that you can inspect them? , , or I. Name the four common types of mutations that can occur? (See the “Closer Look” pg) 1. 2. 3. 4. J. Do Exercise 1: Why is it important to remove the adults when the eggs and larvae appear? Do Exercise 2: After setting up a new vial with some of the flies from the F2 generation, what generation will their offspring be? K. Analysis & Results: Read the information on “reviewing the procedure” go to the “next” page, and answer the questions. Case 1: Was the cross monohybrid or Dihybrid? Was the cross sexlinked or autosomal? Case 2: Answer to question 1. Answer ro question 2. L. Chi Square Anaylsis of Data: What does this test determine? *Quiz: Take the quiz and write the correct answers for the five questions asked. 1. 2. 3. 4. 5. LAB #8: A. Name the mathematical model hat will be used in this lab. B. If a population is at “genetic equilibrium” and is stable five conditions have to be met list them below. 1. 4. 2. 5. 3. C. A random, nonselective change in the allele frequency in a population is called: D. Looking at each concept for these five conditions, name the type of organism used to explain each concept be looking at each link. Concept 1: Concept 4: Concept 2: Concept 5: Concept 3: E. Looking at “Concept 7”, Answer the following questions. -What does the Hardy Weinberg equation help determine? -What does “p” and “q” represent? p= q= -Why are p and q equal to 1? F. Looking at “Concept 8”. Write the CORRECT answers to the 4 questions. 1. 2. 3. 4. G. Concept 9: Sample Problem 2. The correct answer is: H. Concept 10: Sample Problem 3: The correct answer is: I. Looking at the “Design of the Experiment” what will be the starting allele frequency? . *Quiz: Take the quiz and write the correct answers for the five questions asked. 1. 2. 3. 4. 5. LAB #9: A. After reading the introduction what are the two sections of this lab that you will investigate? Section 1: Section 2: B. What other AP Bio lab should you review before doing this lab? C. Take a closer look at how water moves up the xylem in a plant. Name the two forces pointed out in the animation that helps to move water up the xylem. D. Concept 1: How many hydrogen bonds can each water molecule form? E. Concept 2: Where are most of the stomata located on a leaf? Name the two cells pointed out in the animated figure. F. Test Yourself: Answer the three questions and write the correct answers on the blanks provided. 1. 2. 3. G. Concept 3: Name the cells that surround each stoma: Name the ions that help to cause turgidity. Does the water potential increase or decrease as ions accummulate in the cells. Does water go in out of the cells? H. Concept 4: Write the four steps needed for stomata to close. 1. 2. 3. 4. I. Concept 5: Write the basic equation for photosynthesis in the space below. Equation: Plants must balance two things what are they? J. Concept 6: Write the CORRECT answer for the four questions asked. 1. 2. 3. 4. LAB #9: Continued K. When you set up “design” your experiment a special devise is needed to measure the rate at which a plant draws up water. Name this device. List the four elements needed to make this device. L. After looking at the overview for EXPT #1: List the four steps you need to take. 1. 2. 3. 4. M. When determining the surface area of a leaf what do you need? What do you do with the squares that are half filled? Do you need to include the petiole? Write the answer to the question posed: The approximate surface area is: Analysis & Results: Fill in the CORRECT answers in the table. Condition Total water loss Leaf Surface Leaf surface Water loss in after 30 min area cm3 area in m2 ml/m2/30 min Room (control) 0.004 ml 10 Bright light 0.006 ml 12 Fan (wind) 0.008 ml 10 Mist 0.002 ml 12 Lab Quiz 1: Answer the eight questions posed and write the CORRECT answers below. 1. 2. 3. 4. 5. 6. 7. 8. Key Concepts II: List the four tissues labeled on the diagram. 1. 2. 3. 4. Lab Quiz 2: Take the quiz and write the correct answers for the five questions asked. 1. 2. 3. 4. LAB #10: A. Name the two parts of this lab: Part 1: Part 2: B. The two organisms used in the lab will be: and Part 1: C. Key Concepts I: Where is blood pressure the highest? D. Concept 1: Name the two key components of blood pressure. and Which component occurs when the ventricles are relaxed? Is the pressure higher or lower? LAB #10: Part I (continued) E. Concept 2: what does the value “110” represent? F. Concept 3: Measuring blood pressure requires an instrument called : G. Concept 4: How much pressure should the cuff of this instrument be inflated to? H. Concept 5: How long do you feel your pulse to determine you heart rate? I. Design of Experiment: How many different tests of cardiovascular fitness will you conduct in this lab? J. Analysis of Results: Write the CORRECT answers to Questions #1-5. 1. 2. 3. 4. 5. *Lab Quiz 1: Write the CORRECT answers to question #1-4. 1. 2. 3. 4. LAB #10 Part II: Write five questions after reading through lab. 1. 2. 3. 4. 5. *Lab Quiz 2: Write the CORRECT answers to question #1-4. 1. 2. 3. 4. LAB #11: A. Name the two basic types of behaviors that are exhibited by animals. and B. Name two ways that scientists study behavior: and Concept 1: What type of behavior will be examined in Drosophila? Write the six behaviors shown in the sketch. Concept 2: Name the organism used for this concept. How many questions are posed that you should watch for? Concept 3: What do they teach you to do for this concept? Name the organism they used for their example. Concept 4: What are the organisms placed in? How is each side of this item different? C. Analysis and Results: What kind of respiratory structure is present in this organism? Where will most of these organisms be after 10 minutes into the experiment? Is this considered a controlled experiment? Explain why or why not? How is a control group different from an experimental group? D. Sample Size: When assembling a proper sample size what do you need to remember? How many pill bugs were used in the sample depicted in the figure? *Lab Quiz: Write the CORRECT answers to question #1-4. 1. 2. 3. 4. LAB #12: A. The author of this lab is: B. How many concepts will be explored in this lab? C. Concept 1: Oxygen is essential for: Name the five factors that can effect the amount of dissolved oxygen in water. 1. 4. 2. 5. 3. D. Concept 2: Write the CORRECT answers for questions #1-3. 1. 2. 3. E. Concept 3: Name the two key aspects of primary productivity. 1. 2. Why can’t we measure gross productivity directly? When can Respiration be measured in plants? Net Productivity = - F. Concept 4: Primary Productivity can measured in 3 ways name them. G. Design of Experiment: Read the nomograph and write the correct answer for the last two questions. % % LAB #12: (continued) H. The model used for productivity as a function of depth requires you to use BOD bottles. How many of these bottles will you need for this experiment? What can you wrap the bottles with to decrease light entering? Take a close look at each model. How may can you observe? I. Analysis and results: Fill in the blanks below. - = Respiration - = Gross Productivity - = Net Productivity Fill in the table below: % Light Bottle Gross Productivity Net Productivity 0 2 10 25 65 100 Skip the graphing exercise and go to the Lab quiz. *Lab Quiz: Write the CORRECT answers to question #1-4. 1. 2. 3. 4.
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