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List of Items for Molecular Work All items quoted by langstonwalker

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									                                   Annexure I
List of Items for Molecular Work

All items quoted should be of Molecular Biology Grade
S. No.   Item                                                           Quantity
1        QIAamp DNA Mini kit for 50 samples (Cat No. 51304)             2
2        QIAamp DNA Blood Midi kit for 100 samples Cat No. 51185)       7
3        pBR 322 DNA/Bsu (Hae III) Marker 0.05 mg                       3
4        phi x 174 DNA/Bsu RI (HaeIII) Marker 0.05 mg                   4
5        100 bp DNA Ladder (Fermentas) 0.05 mg                          2
6        100 bp DNA Ladder Plus (Fermentas) 0.05 mg                     1
7        High Range DNA Ladder (Fermentas) (Cat. No. SM1351) 0.05 mg    1
8        Hotstar Taq DNA polymerase (Qiagen)                            1000 U
9        Expand Long Template PCR System (Roche) 150 U                  1
10       Taq DNA Polymerase (3 Unit/? l) Bangalore Genei                5000 units
11.      Taq DNA Polymerase (Fermentas) (1U/? l)                        3000 units
12.      Taq DNA Polymerase (Bioron)                                    3000 units
13.      Set of 4 dNTPs (100 mM of each)                                2
14.      7-Deaza-2’                     -triphosphate (Roche) 200 ? l
                      -deoxy-guanosine-5’                               1
15.      Primers 10 nmoles, Salt Free (1416 bps)
16.      Restriction Endonucleases
         a) Mbo II (NEB Cat. No. # R0148S)                              300 U
         b) Xba (NEB / Fermentas)                                       1500 U
         c) Hind III (NEB / Fermentas)                                  10000 U
         d) Ava II (NEB / Fermentas)                                    800 U
         e) Mnl I (NEB / Fermentas)                                     1500 U
         f) Xmn 1 (NEB / Fermentas)                                     1000 U
         g) Hha 1 (NEB / Fermentas)                                     2000 U
         h) Taq 1 (NEB / Fermentas)                                     3000 U
         i) Hinf 1 (NEB / Fermentas)                                    4000 U
         j) Bcn 1 (NEB / Fermentas)                                     1000 U
         k) Acy 1 (Hin 1I) (NEB / Fermentas)                            300 U



Last date to receive quotation is 27.5.09

								
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