NIH SF424 Major Equipment Template

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					                                      NIH SF424 Major Equipment Template:

Notes: Please delete all instructions and examples before uploading this form. List the most
important equipment items already available for this project, noting the location and pertinent capabilities of

All examples, located in each section below, are kindly provided by Dr. Ralph Isberg from a grant funded several years
ago. Please delete the examples before beginning, and this blue text before PDF conversion and uploading.

                                               MAJOR EQUIPMENT

Some of the Major Equipment within laboratory space: 3 major workstation microscopes. Workstation 1:
Zeiss axioskop fluorescence/visible compound microscope with Nomarski optics fitted for photomicroscopy
and Hamamatsu Orca 100 controlled by Macintosh G4 containing IpLab Spectrum and deconvolution
software; Workstation 2: Nikon inverted phase microscope with Nomarski and Hoffman optics, electronic and
computer driven Sutter fluorescence filter wheel and shutter system, electronic and computer driven
Uniblaster shutter on halogen lamp, heated stage, Dage CCD analog camera for real time analysis, Hamamatsu
Slow Orca2 digital camera for low light applications, and Eppendorf miroinjection setup, all driven by
Macintosh G4 computer equipped with IPLab spectrum software and appropriate drivers. Workstation 3:
Zeiss inverted phase microscope with automated X,Y,Z axis control, emission and excitation filter wheels fitted
for YFP-CFP FRET as well as standard microscopy, with Hamamtsu OrcaER camera and OpenLab controller
software. Nikon inverted phase microscope for routine tissue culture work; 2 laminar flow hoods (rated for
Class 2 microorganisms); 2 CO2 incubators; 3 static air incubators; 3 stationary and 2 rotary water baths;
colony counter; 2 -80oC freezers within laboratory; Mettler balances; Beckman uv/vis spectrophotometer; Flow
microtiter spectrofluorometer fitted for 96 well plates; Kodak Image Station densitometer allowing
quantitation of luminescence detection of blots; Kodak gel documentation system; Brauda circulating
refrigerated. heated water bath; a number of Eppendorf centrifuges, gel boxes, Western Transfer apparatuses,
power supplies for sequencing and day-do-day operations; sonicator; pH meter; refrigerated table top
centrifuge fitted for microtiter plates; BioRad microtiter spectrophotometer for ELISA and cell binding assays;
two Gilson fraction collectors and peristaltic pumps; Beckman high speed utility centrifuge; three liq. N2
storage tanks. In addition we own a complete Pharmacia FPLC apparatus in a double-door chromatography

In common rooms: French pressure cells and automated laboratory press; Beckman ultracentrifuges with
range of rotors for use by Departmental members; 2 HPLCs; 2 Beckman liquid scintillation counters. In
addition we have access to EM facility having TEM and scanning microscopy. We are major users of this
facility, and a trained EM technician who sections our samples for a fee. We also have free access to an
Anatomy Dept. cryostat sectioner, which have used frequently and will use in this proposal. The Anatomy
Dept. has a digital confocal microscope driven by a Silicon Graphics workstation that is run by a full time
technician, and we have had unlimited access to this facility. We have unlimited access to Applied Biosystems
protein microsequenator, oligonucleotide synthesizer, mass spectrometer (capable of MS-MS applications) and
peptide synthesizer in a facility run by Mr. Michael Byrne from the Dept. of Physiology. This service is
provided on a pay for use basis. A fulltime technician runs the FACS and sorting facility on a fee for service
basis. The FACS is used by members of laboratory without technical help, whereas the sorter is run by the