PROPAGATION, EX SITU CONSERVATION AND CHARACTERIZATION OF MENTHA CERVINA AND MENTHA PULEGIUM Delgado-Sousa,F.1*; Rosa, C.; Sousa,T.; Amaro–Silva,,C.1; Oliveira,Mª R.1 1Escola Superior Agrária de Castelo Branco; Quinta Sra de Mércules; Apartado 119 6001-909 Castelo Branco *firstname.lastname@example.org Species Characterization and Regional Interess The ecotypes in study of Mentha genera, from the Labiteae family, are either spontaneous or cultivated in the Portuguese region of Beira Interior, Objectives by their importance as aromatic, culinary and medicinal herbs. Study the best propagation method VIAN A DO C ASTEL O BR AGA VILA R EAL BR AGAN Ç A POR TO Botanical Commom Proprieties Regional Chemical Plant of Mentha cervina and Mentha AVEI RO VISE U GUA R DA name names uses components parts pulegium LEIR IA COI MB RA PEN AM AC OR CA STELO BR AN C O ID AN HA -A -N OV A Cosmetic Poejo do peixe Drinks "Miga de peixe"(*) E. oil with high % of Flavours menthol Leaves Inflorescens SAN TAR ÉM POR TALE GR E Mentha cervina Unknwon Flavour in cigars, Sauces Stem Morphological characterization to LISB OA ÉVOR A Legenda: Poleol de cérvol cigarettes and Roots pipe tobacco In study on Agro nº 800 Con ce lh os IDA NHA -A -NO VA PE NA MA CO R identify correctelly the accessements SETÚ BAL BEJA Po rt uga l Escala: 1/2 3000 0 0 N Medicinal Tea to bronquite E. oil with 90% polegon Leaves Poejo Oil used in flavours and asthma Menthol Inflorescens FA RO Mentha pulegium Pennyroyal Detergents "Miga de peixe"(*) Flavonoids Stem Poleol d'aigua Household products Licours Carotenoids Roots Repellent to insects, Sauces Tannins specially fleas Abortificant activity Antiseptic (*) Soup made with fish river and bread Material and Methods Germination tests Vegetative Propagation Assays Morphological Characterization 100 seeds of Mentha pulegium (3 months Terminal cuttings of Mentha cervina and old), in 4 layers of wash filter paper Mentha pulegium Colour chart (RHS-2001) Conditons: 16h/25ºC day and 8h/20ºC nigth Growth regulator IBA 0,5% Morphological descriptors elaborated Turf and perlite (2:1) in Program AGRO Nº 34 Germination percentage is based on the The experimental design was completely emergency of the radicules randomized:two species; two treatments (T, IBA); four replications Results Vegetative Propagation Germinatio Morphological Characterization n Ecotypes Mentha cervina 1 2 100 Root % Stem 75 shapes round square 50 25 hairs calyx calyx and stem 0 simple leaves ensiform ovale-lanceolate IBA T margins entire serrulate 40 days 100 100 points of attachement sessile sub-sessile 15 days 100 100 n bracts ovate-lanceolate Treatment Inflorescence verticillaster forming a long spike verticillaster forming a long spike terminal head terminal head Mentha pulegium Ecotype: Mentha pulegium Flower hermaphrodite hermaphrodite 100 Seed size(mm): 0,6 calyx campanulate tubular 75 Root % simple dentate Seed colour: Orange grey 4 teeth (triangulars) 5 teeth (2 narrow+3 triangulars) 50 25 corolla pink white (RHS-N155D) violet (RHS-(85A) Number of seeds per g: 978 campanulate campanulate 0 IBA T stamens dehiscence extrorse extrorse Germination %: 66 Blooming months VII - X VI - X 40days 100 100 21days 93 100 Cientific Name, according Mentha cervina (L) Mentha pulegium (L) Tre atme nt Franco (1984) Figure 1- Root percentage and precocity, according to the vegetative propagation method used Conclusions Phenological evolution The results show that the most efficient mean of propagation was the 150 Num ber of days vegetative one. This further suggests that, in the future, this method of 130 110 propagation will be of the utmost importance in the production, 90 Floration phase Vegetation phase 70 preservation and conservation of the above mentioned species. 50 Mentha Mentha According to other works in Portugal, we didn’t find seeds in Mentha cervina pulegium Figure 2- Phenological evolution, from March to July 2002 cervina, whose results are only in vegetative propagation. A characterization/demonstration field was established with the plants References Franco, J. A. 1984. Nova Flora de Portugal (Continente e Açores). Volume II. Clethraceae- propagated in autumn/winter 2002. The plants were morphologically Compositae. Sociedade Astória, Lda. Lisboa.pp: 172-185 Mickey, M. & King, C.2002. The Cambridge Illustrated Glossary of Botanical Terms.Cambridge characterized so as to identify and to correctly classify botanically, each University Press.United Kingdom specie (considering that they have different origins in the Beira Interior Rosa, C.2003.Plantas Aromáticas e Medicinais da Beira Interior-Propagação, Conservação e Caracterização.Relatório de Fim de Curso de Engª de Ciências Agrárias-Ramo Agrícola. ESA- region). IPCB.Castelo Branco Sousa, T.2003.Ensaios de Técnicas Experimentais de Germinação de Sementes para a Conservação In this project we are working with other species of AMP, until ex-situ de Plantas Aromáticas e Medicinais. Relatório de Fim de Curso de Engª de Ciências Agrárias-Ramo Agrícola. ESA-IPCB.Castelo Branco 2004.The staff pretends to implement in-situ and ex-situ conservation Acknowledgments inserting plants in original places of native flora and establish a tematic This experiment was carried out on behaf of Program Agro nº 34 “Etnobotany, the use and management of aromatical and medicinal plants and their sustainable utilization as a contribution to Botanic Garden at ESACB, to contribute to sustainable development, the improvement of rural areas”, in “Escola Superior Agrária” (School of Agriculture of the Polytechinc Institute), Castelo Branco – Portugal. environmental education and awareness of AMP diversity.