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					DNA Fingerprinting

DNA Fingerprint

Step One

• Collect the sample! • What do you think are the most common sources of DNA samples for forensic scientists?

Step Two

• Extract and purify the DNA. • Much the same as what we did with the Strawberry!!

Step Three
• Cut the DNA! • What do we use the cut DNA molecules?

Step Four
• Sort the DNA by length! • Big pieces = slow • Small pieces = fast

Sort by Length
• DNA has a negative charge. • Electrophoresis machine has a positively charged probe on one end.

Step # 5
• DNA is split and transferred to a nylon sheet.

• A nylon sheet is blotted into the gel and DNA fragments are transferred onto it.

Step # 6

• Radioactive probes are attached to the DNA fragments.

Lets practice!!!
• DNA strand: AATCCGAATC
• DNA strand: TACCGATTCG

Step # 7
* Nylon

sheet with DNA fragments attached to radioactive markers is put into an x-ray machine.

End Result!!!

Step 8

• Analyze and compare results!

Do we have a match?
blood stain Bob Sue Juan Lakisha

Do we have a match?
blood stain Bob Sue Juan Lakisha


				
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posted:5/14/2008
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Lynne Conner Lynne Conner
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