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Material Safety Data Sheet
`acc. to ISO/DIS 11014
Date Prepared: 10/15/2008 Reviewed On: 12/21/2007

1 Identification of substance:
· Product name: Acridine Orange Stain · Catalog number: 212536 · Manufacturer/Supplier: BD Diagnostic Systems 7 Loveton Circle Sparks, MD 21152 Telephone: (410) 771 - 0100 or (800) 638 - 8663 · Information department: Technical Services · Emergency information: In case of a chemical emergency, spill, fire, exposure, or accident contact BD Diagnostic Systems (410) 771-0100 or (800)-638-8663, or ChemTrec at (800) 424-9300.

2 Composition/Data on components:
· Chemical characterization · Description: Mixture consisting of the following components. · Dangerous components: 7647-01-0 hydrochloric acid

3.6%

3 Hazards identification
· Hazard description: Xi Irritant · Information pertaining to particular dangers for man and environment R 36/37/38 Irritating to eyes, respiratory system and skin. · Classification system The classification was made according to the latest editions of international substances lists, and expanded upon from company and literature data. · NFPA ratings (scale 0-4)

1

0

0
1 0

Health = 1 Fire = 0 Reactivity = 0

· HMIS ratings (scale 0-4)
HEALTH FIRE

REACTIVITY 0

Health = 1 Fire = 0 Reactivity = 0

4 First aid measures
· General information No special measures required. · After inhalation Seek medical treatment in case of complaints. · After skin contact Immediately wash with water and soap and rinse thoroughly. · After eye contact Rinse opened eye for 15 minutes under running water. Then consult a doctor. · After swallowing Immediately call a doctor.
(Contd. on page 2)
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Material Safety Data Sheet
`acc. to ISO/DIS 11014
Date Prepared: 10/15/2008 Product name: Acridine Orange Stain
(Contd. of page 1)

Reviewed On: 12/21/2007

· Information for doctor Show this product label or this MSDS.

5 Fire fighting measures
· Suitable extinguishing agents CO2, ABC multipurpose dry chemical or water spray. Fight larger fires with water spray or alcohol resistant foam. · Special hazards caused by the material, its products of combustion or resulting gases: During heating or in case of fire toxic gases may be produced. · Protective equipment: No special measures required.

6 Accidental release measures
· Person-related safety precautions: Not required. · Measures for environmental protection: Wipe up with damp sponge or mop. · Measures for cleaning/collecting: No special measures required. · Additional information: No dangerous substances are released.

7 Handling and storage
· Handling · Information for safe handling: Ensure good ventilation/exhaustion at the workplace. · Information about protection against explosions and fires: No special measures required. · Storage · Requirements to be met by storerooms and receptacles: Store only in the original receptacle. · Information about storage in one common storage facility: Do not store together with alkaline material. Store away from oxidizing agents. · Further information about storage conditions: Store in cool, dry conditions in well sealed containers.

8 Exposure controls and personal protection
· Additional information about design of technical systems: No further data; see item 7. · Components with limit values that require monitoring at the workplace: 7647-01-0 hydrochloric acid PEL (USA) Short-term value: C 7 mg/m³, C 5 ppm REL (USA) Short-term value: C 7 mg/m³, C 5 ppm TLV (USA) Short-term value: C 7.5 mg/m³, C 5 ppm NIC-C 2; C 2.98; NIC-A4 · Additional information: The lists that were valid during the creation were used as basis. · Personal Protective Equipment · General protective and hygienic measures The usual precautionary measures for handling chemicals should be followed. · Breathing equipment: Not required.
(Contd. on page 3)
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Material Safety Data Sheet
`acc. to ISO/DIS 11014
Date Prepared: 10/15/2008 Product name: Acridine Orange Stain
(Contd. of page 2)

Reviewed On: 12/21/2007

· Protection of hands: Chemical resistant gloves (i.e. nitrile, or equivalent). · Eye protection: Safety glasses · Body protection: Protective work clothing (lab coat).

9 Physical and chemical properties:
· General Information Form: Color: Odor: Liquid Light orange Characteristic

· Change in condition Melting point/Melting range: Not determined Boiling point/Boiling range: Not determined · Flash point: · Auto igniting: · Danger of explosion: · Vapor pressure at 25°C: · Density at 25°C: Not applicable Product is not self igniting. Product does not present an explosion hazard. 23.0 hPa 0.999 g/cm3

· Solubility in / Miscibility with Soluble Water: · pH-value: Slightly acidic

10 Stability and reactivity
· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications. · Dangerous reactions Reacts with alkali and metals · Dangerous products of decomposition: No dangerous decomposition products known.

11 Toxicological information
· Acute toxicity: · Primary irritant effect: · on the skin: Irritant to skin and mucous membranes. · on the eye: Irritating effect. · Sensitization: No sensitizing effects known. · Additional toxicological information: The product shows the following dangers according to internally approved calculation methods for preparations: Irritant
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(Contd. on page 4)

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Material Safety Data Sheet
`acc. to ISO/DIS 11014
Date Prepared: 10/15/2008 Product name: Acridine Orange Stain
(Contd. of page 3)

Reviewed On: 12/21/2007

12 Ecological information:
· Ecotoxical effects: · Other information: The ecological effects have not been thoroughly investigated, but currently none have been identified. · General notes: Water hazard class 1 (Self-assessment): slightly hazardous for water.

13 Disposal considerations
· Product: · Recommendation Must not be disposed of with solid waste. Must adhere to official regulations. · Uncleaned packagings: · Recommendation: Disposal must be made according to state and federal regulations. · Recommended cleansing agent: Water, if necessary with cleansing agents.

14 Transport information
· DOT regulations: · Hazard class: · TDG class: · TDG (Transport dangerous goods): · Maritime transport IMDG: · IMDG Class: · Marine pollutant: No · Air transport ICAO-TI and IATA-DGR: · ICAO/IATA Class: -

15 Regulations
· SARA Section 355 (extremely hazardous substances) 7647-01-0 hydrochloric acid · SARA Section 313 (specific toxic chemical listings) 7647-01-0 hydrochloric acid · TSCA (Toxic Substances Control Act) 7732-18-5 water 127-09-3 sodium acetate 7647-01-0 hydrochloric acid 532-32-1 sodium benzoate
(Contd. on page 5)
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Material Safety Data Sheet
`acc. to ISO/DIS 11014
Date Prepared: 10/15/2008 Product name: Acridine Orange Stain
(Contd. of page 4)

Reviewed On: 12/21/2007

· Carcinogenicity categories · IARC (International Agency for Research on Cancer) 7647-01-0 hydrochloric acid · NTP (National Toxicology Program) None of the ingredients is listed. · TLV (Threshold Limit Value established by ACGIH) 7647-01-0 hydrochloric acid · Canadian Substance Listing · Canadian Domestic Substance List (DSL) 7732-18-5 water 127-09-3 sodium acetate 7647-01-0 hydrochloric acid 532-32-1 sodium benzoate · Canadian Ingredient Disclosure List (limit 0.1%) None of the ingredients is listed. · Canadian Ingredient Disclosure List (limit 1%) 7647-01-0 hydrochloric acid

3

A4

· Product related hazard information: Observe the general safety regulations when handling chemicals This product has been classified according to the hazard criteria of the Controlled Products Regulations (CPR), and the MSDS contains all of the information required by the CPR. · Hazard symbols: Xi Irritant · Hazard-determining components of labeling: hydrochloric acid · Risk phrases: 36/37/38 Irritating to eyes, respiratory system and skin. · Safety phrases: 1/2 Keep locked up and out of the reach of children. 26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. 45 In case of accident or if you feel unwell, seek medical advice immediately.

16 Other information:
To the best of our knowledge, the information contained herein is accurate. However, neither Becton, Dickinson and Company or any of its subsidiaries assumes any liabilities whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we can not guarantee that these are the only hazards that exist. · Department issuing MSDS: Safety and Environment Department MSDS created by Michael J. Spinazzola · Contact: Technical Service Representative
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