Skills Critical to Use of Aquaculture Drugs Treatment concentration calculations and filling out forms Presented at: Coldwater Fish Culture Course Bozeman, Montana March 18, 2008 Aquaculture Aquatic Animal Drug Approval Partnership: In Pursuit of Aquaculture Drug Approvals Module overview Calculations • Static bath treatments 100% active ingredient <100% active ingredient (powder and liquid) • Flow-through treatments 100% active ingredient • Medicated feed treatments Amount of feed to order for 10 d treatment Amount of pre-mix to add to feed to achieve the target concentration INAD forms VFD forms T&E forms Calculations There are a variety of ways to calculate amount of drug needed to treat fish at a certain concentration Use resources such as Piper et al. 1982 or Wedemeyer 2001 Always have somebody double-check your calculations If you’ve never used a water-borne treatment, conduct a small-scale bioassay on a few fish Calculations for Prolonged Bath Treatments Volume of water to be Target Correction treated x concentration x factor (gal) (ppm or mg/L) Strength of chemical Amount of chemical = needed (g) Calculation for Constant-Flow Treatments Flow Treatment Target Correction rate x time x concentration x factor Amount of (gpm) (min) (ppm or mg/L) chemical = needed Strength of chemical (g) Static bath 100% active ingredient (e.g., chloramine-T, AQUI-S®) • Need to know Tank or raceway dimensions (L x W x D) and capacity (ft3) or Volume of water (gal) Desired treatment concentration (ppm or mg/L) Correction factor (constant) for g/gal (0.00378) For example – static bath chloramine-T – dry powder Determine how much chloramine-T is needed to treat fish in a raceway that is 16’ long x 4’ wide x 1.5’ deep to achieve a target dose of 15 mg/L 1st - 16’ x 4’ x 1.5’ = 96 ft3 2nd - 96 ft3 x 7.48 gal/ft3 = 718.1 gal 3rd - 718.1 gal x 15 ppm x 0.00378 = 40.7 g CLT For example – static bath AQUI-S® – liquid (weight) Determine how much AQUI-S® is needed to sedate fish in a 5-gal bucket filled with 3 gal water to achieve a target dose of 40 mg/L • 3 gal x 40 mg/L x 0.00378 = 0.45 g AQUI-S® Static Bath <100% active ingredient (e.g., 35% PEROX-AID®) • Need to know Tank or raceway dimensions (L x W x D) and capacity ( ft3) or Volume of water (gal) Desired treatment concentration (ppm or mg/L) Active ingredient (35% PEROX-AID® – in decimal format) Correction factor (constant) for g/gal (0.00378) If measuring volume (mL) instead of weight (g) - need specific gravity Volume of Target water to be x concentration Correction treated x factor (ppm or mg/L) (gal) Amount of ÷ S.G. = chemical Strength of chemical needed (mL) For example – static bath Anaganafanafon – dry powder (50% active) Determine weight anaganafanafon needed to treat fish in a raceway that is 60’ long x 6’ wide x 2.5’ deep to achieve a target dose of 50 mg/L 1st - 60’ x 6’ x 2.5’ = 900 ft3 2nd - 900 ft3 x 7.48 gal/ft3 = 6,732 gal 3rd - (6,732 gal x 50 mg/L x 0.00378) ÷ 0.50 = 2,545 g anaganafanafon For example – static bath 35% PEROX-AID® – liquid, 35% active specific gravity = 1.1317 Determine volume of 35% PEROX-AID® needed to treat fish in a tank that is 4’ in diameter x 1’ deep to achieve a target dose of 50 mg/L 1st – π (pi) x R2 x depth = 3.14 x 22 x 1 = 12.6 ft3 2nd - 12.6 ft3 x 7.48 gal/ft3 = 94.2 gal 3rd - (94.2 gal x 50 mg/L x 0.00378) ÷ 0.35 = 50.9 g 35% PEROX-AID® 4th - 50.9 g 35% PEROX-AID® ÷ 1.1317 = 45.0 mL 35% PEROX-AID® Flow-Through 100% active ingredient (e.g., formalin) • Need to know Flow rate (gpm) Treatment time (min) Desired treatment concentration (ppm or mg/L) Correction factor for g/gal (0.00378) For example – flow-through formalin 100% active Specific gravity = 1 Determine how much formalin is needed to treat fish in a raceway for 60 min (water flow, 100 gpm) to achieve a target dose of 167 ppm • 100 gpm x 60 min x 167 ppm x 0.00378 = 3,788 mL formalin Feed amounts To determine how much medicated feed is needed to complete a full treatment • Need to know Number of fish to be treated and estimated average fish weight Estimated total fish weight (g or kg) Feed rate (% bw) Treatment duration (days) For example – feed amounts Treatment duration = 10 d Feed rate = 1% bw 100,000 fingerling coho salmon Estimated average fish wt, 1.5 g Estimated total fish weight • 100,000 x 1.5 g = 150,000 g = 150 kg Amount of feed to be fed daily • 150 kg x 0.01 (1% bw) = 1.5 kg feed Amount of feed to be fed for 10 d • 1.5 kg feed/d x 10 d = 15 kg medicated feed Amount of Pre-mix to add to feed Need to know • Target concentration in feed (mg/kg) • Percent active ingredient in drug • Number of fish to be treated • Estimated average fish weight (g) • Feed rate (% bw) • Total amount of feed For example – Pre-Mix Aquaflor® (50% active florfenicol) Determine how much feed and Aquaflor® pre- mix is needed to treat at a concentration of 10 mg florfenicol/kg fish for 10 d when fed at a rate of 1% bw • Information provided 100,000 fingerling coho salmon Estimated average fish wt, 1.5 g Convert 10 mg flor/kg fish to 0.01 g flor/1,000g fish For example – Pre-Mix Aquaflor® (50% active florfenicol) Amount of feed needed: • Total weight = 150,000 g fish • Feed amount = 150,000 g fish x 0.01 = 1,500 g feed/d • Feed for 10 d = 1,500 g feed x 10 d = 15,000 g feed Amount of florfenicol needed • Amount of florfenicol (ffc) for 1 d = 150,000 g fish x 0.01 g ffc/1000 g fish = 1.5 g ffc • Amount of florfenicol for 10 d = 1.5 g ffc x 10 d = 15 g ffc • Amount of Aquaflor® needed 15 g ffc x (1.0 g Aquaflor/0.5 g ffc) = 30 g Aquaflor® For example – Pre-Mix Aquaflor® (50% active florfenicol) Amount of Aquaflor® to medicate 20 kg bag of feed • Calculate % of Aquaflor® premix in feed 30 g Aquaflor®/15,000 g feed = 0.002 Convert to % by multiplying by 100 = 0.2% • Determine amount of Aquaflor® to add to 20 kg bag of feed 20,000 g feed x 0.002 (0.2%) = 40 g Aquaflor® Forms INAD Forms • Sign-Up forms • Work sheet for study design • Report of receipt of drug • Drug inventory form • Results report form • Discharge worksheet Sign-Up Form Investigator and Monitor can not be the same person Worksheet for Study Design For example – chloramine-T Evaluate the effectiveness of….. Will you adhere to the withdrawal period? Have everybody to read the MSDS? Report of receipt of Drug Drug inventory form Results report form Treatment worked, no problems, no adverse effects Discharge worksheet When paperwork needs to be sent to AADAP Office Generic Examples of Completed Forms ... Form W - Worksheet for Designing Individual Field Trials Form 1 - Report on Receipt of Drug - Guide for Reporting Investigational New Animal Drug Shipments for Poikilothermic Food Animals Form 2 - Drug Inventory Form Form 3 - Clinical Field Trials: Results Report Form Addendum 2 - Discharge Worksheet (version 1) Addendum 2 - Discharge Worksheet (version 2) Form W - Worksheet for Designing Individual Field Trials Forms VFD Forms • VFD Form - Aquaflor T&E Forms • Drug receipt form • Drug inventory form VFD INFORMATION VFD Drugs: Impact on Feed Mills Serving the Aquaculture Industry (PDF) AquaFAQs 2: VFD Drugs and You: Understanding Your Role (PDF and HTML) VFD Form – AQUAFLOR® (PDF) VFD Form – AQUAFLOR®-CA1 (PDF) Establishing feed distributorship eases VFD process for fish farmers (PDF and HTML) http://www.aquaflor-usa.com/secondary/product_info/vfd.htm VFD Form http://www.aquaflor-usa.com/secondary/product_info/vfd.htm T&E Forms http://www.fws.gov/fisheries/aadap/SIGNUP.htm Finally, Any Questions??
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