Treatment concentration calculations and filling out forms by qvg16642

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									               Skills Critical to Use of
                 Aquaculture Drugs


    Treatment concentration
calculations and filling out forms

                              Presented at:
                       Coldwater Fish Culture Course
                            Bozeman, Montana
                              March 18, 2008


                                                          Aquaculture
  Aquatic Animal Drug Approval Partnership: In Pursuit of Aquaculture Drug Approvals
               Module overview
Calculations
• Static bath treatments
     100% active ingredient
     <100% active ingredient (powder and liquid)
• Flow-through treatments
     100% active ingredient
• Medicated feed treatments
     Amount of feed to order for 10 d treatment
     Amount of pre-mix to add to feed to achieve the target
     concentration

INAD forms
VFD forms
T&E forms
              Calculations

There are a variety of ways to calculate
amount of drug needed to treat fish at a
certain concentration
Use resources such as Piper et al. 1982 or
Wedemeyer 2001
Always have somebody double-check your
calculations
If you’ve never used a water-borne treatment,
conduct a small-scale bioassay on a few fish
Calculations for Prolonged Bath
          Treatments

Volume of
water to be            Target            Correction
 treated      x     concentration    x     factor
   (gal)            (ppm or mg/L)


                  Strength of chemical


                      Amount of
                       chemical
              =
                        needed
                          (g)
                Calculation for
           Constant-Flow Treatments


 Flow     Treatment           Target         Correction
 rate   x    time      x   concentration x     factor         Amount of
(gpm)       (min)          (ppm or mg/L)                      chemical
                                                          =    needed
               Strength of chemical                              (g)
                 Static bath

100% active ingredient (e.g., chloramine-T,
AQUI-S®)
• Need to know
    Tank or raceway dimensions (L x W x D) and
    capacity (ft3) or
    Volume of water (gal)
    Desired treatment concentration (ppm or mg/L)
    Correction factor (constant) for g/gal (0.00378)
     For example – static bath
         chloramine-T – dry powder

Determine how much chloramine-T is needed
to treat fish in a raceway that is 16’ long x 4’
wide x 1.5’ deep to achieve a target dose of 15
mg/L

1st - 16’ x 4’ x 1.5’ = 96 ft3
2nd - 96 ft3 x 7.48 gal/ft3 = 718.1 gal
3rd - 718.1 gal x 15 ppm x 0.00378 = 40.7 g CLT
     For example – static bath
         AQUI-S® – liquid (weight)

Determine how much AQUI-S® is needed to
sedate fish in a 5-gal bucket filled with 3 gal
water to achieve a target dose of 40 mg/L

• 3 gal x 40 mg/L x 0.00378 = 0.45 g AQUI-S®
                             Static Bath
 <100% active ingredient (e.g., 35% PEROX-AID®)
  • Need to know
         Tank or raceway dimensions (L x W x D) and capacity ( ft3) or
         Volume of water (gal)
         Desired treatment concentration (ppm or mg/L)
         Active ingredient (35% PEROX-AID® – in decimal format)
         Correction factor (constant) for g/gal (0.00378)
         If measuring volume (mL) instead of weight (g) - need specific
         gravity

Volume of               Target
water to be   x      concentration           Correction
 treated                                 x     factor
                     (ppm or mg/L)
   (gal)                                                               Amount of
                                                          ÷ S.G.   =   chemical
                  Strength of chemical                                  needed
                                                                         (mL)
    For example – static bath
Anaganafanafon – dry powder (50% active)

Determine weight anaganafanafon needed to
treat fish in a raceway that is 60’ long x 6’
wide x 2.5’ deep to achieve a target dose of
50 mg/L

1st - 60’ x 6’ x 2.5’ = 900 ft3
2nd - 900 ft3 x 7.48 gal/ft3 = 6,732 gal
3rd - (6,732 gal x 50 mg/L x 0.00378) ÷ 0.50 = 2,545 g
  anaganafanafon
    For example – static bath
 35% PEROX-AID® – liquid, 35% active
      specific gravity = 1.1317

Determine volume of 35% PEROX-AID®
needed to treat fish in a tank that is 4’ in
diameter x 1’ deep to achieve a target dose of
50 mg/L
1st – π (pi) x R2 x depth = 3.14 x 22 x 1 = 12.6 ft3
2nd - 12.6 ft3 x 7.48 gal/ft3 = 94.2 gal
3rd - (94.2 gal x 50 mg/L x 0.00378) ÷ 0.35 = 50.9 g
  35% PEROX-AID®
4th - 50.9 g 35% PEROX-AID® ÷ 1.1317 = 45.0 mL 35%
  PEROX-AID®
              Flow-Through

100% active ingredient (e.g., formalin)
• Need to know
    Flow rate (gpm)
    Treatment time (min)
    Desired treatment concentration (ppm or mg/L)
    Correction factor for g/gal (0.00378)
        For example – flow-through
                formalin 100% active
                 Specific gravity = 1

Determine how much formalin is needed to treat fish in a
raceway for 60 min (water flow, 100 gpm) to achieve a
target dose of 167 ppm

• 100 gpm x 60 min x 167 ppm x 0.00378 = 3,788 mL formalin
               Feed amounts

To determine how much medicated feed is
needed to complete a full treatment
• Need to know
    Number of fish to be treated and estimated average fish
    weight
    Estimated total fish weight (g or kg)
    Feed rate (% bw)
    Treatment duration (days)
   For example – feed amounts
        Treatment duration = 10 d
           Feed rate = 1% bw

100,000 fingerling coho salmon
Estimated average fish wt, 1.5 g
Estimated total fish weight
• 100,000 x 1.5 g = 150,000 g = 150 kg
Amount of feed to be fed daily
• 150 kg x 0.01 (1% bw) = 1.5 kg feed
Amount of feed to be fed for 10 d
• 1.5 kg feed/d x 10 d = 15 kg medicated feed
             Amount of Pre-mix
              to add to feed
Need to know
•   Target concentration in feed (mg/kg)
•   Percent active ingredient in drug
•   Number of fish to be treated
•   Estimated average fish weight (g)
•   Feed rate (% bw)
•   Total amount of feed
      For example – Pre-Mix
    Aquaflor® (50% active florfenicol)

Determine how much feed and Aquaflor® pre-
mix is needed to treat at a concentration of
10 mg florfenicol/kg fish for 10 d when fed at
a rate of 1% bw

• Information provided
    100,000 fingerling coho salmon
    Estimated average fish wt, 1.5 g
    Convert 10 mg flor/kg fish to 0.01 g flor/1,000g fish
        For example – Pre-Mix
    Aquaflor® (50% active florfenicol)
Amount of feed needed:
• Total weight = 150,000 g fish
• Feed amount = 150,000 g fish x 0.01 = 1,500 g
  feed/d
• Feed for 10 d = 1,500 g feed x 10 d = 15,000 g feed
Amount of florfenicol needed
• Amount of florfenicol (ffc) for 1 d
     = 150,000 g fish x 0.01 g ffc/1000 g fish = 1.5 g ffc
• Amount of florfenicol for 10 d
     = 1.5 g ffc x 10 d = 15 g ffc
• Amount of Aquaflor® needed
     15 g ffc x (1.0 g Aquaflor/0.5 g ffc) = 30 g Aquaflor®
      For example – Pre-Mix
    Aquaflor® (50% active florfenicol)

Amount of Aquaflor® to medicate 20 kg bag
of feed
• Calculate % of Aquaflor® premix in feed
    30 g Aquaflor®/15,000 g feed = 0.002
    Convert to % by multiplying by 100 = 0.2%
• Determine amount of Aquaflor® to add to 20 kg
  bag of feed
    20,000 g feed x 0.002 (0.2%) = 40 g Aquaflor®
                     Forms

INAD Forms
•   Sign-Up forms
•   Work sheet for study design
•   Report of receipt of drug
•   Drug inventory form
•   Results report form
•   Discharge worksheet
         Sign-Up Form




Investigator and Monitor can not be the same person
Worksheet for Study Design
For example – chloramine-T




                             Evaluate the effectiveness of…..




                             Will you adhere to the withdrawal period?




                             Have everybody to read the MSDS?
Report of receipt
of Drug
Drug inventory
form
Results report form


                      Treatment worked, no problems, no
                      adverse effects
Discharge
worksheet
When paperwork needs to be
sent to AADAP Office
Generic Examples of Completed Forms ...
Form W - Worksheet for Designing Individual
Field Trials

Form 1 - Report on Receipt of Drug - Guide
for Reporting Investigational New
Animal Drug Shipments for Poikilothermic
Food Animals

Form 2 - Drug Inventory Form

Form 3 - Clinical Field Trials: Results Report
Form

Addendum 2 - Discharge Worksheet (version
1)

Addendum 2 - Discharge Worksheet (version
2)
Form W - Worksheet for Designing Individual Field Trials
                   Forms


VFD Forms
• VFD Form - Aquaflor
T&E Forms
• Drug receipt form
• Drug inventory form
VFD INFORMATION
VFD Drugs: Impact on Feed Mills
Serving the Aquaculture Industry (PDF)
AquaFAQs 2: VFD Drugs and You: Understanding Your Role
(PDF and HTML)

VFD Form – AQUAFLOR® (PDF)
VFD Form – AQUAFLOR®-CA1 (PDF)
Establishing feed distributorship eases VFD process for
fish farmers (PDF and HTML)




     http://www.aquaflor-usa.com/secondary/product_info/vfd.htm
VFD Form




           http://www.aquaflor-usa.com/secondary/product_info/vfd.htm
              T&E Forms
http://www.fws.gov/fisheries/aadap/SIGNUP.htm
    Finally,


Any Questions??

								
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