# Treatment concentration calculations and filling out forms by qvg16642

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```									               Skills Critical to Use of
Aquaculture Drugs

Treatment concentration
calculations and filling out forms

Presented at:
Coldwater Fish Culture Course
Bozeman, Montana
March 18, 2008

Aquaculture
Aquatic Animal Drug Approval Partnership: In Pursuit of Aquaculture Drug Approvals
Module overview
Calculations
• Static bath treatments
100% active ingredient
<100% active ingredient (powder and liquid)
• Flow-through treatments
100% active ingredient
• Medicated feed treatments
Amount of feed to order for 10 d treatment
Amount of pre-mix to add to feed to achieve the target
concentration

VFD forms
T&E forms
Calculations

There are a variety of ways to calculate
amount of drug needed to treat fish at a
certain concentration
Use resources such as Piper et al. 1982 or
Wedemeyer 2001
Always have somebody double-check your
calculations
If you’ve never used a water-borne treatment,
conduct a small-scale bioassay on a few fish
Calculations for Prolonged Bath
Treatments

Volume of
water to be            Target            Correction
treated      x     concentration    x     factor
(gal)            (ppm or mg/L)

Strength of chemical

Amount of
chemical
=
needed
(g)
Calculation for
Constant-Flow Treatments

Flow     Treatment           Target         Correction
rate   x    time      x   concentration x     factor         Amount of
(gpm)       (min)          (ppm or mg/L)                      chemical
=    needed
Strength of chemical                              (g)
Static bath

100% active ingredient (e.g., chloramine-T,
AQUI-S®)
• Need to know
Tank or raceway dimensions (L x W x D) and
capacity (ft3) or
Volume of water (gal)
Desired treatment concentration (ppm or mg/L)
Correction factor (constant) for g/gal (0.00378)
For example – static bath
chloramine-T – dry powder

Determine how much chloramine-T is needed
to treat fish in a raceway that is 16’ long x 4’
wide x 1.5’ deep to achieve a target dose of 15
mg/L

1st - 16’ x 4’ x 1.5’ = 96 ft3
2nd - 96 ft3 x 7.48 gal/ft3 = 718.1 gal
3rd - 718.1 gal x 15 ppm x 0.00378 = 40.7 g CLT
For example – static bath
AQUI-S® – liquid (weight)

Determine how much AQUI-S® is needed to
sedate fish in a 5-gal bucket filled with 3 gal
water to achieve a target dose of 40 mg/L

• 3 gal x 40 mg/L x 0.00378 = 0.45 g AQUI-S®
Static Bath
<100% active ingredient (e.g., 35% PEROX-AID®)
• Need to know
Tank or raceway dimensions (L x W x D) and capacity ( ft3) or
Volume of water (gal)
Desired treatment concentration (ppm or mg/L)
Active ingredient (35% PEROX-AID® – in decimal format)
Correction factor (constant) for g/gal (0.00378)
If measuring volume (mL) instead of weight (g) - need specific
gravity

Volume of               Target
water to be   x      concentration           Correction
treated                                 x     factor
(ppm or mg/L)
(gal)                                                               Amount of
÷ S.G.   =   chemical
Strength of chemical                                  needed
(mL)
For example – static bath
Anaganafanafon – dry powder (50% active)

Determine weight anaganafanafon needed to
treat fish in a raceway that is 60’ long x 6’
wide x 2.5’ deep to achieve a target dose of
50 mg/L

1st - 60’ x 6’ x 2.5’ = 900 ft3
2nd - 900 ft3 x 7.48 gal/ft3 = 6,732 gal
3rd - (6,732 gal x 50 mg/L x 0.00378) ÷ 0.50 = 2,545 g
anaganafanafon
For example – static bath
35% PEROX-AID® – liquid, 35% active
specific gravity = 1.1317

Determine volume of 35% PEROX-AID®
needed to treat fish in a tank that is 4’ in
diameter x 1’ deep to achieve a target dose of
50 mg/L
1st – π (pi) x R2 x depth = 3.14 x 22 x 1 = 12.6 ft3
2nd - 12.6 ft3 x 7.48 gal/ft3 = 94.2 gal
3rd - (94.2 gal x 50 mg/L x 0.00378) ÷ 0.35 = 50.9 g
35% PEROX-AID®
4th - 50.9 g 35% PEROX-AID® ÷ 1.1317 = 45.0 mL 35%
PEROX-AID®
Flow-Through

100% active ingredient (e.g., formalin)
• Need to know
Flow rate (gpm)
Treatment time (min)
Desired treatment concentration (ppm or mg/L)
Correction factor for g/gal (0.00378)
For example – flow-through
formalin 100% active
Specific gravity = 1

Determine how much formalin is needed to treat fish in a
raceway for 60 min (water flow, 100 gpm) to achieve a
target dose of 167 ppm

• 100 gpm x 60 min x 167 ppm x 0.00378 = 3,788 mL formalin
Feed amounts

To determine how much medicated feed is
needed to complete a full treatment
• Need to know
Number of fish to be treated and estimated average fish
weight
Estimated total fish weight (g or kg)
Feed rate (% bw)
Treatment duration (days)
For example – feed amounts
Treatment duration = 10 d
Feed rate = 1% bw

100,000 fingerling coho salmon
Estimated average fish wt, 1.5 g
Estimated total fish weight
• 100,000 x 1.5 g = 150,000 g = 150 kg
Amount of feed to be fed daily
• 150 kg x 0.01 (1% bw) = 1.5 kg feed
Amount of feed to be fed for 10 d
• 1.5 kg feed/d x 10 d = 15 kg medicated feed
Amount of Pre-mix
to add to feed
Need to know
•   Target concentration in feed (mg/kg)
•   Percent active ingredient in drug
•   Number of fish to be treated
•   Estimated average fish weight (g)
•   Feed rate (% bw)
•   Total amount of feed
For example – Pre-Mix
Aquaflor® (50% active florfenicol)

Determine how much feed and Aquaflor® pre-
mix is needed to treat at a concentration of
10 mg florfenicol/kg fish for 10 d when fed at
a rate of 1% bw

• Information provided
100,000 fingerling coho salmon
Estimated average fish wt, 1.5 g
Convert 10 mg flor/kg fish to 0.01 g flor/1,000g fish
For example – Pre-Mix
Aquaflor® (50% active florfenicol)
Amount of feed needed:
• Total weight = 150,000 g fish
• Feed amount = 150,000 g fish x 0.01 = 1,500 g
feed/d
• Feed for 10 d = 1,500 g feed x 10 d = 15,000 g feed
Amount of florfenicol needed
• Amount of florfenicol (ffc) for 1 d
= 150,000 g fish x 0.01 g ffc/1000 g fish = 1.5 g ffc
• Amount of florfenicol for 10 d
= 1.5 g ffc x 10 d = 15 g ffc
• Amount of Aquaflor® needed
15 g ffc x (1.0 g Aquaflor/0.5 g ffc) = 30 g Aquaflor®
For example – Pre-Mix
Aquaflor® (50% active florfenicol)

Amount of Aquaflor® to medicate 20 kg bag
of feed
• Calculate % of Aquaflor® premix in feed
30 g Aquaflor®/15,000 g feed = 0.002
Convert to % by multiplying by 100 = 0.2%
• Determine amount of Aquaflor® to add to 20 kg
bag of feed
20,000 g feed x 0.002 (0.2%) = 40 g Aquaflor®
Forms

•   Sign-Up forms
•   Work sheet for study design
•   Report of receipt of drug
•   Drug inventory form
•   Results report form
•   Discharge worksheet
Sign-Up Form

Investigator and Monitor can not be the same person
Worksheet for Study Design
For example – chloramine-T

Evaluate the effectiveness of…..

Will you adhere to the withdrawal period?

Have everybody to read the MSDS?
Report of receipt
of Drug
Drug inventory
form
Results report form

Treatment worked, no problems, no
Discharge
worksheet
When paperwork needs to be
sent to AADAP Office
Generic Examples of Completed Forms ...
Form W - Worksheet for Designing Individual
Field Trials

Form 1 - Report on Receipt of Drug - Guide
for Reporting Investigational New
Animal Drug Shipments for Poikilothermic
Food Animals

Form 2 - Drug Inventory Form

Form 3 - Clinical Field Trials: Results Report
Form

Addendum 2 - Discharge Worksheet (version
1)

Addendum 2 - Discharge Worksheet (version
2)
Form W - Worksheet for Designing Individual Field Trials
Forms

VFD Forms
• VFD Form - Aquaflor
T&E Forms
• Drug receipt form
• Drug inventory form
VFD INFORMATION
VFD Drugs: Impact on Feed Mills
Serving the Aquaculture Industry (PDF)
AquaFAQs 2: VFD Drugs and You: Understanding Your Role
(PDF and HTML)

VFD Form – AQUAFLOR® (PDF)
VFD Form – AQUAFLOR®-CA1 (PDF)
Establishing feed distributorship eases VFD process for
fish farmers (PDF and HTML)

http://www.aquaflor-usa.com/secondary/product_info/vfd.htm
VFD Form

http://www.aquaflor-usa.com/secondary/product_info/vfd.htm
T&E Forms