The Microscope - Download as PowerPoint by fjwuxn

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									The Microscope
The Microscope

Spectroscopic Microscope
Light – Compound (Bright field)
Fluorescent
Confocal
Electron- Transmission and scanning
Fluorescence Microscopy




                          Confocal Microscopy
Illuminating system

Light source
Condenser lens- immediately below
 specimen
Iris diaphragm- controls amount of light
Imaging system

Objectives-4X, 10X, 40X
Ocular- lens to look through; monocular &
 binocular
Oculars magnify 10 times
Stage- place where specimen are mounted
Magnification

Magnification total= Mag obj X Mag ocu
Parfocal
Parcentered
Field of view
Stages of making a slide

Sectioning-blade
Mounting-(wet)- glycerin; water
Dehydration-alcohol
Fixing-formaldehyde; glutaraldehyde
Determining size of field

Micrometers
Ocular micrometer
Stage micrometer
FOV
Use of the micrometer

 Demonstration

								
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