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THE MEIJI COMPOUND MICROSCOPE

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					THE MEIJI COMPOUND MICROSCOPE
          EXERCISE 1
             COMPONENTS OF THE
              MEIJI MICROSCOPE
• Anton van Leeuwenhoek first used the
  microscope over 300 years ago.
• This allowed scientists to view cells for the
  first time.
• In this lab, we use the Meiji bright field
  compound microscope.
  – Bright field shows the field of view, or what you
    are looking at, light in color and the specimen,
    what you really want to see, darker.
MONOCULAR COMPOUND
    MICROSCOPE
BINOCULAR COMPOUND
    MICROSCOPE
              COMPONENTS OF THE
               MEIJI MICROSCOPE
  – This microscope is a compound microscope
    because it has two lenses that you are looking
    through, the first the eyepiece and the second
    located above the specimen.
    • Notice the ocular body, or eyepiece, is held on by a
      clamping screw and moves.
• Lens System
  – The ocular lens enlarges the object by 10 (10X).
    • Some have pointers in them and some do not.
  – The revolving nosepiece includes 4 objective
    lenses:
REVOLVING
NOSEPIECES
              COMPONENTS OF THE
               MEIJI MICROSCOPE
  •   4X scanning (red)
  •   10X low-power (yellow)
  •   40X high-power (blue)
  •   100X oil immersion (white)
– Microscopes increase image size by
  magnification.
  • Multiply the ocular lens by the objective lens to get the
    total magnification.
– Resolution reveals details of the specimen.
  • This measures the microscope’s ability to separate two
    points that are close together.
            COMPONENTS OF THE
             MEIJI MICROSCOPE
– Contrast is the difference between bright and
  dark on the image.
  • Contrast is controlled by adjusting the iris.
  • To increase you close it to reduce the light intensity
    entering the condenser, and the opposite to increase it.
– Parfocal and paracentric features on this
  microscope allow the changing of the objective
  lenses easily.
  • Parfocal allows the image to stay in focus when
    changing the lens.
  • Paracentric keeps the filed you are looking at stays in
    the center of the field.
              COMPONENTS OF THE
               MEIJI MICROSCOPE
  – As the lens is changed, the working distance gets
    smaller.
    • That is, the distance between the objective lens and the
      slide on the stage.
• Focus Knobs
  – There are two knobs, course and fine, which are
    located on both sides of the microscope.
    • Always use the course first with the scanning and low-
      power lens.
    • Use the fine with the high-power and oil immersion
      lenses.
MECHANICAL STAGE
             COMPONENTS OF THE
              MEIJI MICROSCOPE
• Mechanical Stage
  – This is the platform where the slide is placed and
    includes the slide holder.
  – The “X” and “Y” travel knobs move the slide in
    vertical and horizontal directions.
• Condenser System
  – This includes the on/off switch and the
    brightness control.
  – In the Meiji microscope, there is a two stage
    condenser, which is found the light source and
CONDENSER SYSTEM
           COMPONENTS OF THE
            MEIJI MICROSCOPE
  the mechanical stage.
– The illuminator condenser directs the light
  upward from the base to the substage
  condenser, which concentrates and refracts the
  light rays to direct it toward the specimen.
– The light needed to view the specimens varies
  according to the slice of the specimen and the
  stain used.
– The condenser should be just below the slide so
  that the maximum light will go through the
  specimen to the objective lens.
           COMPONENTS OF THE
            MEIJI MICROSCOPE
– There is a substage condenser focus knob that
  changes the vertical height of the substage
  condenser.
– The intensity of light is controlled by the iris
  diaphragm.
– The filter holder, beneath the iris diaphragm,
  should be closed so that light is not blocked from
  the illuminator condenser to the substage
  condenser.
– The field iris should always be open.
BUCCAL SMEARS
BUCCAL SMEARS

				
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posted:10/10/2010
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