Recipes:
10X PBS w/ azide 1X PBS w/ azide
Na2HPO4x7H2O 456g 10X PBS stock 2L
NaH2PO4xH2O 44g d.i. water 18L
NaCl 1635g (Fill to line on carboy.)
NaN3 20g
Dissolve in 3-4L hot d.i. water.
Dilute to 20L in carboy.
0.1M Borate Saline 1N HCl
Boric Acid 278.3g stock HCl 30ml
Sodium Borate 428.8g d.i. water 330ml
NaCl 197.3g *Add acid to water!
Dissolve in 3L hot d.i. water. (1:10 dilution=0.1N HCl)
Dilute to line on carboy-45L.
DEA Substrate Buffer (ELISA) 1mM HCl
milli-Q water 400ml stock HCl 0.042ml
MgCl2 24.5mg milli-Q water 499.958ml
DEA 48ml 2
QS to 500ml(52ml) and pH to 9.8
0.1M Tris HCl (0.5M NaCl) pH=8 0.1M Acetate buffer (0.5M NaCl) pH=4
Tris 6.057g Sodium acetate 6.804g
NaCl 14.61g NaCl 14.61g
milli-Q water 500ml milli-Q water 500ml
pH to 8 w/ stock HCl pH to 4 w/ stock Hcl
10X Electrode Running Buffer 40% Acrylamide/bis-Acrylamide
Tris 60g Acrylamide 40g
Glycine 288g bis-Acrylamide 1.07g
SDS 10g milli-Q water 100ml
milli-Q water 1L
1% Paraformaldehyde FACS Fixation Buffer
Heat 300ml d.i. water. Do not boil.
Add 2ml 1N NaOH. Remove from heat.
While stirring, add 5g of paraformaldehyde.
Let dissolve (5-10 min.) Add 2ml 1N HCl.
Add 50 ml 10X PBS w/ azide. Add 146ml d.i. water.
7.0
Add 5ml h.i. FCS.