Recipes: 10X PBS w/ azide Na2HPO4x7H2O 456g NaH2PO4xH2O 44g NaCl 1635g NaN3 20g Dissolve in 3-4L hot d.i. water. Dilute to 20L in carboy. 0.1M Borate Saline Boric Acid 278.3g Sodium Borate 428.8g NaCl 197.3g Dissolve in 3L hot d.i. water. Dilute to line on carboy-45L. DEA Substrate Buffer (ELISA) milli-Q water 400ml MgCl2 24.5mg DEA 48ml QS to 500ml(52ml) and pH to 9.8 1X PBS w/ azide 10X PBS stock 2L d.i. water 18L (Fill to line on carboy.)
1N HCl stock HCl 30ml d.i. water 330ml *Add acid to water! (1:10 dilution=0.1N HCl)
1mM HCl stock HCl 0.042ml milli-Q water 499.958ml 2