Toxoplasma gondii winona.edu

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Toxoplasma gondii By: Cory Polacek & Nicole Bertram Background  Obligate intracellular parasite  Found in many kinds of tissue including muscle and intestinal epithelium  One of most common human parasites in the world  First discovered in the gundi (N.African rodent) in 1908 Hosts Geographic Range  World wide  Less frequent in extreme temperatures and higher altitudes  Definitive hosts: members of cat family  Intermediate host: any warm blooded mammal or bird Life Cycle  Two Phases  Sexual  Asexual Life Cycle-Sexual  Cat is infected by ingesting oocysts or eating an infected intermediate  Bradyzoites or sporozoites penetrate cells of small intestine  Parasite undergoes asexual as well as sexual reproduction (sexual reproduction only occurs in cats  Oocysts are produced from sexual reproduction  Cat passes oocysts in fecal matter  Oocysts become infective after about 24 hours (sporulate)  Oocysts can them be ingested by an intermediate host… Life Cycle-Asexual  Intermediate host ingests bradyzoites or oocysts and infect macrophages in the mucosa of the small intestine  Within the macrophage tachyzoites develop  Tachyzoites rapidly asexually reproduce and are distributed throughout the host body  After several weeks parasite divides slower and produces zoitocysts filled with bradyzoites (inactive stage that forms after the immune response is triggered)  The infected intermediate can then be ingested by the definitive host or another intermediate. Tachyzoites (asexually reproducing) Oocyst (sexual reproducing) Sources of Infection  Accidental ingestion of infected cat fecal matter  Contaminated food/utensils from raw meat  Contaminated drinking water  Receiving infected organ transplant or blood transfusion (rare) Pathogenesis/Clinical Signs  Most cases are asymptomatic  Flu like symptoms, swollen lymph glands and muscle aches and pains  Some suffer from hepatitis, pneumonia, blindness and severe neurological disorders (acute infections)  Especially true for individuals with compromised immune systems (AIDS) Public Health Concerns  Risk to pregnant mothers and babies  If mother is infected prior to pregnancy there is virtually no risk of fetal transmission  If mother becomes infected during pregnancy fetus is at risk (congenital toxoplasmosis)  Fetal infection could result in still-birth or spontaneous abortion  70% of infants born with congenital toxoplasmosis are asymptomatic, but 8% show severe CNS impairment Diagnosis/Treatment  Diagnosis in humans is based on laboratory tests  Biopsy  ELISA  Pyrimethamine and sulfonamides are given together Control Methods  Freeze meat at -14° C if you like to eat it undercooked.  Reduce the number of stray cats.  Clean the litter box every day.  Keep sandboxes covered. Sources  http://www.emedicine.com/emerg/topic601.html  http://www.biosci.ohio-state.edu/~parasite/toxoplasma.html  http://www.cdc.gov/ncidod/dpd/parasites/toxoplasmosis/factsht_to xoplasmosis.htm  http://cvm.msu.edu/courses/mic569/docs/parasite/TOXO.HTML  http://en.wikipedia.org/wiki.Toxoplasma_gondi  Foundations of Parasitology Special Thanks  We would like to thank baby Gary for posing for our presentation  We would like to thank Gary, Kenya, Sushi and Mika for possibly infecting us with T.gondii  And thanks to all the cats in the world for possibly altering our personalities to become “sex kittens” !!!

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