general electrophoresis

Electrophoresis Timothy G. Standish, Ph. D. ©2000 Timothy G. Standish O HO P O CH2 O H N O O N O OH H P HO HO O O O H NH2 O D N A OH HO P O CH2 O O N N NH2 N H OH N O CH2 O O HO P O CH2 O O H O P OH OH O N N N NH H H2O NH2 O CH2 O P H OH H2O CH2 G T A A C G C - - - - - G C T A C G A T Major groove A T C G G C T A - - - - - Minor groove - - - - - - - The Watson - Crick Model Of DNA - T - - - - - ©2000 Timothy G. Standish Submarine Gel Electrophoresis Sample to be loaded Negative electrode Gel Box Positive electrode Well Gel ©2000 Timothy G. Standish Submarine Gel Electrophoresis ©2000 Timothy G. Standish Submarine Gel Electrophoresis 100 V DNA Migration + ©2000 Timothy G. Standish Gel Electrophoresis Wells ©2000 Timothy G. Standish Gel Electrophoresis Wells ©2000 Timothy G. Standish Gel Electrophoresis Wells Large Direction of DNA Travel Small + ©2000 Timothy G. Standish Electrophoresis of Dyes 1 2 3 4 5 ©2000 Timothy G. Standish ©2000 Timothy G. Standish