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Cardiovascular Microbiology

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Cardiovascular Infections Normal flora and defenses • Sterile site – Has NO normal flora. • Inaccessible ? (no – relatively easy and regular) • Unsuitable? (you have got to be kidding!) • PROTECTED? (Yes!) • Defenses – Mechanical (moving and “teflon” like endothelium) – Biological • Circulating immune cells and molecules • Reticulo-endothelial system Bacteria in bloodstream • Bacteraemia » Transient presence. » Organisms not multiplying. » Patient asympotomatic. » Important means of spread for other diseases. • Septicaemia » Organisms actively multiplying. » Acutely symptomatic Bacteraemia or Septicaemia Bacteria enter the bloodstream bacteraemia HOST - susceptibility Examples • Oral organisms and cardiac defects = endocarditis. •Meningococci and meningitis •Bowel organism and perforation. (Strongyloides bedtime septicaemia MICROBE -virulence -number Septicaemia • Symptoms – Fevers, chills, spiking temperature, rigors – Rash (petaechial) • Mortality – – – – Very high even with prompt therapy. Gram positive 10 – 20% Gram negatuve 25 – 40% Septic shock 40 – 90% Septic shock • Gram positive – some exotoxins. • Gram negative – all have endotoxin. Endotoxin Chemical mediators of HOST origin Endogenous pyrogen FEVER Vaso-actives SHOCK Clotting factors DIC MULTI ORGAN FAILURE You don’t “catch” septicaemia • Urinary tract infections – Pyelonephritis, catheterisation. • Respiratory tract – Pneumonia (pneumococci) – Colonization (meningococci) • Abscess – Intermittent seeding. • Surgical (and other) wounds – Site is an important modifier – INDWELLING MEDICAL DEVICES Venous catheter related BSI’s • Commonest cause of BSI’s in Australia. – 3500 cases annually (1.5 CR-BSI per 1000 admissions) – 25% case fatality (50% of which is directly attributable) • Location of venous line – Central line has 20X daily risk of peripheral line • Access – Down outside of catheter, in catheter, in infusate • Prevention / treatment – Minimize use and duration, optimize location. Collignon; Aus – Teams for insertion and care Prescriber – Antibiotics and REMOVAL Blood cultures Urine Blood Blood days Broth Blood cultures • Need a “specimen collection” and culture strategy to deal with small numbers of organisms that may be present intermittently. – Multiple collections (3 in 24 hours) – Maximum volume (do not overfill!) – Aseptic collection / handling technique is CRITICAL – What if patient has venous catheter? • Do not take from catheter line! Bacterial endocarditis • Infection of endocardium • Portal of entry - primary focus / event Prior Bacteraemic injury - minor breach Sticky phase endocardium transient Fibrin and platelets Bacteria adhere and multiply vegetation Valve dysfunction and emboli Bacterial endocarditis • ACUTE versus SUB ACUTE – Progression, virulence of organism, host factors • Diagnosis – Clinical (sounds), blood culture • Treatment – Prolonged aggressive combination therapy. • Prevention – Prophylactic antibiotics for those “AT RISK” • Previous endocardial damage or susceptibility • PLUS procedures producing bacteraemia
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