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					Gel electrophoresis
L Mathias

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Definition
• Separation of DNA fragments according to size, based on movement through a gel medium when an electric field is applied.

DNA negatively charged

DNA cut up using restriction endonucleases

Make up the gel which the DNA will be put into

Dye added to the DNA

Buffer solution added to the tank

DNA samples loaded into wells

Electrical current applied to the chamber

DNA is stained using ethidium bromide


				
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