So what is this stuff? Filamentous Algae Oedogonium Zygnema Free living algaes Algae Netrium (algae) Cladophora Protists Perenema Blepherisma Volvox / Paramecium Volvox Paramecium Euglena Stentor / Amoeba Amoeba Stentor C y c l o p s Cyclops (Copapod) Daphnia Daphnia Ostracod Cypris Ostracod Nematode Nematode (Round worm) Rotifers Rotifer Tardigrade Tardigrade (Water bear) Microscope parts and functions Making the Pond Water Slide • Place one or two drops with some algae on slide. • Carefully place coverslip at a 45 degree angle and touch the liquid. • Lower coverslip until it lies flat. Using the Microscope • Make sure the low power lens (4x) is clicked in place. (over the opening in the stage) • With the light on, look through the eyepiece adjust the diaphragm until you see a bright circle of light. • Place and fasten the prepared slide to the stage. Center some algae in the stage opening. • Lower the objective lens so it is at its closest point to the slide. (look from the side) Using the Microscope (cont.) • Looking through the eyepiece, slowly turn the coarse adjustment knob, moving the lens away until objects come into focus. • Move the slide over the stage with knobs or fingers until a specimen of interest is centered. • Observe specimens as desired. Then switch to medium or high power. Use the fine adjustment knob to adjust the focus. Pondwater Lab • On a piece of paper make drawings of 2 different organisms you see in a drop of pond water. Identify and label them from the photos. • Describe if you think each organism is single-celled or multi-celled. • Label any cell parts you can identify.