Generalized Relationship Between Anabolism & Catabolism by AmnaKhan

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									Pseudomonas spp.

Metabolic Pathways :
Generalized Relationship Between Anabolism & Catabolism

Metabolic Pathways :
More Specifically…

Metabolic Pathways : What is the goal of Anabolism-Catabolism? Biosynthesis of Cell Constituents like….

So how do Microbes Accomplish Anabolism-Catabolism ? There are a wide variety of redox reactions / metabolic pathways…

But first, What is “redox” to a microbe? But first, What is “redox” “Redox” is short for Reduction – Oxidation Reaction. Redox reactions are used by microbes to generate energy

The “Red-” in Redox is short for Reduction (A type of Catabolism) Metabolic Pathways : Reduction

The “-ox” in Redox is short for Oxidation (Another step in Catabolism) Metabolic Pathways : Oxidation

Metabolic Pathways : Biochemical Redox reactions are cyclic – two sides of the same coin

Metabolic Pathways : There are a variety of electron acceptors available ….

…but not all electron accepters (i.e. oxidants) are created equal

Metabolic Pathways : Energy from various redox reactions

Chemical vs. Biological Oxidation : Haste makes (metabolic) waste

Types of Redox Pathways in Microbes pathways in Microbes

Metabolic Pathways : Types of redox

Metabolic Pathways : Aerobic(Types of Catabolism) & Anaerobic


Metabolic Pathways : Aerobic Respiration

Microbial Metabolism; Aerobic Example

Metabolic Pathways : Anaerobic Respiration


Microbial Metabolism; Anaerobic Example

Microbial Interactions via Metabolic Pathways : the connection between anaerobic & aerobic metabolism

Eo (v) = +.82

Eo (v) = -.21

Eo (v) = -.24

Comparison of What Happens during Anaerobic & Aerobic Metabolism

WWTP Basic Layout

Oxidation Ponds : Aerobic

COD (Inorganic substrates)

BOD (Organic substrates

- Rely on algae to generate O2 for microbial aerobic metabolism

WWTP aerobic

COD (Inorganic substrates)

BOD (Organic substrates)

Oxidation Ponds : Anaerobic

BOD (Organic substrates) COD (Inorganic substrates)

Anaerobic sediment reactions

Facultative Oxidation Ponds

Oxidation Ponds : Facultative

WWTP facultative

WWTP metabolism

WWTP Basic Layout

The “tie in” back to the soil environment : Spray fields

Spray field diagram : low rate irrigation

Spray field diagram : overland flow

Spray field diagram : high rate infiltration

Septic systems

Soil Environment :

Soil Habitat : Metabolic Activity 1

Cation Bridging




Metabolic Pathways : Fermentation : is usually a precursor to further metabolic pathways (i.e. aerobic & anaerobic respiration)

The important difference between fermentations and respirations is that fermentations do not require and external electron acceptor Fermentation followed by respiration reduces the time/energy needed for a microbe to utilize a “tough” macromolecules

Metabolic Pathways : Fermentation :

Fermentation trade-offs : great if you don’t have a suitable electron accepter (i.e. O2, NO3, etc.) available BUT
This pathway generates the least energy per unit of initial organic compound

Microbial Inter-connections; Anaerobic digestion

Microbial Interconnections; Anaerobic digestion

Examples of bacterial fermentations 1

Natural Sources of Hexose

Syntrophy : Another type of symbiosis another bacterial fermentation by one species if microbe hooked to an oxidation by a second species


Review Metabolic Groups of Microbes

Photosynthesis the difference between Chemoautotrophy & Photoautotrophy

Photoautotrophic Catabolism

Catabolism are essentially the same among all prokaryotes, it involves some sort of respiration…

Photoautotrophic Anabolism

The main difference is in anabolism…

Metabolic Pathways : Photoautotrophy
Catabolism Anabolism

Microbial Interconnections; Example ; the link between photosynthetic microbes & metabolism (specifically) respiration by nonphotosynthetic microbes

Microbial Interactions via Metabolic Pathways : Relationship between Photosynthetic Prokaryotes & Heterotrophic Pro- and Eukaryotes

Microbial Inter-connections; Carbon Cycling : redox reactions / metabolic pathways

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