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					Transgenic technology
                  Transgenic technology
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   Directory
   Transgenic plants of several commonly used methods
   1. Agrobacterium-mediated transformation method
   2. Gene gun-mediated transformation method
   3. Pollen tube pathway
   Commonly used animal transgenic technology
   1. Microinjection
   2. Somatic cell nuclear transplantation
   Transgenic relationship between technology and traditional techniques
   1, transgenic plants
   2, transgenic animals
   Transgenic rice from the laboratory to field
   Origin of genetically modified food
   1, the safety of genetically modified food
   2, genetically modified food outlook
   3, transgenic crops, potential ecological risks
   4, harmful effects on the environment
   5 Related Reading
   GM commercial impact on the national economy and must be treated carefully for
commercial production of genetically modified staple food
   Transgenic plants of several commonly used methods
   1. Agrobacterium-mediated transformation method
   2. Gene gun-mediated transformation method
   3. Pollen tube pathway
   Commonly used animal transgenic technology
   1. Microinjection
   2. Somatic cell nuclear transplantation
   Transgenic relationship between technology and traditional techniques
   1, transgenic plants 2, transgenic animals, genetically modified rice from the
laboratory to the field of genetically modified foods origin of a transgenic food safety
issues 2, genetically modified food outlook 3, GM crops potential ecological risk 4,
harmful to the environment of 5 readings GM commercial impact on
people's livelihood
   Must be treated carefully for commercial production of genetically modified staple
food

GM
Use scientific means to extract from a biological need to genes transferred into
another organism so that the other genes with the reorganization, resulting in a
specific shape with good genetic material. Genetically engineered plants and animals
can change characters, breeding new varieties. Other organisms can also grow human
biological products needed for medicine, food and so on.
The definition of transgenic technology
And modified over the artificial separation of genes into the genome of organisms,
due to import gene expression traits of organisms can be caused by genetic
modification, this technology called transgenic technology (Transgene technology).
People often say that the "genetic engineering",
"genetic                engineering",              "genetic
transformation" are synonymous with GM. Modified by transgenic
organisms in the media is often referred to as "genetically modified
organisms off" (Genetically modified organism, referred to as GMO).
Transgenic technology, including the foreign gene cloning, expression vector, receptor
cells, and transgenic means such as, the foreign gene of artificial synthesis, gene
regulatory networks of artificial design development, leading to the 21st century,
transgenic technology will move toward synthetic biology times.
   Transgenic plants of several commonly used methods
Genetic transformation method according to the need by tissue culture, plant
regeneration can be divided into two categories, first regeneration through tissue
culture, commonly used methods are Agrobacterium-mediated transformation,
particle bombardment; another method does not require through tissue culture, mainly
the relatively mature pollen tube pathway.
   1. Agrobacterium-mediated transformation method
Agrobacterium tumefaciens is a ubiquitous Gram-negative bacteria in soil, under
natural conditions it can infect the majority of chemotaxis dicots injury site, and to
induce crown gall tumors or hairy roots. Agrobacterium tumefaciens and
Agrobacterium rhizogenes, respectively, in cells containing the Ti plasmid and Ri
plasmid, on which there is a T-DNA, Agrobacterium infection of plant through the
wound into the cells, T-DNA can be inserted into the plant genome. Therefore, the
Agrobacterium is a natural plant genetic transformation system. It will be the target
gene inserted into the T-DNA transformed area of infection with Agrobacterium
foreign genes to achieve the transfer and integration of plant cells, and then through
the cell and tissue culture, regeneration of the transgenic plants.
Agrobacterium initially only be used for dicotyledonous plants, in recent years, a
number of Agrobacterium-mediated transformation in monocots (especially rice) is
also widely used.
   2. Gene gun-mediated transformation method
Using gunpowder explosion or high pressure gas to accelerate (the acceleration device
is known as gene gun), will wrap up with a target gene of the DNA solution directly
into a complete high-speed micro-bomb plant tissues and cells, and then through the
cell and tissue culture , regenerate plants, selected positive transgenic plants which
shall be transgenic plants. Compared with the Agrobacterium-mediated
transformation, particle bombardment transformation of one of the main advantage is
not limited the scope of receptor plants. And its vector plasmid is relatively simple
and, therefore, the current application of transgenic research in a way more extensive.
   3. Pollen tube pathway
To the ovary after pollination combined target gene DNA injection solution, use of
plant in bloom, the formation of the fertilization process of pollen tube pathway, the
exogenous DNA into fertilized egg cells, and further integrated into the genome of
receptor cells with the development of a fertilized egg become a new individual with
GM. The method in the early 80s made by the Chinese scholar Zhou Guangyu,
China's largest current promotion is the use of transgenic cotton pollen
tube pathway nurtured. The big advantage of the method is not dependent on art

				
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