Borate saline buffer (0.1 M, pH 8.45) solubilized proteins from almond, Brazil nut, cashew nut, hazelnut, macadamia, pine nut, pistachio, Spanish peanut, Virginia peanut, and soybean seeds were prepared from the corresponding defatted flour.
inform July 2010, Vol. 21 (7) 455 Publications showed that stereospecific analyses of tria- chromatography. Overall, α-tocopherol was to several retinopathies. Sphingosine-1- cylglycerols together with other chemical the most responsive to the factors evaluated; phosphate can prevent this death but also determinations can be useful in building a the response of other tocopherols was often promotes cell proliferation that might lead to protocol for the evaluation of the genuine- lower or inconsistent across environments. neovascularization and fibrosis. Recent data ness of pumpkin seed oil from Slovenia. The seeding rate affected α-tocopherol con- support Cer and Sph as crucial mediators centrations in three out of five environments; in the induction of photoreceptor apoptosis Functional properties of select seeding at a rate of 40 seeds m−2 resulted in in diverse models of oxidative damage and 4% higher concentrations than seeding at a neurodegeneration and suggest that regulat- edible oilseed proteins higher rate. Wide row spacing (more than ing their metabolism can prevent this death. Sharma, G.M., et al., J. Agric. Food Chem. 36 cm) resulted, in two out of five environ- New evidence proposes a central role for 58:5457–5464, 2010. ments, in 6% higher α-tocopherol concentra- S1P controlling photoreceptor survival and Borate saline buffer (0.1 M, pH 8.45) tions as compared to narrower row spacing. differentiation. Finally, this review discusses solubilized proteins from almond, Brazil The seeding date had a greater impact; mid- the ability of trophic factors to regulate nut, cashew nut, hazelnut, macadamia, pine to late-May seeding across four environ- sphingolipid metabolism and transactivate nut, pistachio, Spanish peanut, Virginia ments resulted in 45% greater α-tocopherol S1P signaling pathways to control survival peanut, and soybean seeds were prepared concentrations than seeding at later dates. and development in retina photoreceptors. from the corresponding defatted flour. The Phosphorus and K fertilization had a negli- yield ranged from 10.6% (macadamia) to gible impact on tocopherol concentrations. Glycerophospholipid and 27.4% (almond). The protein content, on a Across experiments, large differences were dry weight basis, of the lyophilized prepa- observed between environments; plants triacylglycerol distribution in rations ranged from 69.23% (pine nut) to grown in northern environments consis- corn kernels (Zea mays L.) 94.80% (soybean). Isolated proteins from tently had lower concentrations of α- and Harrabi, S., et al., J. Cereal Sci., 51:1–6, Brazil nut had the lightest and hazelnut the γ-tocopherols but higher concentrations of 2010 darkest color. Isolated proteins exhibited δ-tocopherol. Differences between culti- The distribution of various molecular good solubility in aqueous media. Foaming vars were also consistent, ranging between species of triacylglycerols (TAG) and phos- capacity (<40% overrun) and stability (<1 10 and 30%, depending on the tocopherol. pholipids among three corn kernel parts was h) of the isolated proteins were poor to fair. Results demonstrate that soybean tocopherol determined by liquid chromatography/elec- Almond proteins had the highest viscos- concentrations are affected by crop manage- trospray ionization-mass spectroscopy. A ity among the tested proteins. Oil-holding ment and thus suggest that specific recom- comparison between three corn kernel parts capacity of the isolated proteins ranged from mended agronomic practices may need to be demonstrated that there was a s
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