Ethidium BromideAcridine Orange

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					            Ethidium Bromide/Acridine Orange

For discrimination of live from dead cells on the basis of membrane
•Acridine orange fluoresces GREEN ->LIVE cells.
•Ethidium bromide fluoresces ORANGE (intercalates with DNA) -> DEAD

From powder:
15 mg acridine orange
50 mg ethidium bromide dissolved in 1 ml 95% ethanol
add 49 ml H20, mix well, freeze in 1 ml aliquots.

Working conc for cell viabilities:
Take 1 ml & make up to 100 ml in PBS

From solutions:

300µL of 10 mg/mL acridine orange
1000µL of 10 mg/mL ethidium bromide
Make up to 100mL with PBS