Ovine Abortion DX Table OVINE ABORTION DIAGNOSIS Agent Specimen Placenta

							                                                                                                                             2005


                                          OVINE ABORTION DIAGNOSIS
                                     Washington Animal Disease Diagnostic Laboratory


               Agent                                             Specimen                                      Assay
     Border Disease pestivirus                                 Fixed fetal tissues                                H
                                                            Fresh spleen, lung, liver                             VI
                                                           Paired maternal sera (RT)                               S
         Bluetongue Virus                               Fixed fetal tissues and placenta                          H
                                                 Fresh spleen and brain, maternal blood (PT)                   VI, PCR
                                                           Paired maternal sera (RT)                               S
         Toxoplasma gondii                       Fixed Placenta (No consistent fetal lesions)                  H, IHC
                                                  Fetal fluids and Paired maternal sera (RT)                       S
          Coxiella burnetti                      Fixed Placenta (No consistent fetal lesions)                  H, IHC
         Chlamydia psittaci                      Fixed Placenta (No consistent fetal lesions)                  H, IHC
                                                                 Fresh placenta                               Ag ELISA
                                                           Paired maternal sera (RT)                               S
           Leptospirosis                              Fixed fetal kidney, lung, placenta                       H, IHC
                                                              Fetal thoracic fluid                              FA
                                                                  Fetal kidney                                  FA
                                                          Paired maternal sera (RT)                               S
      Listeria monocytogenes                                   Fixed fetal tissues                             H, IHC
                                                  Fresh fetal liver, spleen, stomach contents                  Culture
            Brucella ovis                              Fixed fetal tissues and placenta                           H
                                             Fresh stomach contents, liver, lung, spleen, placenta             Culture
                                                                Maternal sera ✼                                   S
Campylobacter fetus var. fetus and C.                  Fixed placenta and fetal tissues                           H
              jejuni
                                              Fresh fetal fluids, stomach contents, placenta, liver            Culture
              Mycoses                                   Fixed placenta and fetal tissues                         H
    (Aspergillus, Absidia, Mucor,         Fresh placenta and fetal tissues (placenta,stomach contents,         Culture
              Rhizopus)                                               lung)
           Toxic Plants                                                                                           T
       Veratrum californicum                                       Cyclopia

       Astragalus (locoweed)                              No consistent fetal lesions

      Nitrate containing plants                 Feed, fetal ocular fluid and abomasal contents

    ✼ Serology is the preferred method for specific diagnosis of brucellosis.




          RT-Red top tube; PT-purple top tube; VI-Virus isolation; PCR–Polymerase chain reaction; IHC-Immunohistochemistry
                             S-Serology; FA-fluorescence antibody test; H-Histopathology; T-Toxicology