THE CHARACTERIZATION OF 4 TIBETAN ARTEMIA STRAINS AND THE

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THE CHARACTERIZATION OF 4 TIBETAN ARTEMIA STRAINS AND THE Powered By Docstoc
					THE CHARACTERIZATION OF 4
TIBETAN ARTEMIA STRAINS AND
THE HUFA PROFILE DURING THE
ENRICHMENT AND STARVATION



    Xiuling Yu, Naihong Xin, Juju Sun
          Salt Research Institute
Characterization of 4 Artemia strains

 1. Biometrics:
 2. Hatching rate
 3. Fatty acid profile
   4 Tibetan Salt Lakes



1. Gaize lake (GZ)
2. Shuanghu Lake (SH)
3. Nima Lake (NM)
4.Yangnapengco Lake (YP).
    Table 1. Location, elevation and surface area of
                    4 Tibetan lakes

   Lake     Location         Elevation (m)   Area (km2)

   GZ      8405E3202N        4490        86
   YP           /                /           12
   SH       9110E3113N      4664         140
   NM       8607E3028N      4663         25
  Table 2 Biometrics of Artemia from 4 Tibetan
                     lakes
 Lake    Diameter of    Diameter of Chorion Dry weight Length Width
        hydrated cysts decapsulated ( μm) of nauplii of nauplii of nauplii
           ( m)       cysts( m)             (μg)      (μm)      (μm)
                                           a
  GZ    358.7818.24a 338.7318.42 a 10.02   8.2098 a  663.06 a 264.55 a

                                           a
  YP    291.0318.17b 265.8515.73 b 12.59     2.7310 b   527.69 b   204.78 b

                                           a
  SH    305.3116.86 b 289.7315.89 b 7.79     3.3650 b   544.35 b   225.80 b

                                           a
 NM     301.5820.04 b 283.7316.24 b 8.95     3.5682 b   560.91 b   22.88 b


Note: within the same column values sharing the same superscript are not
significantly different (p<0.05)
Table 3 Hatching rate of Artemia cysts
  from 4 Tibetan lakes at 28 C (hrs)
Lake    T10      T50      T90      TS
GZ      22      24       28       6
YP      18      23       27       9
SH      17      22       25       8
NM      17      18.5     23       6
Table 4 HUFA content of decapsulated
cysts from 4 Tibetan lakes (mg/g,D.W.)

 Fatty acid    GZ      YP      SH     NM

 C18:33      8.54    10.15   12.58   7.11

 C20:46      5.08    2.89    2.43    3.47

 C20:53      46.64   19.22   29.79   26.65

 C22:63      0.06    0.12     0       0
     Enrichment and Starvation
Two Artemia strains: 1. Great Salt Lake(EG)
                    2.Laguoco Lake (AT)

    Three enrichment diets: 1. DC Selco
                            2. DC Selco
                            3. A1 Selco
                 Conditions:

   Enrichment:800ml 35ppt seawater, 27C,
          density 300/ml(EG), 200/ml(AT),
             0.6g/l (two ration)

   Starvation: 800ml 35ppt seawater, 12 C,
       Density 900ml/(EG),600/ml(AT),
The samples were taken at 6 hrs,12 hrs and 24hrs
Table 5 The HUFA content (mg/g, D.W)
of A.franciscana (EG) and A.tibetiana
after 24 hours’ enrichment and during
starvation at 12 C.
Species Enrichment     Instar 1    24E    6h/12°C 12h/12°C 24h/12°C
    A1 Selco   ARA       1.83      3.68     4.51     4.17    3.69
               EPA       3.95     15.32    18.91    17.61    16.56
               DHA         0      21.72    23.06     20.6    17.77
             DHA/EPA       0       1.42     1.22     1.17    1.07
    DC Super   ARA       1.83      3.72     3.56     3.54    3.30
 EG  Selco     EPA       3.95     34.29    33.26    32.57    29.89
               DHA         0      12.32    10.63     9.82    7.80
             DHA/EPA       0       0.36     0.32     0.30    0.26
    DC Selco   ARA       1.83      4.13     5.06     5.09
               EPA       3.95     45.16    51.59    53.55
               DHA         0      25.81    24.98    23.92
             DHA/EPA       0       0.48     0.48     0.45
    A1 Selco   ARA       6.63      6.76     6.47     5.99
               EPA      52.54     50.66    47.93    45.08
               DHA       0.61      4.2      3.63     3.26
             DHA/EPA     0.01      0.08     0.08     0.07
    DC Super   ARA       6.63      8.64     7.54     6.53
 AT  Selco     EPA      52.54      75.1    65.97    56.73
               DHA       0.61      9.08     8.28     6.07
             DHA/EPA     0.01      0.12     0.13     0.11
      DC       ARA       6.63      8.09     7.36     6.88
     Selco     EPA      52.54     70.03    63.06    59.46
               DHA       0.61      8.97     7.87     7.18
             DHA/EPA     0.01      0.13     0.12     0.12
Note:
24E:      after 24 hours enrichment,
6h/12 C : store the enriched nauplii at 12C
           for 6 hours,
12h/12 C : store the enriched nauplii at 12
            C for12 hours,
24h/12 C : store the enriched nauplii at 12
            C for 24 hours
                Figure 1, HUFA content of A. franciscana nauplii enriched by
                            different enrichment diets for 24hours

           80
           70
           60
mg/g d.w




           50                                                                  ARA
           40                                                                  EPA
           30                                                                  DHA
           20
           10
            0
                   Newly         DC Selco       DC Super       A1 Selco
                  hatched                         Selco
                Figure 2, HUFA content of A.tibetiana enriched by different
                              enrichment diets for 24 hours

           80
           70
           60
mg/g d.w




           50                                                                 ARA
           40                                                                 EPA
           30                                                                 DHA
           20
           10
            0
                 Newly hatched    DC Selco      DC Super       A1 Selco
                                                  Selco
        The metabolism of A.franciscana(EG) and
A.tibetiana(AT) nauplii enriched with Super Selco during
                  cold storage at 12C

           80
           70
           60                                    ARA/EG
mg/g d.w




           50                                    EPA/EG
                                                 DHA/EG
           40
                                                 ARA/AT
           30                                    EPA/AT
           20                                    DHA/AT
           10
            0
                24E   6H/12C   12H/12C 24H/12C
      The metabolism of A.franciscana(EG) and
A.tibetiana(AT) nauplii enriched with A1 Selco during
                 cold storage at 12C
           80
           70
           60                                    ARA/EG
                                                 EPA/EG
mg/g d.w




           50
                                                 DHA/EG
           40
                                                 ARA/AT
           30                                    EPA/AT
           20                                    DHA/AT
           10
            0
                24E   6H/12C   12H/12C 24H/12C
       The decrease of DHA/EPA ratio of
 A.franciscana(EG) and A.tibetiana(AT) nauplii
   enriched with Super Selco during the cold
               storage at 12C%
 0.4

0.35

 0.3

0.25
                                                 EG
 0.2
                                                 AT
0.15

 0.1

0.05

   0
        24E      6H/12C    12H/12C   24H/12C
         The decrease of DHA/EPA ratio of
   A.franciscana(EG) and A.tibetiana(AT) nauplii
    enriched A1Selco during the cold stored at
1.6
                      12C
1.4

1.2

  1
                                                   EG
0.8
                                                   AT
0.6

0.4

0.2

  0
       24E       6H/12C    12H/12C   24H/12C