Camelina Product Development Program

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					Camelina Product Development Program

An Alternative Energy Crop for

                                        Biodiesel and Other Specialty Oil Uses

                             Sustainable Oils, LLC                           1
Sustainable Oils - Business Structure

      Joint Venture between Targeted Growth, Inc. and Green Earth Fuels
      LLC formed November 2007.
      Purpose: development and commercialization dedicated energy crops
       –   Camelina is the first crop
       –   others to follow

      Vertically integrated approach:
       –   Develop and produce proprietary seed and genetics

       –   Produce commodity grain through grower partnerships
       –   Market commodity oil and meal

                                        Sustainable Oils, LLC             2
Camelina - Characteristics

 Member of Brassicaceae
 – Related to Arabidopsis, Canola, etc.
 Low input crop
 – Adapted to marginal conditions
 – Northern U.S. and Southern Canada
 Oil producing crop
 – Seed Oil in the Range of 35-38%
 – Fatty acid composition high in

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Camelina - Long Term Development Program

  Breeding program initiated in 2005
  Three-Pronged Strategy for Germplasm Enhancement:
   – classical and molecular breeding
   – mutation breeding
   – transgenic
  Increased yield (huge potential for improvement)
  Improved fatty acid composition
   – 18:1 is “optimal” fatty acid for biodiesel

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Approach 1 - Classical and Molecular Breeding

  Initiated broad field evaluation
  of >90 accessions in Northern
  United States and Canada in
   – seed yield
   – oil yield
   – fatty acid composition
   – agronomic traits
  Molecular marker breeding
  approach in 2007
   – relatedness of available cultivars
   – closely related wild species
   – developing proprietary markers
     to enhance product development
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Evaluation Field Trials

Trials throughout northern
U.S. and S. Canada

 – 20 in 2007
 – ≈ 40 in 2008

Preliminary identification of
higher yielding, superior

Evaluation and selection of
F2-F5 families and lines in
multiple nurseries
                                                  2008 Field Trial and Nursery Locations
Breeder, Foundation and
Commercial seed increases

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Approach 2 - Mutation Breeding

EMS mutagenized population
created in a selected Camelina
line in 2007
 – General trait screening
Specific Targets include
 – Altered fatty acid compositions
 – Improved yield

                                                     M1 Population, ~ 10,000 lines

                             Sustainable Oils, LLC                                   7
 Mutation Breeding – Current Status

TILLING population developed;
mutation frequency determined

Gene sequences for select targets
isolated and characterized

M3 seed harvested in March 2008
                                                    M2 Population, ~ 8,500 lines
Identification of lines with desired
phenotype (s) underway

                                Sustainable Oils, LLC                              8
Approach 3 - Transgenic
   TGI’s REV and KRP yield enhancement technology
   Proprietary transformation developed in 2006
    – Efficient transformation for multiple cultivars
    – Multiple constructs undergoing greenhouse and field
   Replicated multi-location field trials in 2008 in U.S. and Canada

                            Sustainable Oils, LLC                      9
Thank You

Sustainable Oils, LLC   10