BIND Newsletter March Issue No Welcome to the first by galenbarbour


									                                                               BIND® Newsletter | March 20, 2008 | Issue No. 1

Welcome to the first issue of the BIND® Newsletter. We will be sending these periodically in the future to update you
on new label-free products and applications.

In this issue:

      ●   Product News
      ●   New Applications
      ●   Upcoming Events (Registration Savings for SBS St Louis)
      ●   Contact Us

Product News

SRU Biosystems installs its BIND® Reader For Label-free Drug Profiling using Primary Cells at GlaxoSmithKline
(3/20/08) more

New CA-1 BIND® Biosensor for Cell Based Assays
SRU Biosystems is introducing the CA-1 BIND Biosensor at SBS in St. Louis this year. The biosensor was developed for
enhanced performance of both adherent and suspension cell types. This biosensor will be available in both 96-and 384-
well format.

Development of Novel BIND® Biosensor surfaces for Cell Based Assays
by Lance Laing Ph.D. , Director of Bioapplications, SRU Biosystems

Label-free optical biosensors are unlike any screening technology currently employed because they rapidly measure
changes in cell adhesion and morphology of live cells in real time. This makes attachment of the cell to the sensor and
neighboring cells critical. If the attachment of the cells to the sensor is too tight or has too many points of contact this
restricts the response of the cells to perturbation resulting in a low dynamic range. If there is too little attachment the cells
are easily disturbed or removed during washing or liquid additions. Additional coatings and protocols will be made
available in the future for primary and stem cell applications.
HEK cell adherence to BIND® TiO Biosensors. HEK cells were adhered to either serum-coated biosensors at cell
concentrations of 0, 32K, 16K or 8K cells per 384-well, or to CA-1 coated biosensors (Graph A). Cells on CA-1 coating
withstood advanced washing protocols compared to serum coating in subsequent steps (Graph B).

New Applications

Endogenous Chemokine Signaling in Suspension Cells

Utilizing the advanced CA-1 BIND Biosensors, Human monocytic leukemia THP-1 cells were tested for their ability to be
immobilized on the sensors and evaluated for their response to endogenous chemokine signaling.

Use of suspension cells on BIND® : THP-1 cell activation with chemokines THP-1 cells were seeded into a 384-well
BIND Biosensor coated with a proprietary suspension cell binding surface, CA-1. After 2 hour starvation, chemokine
mediated THP-1cell response was monitored over time. Prior to injection of chemokine a stable baseline is obtained.
Kinetic profiles of rapid cell response is possible with BIND. MCP maximum response peaks in 10 minutes, then rapidly
declines. MIP-1α and Rantes also reach maximum response rapidly but then remain stable over 60 minutes.
Upcoming Events

SBS Events – Visit us at Booth #301

Society for Biomolecular Sciences 14th Annual Conference & Exhibition
St. Louis, MO, April 6-10, 2008

                         As a Bronze Sponsor at the 2008 SBS Conference SRU Biosystems is able to pass on a
                         substantial saving on registration- as much as $700! (until March 25th). Download the
                         coupon here.
                         We hope to see you in St Louis and welcome you at booth #301

        SBS Short Course: Plate Based Label-Free Biosensors for Drug
        Discovery: A Whole New World
        Sunday, April 6, 2008
        8:30am – 5:00pm

        Tutorial: The BIND® Reader: A Universal Platform for Label-Free Drug Discovery
        Presenters: Dr. Julio Martin, GlaxoSmithKline, Dr. Kathryn Dodgson, AstraZeneca
        & Dr. Lance Laing, SRU Biosystems
        Monday, April 7- 9:00am-9:45am

        Presentation: Microplate-Based High Resolution Label-Free HCS for Application
        to Native Cell Lines & Pharmaceutically Relevant Targets
        Presenter: Dr. Lance Laing, SRU Biosystems
        Wednesday, April 9, 2008
        10:50am - 11:15am in Session: Label-Free Detection in HTS & Lead Validation

Download a coupon with savings of up to $700 on registration until March 25th. Go to our website homepage under
events to download the Savings Coupon.

Contact Us

For more information or to schedule a technical presentation contact

Mann Shoffner
Director of Sales and Marketing
781-933-7255 (phone)
617-823-2988 (cell)

About The BIND® System

BIND® from SRU Biosystems is a universal assay system that enables label-free detection of drug-target interactions. The
system is comprised of the BIND® Reader and 96- or 384-well microplate biosensors. The BIND system uses a novel
optical effect to provide very sensitive measurements of changes in binding or adherence in the proximity of the biosensor
surface. The biosensor incorporates a proprietary nanostructured surface, and is incorporated in microwell plates in
industry standard formats. The BIND system delivers higher quality assay data and improved throughput, both resulting in
cost savings.

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