DIFFERENCE DIAC NEWSLETTER A Newsletter of the European Research
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DIFFERENCE/DIAC NEWSLETTER A Newsletter of the European Research projects ‘Dioxins in Food and Feed – Reference Methods and New Certified Reference Materials’ (DIFFERENCE) and ‘Dioxin Analysis by Comprehensive Multi-dimensional Gas Chromatography’ (DIAC). No. 2 – 12 December 2002 The two European showed that a separation DIFFERENCE research projects DIAC of 90 PCBs and 17 and DIFFERENCE have relevant dioxins and furans Also in DIFFERENCE the made good progress since in one GCxGC/ECD run focus has been on the their start at 1 October (148 min) is possible. development of methods. 2001 and 1 February Shorter run times are Extraction methods have 2002, respectively. This expected to be achievable. been tested by the Lund newsletter gives a brief A large number of samples University and the Joint overview of the has been prepared by the Research Centre, Ispra. achievements so far. Institute Quimic de Sarria Accelerated solvent (IQS) in Barcelona, to be extraction (ASE) appears DIAC used in the quantitative to be very suitable for evaluation of the GCxGC dioxin/PCB analysis. A cell The DIAC project has technique and comparison size of 100 ml will normally seen its third meeting of with GC/HRMS. Mrs. dr. be required unless the project group, at 8 Carmen Rodriguez of IQS samples are highly November in Umeå, has meanwhile been contaminated. Varying Sweden. The second replaced by dr. Jordi Diaz. recoveries were obtained meeting was held at 10 New software for by microwave assisted April in Bordeaux, France. integration of GCxGC extraction (MAE). Good The focus of the DIAC chromatograms is now results were obtained for project so far has been on gradually coming available GC-LRMS/MS analysis tests of the various and will support the (University of Barcelona). modulator types and GC quantitative evaluation of This technique, as well as column combinations. The this technique. the CALUX bio-assay and comparison of the the GCxGC technique are modulators showed clearly that cryogenic modulators are preferred to thermal modulators, because they provide peaks with smaller widths. A few good cryogenic modulators are available, and the selection of these may depend on details. A number of column combinations was tested. Until now the DB1-HT8, DBXLB-LC50 and HT5- BPX50 appear to offer the best resolution. One article has appeared in J. Chromatogr. A, which Third DIAC meeting in snowy Umeå (Sweden) now ready to start the for homogeneity and analysis, bio-assays and quantitative evaluation. stability. The pre- dioxin/PCB interlaboratory Additional tests will be certification of these studies. The task of this carried out by RIKILT, materials is planned for expert group will be to Wageningen on large 2003/2004. review the progress of volume injection in both projects and to combination with LRMS. provide critical comments The DELFIA technique External Expert Panel and suggestions for which was initially included improvements with regard in this project has now In consult with EU to the completion of the been replaced by an Ah- Measurements and projects and PCR technique, which will Testing it has been implementation of the be tested by the Joint decided to install one developed techniques in Research Centre in Ispra external expert panel for European dioxin (shortly expected to move both projects. We are laboratories. The expert to Geel, Belgium). Based proud to introduce the group will meet for the first on the results obtained so members of this external time at 13 February 2003 far, the following expert group: dr. Frans in Brussels. combinations will now be Verstraete, European tested on their quantitative Commission, Health and Web site performance: GC/HRMS Consumer Protection DG, (reference), CALUX bio- Brussels, Belgium, dr. The address of the assay, Ah-PCR technique, Rainer Malisch, State DIFFERENCE/DIAC GC/LRMS/MS, GCxGC, Institute for Chemical and website, which has been ASE-CALUX, and ASE- Veterinary Analysis of frequently visited until now GC/HRMS. A large Food, Freiburg, Germany, by scientists from all over number of samples has dr. Peter Behnish, SGS the world, is been prepared by RIVO Controll, Institute for www.dioxins.nl. for the test series. These Environmental Protection samples include pork, Services, Wismar, Questions? milk, fish, fish oil, Germany, dr. Bert van compound feed and Bavel, Örebro University, For specific questions, standard solutions. The Örebro, Sweden. The comments, suggestions, tests will be carried out in expertise of this group etc., please email to: three rounds between includes knowledge on the firstname.lastname@example.org- November 2002 and application of the ur.nl (for DIFFERENCE), October 2003. In addition, developed techniques in and candidate certified relation with the new email@example.com- reference materials are European MRLs, analytical ur.nl (for DIAC). being prepared and tested chemistry for dioxin/PCB 6.0 Hx-CDD This newsletter is scheduled to & appear once per half year Hx-CDF 5.0 Pe-CDD & Pe-CDF 2nd dimension retention time [s] 4.0 T-CDD & T-CDF OCDD 3.0 & Hp-CDD OCDF & 2.0 Hp-CDF 1.0 0.0 75 85 95 105 115 125 135 145 1st dimension retention time [min] Separation of a mixture of 90 PCBs (partly visible) and 17 WHO dioxins and furans by comprehensive GCxGC/ECD.