^sup 1^H NMR-based protocol for the detection of adulterations of refined olive oil with refined hazelnut oil

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^sup 1^H NMR-based protocol for the detection of adulterations of refined olive oil with refined hazelnut oil Powered By Docstoc
					             inform February 2010, Vol. 21 (2) 111

        H NMR-based protocol for the                   spectrometers operating at different mag-           Primary outcomes were fecal cholesterol
                                                       netic fields, that is, at proton frequencies of     excretion and intestinal cholesterol absorp-
       detection of adulterations of                   500 and 400 MHz, were investigated.                 tion measured with stable-isotope tracers
       refined olive oil with refined                                                                      and serum lipoprotein concentrations. Phy-
       hazelnut oil                                    Dose effects of dietary                             tosterol intakes (diet plus supplements)
       Mannina, L., et al., J. Agric. Food Chem.       phytosterols on cholesterol                         averaged 59, 459, and 2,059 mg/d during
                                                                                                           the three diet periods. Relative to the 59-mg
       57:11550–11556, 2009.                           metabolism: A controlled                            diet, the 459- and 2,059-mg phytosterol
            A 1 H nuclear magnetic resonance
       (NMR) analytical protocol for the detec-
                                                       feeding study                                       intakes significantly (P < 0.01) increased
       tion of refined hazelnut oils in admixtures     Racette, S.B., et al., Am. J. Clin. Nutr.           total fecal cholesterol excretion (36 ± 6%
       with refined olive oils is reported accord-     91:32–38, 2010.                                     and 74 ± 10%, respectively) and biliary cho-
       ing to ISO format. The main purpose of               Phytosterol supplementation of 2 g/d is        lesterol excretion (38 ± 7% and 77 ± 12%,
                                                       recommended by the National Cholesterol             respectively) and reduced percentage intes-
       this research activity is to suggest a novel
                                                       Education Program to reduce LDL (low                tinal cholesterol absorption (–10 ± 1% and
       analytical methodology easily usable by
                                                       density lipoprotein) cholesterol. However,          –25 ± 3%, respectively). Serum LDL cho-
       operators with a basic knowledge of NMR
                                                       the effects of different intakes of phytosterol     lesterol declined significantly only with the
       spectroscopy. The protocol, developed on
                                                       on cholesterol metabolism are uncertain.            highest phytosterol dose (–8.9 ± 2.3%); a
       92 oil samples of different origins within      We evaluated the effects of three phytos-
       the European MEDEO project, is based on                                                             trend was observed with the 459-mg/d dose
                                                       terol intakes on whole-body cholesterol
         H NMR measurements combined with a                                                                (–5.0 ± 2.1%; P = 0.077). Dietary phytos-
                                                       metabolism. In this placebo-controlle
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