Sodium Bicarbonate Buffer NaCl 0 1 M Bicarbonate Buffer 0 by hcj

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									0.1 M Bicarbonate Buffer, 0.5 M NaCl, pH 8.3
(used for coupling proteins to CNBr-activated sepharose)
Kent Hill
3-10-00


0.1 M Na2CO3 , 0.5M NaCl
10.6 g Na2CO3 (105.99 g/mol)
29.2 g NaCl
QS 1 ltr H2O
(pH = 8.07)


0.1 M NaHCO3 , 0.5 M NaCl
8.4 g NaHCO3 (84.11 g/mol)
29.2 g NaCl
QS 1 ltr H2O
(pH = 11)




Take 200 ml of NaHCO3 and titrate to pH 8.3 with Na 2CO3 (approx. 4.0 ml)

								
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