LSM 510 Laser Scanning Microscope by xqo30826


									         LSM 510
Laser Scanning Microscope

             LSM 510 – flexible...
             The confocal LSM 510 Laser Scanning
             Microscope puts you in command of
             advanced, flexible technology.
             The compact scanning module can be
             configured in multiple ways to match a
             wide variety of specimens and applications.

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                      Legends to illustrations see last page.
LSM 510 Laser Scanning Microscope – The Synthesis of Experience
The LSM 510 from the pioneer of laser           Confocal – compact – versatile                        Brilliant, laser-sharp images
scanning microscopy. The natural result of      Superbly engineered, here is system inte-             The LSM 510 resolves no less than
150 years of innovation in optics and more      gration at its best: Transferring the                 2048 x 2048 pixels – more than four
than 12 years of experience in all relevant     compact scanning module from one micro-               million! Together with scanning fields of
fields of laser scanning microscopy.            scope to another takes only a few minutes.            unbeatable size, this allows you to image
The LSM 510 is the powerful combination         The researcher can use the LSM 510 on an              even large overview pictures without
of confocal microscopy and the fully auto-      inverted microscope in the morning and                losing any information.
mated research microscopes Axioplan 2           on an upright model in the afternoon, and             Four independent 12-bit analog-to-digital
and Axiovert – everything from Carl Zeiss.      achieve excellent results with both.                  converters providing up to 4096 brightness
                                                To ensure this, it takes more than a few              levels give you an ample range of control
                                                pinholes. The quality and resolving power             and variation to optimize a wide range
                                                of confocal imaging depend on many                    of images.
                                                factors. We have designed a new optical
                                                train and recomputed every detail, from
                                                the microscope to the ICS objectives to the
                                                scanner and pinhole optics, to ensure                 The freedom you need
                                                optimized performance.                                To investigate your multi-fluorescence
                                                                                                      specimens, the LSM 510 offers four simul-
                                                                                                      taneous confocal channels for reflection
                                                                                                      and fluorescence, plus an extra channel
                                                                                                      for transmitted-light examination. Each of
                                                                                                      the four channels can be separately
                                                                                                      controlled, with all degrees of freedom for
                                                                                                      pinhole adjustment. Alternatively, you may
                                                                                                      have the computer optimize everything
                                                                                                      for your specific application. This indi-
                                                                                                      vidual freedom provides the flexibility you
                                                                                                      need for your research.
    The confocal                                                                                      The LSM 510 lets you effortlessly handle
    beam path                                                                                         multi-labeling images with extremely
    principle                                                                                         differing brightnesses, and reproduce
                                                                                                      them at any time with a mere mouse click.

                                      Human synovial cells, 4 confocal fluorescence channels, nuclear structures (DAPI, blue), microtubuli (CY5, red),
                                                                          actin filaments (FITC, green), nucleoli (Texas Red, white) (Dr. Albini, Boston)
ce and Innovation.

Modular flexibility
Mounted directly below the inverted
Axiovert 100 M BP (base port) microscope
stand, the scanning module does not
impede your free access to the micro-
scope. Moreover, it ensures maximum light
yield for your photon-hungry applications.
On the Axiovert 100 M SP (side port), the
scanning module fits to a lateral port.
This facilitates fast changeover if you
want to use the same scanning module
on the upright Axioplan 2 as well.

Controlled complexity                           Simplicity at two levels                       Impressive package
The LSM 510 comes with tailor-made              Confocal microscopy has its own laws.          of advanced features
software for full system control. With all      Whether you are an expert or a novice,         Setting the desired laser line and the
controls motorized, fast method changes         or working in multiple use environments,       necessary brightness on the LSM 510 is
and automatic operation are exceptionally       it's no problem: the LSM 510 will give you     performed within microseconds, and
easy. To reproduce results at any time,         expert results. This is because the software   without any moving parts. The result is
day after day, all you need to do is to press   offers you two levels of operation.            monitored by a reference photodiode,
a button.                                       The easy-to-use operator interface guides      which you can use as a sixth channel.
In multi-user operation, every user can         you to the correct results with a minimum      This is ideal for morphology and physio-
save and reload their own setting para-         of instructions and graphical user prompt-     logy, for experiments such as ratio imaging,
meters or complex examination proce-            ing with automatic setting of many para-       release of caged compounds, or FRAP.
dures and thus have their own individual        meters for day-to-day routine. Simply          Digital control enables entirely new,
research tool in one and the same setup,        follow the system's recommendations, or        sophisticated scanning techniques. The
without disturbing other user settings.         overrule them manually as your specimens       available spectral range starts with UV, for
                                                require.                                       work in the life sciences, and ends with IR,
                                                The expert level below the operator            for multi-photon microscopy. Other fea-
                                                interface provides in-depth control –          tures include automatic Z aberration adjust-
                                                the perfect tool for individual settings of    ment for all colors by collimator optics,
                                                functions and parameters for the more          top-grade photomultipliers for fluorescence
                                                advanced user.                                 and transmitted light, external channels
                                                                                               and many more, which add up to per-
                                                                                               fection down to the last detail.

                                                                                               HRZ 400 Motorized Galvanometric Z-Stage

Flexible presentation of images                                                                Graphical user prompting
... with no compromise
Forget about “ORs” in laser scanning
microscopy. From now on, it's only “AND”.
Modular AND confocal AND upgradeable
AND inverted AND upright.
High-tech sophistication AND flexibility.
Compact design providing stability and
shortest light paths, optical precision,
innovative technology and ingenious
scanning techniques combine to produce
perfect 3D images of all specimen

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                                                        Legends to illustrations see last page.
LSM 510 Laser Scanning Microscope
 Microscopes                       Inverted Axiovert 100 M BP or SP                                           VIS laser                         Visible light fiber coupling, single mode,
                                   Upright Axioplan 2 MOT                                                     module                            polarization preserving
                                   Fixed stage Axioskop 2 FS MOT                                                                                Laser beam attenuation for all visible light
                                   All accessories (ICS objectives, filters etc.)                                                               lasers by a single temperature
                                   can be used.                                                                                                 stabilized AOTF
         Z motor                   DC servomotor, optoelectronic coding,                                                                        HeNe laser (543 nm, 1 mW)
                                   stepping size 50 nm                                                                                          HeNe laser (633 nm, 5 mW)
         HRZ-200                   Galvanometric stage of extra-high preci-                                                                     Ar laser (458, 488, 514 nm, 25 mW)
                                   sion, focusing range 200 µm, step size                                                                       Ar laser (488 nm, 15 mW)
                                   7 nm, accuracy 40 nm, permitting                                                                             ArKr laser (488 nm, 568 nm, 30 mW)
                                   continuous Z scan with up to 10 Hz                                         UV laser                          Single mode,
                                                                                                              module                            polarization preserving
         XY scanning               Motorized, range 100 mm x 100 mm,                                                                            laser beam attenuation for all lasers
         stage                     smallest step size 0.25 µm                                                                                   by UV-AOTF, temperature stabilized
 Scanning                          2 independently operating galvanometric                                                                      KrAr laser (351 nm, 364 nm, 80 mW)
 module                            mirrors
                                                                                                              Electronics                       Control circuitry for microscope,
         Resolution    up to 2048 x 2048 pixels                                                               module                            scanning and laser modules
         Zoom          1x ... 8x, infinitely variable                                                         Computer                          High-end INTEL® computer, ample RAM
         Field size    up to 18 mm (1x zoom,                                                                                                    space, extra-large hard disk, high-reso-
                       Plan-Neofluar 1.25x/0.04 objective)                                                                                      lution true-color graphics card, large-
         Detectors     up to 4 channels simultaneously, 4 PMTs                                                                                  screen monitor, keyboard, mouse
                       for R/FL, 1 PMT for transmitted light,
                       monitor diode                                                                          Software                          Windows NT operating system: LSM
         Pinholes      1 variable pinhole each for up to                                                                                        software for controlling the microscope,
                       4 confocal channels                                                                                                      scanning and laser modules, image
         Dynamic range 12 bit A/D converter for each detection                                                                                  recording, and the analysis, presentation
                       channel                                                                                                                  and management of image data

                                                                                                                                                                      Development of the LSM 510
                                                                                                                                                                      in cooperation
                                                                                                                                                                      with EMBL Heidelberg

Legends to the illustration on page 2                                                                        Legends to the illustration on page 7
 2/1 Primary culture, Fura red     2/2 Fluorescence and           2/3 Drosophila embryo, early                7/1 Live colon cells, SNARF-1     7/2 Neuroglia cells in culture,     7/3 Membrane labelled
 and Fluo-3                        brightfield                    stage of development, double                (Dr. Montrose, JH University,     Astrocytes (FITC), Oligodendrocytes connective tissue cells,
 (“Imaris”, Bitplane AG, Zürich)   (Dr. Nitschke, Freiburg)       fluorescence, FITC (green) indicates        Baltimore)                        (Rhodamine), nuclei (DAPI), Dr. M.  3D projection
                                                                  Actin filaments, PI (red) shows                                               Bastmeyer, Uni Konstanz, (Dr. H.
                                                                  RNA. (Dr. E. Schejter, Tel Aviv, Israel)                                      Schaden, Carl Zeiss Jena GmbH)
 2/4 3T3 cell culture, TMRE,       2/5 Cytokeratins and           2/6 Cell walls and                          7/4 Mouse fibroblasts,            7/5 Mouse fibroblasts, actin      7/6 Fibroblast, microtubuli,
 Calcium Green                     desmoplacine                   chloroplasts, double                        cytoskeleton structures           filaments                         intermediary filaments,
 (Dr. Sparagna, UCT                (Dr. Kartenberg, Heidelberg)   fluorescence                                (Dr. Iwig, University of Halle)   (Dr. Iwig, University of Halle)   nucleoli

 2/7 Cell nuclei, cytokeratins     2/8 Drosophila embryo,         2/9 Tissue section, multiple                7/7 Mitotic cells, multiple       7/8 Cultured cells, multiple      7/9 Cell culture, 3D shadow projection
 and desmoplacine                  cytoskeleton                   fluorescence                                fluorescence (NCI Maryland)       fluorescence                      (calculated by “Imaris”, Bitplane AG,
 (Dr. Kartenberg, Heidelberg)      (Dr. Zhang, Duke University,   (M. Günthert, ETH Zürich)                                                     (Prof. Wunderli-Allenspach,       Zürich) showing tight junctions (red)
                                   Durham)                                                                                                      ETH Zürich)                       and cytoskeleton structures (green).
                                                                                                                                                                                  (Prof. Wunderli-Allenspach, ETH Zürich)

For further details, please contact:
                                                                                                                                                                                                                            Printed on environment-friendly paper, bleached without the use of chlorine.

                                        Carl Zeiss
                                        D-07740 Jena
                                        Phone: ++49-36 41/64 -1616
                                        Telefax: ++49-36 41/64 -3144

Subject to change                                                                                                                                                                                 40-050 e/09.99

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