Introducing a new instrument
to the Cancer Center Flow
Cytometry Core Facility:
The LSR II by Becton
BD LSR II
New technology in the BD
LSRII optics and digital
electronics have created a
more sensitive flow cytometer
that yields more information
from each sample.
The new Windows® based
digital acquisition system
provides increased detection
and channel resolution by
sampling signals at 10 million
times a second.
Up to 8-color detection
The LSRII is fully configurable with three fixed-aligned,
air cooled lasers.
The 488nm blue laser detects four wavelengths at once,
allowing for more versatile experimentation.
The optical system of the 633 HeNe red laser can detect
both APC and APC-Cy7 simultaneously.
A third 405nm violet laser
has two detectors, making
it capable to analyze
ECFP, Alexa Fluor 405,
Pacific blue or side
Current configurations allow for up
to 8-color analysis and in the future
can be upgraded for detection of up
to 18 colors!
Eight-color detection with many combinations
of fluorochromes and fluorescent proteins.
BD FACSDiva ™
Flow Cytometry Acquisition & Analysis Software
And Snap-To gating.
Automated compensation uses unstained and single
positive controls to determine proper compensation.
Once set, the software adjusts the compensation as
needed between experiments.
When manually compensating a sample, a user need
only take the data file and compensate later.
This is efficient when there are few cells to waste
compensating before the data can be acquired.
With a single click on a
population, the snap-to
gate will automatically
gate the population.
The gate will adjust as
the population moves or
when toggling between
Flow Cytometry Core Facility
To set up time for a Philip R. Streeter Ph.D.
demonstration, analysis or
training please contact Dr. email@example.com
Phil Streeter or Miranda (503)494-1762
Also, feel free to stop by
the facility in the VA firstname.lastname@example.org
Research Center Building (503)220-8262 x56874
103 room E147.