Introducing a new instrument to the Cancer Center Flow

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Introducing a new instrument to the Cancer Center Flow Powered By Docstoc
					Introducing a new instrument
  to the Cancer Center Flow
   Cytometry Core Facility:
     The LSR II by Becton
          Dickinson
BD LSR II
        New technology in the BD
         LSRII optics and digital
         electronics have created a
         more sensitive flow cytometer
         that yields more information
         from each sample.
        The new Windows® based
         digital acquisition system
         provides increased detection
         and channel resolution by
         sampling signals at 10 million
         times a second.
        Up to 8-color detection
   The LSRII is fully configurable with three fixed-aligned,
    air cooled lasers.
   The 488nm blue laser detects four wavelengths at once,
    allowing for more versatile experimentation.
   The optical system of the 633 HeNe red laser can detect
    both APC and APC-Cy7 simultaneously.
                                A third 405nm violet laser
                                 has two detectors, making
                                 it capable to analyze
                                 experiments including
                                 ECFP, Alexa Fluor 405,
                                 Pacific blue or side
                                 population.
       8-color Analysis!




   Current configurations allow for up
    to 8-color analysis and in the future
    can be upgraded for detection of up
    to 18 colors!
Eight-color detection with many combinations
  of fluorochromes and fluorescent proteins.
    BD FACSDiva                         ™

Flow Cytometry Acquisition & Analysis Software


                              Simplify operational
                              efficiency using:
                                PC-like browser,

                                Reusable acquisition
                                  and analysis
                                  templates,
                                Automated
                                  compensation setup,
                                And Snap-To gating.
      Automatic Compensation
   Automated compensation uses unstained and single
    positive controls to determine proper compensation.
   Once set, the software adjusts the compensation as
    needed between experiments.

         Offline compensation
   When manually compensating a sample, a user need
    only take the data file and compensate later.
   This is efficient when there are few cells to waste
    compensating before the data can be acquired.
Snap-To Gating
         With a single click on a
          population, the snap-to
          gate will automatically
          gate the population.
         The gate will adjust as
          the population moves or
          when toggling between
          files.
Flow Cytometry Core Facility
To set up time for a             Philip R. Streeter Ph.D.
demonstration, analysis or
training please contact Dr.       streetep@ohsu.edu
Phil Streeter or Miranda          (503)494-1762
Boyd.
                                 Miranda Boyd
Also, feel free to stop by
the facility in the VA            boydm@ohsu.edu
Research Center Building          (503)220-8262 x56874
103 room E147.