IMS01-133-332 Pseudomonas aeruginosa by tpf49254


									            product IMS01-133-332
            Pseudomonas aeruginosa

            product information
                        background     Pseudomonas aeruginosa is Gram-negative, aerobic, rod-shaped bacteria with
                                       unipolar motility. An opportunistic human pathogen, P. aeruginosa is also an
                                       opportunistic pathogen of plants. P. aeruginosa bacterias are clinically important
                                       because they are resistant to most antibiotics and they are capable of surviving in
                                       conditions that few other organisms can tolerate. Pseudomonas is often
                                       encountered in hospital and clinical work because it is a major cause of hospital
                                       acquired (nosocomal) infections. Its main targets are immunocompromised
                                       individuals, burn victims, and individuals on respirators or with precipitation
                                       procedure followed by indwelling catheters. Additionally, these pathogens
                                       colonize the lungs of cystic fibrosis patients. P. aeruginosa is often identified by its
                                       pearlescent appearance and grape-like odor in vitro. Definitive clinical
                                       identification of P. aeruginosa includes identifying the production of both
                                       pyocyanin and fluorescein as well as its ability to grow at 42°C.

                        immunogen      whole, formaldehyde killed, bacteria, strains PAO1 and Habs1

                    antibody format    hen; polyclonal; total IgY in 0.9% NaCl, 0.1% sodium azide;       liquid

                           quantity    200 µl

                            storage    store at 4°C; make aliquots to avoid working with a stock. Please, remember to
                                       spin tubes briefly prior to opening them to avoid any losses that might occur from
                                       liquid material adhering to the cap or sides of the tubes.

                 tested applications   Western blot (WB)

              additional information   the IgY fraction is isolated by a two-step PEG precipitation procedure followed by
                                       ammonium sulphate precipitation

            application information
             recommended dilution      1: 1000 - 1: 5 000 with standard ECL (WB), 1: 1000 - 1: 5 000 (ELISA)

                expected | apparent
                                       n. a.

                confirmed reactivity   Pseudomonas aeruginosa strains PAO1 and Habs1

                predicted reactivity   n. a.

                     not reactive in   no confirmed exceptions from predicted reactivity known in the moment

              additional information   reacts with P. aeruginosa strains PAO1 and Habs1; it will also crossreact with
                                       other P. aeruginosa strains in ELISA and immunoblotting

                selected references    to be added when available

06/25/10 05:42:42
06/25/10 05:42:42

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