Detection of Endocrine Disrupting compounds by a Tyrosinase Biosensor Endocrine disrupt compounds (EDCs) or environmental hormones are steroid-like compounds that exist in nature or are produced during industrial process. EDCs, once ingested, may interfere with the functions of normal endocrine system in both wildlife and humans, becoming a major concern world wide. Hence, the development of a quick and easy operated biosensing device is necessary. In this study, an electrochemical biosensor for the detection of endocrine disrupt compounds based on tyrosinase entrapped within gelatin film is designed and developed. The synthetic oestrogens and phenolic compounds, the suspected endocrine disrupt compounds, including bisphenol A, diethylstilbestrol (DES), paraben and 3-aminophenol are tested on the developed biosensor. Amperometric response of biosensor to EDCs in a phosphate buffer at pH 6 was measured as a function of concentrations at fixed bias voltage of −0.2 V versus Ag/AgCl.The electrode gives linear response range of 0.1 – 20 M, 10–100 M, 1–50 M and 20-100 M for bisphenol A, diethylstilbestrol (DES), 3-aminophenol and paraben , respectively. The detection limit for bisphenol A, diethylstilbestrol (DES), 3-aminophenol and paraben are, nM, nM, 20 nM and 5 M, respectively. Moreover, the developed biosensor exhibits a good reproducibility with a relative standard devision (R.S.D) of 5.8 %.
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