December 2005 SLTB Newsletter **STOP PRESS ** Please register for news about the preparation of CRYO 2006 under http://www.CRYO2006.org. More details inside. The SLTB would welcome applications for Audrey Smith Travel Awards to attend CRYO 2006 in Hamburg (24-27 July). Each bursary will be for 100GBP. Two groups of applicants will be preferentially supported, specifically; researchers new to the field (normally MSc, or PhD students, or researchers in their first post-doctoral appointment) and technical staff/support scientists for whom funding for meeting attendance is problematic. Deadline for applications 1st May 2006. Please visit the SLTB website http://www.sltb.info/ for details on how to apply. SLTB Scientific meeting and AGM This year the Society presented a truly University of York, Sept 15-16th international face with nearly 40% of the attendees coming from outside the UK. Thanks to all those SLTB members who Small meetings such as ours (38 supported the 2005 AGM. We are registrants this year) are very dependent particularly grateful to Maureen Wood, on industrial sponsorship for finance. We Dirk Hincha and Roger Pearce for their are therefore very grateful to our invaluable help in organizing the two exhibitors and sponsors (Asymptote Ltd, symposia, and to all those who contributed Planer plc, Thames Cryogenics Ltd, to the scientific programme in any way. Linkam Scientific Instruments and The sessions were highly enjoyable and Cryosafe Ltd) for their support, and to informative and generated lively Planers in particular for their generous discussion. The student presentations were sponsorship of the opening reception. also all of a high standard. This year’s Ken To conclude, I personally want to thank Hobbs student prize went to Ashraf Belinda Wade whose hard work and super Sandouka from Palestine who is currently organizational skills ensured that studying with Colin Green at the everything went smoothly and, more or Northwick Park Institute for Medical less, according to plan. Research in Harrow. The title of his talk was ‘Carbon-monoxide releasing All best wishes for SLTB 2006 molecules: a novel stratagem to improve Monica Wusteman cold preservation techniques for kidney Dept of Biology, University of York transplantation.’ I first became interested in cryobiology in New Committee Members the late 1980s when working with John Morris & Brian Grout at Cell Systems Ltd, Tiantian Zhang Cambridge. In December 1990 I took on (email@example.com) the role of Curator of CCAP at I am a professor in Cryobiology at the Windermere and became responsible for Institute of Research in the Applied cryobanking activities of this collection. Natural Sciences, University of Luton, Subsequently, I became actively involved UK. I obtained both my BSc (China) and in cryobiological research and this has MPhil (UK) degrees in Environmental included close collaborations with Erica Biology. My PhD study was on Benson’s group at Abertay. I continue ‘Investigations into cryopreservation of these roles since the freshwater section of fish embryos’ (University of Luton, UK). CCAP relocated to SAMS in 2004. I have My research has been in the areas of wide ranging interests including: cryopreservation of fish embryos, oocytes Biological Resource Centres (BRC), and cells. My current major grants are cryopreservation, cryoinjury, algal stress ‘Development of technologies for physiology, algal biotechnology, cryopreservation of fish oocytes’ (funded application of molecular method in culture by the EU) and ‘Investigation of new collections and bioinformatics. approaches to the cryopreservation of fish embryos’ (funded by The Wellcome Ryan Cripps Trust). I have been a member of Society (firstname.lastname@example.org ) for Cryobiology since 1992 and a member I first dabbled with low temperature of Society for Low Temperature Biology biology as a tool to maintain enzyme, since 1993. I was the General Secretary carbohydrate and plant growth regulator (1997-1999) and the Treasurer (2001- activity/levels in plant tissue until analysis. 2004) of the Society for Low Temperature Subsequently my direction has changed a Biology. I have been a member of the little ... and now I am using low editorial boards of Animal Reproduction temperatures as a method to store Science (present) and Cryo-Letters (2000- vegetative tissue for the long-term 2003). I am also a referee of numerous conservation of rare and endangered specialist journals including Biology of plants. This research at the Royal Botanic Reproduction, Cryobiology, Garden, Kew (www.kew.org) is Theriogenology, Journal of Zoology, complementary to the work done at the Aquaculture and Aquaculture Research. Millennium Seed Bank. Thus, my primary At present I am the Course Director of the focus is on plants that cannot be stored by MSc Cryobiology course at University of conventional seed storage. My initial work Luton. was on developing a protocol for fern gametophyte cryopreservation. From there John Day I have worked on numerous species from (email@example.com) several different plant groups, including I am Curator of the freshwater algal woody plants, herbaceous plants, (including cyanobacteria) and protozoan carnivorous plants, selaginellas, orchids, subsections of the Culture Collection of ferns and mosses. I am currently working Algae and Protozoa (CCAP) at the on a number of plants from Great Britain, Scottish Association for marine Science Seychelles, Madagascar, St Helena and (SAMS) near Oban in Scotland. I was the Australia. Coordinator and Work Package (WP) leader of the recently completed COBRA Project www.cobra.ac.uk, which focused Andy Wetten on cryobiology and cryopreservation of (firstname.lastname@example.org) microalgae and cyanobacteria. In addition, I am interested in cryopreservation as a I am a reviewing editor for In Vitro tool for long-term conservation of plant Cellular Developmental Biology and am germplasm and the fundamental cellular on the editorial board of CryoLetters. I changes that limit the success of such previously served on the SLTB Committee procedures. Clonally propagated species from 1999-2002 and acted as General and those bearing recalcitrant seeds are Secretary from 2000-2002. being investigated, with particular 3. Wilkinson, T., Wetten, A., Prychid, C. reference to rare and endangered forms and Fay, M.F. (2003) Suitability of (1). TEM studies of shoot tips conducted cryopreservation for the long term storage with collaborators at RBG Kew have of rare and endangered plant species – a revealed the nature of subcellular changes case history for Cosmos atrosanguineus. during encapsulation/dehydration- Annals of Botany 91: 65-74. mediated cryopreservation and indicated that survival of only limited regions of the meristem are necessary to allow recovery of these propagules. Through X-ray diffraction analysis of encapsulated The SLTB Questionnaire somatic embryos, the effect of the 18 lessons to be learned dehydration procedure on membrane phase changes is being assessed while Purpose: The questionnaire was to find DSC is being used to determine the out what our members expect and what relationship between optimal survival and they would like to see the SLTB cytoplasmic vitrification in these tissues. Committee do and how the Committee can achieve this. Now that my group has developed an Material and methods: The questionnaire effective cryopreservation system to was prepared with input from all members support the University’s cocoa quarantine of the last Committee (term ended 29th facility (2) we are concerned with September 2005) and contained 21 assessing the effectiveness with which questions. It was sent out in printed form somaclonal variation (genetic mutations twice to the entire membership (112) exhibited as a result of the tissue culture together with the April and August 2005 process) can be prevented. AFLP-based newsletters and there were reminders analysis has revealed that cryopreserved about it in the 'Stop Press' on the front apical meristems of Cosmos page of both these newsletters. It was also atrosanguineus generally give rise to placed on the website where it still can be genetically true-to-type plants (3) while found: somatic embryo-derived plants have http://www.sltb.info/PDF/Questionnaire.P recently been found to be prone to DF. To keep things simple, the significant levels of variation. percentages given below are always Microsatellite-based analysis has calculated on the basis of the total number demonstrated significant levels of of returned questionnaires, NOT on the chimerism in cocoa somatic embryos and number of responses to a particular the technique is now being employed to question/option. determine how such mutation frequencies Results: A total of 23 questionnaires were are influenced by periods of returned to the Secretary, which cryopreservation. Current projects include corresponds to 20.5%. It is difficult to call collaborative work with RHS Wisley on this a representative sample (irrespective the preservation of the national of the kind of statistics applied) because Chrysanthemum collection and with the the characteristics of those people University’s School of Pharmacy on the returning the questionnaire is unknown. maintenance of clonal plant lines for use The majority of those who responded (17 in therapeutic drug development. out of 23 = 77.3%) have been members of the Society for more than 10 years. The 1. Wilkinson, T., Wetten, A. and Fay, M. main reason for becoming an SLTB (1998) Cryopreservation of Cosmos member was to develop contacts, followed atrosanguineus by a modified by 60.9% for attending SLTB meetings encapsulation/dehydration method. Cryo and 39.1% to receive the newsletter. One Letters 19, 293-302. person liked the large plant component 2. Fang, J-Y, Wetten, A. and Hadley, P. unlike the Society for Cryobiology. The 3 (2004) Cryopreservation of cocoa leading professional and research interests (Theobroma cacao L.) somatic embryos were “cells”, “cell banking” and for long-term germplasm storage. Plant “conservation” (all 47.8%), followed by Science 166: 669-675. “tissue banking” and “gene banking” (both 39.1%). On the other hand “organ banking” and “fungi” (both 21.7%) were Discussion: From the low return rate the “leaders” regarding “no interest” in (20.5%) it is clear that SLTB members do this category. According to the returned not like questionnaires or have better forms the SLTB is “relevant” to the work things to do than to reply to them (lesson of 95.6% of our members, for 47.8% it is 1). SLTB members like to make contacts even “highly relevant”. The organization (which we would expect so this is not a of annual meetings is “highly relevant” for lesson to be learned). Interest in meetings 69.6%, regular newsletters are “highly exceeds the interest in regular information relevant” for 56.5% and the website gets via newsletter (lesson 2), so this tells us 34.8% in this category. One person something about where to put our efforts commented that to “share scientific in the future. Lesson 3 is that the interest information and to have a few beers” is the stops at a certain size of the research topic: main task. All who returned the whereas “cell banking” and “tissue questionnaire have attended at least one banking” are “in”, “organ banking” is not. SLTB meeting, and 26% attend them Although the relevance of the SLTB for regularly. Nobody gave a reason why our members’ work (according to the he/she has not attended a meeting. Seventy returned questionnaires) is 95.6%, 79.5% percent think that the number of keynote do not consider the SLTB relevant enough lectures at the AGMs is sufficient, 65.2% to return the forms (lesson 4). The think this about the free oral presentations, importance of our main tasks can be 73.9% with regard to presented posters but ranked as follows: annual meeting > only 39.1% say this about workshops. newsletter > website (lesson 5). Those Two people wish to see sessions on “low who have returned the questionnaire have temperature adaptations” at future SLTB attended at least 1 SLTB meeting, so we meetings, other topics (total of 12 different should set up more meetings to get more topics, e.g. “assisted reproduction”, questionnaires returned (lesson 6). SLTB “disease transmission and cryostorage”, members are able to think logically “natural cold hardiness) were proposed (lesson 7): as all of those who have only once. Nobody felt that industry had returned their form have attended at least 1 too high an influence on SLTB activities meeting, nobody gave a reason why he/she or the program of scientific meetings. did not. Nobody said that we have too Some 52.1% considered our website to be many keynote lectures, free oral “average” with regard to the quality of presentations, workshops and posters information, and 60.9% think this about during AGMs (lesson 8). As there is no the form of presentation. This analysis is outstanding topic of common interest for based on 2 people who visit it once a future sessions at AGMs (lesson 9), how week, 5 who do this once a month and 13 about combining several of them into one who do this every 3 months. Two people session (e.g. “Disease transmission by visit the website once a year. There were 6 assisted reproduction as an alternative to proposals for additional features to be low temperature adaptation or cold shown on the website, one being the hardiness”)? Nobody thought that the “publication of the month”. influence of industry on SLTB activities, Sixty five percent knew that the SLTB can including our meetings, is too high (lesson claim back taxes from the membership 10); how about changing this to allow for dues of UK taxpayers. Three people more free beers during the banquets (and admitted that they did not know that the for the Committee, more worldwide SLTB newsletter can also be found on the travelling and staying at better hotels)? As website. One third (7 people) did not know the form of presentation on the website that the SLTB constitution and the minutes (52.1%) is ranked slightly better than the are posted on the website. A Euro bank quality of the information provided account should be opened up to facilitate (60.9%), we should think about changing payments for members in the Euro zone this by making the presentation worse according to the opinion of 82.6%. (lesson 11). We should not update our Nobody judged the availability of the website too frequently (lesson 12), as SLTB committee for comments and otherwise people would miss something remarks to be poor, all votes ranged during 2 successive visits. As one proposal between average and excellent. was to add a “publication of the month” to the website, we have now identified one person who will help us (lesson 13). We only have to find out who this person is and they can then read all the relevant Life-down-under: a bunch of wild literature and make a proposal (additional flowers volunteers welcome). People seem to like to support the UK tax collection (lesson The city of Perth in Western Australia has 14), as they know that we can claim back been a media article for visiting taxes from member dues of UK taxpayers personalities, such as, Cherie Blair during but (almost) nobody sends back the form ‘charitable engagements’ but it is also a which can be downloaded from our beautiful city that played host this website (http://www.sltb.info/forms.html, September (21-24) to the Conference of click on “Gift Aid Form”). Although the Australian Branch of the International everybody said that he/she has already Association for Plant Tissue Culture and visited the website, 3 people did not know Biotechnology. The theme ‘Contributing that the newsletter could be found there… to a Sustainable Future’ was explored in The good news is that if we removed the the newly built Ecology Centre of the newsletter from the website only 13% Botanic Gardens and Parks, in Bold Park, would not realize that (lesson 15). We which is a beautiful bush reserve proved to could inform at least 7 more people about be an ideal location for 3 days of papers the fact that the SLTB constitution and the and posters. The conference was a minutes of the AGMs are posted on the showcase for recent developments in plant website. Lesson 16 is that it is a tough job tissue culture and biotechnology, to spread information among SLTB highlighting contributions to sustainability members. Although the broad majority in horticulture, agriculture, forestry, and (82.6%) welcome the idea to open up a conservation of the natural environment. EURO bank account, this will not be done Plenary lectures to address issues in because of the cost in relation to the small germplasm preservation: cryopreservation number of “continental” European SLTB were given by Dr Erica Benson, members who would benefit from this. We University of Abertay, Dundee, Scotland; will have to wait until the EURO is Legume biotechnology by Dr Sergio introduced in the United Kingdom (lesson Ochatt, Laboratoire de Physiologie et 17): “Fog over the Channel, the Continent Culture in Vitro Dijon, France; plant tissue cut off.” I am very glad to hear that the culture past, present and future by Prof availability of the SLTB committee for Acram Taji, University of New England, comments was judged from average to Armidale NSW Australia and aspects of excellent by 100%. sustainable agriculture by Dr Philip Conclusion: The explanation for the poor Davies, South Australian R & D Institute, return rate of the questionnaire (in my Adelaide SA Australia. opinion) is that almost nobody needed this Like the efforts in Australian cricket, the way of communication (lesson 18, the social program got off to a flying start most important one)! with the welcome registration function in Those of you who are interested in the the palatial Ocean Room of the complete set of data may wish to contact Rendezvous Observation City Hotel, in the me or our past secretary Paul Lynch beachside suburb of Scarborough - homely (email: email@example.com). Paul has reminder to English guest speakers. Perth compiled the data and also written up all enjoys a warm to hot, dry summers and proposals made regarding what would mild winters but in September, make SLTB membership more attractive temperatures range from 10-20° Celsius for new people (question 19), what would and those hardy souls did enjoy swimming help the SLTB to keep existing members at Scarborough beach. There were some (question 20), and any other remarks on 60 delegates covering a wide range of oral the SLTB (question 21). A copy of Paul’s and poster presentations from “result summary” has been forwarded to cryopreservation to ecological ‘hot spots’ the new SLTB chairperson. Good luck in global biodiversity, where Professor Tiantian and the new Committee! Richard Williams (University of Queensland) delivered the Mike Mullins Andreas Sputtek Memorial Lecture during the conference. Past Chairman In 1999 the Australian branch of the As with other collections of biological IAPTC&B established the Ron de Fossard materials, algal culture collections have award to celebrate excellence in plant two key functions: they provide tissue culture and honour the contribution consistency and quality, traceable source made to the field by Ron de Fossard, a material and information. They are, de Life Member of the Association. Parallel facto, sources of biological standards and to the champagne-cork popping activities without these standards comparative in Trafalgar Square following the Ashes taxonomic, physiological, ecotoxicological victory, the conference dinner was held in and ex situ ecological studies are the Boatshed Restaurant, Coode Street impossible or, at best, problematic. The Jetty, South Perth a spectacular location value of collections to the user community overlooking the evening illuminations of is widely recognized, not least in that they the downtown district. During these provide cultures that are often difficult or celebrations this inaugural award at the virtually impossible to re-isolate from conference in Perth was given to Prof their natural habitats, even when the Acram Taji for her outstanding original source is known and accessible. contribution to plant tissue culture. The They provide a store of characterized conference concluded with a Spring diversity for research/ exploitation, or seasonal upturn in the weather during the potentially, particularly in the case of wildflower field trip along the Darling macroalgae, reintroduction to their Scarp outside Perth that allowed some rare original habitats. The holdings of algal Western Australian blooms to open. All- collections are extremely diverse and it is in-all it was a splendid conference the aim of the service collections to make organized by the chair Jen McComb and available and conserve ex situ as much of her organizing committee, a timely the great morphological and genetic meeting in a beautiful location and venue biodiversity of microalgae as possible. over-layered with scientific excellence! For phylogenetic and taxonomic studies Further details and references can be taken authentic strains, i.e. cultures derived from from source ‘Contributing to a Sustainable the material used for the original Future’ IJ Bennett, E Bunn, H Clarke, JA description and naming of the species, are McComb (Eds.) Proceedings of the particularly useful - these and the vast Australian Branch of the IAPTC&B, literature published on the algal culture Perth, Western Australia, 21-24th collections’ holdings make them September, 2005. Published by: The irreplaceable and unique international Australasian Plant Breeding Association resources. Inc. ISBN 0-9581784-1-0 In Europe today there are debatably more protistan collections than at any time over Keith Harding the past century. The first algal culture collection meeting to be held in Europe “Culture Collections of Algae: Increasing Accessibility and Exploring Algal Algal culture collections in Europe: Biodiversity”, held at the Sammlung von The need to use cryopreservation Algenkulturen (SAG) at the University of Göttingen, Germany, September 2-6 2002 European microbial culture collections highlighted the valuable contributions of have provided a service to the scientific culture collections to science (Surek community for over a century, with the 2002). This meeting was attended by first “service collection” established by Dr representatives from 19 European Franticek Král in Prague towards the end collections, from 14 European countries as of the nineteenth century. In the 1920’s well as representatives from algal Prof E.G. Pringsheim developed the first collections in North America and Asia. It major protistan culture collection in also highlighted the diversity of European Prague publishing its first catalogue in collections and the wide range of skills 1928 (Pringsheim 1928). This collection associated with these collections. At this has subsequently become the “parent”, or meeting there was a clear consensus that even “grandparent”, of most of the largest there was a need to improve accessibility algal culture collections world-wide (Day to biological resources at a European level et al. 2004). and to increase cross-collection collaboration. As a component of the Algi- has resulted in the largest collection of its Net project www.search- type in the world and currently in excess labs.com/Alginet/, a survey of collections of 3000 cultures are held in a and their holdings is ongoing. To date, this cryopreserved state (see previous SLTB has identified the existence of 9 macro- Newsletters, or www.cobra.ac.uk for algal culture collections and 90 micro- further details). algal culture collections. These collections It is hoped that the foundations laid by the vary in size from small personal academic Algi-Net and COBRA projects can be research collections holding a “handful” of expanded upon and it is clear that cultures, biotechnological collections to cryopreservation will remain a cornerstone well established service collections of ex situ maintenance of protistan including: the Pasteur Culture Collection biodiversity. of Cyanobacteria in Paris (France), , Culture Collection at the Botanical References Institute in Trebon (Czech Republic), DAY, J.G., J. LUKAVSKÝ, T. FRIEDL, NIVA Collection (Norway), ACOI J.J. BRAND, C.N. CAMPBELL, M. Coimbra Collection of Algae (Portugal), LORENZ & J. ELSTER (2004) SAG (Germany) and CCAP (UK). In total Pringsheim’s living legacy: CCALA, the holdings of these collections are in CCAP, SAG and UTEX culture excess of 16,000 algal strains and collections of algae Nova Hedwigia 79: undoubtedly represent the most 27-38. comprehensive range of algal cultures in PRINGSHEIM, E.G. (1928): the world. It is planned to improve access Algenreinkulturen. – Ber. Deutsch. Bot. to the holdings of these collections via the Ges. 46: 216-219. Algi-Net website and in due course the SUREK, B. (2002) Review of “Culture findings will be made available on-line at Collections of Algae: Increasing www.search-labs.com/Alginet/. One Accessibility and Exploring Algal aspect of the project is to highlight how Biodiversity” meeting. Protist 153: 343– the roles of these collections, particularly 355. the larger service collections, has expanded past the traditional curatorial role and include services such as patent deposit facilities and the supply of cultures for quality control. Indeed, service culture collections have evolved into Biological Resource Centres (BRC’s) thereby responding to revolutionary developments in areas such as molecular biology and bioinformatics. The current role of BRC’s is to provide the scientific world with access to properly maintained culturable material, in the case of algal collections with cyanobacterial and protistan cultures and their associated data. Because of concerns over strain stability and the logistical/ cost implications of maintaining large numbers of cultures using serial JG Day & R Saxon transfer, in recent years, there has been CCAP, Scottish Assoc for Marine Science increased interest in the application of long-term preservation methods to algae. This was one of the drivers of the COBRA project (The COnservation of a vital european scientific and Biotechnological Alas farewell to CRYMCEPT Resource: microAlgae and cyanobacteria), where a pan-European Biological Society members will recall a feature in Resource Centre containing cryopreserved the April 2004 newsletter regarding algae was developed based on existing CRYMCEPT algal culture collections (Table 1). This (http://www.agr.kuleuven.ac.be/dtp/tro/cry mcept/) – an acronym for a European techniques to existing European Commission Research Project (QLK5-CT- germplasm collections and research 2002-01279) to Establish institutes via the workshops. CRYopreservation Methods for Integral to this projects’ evaluation - is the Conserving European PlantT Germplasm exercise in the preparation of reports. The Collections. This project was funded by partners within the steering group received the EU’s Fifth Framework Programme for a briefing session on the reporting Quality of Life and Management of Living requirements for the CRYMCEPT project Resources: Key action 5 ‘Sustainable to the European Union, as specified in the agriculture, fisheries and forestry, and EU document on guidelines for the started in November 2002 for three years. preparation of periodic and final reports. Although, it seems like yesterday, the 3 The reports to be prepared include: (1) a years are now up and the project has report for the 4th Steering Committee reached its conclusion, this culminated in meeting; (2) a periodic report; (3) a final the final steering committee meeting in report; (4) a Technological Montpellier, France and two jointly Implementation Plan (eTIP) and (5) cost organised CRYMCEPT and IPGRI statements for the project. Highlighting the cryopreservation training workshops for most important report to prepare was the plant germplasm. One was held in Leuven, final report, which will provide the Belgium (12-22 Sept and the other in detailed scientific results presenting the Montpellier, France 10-21 Oct). objectives, work and results achieved The 4th Steering Committee of the during the lifetime of the project. Once CRYMCEPT project took place at the these reports are complete and following International Plant Genetic Resources approval, like the COBRAL issue (Aug05 Institute, INIBAP office in Montpellier, newsletter) a summary reporting the France (7-8th October). It brought together outstanding scientific achievements will the project partners for the last time before be a feature for the next newsletters. the end of the project. Richard Markham, The two workshops targeted germplasm Director of the IPGRI/INIBAP Office collection holders in Europe, as well as welcomed participants and gave an countries from the EU international co- overview of the IPGRI’s programme on operation programme; each was attended commodities crops, (particularly banana, by some 15 representatives of member cocoa, and coconut) for livelihood and states mainly from across Europe, but also sustainable activity. Each CRYMCEPT had representatives from Africa, Asia and partner gave a presentation of their work Latin America. The theme was to address performed in the different work packages the urgent need to develop in which they were involved during the cryopreservation techniques for important final year of the project. These were plant genetic resources, which otherwise followed by a discussion of each work could not be adequately conserved using package and a general discussion of the conventional methods. The workshop(s) main results achieved and the lessons task was to disseminate the results of the learnt in the overall project. Overall the CRYMCEPT research to develop optimal project has been a huge success with many cryopreservation protocols for a number of scientific achievements both published and plants such as garlic, olive, Ribes, apple, those to come. As with most projects, the almond, potato, banana and coffee. After realisation of the true magnitude of the an almost 2 week exhausting exercise, the efforts during the 3 years is often seen remaining participants received their after its completion in the total volume of certificate for training in cryopreservation outputs. These productions cover a range and departed home delighted with the of subjects: from the thermal behaviour of cooperation, scientific information and water; protein function; understanding outcome of the Workshop. An apt sugars; membrane components; the conclusion to end what was a very significance of polyamines; cytoskeletal enjoyable EU-project, in sowing the seeds proteins; the induction of oxidative stress; for the next generation in plant all leading to the development of new cryopreservation. cryopreservation protocols, and finally the dissemination of improved cryopreservation protocols and analytical Management Committee Members COST CRYO-Action Programme (MCM) in Brussels on January 2004, to 2005… organize joint final meeting with participation of all working group (WG1, There are many words said within a WG2, WG3) and MC members in Stara working day, and the word ‘cost’ can stir a Lesna, Slovakia for the final COST 843 variety of images, as it flickers across the conference, as the COST Action was due memory ingrams but in 1970s it was the to end in 2005. beginning of a bold scheme to establish As we have seen, all EU research scientific links throughout Europe. programmes come to an end, however Founded in 1971, COST is an inspired members of the complete intergovernmental framework for CRYMCEPT and COBRA projects and European Co-operation in the field of the previous COST programme have Scientific and Technical Research, agreed to conspire against the ‘lack of allowing the co-ordination of nationally funding’ for cryoconservation and have funded research on a European level prepared a preliminary proposal for a new (http://www.cost.esf.org/index.php). cost action on plant cryopreservation Highly relevant to SLTB and members mainly dealing with networking, involved in the cryopreservation of plant organization of yearly meetings, short germplasm, a more recent COST action term lab-training sessions. Surprisingly, 843 deals with various aspects of plant the initial EU response to this initiative production through tissue culture, and has has been very positive for the submission two main objectives: innovation of plant of more complete proposal in the subject propagation methods, and plant quality of plant cryopreservation. Although, there enhancement – key issues in the success of are still many hurdles to jump before the plant cryopreservation. COST 843 is final outcome, this is truly encouraging organised in three Working Groups news, as this funding and subsequent dealing with the following topics: WG 1, networking will be a major factor in the Developmental biology of regeneration; development of scientific advances for WG 2, Advanced propagation techniques; cryopreservation. WG 3, Assessment of performance: It is fair to surmise, that the proposal is in physiological health and (epi- )genetic the ‘draft stage’ and this allows the stability (http://www.cost843.org/) opportunity to invite members of the Although, cryopreservation was SLTB (and other plant related colleagues) considered a minor activity in this COST that if you are interested to join this action, it was generally recognized that a proposed COST ACTION, and would like prerequisite for the development of new to play an active role or would like more propagation-cryopreservation techniques information to contact the ACTION is the fundamental knowledge about the coordinator Bart Panis regeneration process. In all Working (firstname.lastname@example.org) or Keith Groups, there were components with Harding (email@example.com). molecular, biochemical, physiological and histological studies on adventitious regeneration of shoots and embryos regarding aspects of manipulation of Cryopreserving Chrysanth’s competence to regenerate, induction and maintaining of embryogenic callus, study Chrysanthemum cultivation began in of genes involved in regeneration China and is described in writings as early (transformation or differential expression), as the 15th Century B.C. As well as their (cryo-) recalcitrance and rejuvenation. The use as the source of one of the most papers in the issue (Plant Cell, Tissue and important naturally occurring insecticides, Organ Culture 70: 123, 2002) are based on chrysanthemums also constitute a major selected presentations given during the part of global cut flower sales. The first meeting of Working Group I of National Chrysanthemum Society (NCS) COST action 843, held from 12 to 15 is looking to arrest the gradual October 2000 at Geisenheim, Germany. disappearance of old varieties of the plant As the COST action proceeded, on one through a new collaboration with the occasion, it was decided during University of Reading’s School of Biological Sciences. Meristems are being transplantation will also be covered. The targeted for propagule storage through format will be the “classic” one, i.e. a encapsulation/dehydration with the first reception on the evening of the day regenerants exhibiting the additional preceding the meeting, followed by a four benefit of virus elimination. day scientific meeting. On the first day David Pegg will organize a symposium Andy Wetten (working title: “Clinical Applications of Low Temperatures”). On the morning of the second day we will have lectures on plant low temperature biology organized Interesting research tool by Dirk Hincha. In the afternoon there will be a guided bus tour of Hamburg, and a The Postgraduate School at Writtle combined barbecue/canoe event. On the College will take delivery of the first UK- third day Ursula Rauen has agreed to installed Sperm Vision™ system just organize lectures on the topic of before Christmas. Delivered by MTG – “hypothermia”. The traditional banquet Medical Technology Vertriebs-GmbH, the will take place during the evening of the system provides digital-image technology last day. to track, record and analyse a wide range of sperm motility and motion Deadlines characteristics, monitoring up to 95 spermatozoa in a single field. The Early registration: Before 1st June 2006. integrated database allows motility A “Call for Abstracts” will go out in information to be compared, immediately, January 2006. against previously recorded information. Abstract submission: Until 1st May 2006. Our immediate use for this system is in The registration form and the form for studies of cryopreservation of horse and abstract submission will be available on ram sperm, but we are keen to collaborate the web by the end of January 2006. on other projects involving frozen semen. Please watch the meetings website at We are also interested in using the http://www.CRYO2006.org for further potential of the system for monitoring the details. post-cryo performance of other, motile cells such as unicellular algae and Notification of abstract acceptance: 31st protozoa and would be pleased to discuss May 2006 possible collaborations with any of you that are interested. Accommodation: To be announced, see website. Brian Grout Andreas Sputtek, Chairman CRYO 2006 Organizer CRYO 2006 Hamburg 24th–27th July From the Treasurer The meeting will be held as the 43rd Annual Meeting of the Society for As the current year draws to a close it is Cryobiology in association with the SLTB my job as Treasurer to prompt you to think and will cover a wide range of subjects about a New Year resolution to pay your including hypothermia, physiology of 2006 subscription as soon as possible resistance to cold in plants, and [indeed, there are a notable few of you applications of cryobiology in who have already done so!]. This might conservation, surgery, cell, tissue and also be the time to ask the flagging few organ preservation. Relevant aspects of who have yet to pay for 2005 to clear their biology, molecular biology, physics, conscience by wrapping this payment up chemistry, physical chemistry, with 2006. biochemistry, physiology, medicine, transfusion medicine, mechanical Our current charges are £20 for standard engineering, tissue engineering and membership and £15 for students. For UK members a Standing Order Gift Aid Declaration through a current bank account is the most efficient and convenient way to make your As the Society is now a registered UK payment. The necessary form is available charity, we can reclaim the income tax on at www.sltb.info/forms.html, or I will mail UK members’ subscription payments from one to you if that is easier. If you are an the Inland Revenue. To do this, we need established Standing Order user then, members to declare, formally, that they please would you ensure that this has been agree to the Society treating their adjusted to the current rate. subscriptions as Gift Aid donations. This Alternatively, payment can be made by is done by completing a Gift Aid cheque [increased to cover any service Declaration form (on the website at charges], made payable to the Society, and www.sltb.info/forms.html) and will sent directly to me, Brian Grout-SLTB greatly help the Society’s balance sheet! Treasurer, Postgraduate School, Writtle College, Chelmsford CM1 3RR, UK. Brian Grout, Treasurer At present the Society is unable to accept payment by credit or debit card and so international members will have to use an Note: The material for this edition was electronic money transfer. prepared by John Day. SLTB Officers and Committee 2005-2006 Chairman Tiantian Zhang Institute of Research in the Applied Natural Sciences University of Luton, The Spires, 2 Adelaide St Luton LU1 5DU, UK Tel: +44 (0)1582 743729 Fax: +44 (0)1582 743700 Email: firstname.lastname@example.org General Secretary Meetings Secretary John Day Keith Harding Culture Collection of Algae & Protozoa Plant Conservation Group Scottish Assoc for Marine Science School of Contemporary Sciences Dunstaffnage Marine Laboratory University of Abertay Dundee Dunbeg, Argyll Kydd Building, Bell Street PA37 1QA, UK Dundee DD1 1HG, UK Tel. +44 (0)1631 559000 Tel. +44 (0)1382 308535 Fax: +44 (0)1631 559001 Fax: +44 (0)1382 308261 Email: email@example.com Email: SETKH1@abertay.ac.uk Treasurer Brian Grout M. Elena González Benito Director of Postgraduate Studies Dpto de Biología Vegetal Postgraduate School Escuela Univ de Ingen Técnica Agrícola Writtle College Universidad Politécnica de Madrid Chelmsford CM1 3RR Ciudad Universitaria, 28040 Madrid UK SPAIN Tel: +44 (0)1245 424200 Tel: +34 91 336 54 35 Fax: +44 (0)1245 420456 Fax: +34 91 336 54 06 Email: Brian.Grout@writtle.ac.uk Email: firstname.lastname@example.org Ryan Cripps Andrew Wetten Micropropagation Unit School of Biological Sciences Royal Botanic Gardens, Kew Plant Science Labs, Univ of Reading Richmond TW9 3AB Whiteknights UK Reading RG6 6AS, UK Tel: +44 (0)20 8332 5559 Tel: +44 (0)118 931 6380 Fax: +44 (0)20 8332 5524 Fax: +44 (0)118 378 8160 Email: email@example.com Email: firstname.lastname@example.org Compiled by Belinda Wade, Medical Cryobiology Unit, Department of Biology, University of York, UK.
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