Prior risk assessment for a technique involving the use of by maclaren1


									                                                                        MRC Clinical Sciences Centre
                                                                  Imperial College Faculty of Medicine
                                                                               Hammersmith Campus
                                                                                       Du Cane Road
                                                                                    London W12 0NN


                                           Technique title
       Cutting out DNA bands from an Agarose Gel

                                            Staff involved
       All Staff in room……………….

                                          Hazards involved
       Chemicals: Ethidium Bromide,
       Possible electric shock
       UV light burns
       Sharps injury

                                         Route of exposure
       Skin, eyes, ingestion, injection (from sharps injury)

       Chemicals: The respective COSHH forms for the above substances must be read prior to
        this technique commencing so that their hazards and control measures are understood.
       Electric shocks: can burn and in some cases kill, especially when used near liquid or if
        faulty equipment is used.
       UV light: Can burn skin as well as damage the eyes. Repeated exposure could lead to
        irreversible effects.
       Sharps: Razor blades/scalpels can cut very deeply and can also introduce contaminants
        into a wound

 Identify any persons and any special risks associated with the technique and steps that
                           must be taken to minimise them.
       Person carrying out the work: All chemicals, reagents and associated techniques used in
        this protocol will have their associated risks and control measures so their relevant COSHH
        forms should be read in conjunction with this protocol. UV can burn exposed skin, the face
        and damage the eyes. Power packs can potentially cause electric shocks. Scalpels and
        razor blades need to be used very carefully.
       Other Persons: Small risk of exposure to chemicals from equipment door handles etc.
        Small risk of UV burn if they are too close to the gel imager while it is switched on.
        Electrical hazard from the gel tanks and power packs. Risk of sharps injury if scalpels etc.
        have not been disposed of properly. .

       Controls 1: All new staff and students have a laboratory induction to ensure that they
        understand all of the safety considerations associated with the work carried out within this
        laboratory. All techniques are explained and demonstrated to all new staff and students.
        Staff are sent on the appropriate training courses offered by the MRC to ensure they are
        familiar with all aspects of good laboratory practice.
       Controls 2: Appropriate PPE is always worn as well (Nitrile or Latex Gloves and Lab coat
        etc. shields are also worn when working with UV light) All laboratories have fully functional
        fume cupboards that are used when working with toxic or volatile chemicals). No eating
        and drinking is allowed in CSC labs either so there is no real risk of ingestion of chemicals.
        All sharps are disposed in Sharpsafe and these are disposed of when full through the
        clinical waste route.

Risk Assessment Form for Laboratory Procedure                                             1
MRC Clinical Sciences Centre
Version 3 July 09
                                                                        MRC Clinical Sciences Centre
                                                                  Imperial College Faculty of Medicine
                                                                               Hammersmith Campus
                                                                                       Du Cane Road
                                                                                    London W12 0NN

                                  Emergency Spillage Procedure
       All chemicals in the protocol will have the relevant spillage listed in its own COSHH form
        and all staff should familiarise themselves with these procedures.
       Ethidium Bromide contaminated gels could be dropped. If so, pick up and wrap in tissue
        paper and dispose of into one of the special EtBr bins in the department. This waste will
        then be disposed of via the “incineration route”. Wash the affected area with detergent and
        then monitor with the hand-held UV lamp.
       All accidents must be reported to the Lab Manager / Safety Supervisor or the
        departmental first aid personnel, and CSC Safety via an accident report form:

                                           Waste Disposal
       Details of the proper means of disposal of chemical waste can be found in individual
        COSHH forms.
       Tips eppendorf tubes etc. should all be disposed of as clinical waste.
       Ethidium Bromide solid waste needs to be placed into the special “for incineration only” bin
       Ethidium Bromide liquid waste needs to be cleaned up either using the purification column
        or “Tea Bag” method.

                     Include a brief stepwise description of the technique
       Put on your laboratory coat and gloves
       Collect your prepared agarose gel (See ”RA for pouring an agarose gel’)
       Run your samples on the agarose gel and visualise the bands (See “RA for loading &
        visualising DNA bands on an agarose gel’ RA)
       When your DNA bands have run far enough down the gel and you have taken a picture as a
        record of the experiment, double contain the gel and take it to the darkroom along with an
        eppendorf for your sample, a razorblade and some tissues.
       Switch on the white light, close and lock the darkroom door
       Put on one of the face guards
       Switch on the UV trans-illuminator and position your gel on the surface
       Try to keep the amount of time the gel is exposed to UV at a minimum to prevent
        unwanted UV-induced DNA damage to the DNA
       Switch off the white light
       Identify your DNA band of interest and carefully cut it out of the gel using the razorblade.
       If you do cut yourself, follow the „sharps‟ procedure as below...

        Cover the wound and switch off the UV trans-illuminator
        Immediately rinse the cut under running water and encourage bleeding for 30-
        Get a plaster from either LRB14 or 4S4 and cover the wound.
        Let your laboratory supervisor know about the accident.
        Notify the safety officer and fill out an accident report.
        If it is a particularly bad cut then go straight to the A&E department of the

       Put our gel band into the eppendorf and seal the lid
       Repeat for all the samples that are required
       Switch on the white light
       Switch off the UV trans-illuminator
       If finished with the gel, discard it in the yellow clinical waste bag marked “for incineration
        only” or return it to the gel tank to run the samples out further.
       Wipe down all surfaces with the tissue and discard in a yellow clinical waste bag

Risk Assessment Form for Laboratory Procedure                                            2
MRC Clinical Sciences Centre
Version 3 July 09
                                                                         MRC Clinical Sciences Centre
                                                                   Imperial College Faculty of Medicine
                                                                                Hammersmith Campus
                                                                                        Du Cane Road
                                                                                     London W12 0NN

                        Include a brief stepwise description of the technique
         Open the darkroom door and switch off the white light.

 Due consideration should be made in this assessment for women of child-bearing years, and a full
               re-assessment should be carried out upon notification of pregnancy.

    This assessment has been carried out by the person responsible for the work. A copy of this
                            assessment has been sent to CSC Safety.

RESPONSIBLE PERSON                         NAME                      SIGNATURE                DATE

Person carrying out the

 Principal Investigator

 Group Safety Supervisor

        Annual Review

Risk Assessment Form for Laboratory Procedure                                             3
MRC Clinical Sciences Centre
Version 3 July 09

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