inform May 2009, Vol. 20 (5) 323
shapes within 12 min. Overall recoveries injection-port silylation by GC–MS, good E-EPA group without MDE (n = 46) and
ranged from 88 to 119%, with detection linearity of analytes was achieved in the the placebo group (n = 45) were 8.0 (95%
limits for SFE ranging from 2 to 30 μg L−1. range of 0.02–10 ng/mL with coefficients CI: 0.6, 15.3; P = 0.034) for the PGWB,
The linearity as correlation coefficient was of determination, R2 > 0.995. Good repro- –0.2 (95% CI: –0.01, –0.4; P = 0.040) for
higher than 0.99 except for pregnenolone- ducibility was obtained with relative stan- the HSCL-D-20, and –2.7 (95% CI: –0.3,
3-arachidate (r2 = 0.98) in the concentration dard deviation less than 19.6%. The limits –5.1; P = 0.030) for the HAM-D-21. Differ-
range of 5–3000 μg L−1. Ten serum samples of detection ranged from 1.3 ng/mL to 15 ences in adjusted 8-wk changes between the
obtained from volunteers were also analyzed ng/mL (S/N = 3) in environmental water E-EPA group with MDE (n = 13) and the
and DHEA (dehydroepiandrosterone)- samples. Compared with traditional off- placebo group (n = 16) were not significant.
3-palmitate and pregnenolone-3-stearate line silylation of fecal sterols performed To our knowledge, this is the first trial of
were quantitatively determined as 1.8– with water bath (60°C, 30 min), this injec- n-3 supplementation in the treatment of PD
1195.8 μg L−1 concentration. The devised tion-port silylation method is much simpler and depressive symptoms in middle-aged
high-temperature GC–MS method could be and convenient. The developed method has women. In women with PD without MDE
useful for identification of SFE in biologi- been successfully applied for the analysis at baseline, the 8-wk changes in PD and
cal specimens including serum. of fecal sterols from real environmental depressive scales improved significantly
water samples. more with E-EPA than with placebo.
Sterol lipidomics in health and
disease: Methodologies and Ethyl-eicosapentaenoic acid for The relationships of cholesterol
applications the treatment of psychological metabolism and plasma plant
Griffiths, W.J., and Y. Wang, Eur. J. Lipid distress and depressive sterols with the severity of
Sci. Technol. 111:14–38, 2009. symptoms in middle-aged coronary artery disease
In this review we discuss the impor- women: A double-blind,
tance of sterols and their metabolites in Silbernagel, G., G. Fauler, W. Renner, E.M.
health and disease. The review extends placebo-controlled, randomized Landl, M.M. Hoffmann, B.R. Winkelmann,
from precursors of cholesterol to their ulti- clinical trial B.O. Boehm, and W. März, J. Lipid Res.
mate metabolites, i.e., bile acids. The mass Lucas, M., G. Asselin, C. Mérette, M.-J. 50:334–341, 2009.
spectrometric technology used for the anal- Poulin, and S. Dodin, Am. J. Clin. Nutr. Changes in the balance of choles-
ysis of these molecules is reviewed and the 89:641–651, 2009. terol absorption and synthesis and mod-
most modern methodologies highlighted. Psychological distress (PD) and erately elevated plasma plant sterols have
depressive symptoms are commonly been suggested to be atherogenic. Measur-
Determination of fecal sterols observed during menopausal transition. ing cholestanol, lathosterol, campesterol,