Analysis of fatty acids in sputum from patients with pulmonary tuberculosis using gas chromatography-mass spectrometry preceded by solid-phase microextraction and post-derivatization on the fiber

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Analysis of fatty acids in sputum from patients with pulmonary tuberculosis using gas chromatography-mass spectrometry preceded by solid-phase microextraction and post-derivatization on the fiber Powered By Docstoc
					                                                                                               322            inform May 2009, Vol. 20 (5)


       the platelets or of erythrocyte membranes,       Stearoyl-CoA desaturase and                     on the fiber coupled to gas chromatogra-
       yet these procedures are lengthy. Other                                                          phy–mass spectrometry (GC–MS) was
       investigators have used adipose tissue
                                                        its relation to high-                           developed for the analysis of fatty acids
       obtained by percutaneous biopsy, but it          carbohydrate diets and obesity                  in sputum from patients with pulmonary
       must be pointed out that this procedure is       Flowers, M.T., and J.M. Ntambi, Biochim.        tuberculosis. The sputum specimens were
       not only invasive but also time consuming.       Biophys. Acta, Mol. Cell. Biol. Lipids          digested, hydrolyzed, extracted, deriva-
       Also, it has to be noted that the turnover       1791:85–91, 2009.                               tized, injected, and analyzed without cul-
       time for fatty acids has been estimated to            Obesity is currently a worldwide           tivation or isolation of the microorganism.
       be 1–3 years.                                    epidemic and public health burden that          Under optimized conditions, the relative
                                                        increases the risk for developing insulin       standard deviations (RSD, n = 5) for all
                                                                                                        analytes were below 17%, and the limits
       Ruminant and industrial trans                    resistance and several chronic diseases
                                                                                                        of detection varied from 1.68 (C 24:0) to
                                                        such as diabetes, cardiovascular diseases,
       fatty acids: Consumption data                    and nonalcoholic fatty liver disease. The       150.4 μg L −1 (C 12:0). Good linearity was
       and health aspects. Is there a                   multifactorial causes of obesity include        observed for all the fatty acids studied
                                                                                                        except for C 12:0 within a wide concentra-
       difference?                                      several genetic, dietary, and lifestyle vari-
                                                                                                        tion range of three orders of magnitudes
                                                        ables that together result in an imbalance
       Grooten, H., and C. Oomen, Lipid Technol.        between energy intake and energy expendi-       with the correlation coefficients ranging
       21:36–38, 2009.                                  ture. Dietary approaches to limit fat intake    from 0.91 (C24:0) to 0.99 (C14:0). Fatty acids
            Consumption of trans fatty acids            are commonly prescribed to achieve the          in sputum specimens from 21 persons
       (TFA) has been associated with a higher          hypocaloric conditions necessary for            were directly analyzed using the pro-
       risk of coronary heart diseases. In our          weight loss. But dietary fat restriction is     posed method. The results show that in all
       daily food pattern two different sources of      often accompanied by increased carbo-           the sputum specimens from patients, who
       TFA can be distinguished: ruminant TFA           hydrate intake, which can dramatically          were clinically diagnosed with tuberculosis
       and industrial TFA. Controversy exists           increase endogenous fatty acid synthesis        (TB), tuberculosis stearic acid (TBSA) was
       between the health effects of both sources.      depending upon carbohydrate composition.        detected, while in all the sputum samples
       Now, new findings are available in which         Since both dietary and endogenously syn-        from persons without TB, TBSA was not
       both TFA sources are investigated in inter-      thesized fatty acids contribute to the whole-   found. The possibility of using the pro-
       vention studies. Also, an epidemiological        body fatty acid pool, obesity can therefore     posed method to detect mycobacterium
       study is published in which a wide intake        result from excessive fat or carbohydrate       tuberculosis (MTB) via the identification
       of ruminant TFA is investigated. These data 
				
DOCUMENT INFO
Description: A method based on solid-phase micro extraction (SPME) and post-derivatization on the fiber coupled to gas chromatography-mass spectrometry (GC-MS) was developed for the analysis of fatty acids in sputum from patients with pulmonary tuberculosis.
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